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Pistacia (Pistacia vera) hulls (PV) is a health product that has been determined to contain bioactive phytochemicals which have fundamental importance for biomedical use. In this study, PV ethyl acetate extraction (PV-EA) fractions were evaluated with the use of an MTT assay to find the most cytotoxic fraction, which was found to be F13b1/PV-EA. After that, HPTLC was used for identify the most active compounds. The antioxidant activity was analyzed with DPPH and ABTS tests. Apoptosis induction in MCF-7 cells by F13b1/PV-EA was validated via flow cytometry analysis and a distinctive nuclear staining method. The representation of genes like Caspase 3, Caspase 8, Bax, Bcl-2, CAT and SOD was assessed via a reverse transcription (RT_PCR) method. Inhabitation of Tubo breast cancer cell development was examined in the BALB-neuT mouse with histopathology observations. The most abundant active components available in our extract were gallic acid and the flavonoid quercetin. The F13b1/PV-EA has antiradical activity evidence by its inhibition of ABTS and DPPH free radicals. F13b1/PV-EA displayed against MCF-7 a suppressive effect with an IC50 value of 15.2 ± 1.35 µg/mL. Also, the expression of CAT, SOD, Caspase 3, Caspase 8 and Bax increased and the expression of Bcl-2 decreased. F13b1/PV-EA dose-dependently inhibited tumor development in cancer-induced mice. Thus, this finding introduces F13b1/PV-EA as an effectual apoptosis and antitumor active agent against breast cancer.
Maryam Seifaddinipour; Reyhaneh Farghadani; Farideh Namvar; Jamaludin Bin Mohamad; Nur Airina Muhamad. In Vitro and In Vivo Anticancer Activity of the Most Cytotoxic Fraction of Pistachio Hull Extract in Breast Cancer. Molecules 2020, 25, 1776 .
AMA StyleMaryam Seifaddinipour, Reyhaneh Farghadani, Farideh Namvar, Jamaludin Bin Mohamad, Nur Airina Muhamad. In Vitro and In Vivo Anticancer Activity of the Most Cytotoxic Fraction of Pistachio Hull Extract in Breast Cancer. Molecules. 2020; 25 (8):1776.
Chicago/Turabian StyleMaryam Seifaddinipour; Reyhaneh Farghadani; Farideh Namvar; Jamaludin Bin Mohamad; Nur Airina Muhamad. 2020. "In Vitro and In Vivo Anticancer Activity of the Most Cytotoxic Fraction of Pistachio Hull Extract in Breast Cancer." Molecules 25, no. 8: 1776.
A highly efficient protocol for regeneration of Carica papaya L. cv. Eksotika somatic embryos from immature zygotic embryos was developed. This study was designed to overcome the obstacles in regeneration of somatic embryos from immature zygotic embryos of “Eksotika”, especially problems associated with formation of better root quality and callus formation at the base of somatic embryos. Somatic embryos were generated by incubation of immature zygotic embryos in half-strength salt Murashige and Skoog (MS) medium with full-strength vitamins supplemented with 7.5 mg L−1 2,4-D, 100 mg L−1 L-glutamine, 50 mg L−1 myo-inositol, 45 mg L−1 adenine sulphate, 0.33% gelrite, and 6% sucrose, followed by transfer to maturation medium consisting of ½ MS medium supplemented with 5 mg L−1 phloroglucinol, 100 mg L−1 L-glutamine, 100 mg L−1 myo-inositol, 68 mg L−1 adenine sulphate, 0.38% gelrite, and 3% sucrose. After that, well-formed somatic embryos were transferred to MS medium containing 3% sucrose and 0.8% agar for shoot production. The embryos were elongated in MS medium supplemented with 1 mg L−1 gibberellic acid, 0.5 mg L−1 indole-3-butyric acid, 100 mg L−1 myo-inositol, and 3.76 mg L−1 riboflavin. Root regeneration was achieved on MS medium containing 7.9 mg L−1 phloroglucinol and supported with vermiculite after 4 days of cultivation on ½ MS medium with 2 mg L−1 indole-3-butyric acid. After the rooting phase, in vitro plantlets were acclimatized in peat moss soil.
Baker Al-Shara; Rosna Mat Taha; Jamaludin Mohamad; Hashimah Elias; Asif Khan. Somatic Embryogenesis and Plantlet Regeneration in the Carica papaya L. cv. Eksotika. Plants 2020, 9, 360 .
AMA StyleBaker Al-Shara, Rosna Mat Taha, Jamaludin Mohamad, Hashimah Elias, Asif Khan. Somatic Embryogenesis and Plantlet Regeneration in the Carica papaya L. cv. Eksotika. Plants. 2020; 9 (3):360.
Chicago/Turabian StyleBaker Al-Shara; Rosna Mat Taha; Jamaludin Mohamad; Hashimah Elias; Asif Khan. 2020. "Somatic Embryogenesis and Plantlet Regeneration in the Carica papaya L. cv. Eksotika." Plants 9, no. 3: 360.
Pueraria mirifica (PM) is a medicinal plant native to Thailand contained high amount of phytoestrogen and possesses anticancer activity. This study reports the effect of P. mirifica extract, phytoestrogen of diadzein and genistein for its benign prostate hyperplasia properties in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. The P. mirifica extract was evaluated for its total phenols, flavonoid and antioxidant activity using DPPH, FRAP and metal chelating assay. The assessment of P. mirifica, diadzein and genistein against benign prostate hyperplasia was determined in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. The total phenol was higher than flavonoid but showed low antioxidant activity of DPPH, FRAP and metal chelating. The aqueous PM extract at 1000 mg/kg significantly increased testosterone levels in testosterone-induced rats by 13% while diadzein and genistein increased it by 11% and 17% respectively. However, levels of FSH, LH, triglyceride and HDL are not affected by the oral administration of PM, diadzein and genistein to the rats. Similarly, total protein, albumin, globulin, total bilirubin, conjugated bilirubin, alkaline phosphatase, alanine aminotransferase, AST, and G-glutamyltransferase showed no significant difference as compared with negative control rats. The body weight of the rats, testis, kidney and liver showed no toxic effect. The zinc content increased significantly and the zinc transporter gen of ZnT4 and ZIP4 highly expressed suggesting that the PM, diadzein and genistein plays essential role in modulating prostate zinc homeostasis. Similarly, the expression of IL-6, AR and ER was significantly reduced indicating functioning in regulation of prostate growth and acts as anti-inflammatory role in preventing BPH. In conclusion, the results indicated that PM reduced BPH and contributed to the regulation in the zinc transport expression of the prostate cells in the benign prostate hyperplasia (BPH).
Jamaludin Mohamad; Siti Saleha Masrudin; Zazali Alias; Nur Airina Muhamad. The effects of Pueraria mirifica extract, diadzein and genistein in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. Molecular Biology Reports 2019, 46, 1855 -1871.
AMA StyleJamaludin Mohamad, Siti Saleha Masrudin, Zazali Alias, Nur Airina Muhamad. The effects of Pueraria mirifica extract, diadzein and genistein in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. Molecular Biology Reports. 2019; 46 (2):1855-1871.
Chicago/Turabian StyleJamaludin Mohamad; Siti Saleha Masrudin; Zazali Alias; Nur Airina Muhamad. 2019. "The effects of Pueraria mirifica extract, diadzein and genistein in testosterone-induced prostate hyperplasia in male Sprague Dawley rats." Molecular Biology Reports 46, no. 2: 1855-1871.
Haemorrhagic septicaemia (HS) is a well-known high fatality septicaemic disease happening among bovines. The disease is caused by the Pasteurella multocida serotype B:2 bacteria. P. multocida B:2 has high mortality and morbidity rates and is spread through the intranasal and oral routes in bovines. In this study, our aim was to investigate the efficacy of the recombinant protein vaccine, ABA392/pET30a via intranasal inoculation by targeting the mucosal immunity. The constructed recombinant protein vaccine ABA392/pET30a was subjected to an animal study using Sprague Dawley rats. The study was divided into two parts: active and passive immunization studies. Both studies were carried out through the determination of immunogenicity (using Total White Blood Cell (TWBC) Count with Indirect ELISA) and histopathogenicity, analyzing (Bronchus Associated Lymphoid Tissue (BALT) formation) in lungs. As a result, the IgA and IgG development of both tested groups: group 1 (50μg/mL protein vaccine) and group 2 (100μg/mL protein vaccine) showed equivalent with the positive control group 4 (formalin-killed P. multocida B:2). However, there was a significant difference when compared with the negative control group 3 (normal saline). These results demonstrate that both the protein vaccine at the concentration 50μg/mL and 100μg/mL have the same efficacy as the commercially available positive control vaccine. From the studies, higher concentration of protein vaccine at 100μg/mL showed higher development of both IgA and IgG compared to 50μg/mL protein vaccine. Higher and rapid development of IgA compared to IgG showed that mucosal immunity has been induced through the intranasal administration of the protein vaccine. In addition, leucocytosis was observed at each dose of vaccination showed that the protein vaccine is capable to induce the immune responses of the host. Histopathogenicity studies of the vaccinated groups showed more BALT formation and no severe lesions after challenge compared to the negative control group. Besides, no inflammatory onsite or anaphylactic responses were observed after the intranasal inoculation which proved to be safer and provided longer lasting immunity. Therefore, recombinant protein vaccine ABA392/pET30a could be a potential candidate for intranasal administration which can provoke mucosal immunity against HS disease.
Tzin Lim Kang; Rita Devi Velappan; Nurul Kabir; Jamaludin Mohamad; Nurshamimi Nor Rashid; Salmah Ismail; Kang Tzin Lim; Jamaluddin Mohamad. The ABA392/pET30a protein of Pasteurella multocida provoked mucosal immunity against HS disease in a rat model. Microbial Pathogenesis 2018, 128, 90 -96.
AMA StyleTzin Lim Kang, Rita Devi Velappan, Nurul Kabir, Jamaludin Mohamad, Nurshamimi Nor Rashid, Salmah Ismail, Kang Tzin Lim, Jamaluddin Mohamad. The ABA392/pET30a protein of Pasteurella multocida provoked mucosal immunity against HS disease in a rat model. Microbial Pathogenesis. 2018; 128 ():90-96.
Chicago/Turabian StyleTzin Lim Kang; Rita Devi Velappan; Nurul Kabir; Jamaludin Mohamad; Nurshamimi Nor Rashid; Salmah Ismail; Kang Tzin Lim; Jamaluddin Mohamad. 2018. "The ABA392/pET30a protein of Pasteurella multocida provoked mucosal immunity against HS disease in a rat model." Microbial Pathogenesis 128, no. : 90-96.
Ethnopharmacological importance Leptospermum flavescens has been used traditionally in Malaysia to treat various ailments such as constipation, hypertension, diabetes and cancer. To investigate the potential protective effects of L. flavescens in pancreatic β cells through inhibition of apoptosis and autophagy cell death mechanisms in in vitro and in vivo models. L. flavescens leaves were extracted using solvent in increasing polarities: hexane, ethyl acetate, methanol and water. All extracts were tested for INS-1 β cells viability stimulated by streptozotocin (STZ). The extract which promotes the highest cell protective activity was further evaluated for insulin secretion, apoptosis and autophagy signaling pathways. Then, the acute toxicity of extract was carried out in SD rats according to OECD 423 guideline. The active extract was tested in diabetic rats where the pancreatic β islets were evaluated for insulin, apoptosis and autophagy protein. The methanolic extract of L. flavescens (MELF) was found to increase INS-1 β cells viability and insulin secretion against STZ. In addition, MELF has been shown to inhibit INS-1 β cells apoptosis and autophagy activity. Notably, there was no toxicity observed in SD rats when administered with MELF. Furthermore, MELF exhibited anti-hyperglycemic activity in diabetic rats where apoptosis and autophagy protein expression was found to be suppressed in pancreatic β islets. MELF was found to protect pancreatic β cells function from STZ-induced apoptosis and autophagy in in vitro and in vivo.
Ahmad Fadhlurrahman Ahmad Hidayat; Chim Kei Chan; Jamaludin Mohamad; Habsah Abdul Kadir. Leptospermum flavescens Sm. protect pancreatic β cell function from streptozotocin involving apoptosis and autophagy signaling pathway in in vitro and in vivo case study. Journal of Ethnopharmacology 2018, 226, 120 -131.
AMA StyleAhmad Fadhlurrahman Ahmad Hidayat, Chim Kei Chan, Jamaludin Mohamad, Habsah Abdul Kadir. Leptospermum flavescens Sm. protect pancreatic β cell function from streptozotocin involving apoptosis and autophagy signaling pathway in in vitro and in vivo case study. Journal of Ethnopharmacology. 2018; 226 ():120-131.
Chicago/Turabian StyleAhmad Fadhlurrahman Ahmad Hidayat; Chim Kei Chan; Jamaludin Mohamad; Habsah Abdul Kadir. 2018. "Leptospermum flavescens Sm. protect pancreatic β cell function from streptozotocin involving apoptosis and autophagy signaling pathway in in vitro and in vivo case study." Journal of Ethnopharmacology 226, no. : 120-131.
Plumeria rubra Linn of the family Apocynaceae is locally known in Malaysia as “Kemboja”. It has been used by local traditional medicine practitioners for the treatment of arthritis-related disease. The LCMS/MS analysis of the methanol extract of flowers (PR-ME) showed that it contains 3-O-caffeyolquinic acid, 5-caffeoquinic acid, 1,3-dicaffeoquinic acid, chlorogenic acid, citric acid, 3,3-di-O-methylellagic acid, kaempferol-3-O-glucoside, kaempferol-3-rutinoside, kaempferol, quercetin 3-O-α-l-arabinopyranoside, quercetin, quinic acid and rutin. The flower PR-ME contained high amounts of phenol and flavonoid at 184.632 mg GAE/g and 203.2.2 mg QE/g, respectively. It also exhibited the highest DPPH, FRAP, metal chelating, hydrogen peroxide, nitric oxide superoxide radical scavenging activity. Similarly, the XO inhibitory activity in vitro assay possesses the highest inhibition effects at an IC50 = 23.91 μg/mL. There was no mortality or signs of toxicity in rats at a dose of 4 g/kg body weight. The administration of the flower PR-ME at doses of 400 mg/kg to the rats significantly reduced serum uric acid 43.77%. Similarly, the XO activity in the liver was significantly inhibited by flower PR-ME at doses of 400 mg/kg. These results confirm that the flower PR-ME of P. rubra contains active phytochemical compounds as detected in LCMS/MS that contribute to the inhibition of XO activity in vitro and in vivo in reducing acid uric level in serum and simultaneously scavenging the free radical to reduce the oxidative stress.
Siti Sarwani Putri Mohamed Isa; Abdulwali Ablat; Jamaludin Mohamad. The Antioxidant and Xanthine Oxidase Inhibitory Activity of Plumeria rubra Flowers. Molecules 2018, 23, 400 .
AMA StyleSiti Sarwani Putri Mohamed Isa, Abdulwali Ablat, Jamaludin Mohamad. The Antioxidant and Xanthine Oxidase Inhibitory Activity of Plumeria rubra Flowers. Molecules. 2018; 23 (2):400.
Chicago/Turabian StyleSiti Sarwani Putri Mohamed Isa; Abdulwali Ablat; Jamaludin Mohamad. 2018. "The Antioxidant and Xanthine Oxidase Inhibitory Activity of Plumeria rubra Flowers." Molecules 23, no. 2: 400.
Xanthine oxidase (XO) plays an important role in the regulation of uric acid and prevents it from being overproduced as in hyperuricemia disease. The combined effects of antioxidant and xanthine oxidase inhibitor would become a promising approach for hyperuricemia treatment. In this research, the antioxidant and xanthine oxidase inhibitory activities of Pandanus amaryllifolius leaf were evaluated. The leaf water extract (PA-W) showed highest total phenols, and petroleum ether extract (PA-PE) showed highest total flavonoids contents. The antioxidant activity of DPPH, metal chelating and hydrogen peroxide was highest in PA-W extract. The treatment of PA-W extract at 1000 mg kg−1 body weight in potassium oxonate-induced hyperuricemic rats showed significant (P < 0.001) decrease in serum uric acid level by 85% and XO activity by 64%, respectively, as compared to the hyperuricemic rats. In conclusion, the P. amaryllifolius possess the dual effect of antioxidant and XO inhibition as potential therapeutic agents in the hyperuricemia treatment.
Nur Afira Ahmad Shukor; Abdulwali Ablat; Nur Airina Muhamad; Jamaludin Mohamad. In vitro antioxidant and in vivo xanthine oxidase inhibitory activities of Pandanus amaryllifolius in potassium oxonate-induced hyperuricemic rats. International Journal of Food Science & Technology 2018, 53, 1476 -1485.
AMA StyleNur Afira Ahmad Shukor, Abdulwali Ablat, Nur Airina Muhamad, Jamaludin Mohamad. In vitro antioxidant and in vivo xanthine oxidase inhibitory activities of Pandanus amaryllifolius in potassium oxonate-induced hyperuricemic rats. International Journal of Food Science & Technology. 2018; 53 (6):1476-1485.
Chicago/Turabian StyleNur Afira Ahmad Shukor; Abdulwali Ablat; Nur Airina Muhamad; Jamaludin Mohamad. 2018. "In vitro antioxidant and in vivo xanthine oxidase inhibitory activities of Pandanus amaryllifolius in potassium oxonate-induced hyperuricemic rats." International Journal of Food Science & Technology 53, no. 6: 1476-1485.
Pistachio (Pistacia vera L.) hulls (PVLH) represents a significant by-product of industrial pistachio processing that contains high amounta of phenolic and flavonoid compounds known to act as antioxidants. The current study was designed to evaluate the anti-tumor and anti-angiogenic potentials of PVLH extracts. The cytotoxic effects of hexane, ethyl acetate, methanol, and water PVLH extracts toward human colon cancer (HT-29 and HCT-116), breast adenocarcinoma (MCF-7), lung adenocarcinoma (H23), liver hepatocellular carcinoma (HepG2), cervical cancer (Ca Ski), and normal fibroblast (BJ-5ta) cells were assessed using a MTT cell viability assay. Apoptosis induction was evaluated through the different nuclear staining assays and confirmed by flow cytometry analysis. Anti-angiogenic activities were also determined using chorioallantoic membrane (CAM) assay. PVLH ethyl acetate extracts (PVLH-EAE) demonstrated a suppressive effect with an IC50 value of 21.20 ± 1.35, 23.00 ± 1.2 and 25.15 ± 1.85 µg/mL against MCF-7, HT-29 and HCT-116, respectively, after 72 h of treatment. Morphological assessment and flow cytometry analysis showed the potential of PVLH-EAE to induce apoptosis. PVLH-EAE at the highest concentration demonstrated significant inhibition of angiogenesis as comparing with control group. Also the expression of Bax increased and the expression of Bcl-2 decreased in treated MCF-7 cells. Thus, the apoptosis induction and angiogenesis potential of PVLH-EAE make it to be the most suitable for further cancer research study to deal with selective antitumor active substances to human cancers especially breast cancer.
Maryam Seifaddinipour; Reyhaneh Farghadani; Farideh Namvar; Jamaludin Mohamad; Habsah Abdul Kadir. Cytotoxic Effects and Anti-Angiogenesis Potential of Pistachio (Pistacia vera L.) Hulls against MCF-7 Human Breast Cancer Cells. Molecules 2018, 23, 110 .
AMA StyleMaryam Seifaddinipour, Reyhaneh Farghadani, Farideh Namvar, Jamaludin Mohamad, Habsah Abdul Kadir. Cytotoxic Effects and Anti-Angiogenesis Potential of Pistachio (Pistacia vera L.) Hulls against MCF-7 Human Breast Cancer Cells. Molecules. 2018; 23 (1):110.
Chicago/Turabian StyleMaryam Seifaddinipour; Reyhaneh Farghadani; Farideh Namvar; Jamaludin Mohamad; Habsah Abdul Kadir. 2018. "Cytotoxic Effects and Anti-Angiogenesis Potential of Pistachio (Pistacia vera L.) Hulls against MCF-7 Human Breast Cancer Cells." Molecules 23, no. 1: 110.
Wan Nurul Nazneem Wan Othman; Yasodha Sivasothy; Sook Yee Liew; Jamaludin Mohamad; Mohd Azlan Nafiah; Kartini Ahmad; Marc Litaudon; Khalijah Awang. Alkaloids from Cryptocarya densiflora Blume (Lauraceae) and their cholinesterase inhibitory activity. Phytochemistry Letters 2017, 21, 230 -236.
AMA StyleWan Nurul Nazneem Wan Othman, Yasodha Sivasothy, Sook Yee Liew, Jamaludin Mohamad, Mohd Azlan Nafiah, Kartini Ahmad, Marc Litaudon, Khalijah Awang. Alkaloids from Cryptocarya densiflora Blume (Lauraceae) and their cholinesterase inhibitory activity. Phytochemistry Letters. 2017; 21 ():230-236.
Chicago/Turabian StyleWan Nurul Nazneem Wan Othman; Yasodha Sivasothy; Sook Yee Liew; Jamaludin Mohamad; Mohd Azlan Nafiah; Kartini Ahmad; Marc Litaudon; Khalijah Awang. 2017. "Alkaloids from Cryptocarya densiflora Blume (Lauraceae) and their cholinesterase inhibitory activity." Phytochemistry Letters 21, no. : 230-236.
Brucea javanica (B. javanica) seeds, also known as "Melada pahit" in Indo-Malay region are traditionally used to treat diabetes. The objective of this study was to determine antidiabetic, antioxidant and anti-inflammatory effects of B. javanica seeds on nicotinamide (NA)-streptozotocin (STZ) induced type 2 diabetic (T2D) rats and to analyze its chemical composition that correlate with their pharmacological activities. A hydroethanolic extract of B. javanica seeds was fractionated with n-hexane, chloroform and ethyl acetate. An active fraction was selected after screening for its ability to inhibit α-glucosidase and glycogen phosphorylase α (GP-α). Isolation and characterization were carried out by using column chromatography, NMR and LCMS/MS. All isolates were assayed for inhibition of GP-α and α-glucosidase. Antidiabetic effect of active fraction was further evaluated in T2D rat model. Blood glucose and body weight were measured weekly. Serum insulin, lipid profile, renal function, liver glycogen and biomarkers of oxidative stress and inflammation were analyzed after 4-week treatment and compared with standard drug glibenclamide. Ethyl acetate fraction (EAF) exerted good inhibitory potential for α-glucosidase and GP-α compared with other fractions. Chromatographic isolation of the EAF led to the identification of seven compounds: vanillic acid (1), bruceine D (2), bruceine E (3), parahydroxybenzoic acid (4), luteolin (5), protocatechuic acid (6), and gallic acid (7). Among them, Compound (5) was identified as the most potent inhibitor of GP-α and α-glucosidase and its GP-α inhibitory activity (IC50 = 45.08 μM) was 10-fold higher than that of caffeine (IC50 = 457.34 μM), and α-glucosidase inhibitory activity (IC50 = 26.41 μM) was 5.5-fold higher than that of acarbose (IC50 = 145.83 μM), respectively. Compounds (4), (6), and (7) inhibited GP-α activity in a concentration-dependent manner with IC50 values of 357.88, 297.37, and 214.38 μM, and their inhibitory effect was higher than that of caffeine. These compounds exhibited weak potency on α-glucosidase compared with acarbose. Compounds (1), (2), and (3) showed no inhibition on both GP-α and α-glucosidase. In vivo study showed that EAF treatment significantly reduced blood glucose level, increased insulin and glycogen contents, decreased markers of oxidative stress and inflammation, and lipid levels in T2D rats compared with untreated group. The EAF has potential therapeutic value for the treatment of T2D via acting as GP-α and α-glucosidase inhibitors by improving hepatic glucose and carbohydrate metabolism, suppressing oxidative stress, and preventing inflammation in T2D rats. According to the results, the efficacy of EAF could be due to the presence of luteolin along with synergistic effect of multiple compounds such as parahydroxybenzoic acid, protocatechuic acid, and gallic acid in B. javanica seeds.
Abdulwali Ablat; Mohammed Halabi; Jamaludin Mohamad; Muhammad Hafiz Husna Hasnan; Hazrina Hazni; Ser-Huy Teh; Jamil Shilpi; Zulqarnain Mohamed; Khalijah Awang. Antidiabetic effects of Brucea javanica seeds in type 2 diabetic rats. BMC Complementary and Alternative Medicine 2017, 17, 94 .
AMA StyleAbdulwali Ablat, Mohammed Halabi, Jamaludin Mohamad, Muhammad Hafiz Husna Hasnan, Hazrina Hazni, Ser-Huy Teh, Jamil Shilpi, Zulqarnain Mohamed, Khalijah Awang. Antidiabetic effects of Brucea javanica seeds in type 2 diabetic rats. BMC Complementary and Alternative Medicine. 2017; 17 (1):94.
Chicago/Turabian StyleAbdulwali Ablat; Mohammed Halabi; Jamaludin Mohamad; Muhammad Hafiz Husna Hasnan; Hazrina Hazni; Ser-Huy Teh; Jamil Shilpi; Zulqarnain Mohamed; Khalijah Awang. 2017. "Antidiabetic effects of Brucea javanica seeds in type 2 diabetic rats." BMC Complementary and Alternative Medicine 17, no. 1: 94.
A new acylphenol, malabaricone E (1) together with the known malabaricones A-C (2-4), maingayones A and B (5 and 6) and maingayic acid B (7) were isolated from the ethyl acetate extract of the fruits of Myristica cinnamomea King. Their structures were determined by 1D and 2D NMR techniques and LCMS-IT-TOF analysis. Compounds 3 (1.84 ± 0.19 and 1.76 ± 0.21 μM, respectively) and 4 (1.94 ± 0.27 and 2.80 ± 0.49 μM, respectively) were identified as dual inhibitors, with almost equal acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes inhibiting potentials. The Lineweaver-Burk plots of compounds 3 and 4 indicated that they were mixed-mode inhibitors. Based on the molecular docking studies, compounds 3 and 4 interacted with the peripheral anionic site (PAS), the catalytic triad and the oxyanion hole of the AChE. As for the BChE, while compound 3 interacted with the PAS, the catalytic triad and the oxyanion hole, compound 4 only interacted with the catalytic triad and the oxyanion hole.
Siti Mariam Abdul Wahab; Yasodha Sivasothy; Sook Yee Liew; Marc Litaudon; Jamaludin Mohamad; Khalijah Awang. Natural cholinesterase inhibitors from Myristica cinnamomea King. Bioorganic & Medicinal Chemistry Letters 2016, 26, 3785 -3792.
AMA StyleSiti Mariam Abdul Wahab, Yasodha Sivasothy, Sook Yee Liew, Marc Litaudon, Jamaludin Mohamad, Khalijah Awang. Natural cholinesterase inhibitors from Myristica cinnamomea King. Bioorganic & Medicinal Chemistry Letters. 2016; 26 (15):3785-3792.
Chicago/Turabian StyleSiti Mariam Abdul Wahab; Yasodha Sivasothy; Sook Yee Liew; Marc Litaudon; Jamaludin Mohamad; Khalijah Awang. 2016. "Natural cholinesterase inhibitors from Myristica cinnamomea King." Bioorganic & Medicinal Chemistry Letters 26, no. 15: 3785-3792.
Giganteone E (1), a new dimeric acylphenol was isolated as a minor constituent from the bark of Myristica maxima Warb. The structure of 1 was established on the basis of 1D and 2D NMR techniques and LCMS-IT-TOF analysis. Malabaricones A–C (2–4), giganteones A and C (5 and 6), maingayones A and B (7 and 8), maingayic acid B (9) and β-sitosteryl oleate (10) were also characterized in this plant for the first time. Compound 10 was identified for the first time in the Myristicaceae. Compounds 2 and 5 were active against human prostate cancer cell-lines, thus making this the first report on the prostate cancer inhibiting potential of acylphenols and dimeric acylphenols. Compounds 1, 4, 5, 7 and 8 exhibited potent DPPH free radical scavenging activity. This is the first report on their free radical scavenging capacity.
Muhamad Aqmal Othman; Yasodha Sivasothy; Chung Yeng Looi; Abdulwali Ablat; Jamaludin Mohamad; Marc Litaudon; Khalijah Awang. Acylphenols and dimeric acylphenols from Myristica maxima Warb. Fitoterapia 2016, 111, 12 -17.
AMA StyleMuhamad Aqmal Othman, Yasodha Sivasothy, Chung Yeng Looi, Abdulwali Ablat, Jamaludin Mohamad, Marc Litaudon, Khalijah Awang. Acylphenols and dimeric acylphenols from Myristica maxima Warb. Fitoterapia. 2016; 111 ():12-17.
Chicago/Turabian StyleMuhamad Aqmal Othman; Yasodha Sivasothy; Chung Yeng Looi; Abdulwali Ablat; Jamaludin Mohamad; Marc Litaudon; Khalijah Awang. 2016. "Acylphenols and dimeric acylphenols from Myristica maxima Warb." Fitoterapia 111, no. : 12-17.
To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromethane extract of Alseodaphne corneri.Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography. The antiplasmodial activity of the isolated compounds was evaluated using the histidine-rich protein II assay. The isolated alkaloids were also tested for their antioxidant activity using three different assays; DPPH, ferric reducing ability of plasma and metal chelating assays.Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoptosis. The antioxidant properties of the alkaloids under investigation revealed that in addition to the antiplasmodial activity, the alkaloids could also prevent oxidative stress. (+)-laurotetanine and (+)-norstephasubine exhibited strong antiplasmodial activities with IC50 values of 0.189 and 0.116 μM, respectively.Interestingly, the two most potent compounds that exhibit antiplasmodial activity also exhibit good antioxidant activities. The crude dichloromethane extract and the isolated compounds exert substantial antiplasmodial and antioxidative activities which in turn suppress oxidative stress and cause less damage to the host.
Azeana Zahari; Abdulwali Ablat; Yasodha Sivasothy; Jamaludin Mohamad; M. Iqbal Choudhary; Khalijah Awang. In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm. Asian Pacific Journal of Tropical Medicine 2016, 9, 328 -332.
AMA StyleAzeana Zahari, Abdulwali Ablat, Yasodha Sivasothy, Jamaludin Mohamad, M. Iqbal Choudhary, Khalijah Awang. In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm. Asian Pacific Journal of Tropical Medicine. 2016; 9 (4):328-332.
Chicago/Turabian StyleAzeana Zahari; Abdulwali Ablat; Yasodha Sivasothy; Jamaludin Mohamad; M. Iqbal Choudhary; Khalijah Awang. 2016. "In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm." Asian Pacific Journal of Tropical Medicine 9, no. 4: 328-332.
The crude extract of the bark of Dehaasia longipedicellata exhibited antiplasmodial activity against the growth of Plasmodium falciparum K1 isolate (resistant strain). Phytochemical studies of the extract led to the isolation of six alkaloids: two morphinandienones, (+)-sebiferine (1) and (−)-milonine (2); two aporphines, (−)-boldine (3) and (−)-norboldine (4); one benzlyisoquinoline, (−)-reticuline (5); and one bisbenzylisoquinoline, (−)-O-O-dimethylgrisabine (6). Their structures were determined on the basis of 1D and 2D NMR, IR, UV, and LCMS spectroscopic techniques and upon comparison with literature values. Antiplasmodial activity was determined for all of the isolated compounds. They showed potent to moderate activity with IC50 values ranging from 0.031 to 30.40 µM. (−)-O-O-dimethylgrisabine (6) and (−)-milonine (2) were the two most potent compounds, with IC50 values of 0.031 and 0.097 µM, respectively, that were comparable to the standard, chloroquine (0.090 µM). The compounds were also assessed for their antioxidant activities with di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (IC50 = 18.40–107.31 µg/mL), reducing power (27.40–87.40 %), and metal chelating (IC50 = 64.30 to 257.22 µg/mL) having good to low activity. (−)-O-O-dimethylgrisabine (6) exhibited a potent antioxidant activity of 44.3 % reducing power, while di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium and metal chelating activities had IC50 values of 18.38 and 64.30 µg/mL, respectively. Thus it may be considered as a good reductant with the ability to chelate metal and prevent pro-oxidant activity. In addition to the antiplasmodial and antioxidant activities, the isolated compounds were also tested for their cytotoxicity against a few cancer and normal cell lines. (−)-Norboldine (4) exhibited potent cytotoxicity towards pancreatic cancer cell line BxPC-3 with an IC50 value of 27.060 ± 1.037 µM, and all alkaloids showed no toxicity towards the normal pancreatic cell line (hTERT-HPNE).
Azeana Zahari; Foo Kit Cheah; Jamaludin Mohamad; Syazreen Nadia Sulaiman; Marc Litaudon; Kok Hoong Leong; Khalijah Awang. Antiplasmodial and Antioxidant Isoquinoline Alkaloids from Dehaasia longipedicellata. Planta Medica 2014, 80, 599 -603.
AMA StyleAzeana Zahari, Foo Kit Cheah, Jamaludin Mohamad, Syazreen Nadia Sulaiman, Marc Litaudon, Kok Hoong Leong, Khalijah Awang. Antiplasmodial and Antioxidant Isoquinoline Alkaloids from Dehaasia longipedicellata. Planta Medica. 2014; 80 (07):599-603.
Chicago/Turabian StyleAzeana Zahari; Foo Kit Cheah; Jamaludin Mohamad; Syazreen Nadia Sulaiman; Marc Litaudon; Kok Hoong Leong; Khalijah Awang. 2014. "Antiplasmodial and Antioxidant Isoquinoline Alkaloids from Dehaasia longipedicellata." Planta Medica 80, no. 07: 599-603.
A dichloromethane extract of the stem bark of Cryptocarya nigra showed strong in vitro inhibition of Plasmodium falciparum growth, with an IC50 value of 2.82 μg/mL. The phytochemical study of this extract has led to the isolation and characterization of four known alkaloids: (+)-N-methylisococlaurine (1), atherosperminine (2), 2-hydroxyathersperminine (3), and noratherosperminine (4). Structural elucidation of all alkaloids was accomplished by means of high field 1D- and 2D-NMR, IR, UV and LCMS spectral data. The isolated extract constituents (+)-N-methylisococlaurine (1), atherosperminine (2) and 2-hydroxy-atherosperminine (3) showed strong antiplasmodial activity, with IC50 values of 5.40, 5.80 and 0.75 μM, respectively. In addition, (+)-N-methylisocolaurine (1) and atherosperminine (2) showed high antioxidant activity in a DPPH assay with IC50 values of 29.56 ug/mL and 54.53 ug/mL respectively. Compounds 1 and 2 also both showed high antioxidant activity in the FRAP assay, with percentages of 78.54 and 70.66 respectively and in the metal chelating assay, with IC50 values of 50.08 ug/mL and 42.87 ug/mL, respectively.
Ayu Afiqah Nasrullah; Azeana Zahari; Jamaludin Mohamad; Khalijah Awang. Antiplasmodial Alkaloids from the Bark of Cryptocarya nigra (Lauraceae). Molecules 2013, 18, 8009 -8017.
AMA StyleAyu Afiqah Nasrullah, Azeana Zahari, Jamaludin Mohamad, Khalijah Awang. Antiplasmodial Alkaloids from the Bark of Cryptocarya nigra (Lauraceae). Molecules. 2013; 18 (7):8009-8017.
Chicago/Turabian StyleAyu Afiqah Nasrullah; Azeana Zahari; Jamaludin Mohamad; Khalijah Awang. 2013. "Antiplasmodial Alkaloids from the Bark of Cryptocarya nigra (Lauraceae)." Molecules 18, no. 7: 8009-8017.
Eleven compounds:goniomicin A (1), goniomicin B (2), goniomicin C (3), goniomicin D (4), tapisoidin (5), goniothalamin (6), 9-deoxygoniopypyrone (7), pterodondiol (8), liriodenine (9), benzamide (10) and cinnamic acid (11), were isolated from the stem bark of Goniothalamus tapisoides. All compounds were identified by spectroscopic analysis and, for known compounds, by comparison with published data. Goniothalamin (6) exhibited mild cytotoxic activity towards a colon cancer cell line (HT-29), with an IC50value of 64.17 ± 5.60 µM. Goniomicin B (2) give the highest antioxidant activity in the DPPH assay among all compounds tested, with an IC50 of 0.207 µM.
Rosalind Pei Theng Kim; Vicky Bihud; Khalit Bin Mohamad; Kok Hoong Leong; Jamaludin Bin Mohamad; Fasihuddin Bin Ahmad; Hazrina Hazni; Noraini Kasim; Siti Nadiah Abdul Halim; Khalijah Awang. Cytotoxic and Antioxidant Compoundsfrom the Stem Bark of Goniothalamus tapisoides Mat Salleh. Molecules 2012, 18, 128 -139.
AMA StyleRosalind Pei Theng Kim, Vicky Bihud, Khalit Bin Mohamad, Kok Hoong Leong, Jamaludin Bin Mohamad, Fasihuddin Bin Ahmad, Hazrina Hazni, Noraini Kasim, Siti Nadiah Abdul Halim, Khalijah Awang. Cytotoxic and Antioxidant Compoundsfrom the Stem Bark of Goniothalamus tapisoides Mat Salleh. Molecules. 2012; 18 (1):128-139.
Chicago/Turabian StyleRosalind Pei Theng Kim; Vicky Bihud; Khalit Bin Mohamad; Kok Hoong Leong; Jamaludin Bin Mohamad; Fasihuddin Bin Ahmad; Hazrina Hazni; Noraini Kasim; Siti Nadiah Abdul Halim; Khalijah Awang. 2012. "Cytotoxic and Antioxidant Compoundsfrom the Stem Bark of Goniothalamus tapisoides Mat Salleh." Molecules 18, no. 1: 128-139.
A new neolignan, 3,4-dimethoxy-3′,4′-methylenedioxy-2,9-epoxy-6,7-cyclo-1,8-neolign-11-en-5(5H)-one, which has been named (+)-kunstlerone (1), together with six known alkaloids: (+)-norboldine (2), (+)-N-methylisococlaurine (3), (+)-cassythicine (4), (+)-laurotetanine (5), (+)-boldine (6) and (-)-pallidine (7), were isolated from the leaves of Beilschmiedia kunstleri. The structures were established through various spectroscopic methods notably 1D- and 2D-NMR, UV, IR and LCMS-IT-TOF. (+)- Kunstlerone (1) showed a strong antioxidant activity, with an SC50 of 20.0 µg/mL.
Abbas Mollataghi; A Hamid A Hadi; Khalijah Awang; Jamaludin Mohamad; Marc Litaudon; Mat Ropi Mukhtar. (+)-Kunstlerone, a New Antioxidant Neolignan from the Leaves of Beilschmiedia kunstleri Gamble. Molecules 2011, 16, 6582 -6590.
AMA StyleAbbas Mollataghi, A Hamid A Hadi, Khalijah Awang, Jamaludin Mohamad, Marc Litaudon, Mat Ropi Mukhtar. (+)-Kunstlerone, a New Antioxidant Neolignan from the Leaves of Beilschmiedia kunstleri Gamble. Molecules. 2011; 16 (8):6582-6590.
Chicago/Turabian StyleAbbas Mollataghi; A Hamid A Hadi; Khalijah Awang; Jamaludin Mohamad; Marc Litaudon; Mat Ropi Mukhtar. 2011. "(+)-Kunstlerone, a New Antioxidant Neolignan from the Leaves of Beilschmiedia kunstleri Gamble." Molecules 16, no. 8: 6582-6590.