The aim of this study was to determine the prevalence of soil transmitted helminthes among primary school children. School based cross-sectional study design was employed. A total of six hundred study subjects were selected by a multistage sampling method. Fresh stool specimens were collected using clean, dry and wide mouthed labeled stool cups. It was processed by Kato-Katz technique. The data were analyzed using SPSS version 20 and p-value < 0.05 was considered statistically significant. The overall prevalence of soil transmitted helminthes was 57 (9.5%). Hookworm was the most prevalent helminthes species isolated (4.2%) followed by A. lumbricoide (3%). The prevalence of Taenia species, T. trichiura, H. nana and E. vermicularis were; 1.2%, 0.5%, 0.7% and 0.8% respectively. The prevalence of the Soil transmitted helminthes infection was low and all cases of Soil transmitted infections in this study were with low infection intensity. This might be due to the preventive chemotherapy given to the school children.

Introduction: Laboratory services are crucial parts of the health system having a great contribution to disease prevention and management. The importance of accurate and reliable laboratory test results is less recognized in developing countries like Ethiopia where most medical decisions are based on clinical judgment. It is time for countries like Ethiopia to not only increase health care coverage but also improve access to essential diagnostic tests. Hence, this proposed study aims to assess essential in-vitro laboratory service provision in accordance with the WHO standards in Guragae Zone primary health care unit level, South Ethiopia.Methods: Health institution-based cross-sectional study was carried out. 30% randomly selected primary health care units were recruited. Each facility was visited with a WHO checklist by a trained data collector to assess the availability of essential diagnostics service provision. The proportion of available in-vitro diagnostics services was calculated. Results were presented as percentages in tables and figures.Result: Twenty-one primary health care facilities located in Guragae Zone were assessed between May and July 2019. All surveyed facilities had major gaps in essential test availability. Among essential diagnostic tests listed with WHO like C-reactive protein, lipid profile, Amylase and Lipase, TroponinT/I, hepatitis B e-antigen, IgM-specific antibodies to hepatitis B core antigen, Glucose-6-phosphate dehydrogenase activity, and anti-HIV/p24 rapid test were not provided in any facilities. However, essential diagnostic services like urine dipstick testing, random blood sugar, smear microscopy, and few serological tests were provided at all primary health care units. All surveyed facilities had limited major laboratory equipment and consumables.Conclusion and recommendation: The present study shows limited access to essential laboratory tests at the primary health care level. Hence, the responsible body should invest to make essential tests accessible at the primary care unit level within the framework of universal health coverage in the study area. The fact that access to essential diagnostic tests is the first key step in improving quality of care; such study has its own efforts to enable the implementation of essential diagnostic lists, and improve access to diagnostics in the country.

Background and aim: School-based preventive mass chemotherapy has been a key component of Ethiopia's national plan for the control of soil-transmitted helminths. Without an impact evaluation on the impact of a deworming program on infection levels, it is unclear whether the deworming program warrants levels of environmental transmission of infection. This study aimed to determine the impact of annual preventive mass chemotherapy for soil-transmitted helminths among schoolchildren in an endemic area of Gurage zone, south-central Ethiopia. Methods: A repeated school-based quantitative prospective cross-sectional method was employed. Data were collected from study participants selected using systematic sampling with probability proportional to size at baseline and after annual treatment. Fresh stool samples were collected and processed using the Kato-Katz technique at the Wolkite University parasitology laboratory. SPSS-21 was used for data management and analysis. Changes in parasitological variables after treatment were estimated. Results: Overall, 41.1% prevalence and 22.3% mean geometric infection-intensity reduction were found. Reductions in prevalence of Schistosoma mansoni and hookworms were 13.2% and 15.3%, respectively. Similarly, decreases in prevalence were seen in Ascaris lumbricoides and Trichuris trichiura, representing 94.4% and 80.0% reduction rates, respectively, while 25.9% of the children had heavy S. mansoni (>= 400 eggs per gram) infections at baseline, which were reduced to 4.5% after annual treatment. Geometric mean infection intensity-reduction rates for hookworms, A. lumbricoides, and T. trichiura were 80.8%, 20.2%, and 96.7%, respectively. Conclusion: Annual mass chemotherapy failed to clear soil-transmitted helminths completely in the present study. However, it resulted in a substantial reduction in overall prevalence and infection intensity. Therefore, other than deworming for school children, interventions such as access to improved personal hygiene and environmental hygiene in school should be emphasized to interrupt transmission.

Malaria continues to be a public health problem and important cause of morbidity and mortality in Ethiopia. Due to continuous interventions to combat malaria in endemic regions, a decline in malaria related deaths and morbidity has been registered. These gains, however, are threatened with the emergency of antimalarial drugs resistant strains of plasmodium parasites. This study aimed to determine therapeutic efficacy of chloroquine for treatment of Plasmodium vivax malaria cases in Guragae zone, southern central Ethiopia. A one arm prospective study with recurrence of parasitaemia and clinical conditions of patients were evaluated on days 0, 1, 2, 3, 7, 14, and 28. Patients with Plasmodium vivax malaria mono infection and eligible for study inclusion criteria were recruited. SPSS-21 used for data analysis and management. Kaplan-Meier survival probability analysis was estimated. Mean geometric parasitaemia and average haemoglobin concentration were calculated. Among 87 total recruited subjects, 81 of them completed the 28 days follow up. More than half of (57.5%) the study participants had a history of fever and 42.5% of them had fever at the time of enrollment. The mean body temperature on day of recruitment was 38.2 °C and 36.8 °C on day 28. Geometric mean parasitaemia calculated on day of enrollment was 2270 parasites/μl of blood. Recurrence of parasitaemia was registered from two subjects during entire follow up. The mean haemoglobin concentration of study participants on day of enrolment was 11.8 g/ dl and 13.8 g/dl on day 28. This study registered a high chloroquine efficacy rate among the study participants. Therefore, chloroquine remains efficacious for the treatment of Plasmodium vivax malaria in the study area. However, there is a need to monitor chloroquine resistance by employing molecular tools for better evaluation of treatment outcome.

Helminth infections are among the major public health problems in developing countries. Considerable efforts have been made towards the control of morbidity caused by infection with helminths in Ethiopia. The national control program is designed to achieve the elimination of helminth infections as a major public health problem by 2020. The objective of this study was to determine the current status and infection intensity of helminths in the endemic area of Guragae zone, Southern Ethiopia. An institutional based cross-sectional study was carried out between April and June 2017 in Gurage zone. School-aged children (SAC) were selected using a multistage sampling method and invited to participate in the study. Parasitological test examination was done using the Kato-Katz technique in Wolkite University parasitology laboratory. SPSS version 21 was used for data management and analysis. A total of 597 (98% compliance rate) participants were able to provide complete data. The study revealed that 21.6% (129/597) SAC were infected with one or more species of helminth. S. mansoni was the most prevalent helminth (12.9%) followed by hookworms (4.3%). The overall infection intensity expressed as geometric mean for A. lumbricoides, T. trichiura, hookworms, and S. mansoni were 301, 31,103, and 158 eggs per gram of stool, respectively. The multivariable logistic regression model estimated that being in the age group of 5–9 years (AOR = 1.43, 95% CI 0.4–0.9), washing raw food and vegetables using river water (AOR = 2.4, 95% CI 0.16–0.75), and a regular bathing habit in river (AOR = 2.14, 95% CI 0.3–0.9) were independent predictors of helminth infections. Despite the fact that Ethiopia planned to eliminate helminth infection-related morbidity by 2020, this study showed that helminth infection is prevalent in the study area. Efforts should be made to improve hygienic practices of the schoolchildren in addition to school-based deworming. Moreover, the deworming program should also focus on reaching those SAC who do not attend school through communal social places to achieve the targeted goal in the study area in particular and nationwide in general.

Background: Accurate early diagnosis and prompt treatment is one of the core strategies employed to address malaria and tuberculosis related problems. Laboratory confirmation improves disease management most efficiently within well-managed health laboratory systems. External quality assurance participation is associated with improved laboratory performance over time as it is a system for objectively checking a laboratory’s performance. However, many professionals in Sub Saharan Africa countries are unable to effectively implement a quality assurance program. This study aimed to assess the quality of smear microscopy in Guragae zone primary health care unit, Southern Ethiopia. Methods: Health institution-based cross-sectional study was conducted to recruit twenty-one primary health care units between May and August 2019. Blind rechecking was used to collect data. The sensitivity, specificity, positive predictive values, and negative predictive values were calculated by considering the final re-reading result as the gold standard. The level of agreement was measured using Kappa value. SPSS version 21 was used for data management and analysis. Result: A total of 860 and 318 stained slides for tuberculosis and malaria were collected, respectively. From total collected slides for tuberculosis, about 13.1 % of them were reported positive and from total malaria slides collected, half were positive for Plasmodium species at the peripheral laboratory while about 36.1% and 13% were confirmed positive for Plasmodium species and tuberculosis, respectively during re-reading at Wolkite University laboratory. Referring to the final result, the surveyed health facilities achieved “moderate agreement”(K=0.6) on malaria slide detection and “almost perfect agreement” (K=0.9) on slides for acid-fast bacilli. Only 4.4% of the surveyed health facilities incorporate malaria parasite count estimation in their report as per the current guideline. Conclusion: Now is the time to build sustainable laboratory capacity in resource-poor settings like Ethiopia that can be used to manage existing infectious diseases including malaria and tuberculosis. Malaria related technical problems were identified in this study. Thus, the authors believe that a continuous and strong malaria quality assurance schemes should be implemented at each laboratory to ensure reliable results.