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M.F. Andrés
Institute of Agricultural Sciences, CSIC, 28006 Madrid, Spain

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Journal article
Published: 12 May 2021 in Plants
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Cardenolide-free extracts from Digitalis obscura showed significant antifeedant effects against the aphid Myzus persicae and this activity correlated with their phenylethanoid content. The content in phenylethanoids of Digitalis obscura has been studied. Maceration of the aerial parts of D. obscura was used for the selective extraction of the natural compound rengyolone (1) and the aglycone of cornoside (compound 3). Pure rengyolone (1) can be obtained from D. obscura in approximately 90% purity from fresh plant from the CHCl3 soluble fraction of the ethanolic extract (0.8% yield). The ethanol extraction of freshly collected D. obscura showed the presence of compound 3 as the only phenylethanoid. Compound 3 was proven to easily evolve to rengyolone. Due to this instability, and although its presence in plants has been previously reported, the spectroscopical data of 3 are reported herein for the first time. Selective mono-acetylation of compound of 3 led to the active natural compound hallerone (5). The aphid antifeedant (against Myzus persicae) and nematicidal (against root-knot nematode Meloidogyne javanica) activities of these compounds have been evaluated. Here we report for the first time on the aphid antifeedant effects of 1, 3, and 5. Additionally, the nematicidal activity of hallerone (5) is described here for the first time.

ACS Style

José Quílez del Moral; Álvaro Pérez; María Navarro; Alberto Galisteo; Azucena Gonzalez-Coloma; María Andrés; Alejandro Barrero. Selective Extraction of Bioactive Phenylethanoids from Digitalis obscura. Plants 2021, 10, 959 .

AMA Style

José Quílez del Moral, Álvaro Pérez, María Navarro, Alberto Galisteo, Azucena Gonzalez-Coloma, María Andrés, Alejandro Barrero. Selective Extraction of Bioactive Phenylethanoids from Digitalis obscura. Plants. 2021; 10 (5):959.

Chicago/Turabian Style

José Quílez del Moral; Álvaro Pérez; María Navarro; Alberto Galisteo; Azucena Gonzalez-Coloma; María Andrés; Alejandro Barrero. 2021. "Selective Extraction of Bioactive Phenylethanoids from Digitalis obscura." Plants 10, no. 5: 959.

Journal article
Published: 02 February 2021 in Journal of Fungi
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In the current study, an ethyl acetate extract from the endophytic fungus Aspergillus sp. SPH2 isolated from the stem parts of the endemic plant Bethencourtia palmensis was screened for its biocontrol properties against plant pathogens (Fusarium moniliforme, Alternaria alternata , and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi), plant parasites (Meloidogyne javanica), and ticks (Hyalomma lusitanicum). SPH2 gave extracts with strong fungicidal and ixodicidal effects at different fermentation times. The bioguided isolation of these extracts gave compounds 1–3. Mellein (1) showed strong ixodicidal effects and was also fungicidal. This is the first report on the ixodicidal effects of 1. Neoaspergillic acid (2) showed potent antifungal effects. Compound 2 appeared during the exponential phase of the fungal growth while neohydroxyaspergillic acid (3) appeared during the stationary phase, suggesting that 2 is the biosynthetic precursor of 3. The mycotoxin ochratoxin A was not detected under the fermentation conditions used in this work. Therefore, SPH2 could be a potential biotechnological tool for the production of ixodicidal extracts rich in mellein.

ACS Style

Viridiana Morales-Sánchez; Carmen E. Díaz; Elena Trujillo; Sonia A. Olmeda; Felix Valcarcel; Rubén Muñoz; María Fe Andrés; Azucena González-Coloma. Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2. Journal of Fungi 2021, 7, 109 .

AMA Style

Viridiana Morales-Sánchez, Carmen E. Díaz, Elena Trujillo, Sonia A. Olmeda, Felix Valcarcel, Rubén Muñoz, María Fe Andrés, Azucena González-Coloma. Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2. Journal of Fungi. 2021; 7 (2):109.

Chicago/Turabian Style

Viridiana Morales-Sánchez; Carmen E. Díaz; Elena Trujillo; Sonia A. Olmeda; Felix Valcarcel; Rubén Muñoz; María Fe Andrés; Azucena González-Coloma. 2021. "Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2." Journal of Fungi 7, no. 2: 109.

Preprint
Published: 16 December 2020
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In the current study, an ethyl acetate extract from the endophytic fungus Aspergillus ochraceus SPH2 isolated from the stem parts of the endemic plant Bethencourtia palmensis was screened for its biocontrol properties against plant pathogens (Fusarium moniliforme, Alternaria alternata and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi), plant parasites (Meloidogyne javanica) and ticks (Hyalomma lusitanicum). SPH2 gave extracts with strong fungicidal and ixodicidal effects at different fermentation times. The bioguided isolation of these extracts gave compounds 1-3. Mellein (1) showed strong ixodicidal effects and was also fungicidal. This is the first report on the ixodicidal effects of 1. Neoaspergillic acid (2) showed potent antifungal effects. Compound 2 appeared during the exponential phase of the fungal growth while neohydroxyaspergillic acid (3) appeared during the stationary phase, suggesting that 2 is the biosynthetic precursor of 3. Additional molecular ions compatible with pyrazynes that were detected during the exponential phase included flavacol, and aspergilliamide while ochramide A was mostly detected during the stationary phase of the fermentation. Moreover, polyketids were also detected during the sationaty phase of the fermentation curve (dihydroaspyrone, aspyrone, asperlactone) and the alkaloid circumdatin H. Ochratoxin A was not detected. Therefore, SPH2 could be a potential biotechnological tool for the production of ixodicidal mellein.

ACS Style

Viridiana Morales-Sánchez; Carmen E. Díaz; Elena Trujillo; Rubén Muñoz; A. Sonia Olmeda; Felix Valcarcel; Maria Fe Andrés; Azucena Gonzalez-Coloma. Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2. 2020, 1 .

AMA Style

Viridiana Morales-Sánchez, Carmen E. Díaz, Elena Trujillo, Rubén Muñoz, A. Sonia Olmeda, Felix Valcarcel, Maria Fe Andrés, Azucena Gonzalez-Coloma. Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2. . 2020; ():1.

Chicago/Turabian Style

Viridiana Morales-Sánchez; Carmen E. Díaz; Elena Trujillo; Rubén Muñoz; A. Sonia Olmeda; Felix Valcarcel; Maria Fe Andrés; Azucena Gonzalez-Coloma. 2020. "Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2." , no. : 1.

Journal article
Published: 17 March 2020 in Microorganisms
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Botanical and fungal biopesticides, including endophytes, are in high demand given the current restrictive legislations on the use of chemical pesticides. As part of an ongoing search for new biopesticides, a series of fungal endophytes have been isolated from selected medicinal plants including Lauraceae species. In the current study, an extract from the endophytic fungus Trichoderma sp. EFI 671, isolated from the stem parts of the medicinal plant Laurus sp., was screened for bioactivity against plant pathogens (Fusarium graminearum, Rhizoctonia solani, Sclerotinia sclerotiorum and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi) and plant parasites (Meloidogyne javanica), with positive results against M. persicae. The chemical study of the neutral fraction of the active hexane extract resulted in the isolation of a triglyceride mixture (m1), eburicol (2), β-sitostenone (3), ergosterol (4) and ergosterol peroxide (5). The free fatty acids present in the acid fraction of the extract and in m1 (oleic, linoleic, palmitic and stearic) showed strong dose-dependent antifeedant effects against M. persicae. Liquid (potato dextrose broth, PDB and Sabouraud Broth, SDB) and solid (corn, sorghum, pearl millet and rice) growth media were tested in order to optimize the yield and bioactivity of the fungal extracts. Pearl millet and corn gave the highest extract yields. All the extracts from these solid media had strong effects against M. persicae, with sorghum being the most active. Corn media increased the methyl linoleate content of the extract, pearl millet media increased the oleic acid and sorghum media increased the oleic and linoleic acids compared to rice. The antifeedant effects of these extracts correlated with their content in methyl linoleate and linoleic acid. The phytotoxic effects of these extracts against ryegrass, Lolium perenne, and lettuce, Lactuca sativa, varied with culture media, with sorghum being non- toxic.

ACS Style

Nutan Kaushik; Carmen E. Díaz; Hemraj Chhipa; L. Fernando Julio; M. Fe Andrés; Azucena González-Coloma. Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671. Microorganisms 2020, 8, 420 .

AMA Style

Nutan Kaushik, Carmen E. Díaz, Hemraj Chhipa, L. Fernando Julio, M. Fe Andrés, Azucena González-Coloma. Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671. Microorganisms. 2020; 8 (3):420.

Chicago/Turabian Style

Nutan Kaushik; Carmen E. Díaz; Hemraj Chhipa; L. Fernando Julio; M. Fe Andrés; Azucena González-Coloma. 2020. "Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671." Microorganisms 8, no. 3: 420.

Regular article
Published: 04 March 2020 in New Phytologist
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Root‐knot nematodes (RKNs; Meloidogyne spp.) induce new post‐embryogenic organs within the roots (galls) were they stablish and differentiate nematode’s feeding cells, giant‐cells (GCs). Developmental programs and functional genes involved are scarcely defined. Arabidopsis root apical meristem (RAM), lateral root (LR) and callus marker lines: SHORT‐ROOT/SHR, SCARECROW/SCR, SCHIZORIZA/SCZ, WUSCHEL‐RELATED‐HOMEOBOX‐5/WOX5, AUXIN‐RESPONSIVE‐FACTOR‐5/ARF5, ARABIDOPSIS‐HISTIDINE PHOSPHOTRANSFER‐PROTEIN‐6/AHP6, GATA‐TRANSCRIPTION FACTOR‐23/GATA23, S‐PHASE‐KINASE‐ASSOCIATED‐PROTEIN2B/SKP2B were analysed for nematode‐dependent expression. Their corresponding loss‐of‐function lines, including those for LR upstream regulators: SOLITARY ROOT/SLR/IAA14, BONDELOS/BDL/IAA12 and INDOLE‐3‐ACETIC‐ACID‐INDUCIBLE‐28/IAA28 were tested for RKNs resistance/tolerance. LR genes, e.g. ARF5 (key factor for root stem‐cell niche regeneration), GATA23 (that specify pluripotent founder cells), and AHP6 (cytokinin‐signalling‐inhibitor regulating pericycle cell‐divisions orientation), show a crucial function during gall formation. RKNs do not compromise the number of founder cells or LR primordia but locally induce gall formation possibly by tuning the auxin/cytokinin balance where AHP6 might be necessary. Key RAM marker genes were induced and functional in galls. Therefore, the activation of plant developmental programs promoting transient‐pluripotency/stemness leads to the generation of quiescent‐centre and meristematic‐like cell identities within the vascular cylinder of galls. Nematodes enlist developmental pathways of new‐organogenesis and/or root regeneration in the vascular cells of galls. This should determine meristematic cell identities with enough transient‐pluripotency for gall organogenesis.

ACS Style

Rocío Olmo; Javier Cabrera; Fernando E. Díaz‐Manzano; Virginia Ruiz‐Ferrer; Marta Barcala; Takashi Ishida; Alejandra García; María Fe Andrés; Simón Ruiz‐Lara; Isabel Verdugo; Mónica Pernas; Hidehiro Fukaki; Juan Carlos Del Pozo; Miguel Ángel Moreno‐Risueno; Tina Kyndt; Godelieve Gheysen; Carmen Fenoll; Shinichiro Sawa; Carolina Escobar. Root‐knot nematodes induce gall formation by recruiting developmental pathways of post‐embryonic organogenesis and regeneration to promote transient pluripotency. New Phytologist 2020, 227, 200 -215.

AMA Style

Rocío Olmo, Javier Cabrera, Fernando E. Díaz‐Manzano, Virginia Ruiz‐Ferrer, Marta Barcala, Takashi Ishida, Alejandra García, María Fe Andrés, Simón Ruiz‐Lara, Isabel Verdugo, Mónica Pernas, Hidehiro Fukaki, Juan Carlos Del Pozo, Miguel Ángel Moreno‐Risueno, Tina Kyndt, Godelieve Gheysen, Carmen Fenoll, Shinichiro Sawa, Carolina Escobar. Root‐knot nematodes induce gall formation by recruiting developmental pathways of post‐embryonic organogenesis and regeneration to promote transient pluripotency. New Phytologist. 2020; 227 (1):200-215.

Chicago/Turabian Style

Rocío Olmo; Javier Cabrera; Fernando E. Díaz‐Manzano; Virginia Ruiz‐Ferrer; Marta Barcala; Takashi Ishida; Alejandra García; María Fe Andrés; Simón Ruiz‐Lara; Isabel Verdugo; Mónica Pernas; Hidehiro Fukaki; Juan Carlos Del Pozo; Miguel Ángel Moreno‐Risueno; Tina Kyndt; Godelieve Gheysen; Carmen Fenoll; Shinichiro Sawa; Carolina Escobar. 2020. "Root‐knot nematodes induce gall formation by recruiting developmental pathways of post‐embryonic organogenesis and regeneration to promote transient pluripotency." New Phytologist 227, no. 1: 200-215.

Preprint
Published: 21 November 2019
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Increasing pesticide resistance in plant pathogens is major concern in agriculture production. Research on ecofriendly alternatives of chemical pesticides are more in demand in pesticide industry. In the current study, an ethyl acetate extract from the endophytic fungus Trichoderma sp. EFI 671, isolated from the stem parts of the medicinal plant Laurus sp., was screened for bioactivity against plant pathogens (Fusarium graminearum, Rhizoctonia solani, Sclerotinia sclerotiorum and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi) and plant parasites (Meloidogyne javanica). The bioactive components have been characterized following a bioassay-guided isolation against M. persicae. The chemical study of this bioactive extract resulted in the isolation of 1-oleoyl-2-linoleoyl-3-palmitoylglycerol (1), eburicol (2), (24R)-stigmast-4-ene-3-one or β-sitostenone (3), ergosterol (4) and ergosterol peroxide (5). The free fatty acids present in compound 1 (oleic, linoleic and palmitic) showed strong dose-dependent aphid antifeedant effects against M. persicae. Liquid (PDB, and SDB) and solid (corn, sorghum, pearl millet and rice) growth media were tested in order to optimize the yield and bioactivity of the fungal extracts. Pearl millet and corn gave the highest extract yields. All the extracts from these solid media had strong effects against M. persicae with sorghum being the most active. Corn increased the content in linolenic, pearl millet the oleic and stearic and sorghum oleic and linolenic acids compared to rice. Their antifeedant effects correlated with linoleic /oleic acids. The phytotoxic effects of these extracts against Lolium perenne and Lactuca sativa varied with culture media, with sorghum being the least toxic.

ACS Style

Nutan Kaushik; Carmen E. Diaz; Hemraj Chhipa; L. Fernando Julio; Maria Fe Andres; Azucena Gonzalez-Coloma. Aphid Antifeedant Fatty Acids from an Endophytic Fungus Trichoderma sp. EFI671. 2019, 1 .

AMA Style

Nutan Kaushik, Carmen E. Diaz, Hemraj Chhipa, L. Fernando Julio, Maria Fe Andres, Azucena Gonzalez-Coloma. Aphid Antifeedant Fatty Acids from an Endophytic Fungus Trichoderma sp. EFI671. . 2019; ():1.

Chicago/Turabian Style

Nutan Kaushik; Carmen E. Diaz; Hemraj Chhipa; L. Fernando Julio; Maria Fe Andres; Azucena Gonzalez-Coloma. 2019. "Aphid Antifeedant Fatty Acids from an Endophytic Fungus Trichoderma sp. EFI671." , no. : 1.

Journal article
Published: 02 October 2019 in Biomolecules
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Given the importance of the genus Artemisia as a source of valuable natural products, the rare plant Artemisia pedemontana subspecies assoana, endemic to the Iberian Peninsula, has been experimentally cultivated in the greenhouse and aeroponically, to produce biomass for essential oil (EO) extraction. The chemical composition of the EOs was analyzed, and their plant protection (insects: Spodoptera littoralis, Rhopalosiphum padi, and Myzus persicae; plants: Lactuca sativa and Lolium perenne; fungi: Aspergillus niger; and nematode: Meloidogyne javanica) and antiparasitic (Trypanosoma cruzi, Phytomonas davidi, and antiplasmodial by the ferriprotoporphyrin biocrystallization inhibition test) properties were studied, in addition to the hydrolate by-product. The EOs showed a 1,8-cineole and camphor profile, with quantitative and qualitative chemical differences between the cultivation methods. These oils had moderate insect antifeedant, antifungal, and phytotoxic effects; were trypanocidel; and exhibited moderate phytomonacidal effects, while the hydrolate showed a strong nematicidal activity. Both EOs were similarly antifeedant; the EO from the greenhouse plants (flowering stage) was more biocidal (antifungal, nematicidal, and phytotoxic) than the EO from the aeroponic plants (growing stage), which was more antiparasitic. The major components of the oils (1,8-cineole and camphor), or their 1:1 combination, did not explain any of these effects. We can conclude that these EOs have potential applications as insect antifeedants, and as antifungal or antiparasitic agents, depending on the cultivation method, and that the hydrolate byproduct is a potent nematicidal.

ACS Style

Paula Sainz; María Fe Andrés; Martínez- Díaz; María Bailén; Juliana Navarro Rocha; Rafael A. Martínez-Díaz; Azucena González-Coloma. Chemical Composition and Biological Activities of Artemisia pedemontana subsp. assoana Essential Oils and Hydrolate. Biomolecules 2019, 9, 558 .

AMA Style

Paula Sainz, María Fe Andrés, Martínez- Díaz, María Bailén, Juliana Navarro Rocha, Rafael A. Martínez-Díaz, Azucena González-Coloma. Chemical Composition and Biological Activities of Artemisia pedemontana subsp. assoana Essential Oils and Hydrolate. Biomolecules. 2019; 9 (10):558.

Chicago/Turabian Style

Paula Sainz; María Fe Andrés; Martínez- Díaz; María Bailén; Juliana Navarro Rocha; Rafael A. Martínez-Díaz; Azucena González-Coloma. 2019. "Chemical Composition and Biological Activities of Artemisia pedemontana subsp. assoana Essential Oils and Hydrolate." Biomolecules 9, no. 10: 558.

Articles
Published: 28 August 2019 in Journal of Essential Oil Research
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The present work reports the effect of salt treatment on Hyssopus officinalis growth, essential oil (EO) yield and composition and the antiparasitic effects of the oil against the plant (Phytomonas davidi and Meloidogyne javanica) and human (Trypanosoma cruzi) parasites. Our results showed that salinity decreased plant growth and EO yield of hydroponically cultivated plants of H. officinalis with increasing NaCl levels. Salinity also induced changes in the chemical composition of treated hyssop EOs, decreasing the monoterpene and increasing the sesquiterpene groups. The oxygenated monoterpenes, represented primarily by isopinocamphone and pinocarvone, were the major chemical class. The EOs from treated H. officinalis showed moderate antiparasitic effects against T. cruzi, independent of the salt treatment, stronger salt-dependent effects on P. davidi and also a high salt-dependent nematicidal activity against M. javanica. Therefore, a moderate salt treatment reduced plant growth and EO yield but increased its biocidal effects on plant parasites (P. davidi and M. javanica).

ACS Style

Nesrine Ben Hamida; Rafael A. Martínez-Díaz; MahmoudI Hela; Kamel Msaada; Zeineb Ouerghi; Maria Fe Andres; Azucena González-Coloma. Effect of salinity on the antiparasitic activity of hyssop essential oil. Journal of Essential Oil Research 2019, 32, 69 -78.

AMA Style

Nesrine Ben Hamida, Rafael A. Martínez-Díaz, MahmoudI Hela, Kamel Msaada, Zeineb Ouerghi, Maria Fe Andres, Azucena González-Coloma. Effect of salinity on the antiparasitic activity of hyssop essential oil. Journal of Essential Oil Research. 2019; 32 (1):69-78.

Chicago/Turabian Style

Nesrine Ben Hamida; Rafael A. Martínez-Díaz; MahmoudI Hela; Kamel Msaada; Zeineb Ouerghi; Maria Fe Andres; Azucena González-Coloma. 2019. "Effect of salinity on the antiparasitic activity of hyssop essential oil." Journal of Essential Oil Research 32, no. 1: 69-78.

Journal article
Published: 03 May 2019 in Molecules
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Eugenia winzerlingii (Myrtaceae) is an endemic plant from the Yucatan peninsula. Its organic extracts and fractions from leaves have been tested on two phloem-feeding insects, Bemisia tabaci and Myzus persicae, on two plant parasitic nematodes, Meloidogyne incognita and Meloidogyne javanica, and phytotoxicity on Lolium perenne and Solanum lycopersicum. Results showed that both the hexane extract and the ethyl acetate extract, as well as the fractions, have strong antifeedant and nematicidal effects. Gas chromatography-mass spectrometry analyses of methylated active fractions revealed the presence of a mixture of fatty acids. Authentic standards of detected fatty acids and methyl and ethyl derivatives were tested on target organisms. The most active compounds were decanoic, undecanoic, and dodecanoic acids. Methyl and ethyl ester derivatives had lower effects in comparison with free fatty acids. Dose-response experiments showed that undecanoic acid was the most potent compound with EC50 values of 21 and 6 nmol/cm2 for M. persicae and B. tabaci, respectively, and 192 and 64 nmol for M. incognita and M. javanica, respectively. In a phytotoxicity assay, medium-chain fatty acids caused a decrease of 38-52% in root length and 50-60% in leaf length of L. perenne, but no effects were observed on S.lycopersicum. This study highlights the importance of the genus Eugenia as a source of bioactive metabolites for plant pest management.

ACS Style

Angel Cruz-Estrada; Esaú Ruiz-Sánchez; Jairo Cristóbal-Alejo; Azucena González-Coloma; María Fe Andrés; Marcela Gamboa-Angulo. Medium-Chain Fatty Acids from Eugenia winzerlingii Leaves Causing Insect Settling Deterrent, Nematicidal, and Phytotoxic Effects. Molecules 2019, 24, 1724 .

AMA Style

Angel Cruz-Estrada, Esaú Ruiz-Sánchez, Jairo Cristóbal-Alejo, Azucena González-Coloma, María Fe Andrés, Marcela Gamboa-Angulo. Medium-Chain Fatty Acids from Eugenia winzerlingii Leaves Causing Insect Settling Deterrent, Nematicidal, and Phytotoxic Effects. Molecules. 2019; 24 (9):1724.

Chicago/Turabian Style

Angel Cruz-Estrada; Esaú Ruiz-Sánchez; Jairo Cristóbal-Alejo; Azucena González-Coloma; María Fe Andrés; Marcela Gamboa-Angulo. 2019. "Medium-Chain Fatty Acids from Eugenia winzerlingii Leaves Causing Insect Settling Deterrent, Nematicidal, and Phytotoxic Effects." Molecules 24, no. 9: 1724.

Journal article
Published: 22 April 2019 in Toxins
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Cantharidin (CTD) is a toxic monoterpene produced by blister beetles (Fam. Meloidae) as a chemical defense against predators. Although CTD is highly poisonous to many predator species, some have evolved the ability to feed on poisonous Meloidae, or otherwise beneficially use blister beetles. Great Bustards, Otis tarda, eat CTD-containing Berberomeloe majalis blister beetles, and it has been hypothesized that beetle consumption by these birds reduces parasite load (a case of self-medication). We examined this hypothesis by testing diverse organisms against CTD and extracts of B. majalis hemolymph and bodies. Our results show that all three preparations (CTD and extracts of B. majalis) were toxic to a protozoan (Trichomonas vaginalis), a nematode (Meloidogyne javanica), two insects (Myzus persicae and Rhopalosiphum padi) and a tick (Hyalomma lusitanicum). This not only supports the anti-parasitic hypothesis for beetle consumption, but suggests potential new roles for CTD, under certain conditions.

ACS Style

Douglas W. Whitman; Maria Fe Andrés; Rafael A. Martínez-Díaz; Alexandra Ibáñez-Escribano; A. Sonia Olmeda; Azucena González-Coloma; Martínez- Díaz; Ibáñez- Escribano; González- Coloma. Antiparasitic Properties of Cantharidin and the Blister Beetle Berberomeloe majalis (Coleoptera: Meloidae). Toxins 2019, 11, 234 .

AMA Style

Douglas W. Whitman, Maria Fe Andrés, Rafael A. Martínez-Díaz, Alexandra Ibáñez-Escribano, A. Sonia Olmeda, Azucena González-Coloma, Martínez- Díaz, Ibáñez- Escribano, González- Coloma. Antiparasitic Properties of Cantharidin and the Blister Beetle Berberomeloe majalis (Coleoptera: Meloidae). Toxins. 2019; 11 (4):234.

Chicago/Turabian Style

Douglas W. Whitman; Maria Fe Andrés; Rafael A. Martínez-Díaz; Alexandra Ibáñez-Escribano; A. Sonia Olmeda; Azucena González-Coloma; Martínez- Díaz; Ibáñez- Escribano; González- Coloma. 2019. "Antiparasitic Properties of Cantharidin and the Blister Beetle Berberomeloe majalis (Coleoptera: Meloidae)." Toxins 11, no. 4: 234.

Full paper
Published: 03 November 2017 in New Phytologist
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Root knot nematodes (RKNs) penetrate into the root vascular cylinder, triggering morphogenetic changes to induce galls, de novo formed ‘pseudo‐organs’ containing several giant cells (GCs). Distinctive gene repression events observed in early gall/GCs development are thought to be mediated by post‐transcriptional silencing via microRNAs (miRNAs), a process that is far from being fully characterized. Arabidopsis thaliana backgrounds with altered activities based on target 35S::MIMICRY172 (MIM172), 35S::TARGET OF EARLY ACTIVATION TAGGED 1 (TOE1)‐miR172‐resistant (35S::TOE1R) and mutant (flowering locus T‐10 (ft‐10)) lines were used for functional analysis of nematode infective and reproductive parameters. The GUS‐reporter lines, MIR172A–E::GUS, treated with auxin (IAA) and an auxin‐inhibitor (a‐(phenyl ethyl‐2‐one)‐indole‐3‐acetic acid (PEO‐IAA)), together with the MIR172C AuxRE::GUS line with two mutated auxin responsive elements (AuxREs), were assayed for nematode‐dependent gene expression. Arabidopsis thaliana backgrounds with altered expression of miRNA172, TOE1 or FT showed lower susceptibility to the RKNs and smaller galls and GCs. MIR172C−D::GUS showed restricted promoter activity in galls/GCs that was regulated by auxins through auxin‐responsive factors. IAA induced their activity in galls while PEO‐IAA treatment and mutations in AuxRe motifs abolished it. The results showed that the regulatory module miRNA172/TOE1/FT plays an important role in correct GCs and gall development, where miRNA172 is modulated by auxins.

ACS Style

Fernando E. Díaz-Manzano; Javier Cabrera; Juan-José Ripoll; Iván Del Olmo; Maria Fe Andres; Ana Cláudia Silva; Marta Barcala; María Sánchez; Virginia Ruíz-Ferrer; Janice De Almeida-Engler; Martin F. Yanofsky; Manuel Piñeiro; Jose Antonio Jarillo; Carmen Fenoll; Carolina Escobar. A role for the gene regulatory modulemicroRNA172/TARGET OF EARLY ACTIVATION TAGGED 1/FLOWERING LOCUS T(miRNA172/TOE1/FT) in the feeding sites induced byMeloidogyne javanicainArabidopsis thaliana. New Phytologist 2017, 217, 813 -827.

AMA Style

Fernando E. Díaz-Manzano, Javier Cabrera, Juan-José Ripoll, Iván Del Olmo, Maria Fe Andres, Ana Cláudia Silva, Marta Barcala, María Sánchez, Virginia Ruíz-Ferrer, Janice De Almeida-Engler, Martin F. Yanofsky, Manuel Piñeiro, Jose Antonio Jarillo, Carmen Fenoll, Carolina Escobar. A role for the gene regulatory modulemicroRNA172/TARGET OF EARLY ACTIVATION TAGGED 1/FLOWERING LOCUS T(miRNA172/TOE1/FT) in the feeding sites induced byMeloidogyne javanicainArabidopsis thaliana. New Phytologist. 2017; 217 (2):813-827.

Chicago/Turabian Style

Fernando E. Díaz-Manzano; Javier Cabrera; Juan-José Ripoll; Iván Del Olmo; Maria Fe Andres; Ana Cláudia Silva; Marta Barcala; María Sánchez; Virginia Ruíz-Ferrer; Janice De Almeida-Engler; Martin F. Yanofsky; Manuel Piñeiro; Jose Antonio Jarillo; Carmen Fenoll; Carolina Escobar. 2017. "A role for the gene regulatory modulemicroRNA172/TARGET OF EARLY ACTIVATION TAGGED 1/FLOWERING LOCUS T(miRNA172/TOE1/FT) in the feeding sites induced byMeloidogyne javanicainArabidopsis thaliana." New Phytologist 217, no. 2: 813-827.

Journal article
Published: 01 November 2017 in Food and Chemical Toxicology
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In this study we evaluated the effect of a pressure gradient (1-2 atm) in the extraction and composition of the essential oil (EO) of Piper hispidinervum by steam distillation. We also evaluated the insect antifeedant effects (Spodoptera littoralis, Leptinotarsa decemlineata, Myzus persicae and Rhopalosiphum padi) and nematicidal activity (Meloidogyne javanica) of the oils, their major components and their synergistic interactions. Safrole was the major component (78-81%) followed by terpinolene (5-9%). The EOs tested were effective insect antifeedants. Safrole, explained most of the insect antifeedant action of P. hispidinervum EOs. When safrole and terpinolene were tested in binary combinations, low ratios of safrole improved the antifeedant effects of terpinolene. P. hispidinervum EOs caused higher mortality of M. javanica juveniles than their major components. In binary combinations, low ratios of terpinolene increased the nematicidal effects of safrole. The EO treatment strongly suppressed nematode egg hatching and juvenile infectivity. P. hispidinervum EOs affected the germination of S. lycopersicum and L. sativa mostly at 24 h of treatment, being L. sativa the most sensitive. Safrole moderately affected germination and root growth of L. sativa, S. lycopersicum and L. perenne. Terpinolene only affected S. lycopersicum root growth.

ACS Style

M.F. Andrés; G.E. Rossa; E. Cassel; R.M.F. Vargas; O. Santana; C.E. Díaz; A. González-Coloma. Biocidal effects of Piper hispidinervum (Piperaceae) essential oil and synergism among its main components. Food and Chemical Toxicology 2017, 109, 1086 -1092.

AMA Style

M.F. Andrés, G.E. Rossa, E. Cassel, R.M.F. Vargas, O. Santana, C.E. Díaz, A. González-Coloma. Biocidal effects of Piper hispidinervum (Piperaceae) essential oil and synergism among its main components. Food and Chemical Toxicology. 2017; 109 ():1086-1092.

Chicago/Turabian Style

M.F. Andrés; G.E. Rossa; E. Cassel; R.M.F. Vargas; O. Santana; C.E. Díaz; A. González-Coloma. 2017. "Biocidal effects of Piper hispidinervum (Piperaceae) essential oil and synergism among its main components." Food and Chemical Toxicology 109, no. : 1086-1092.

Full paper
Published: 12 August 2017 in Chemistry & Biodiversity
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This work presents the biocidal (insecticidal, ixodicidal, nematicidal, and phytotoxic) effects and chemical compositions of three essential oils obtained from the industrial steam distillation (IEOs) of hyssop (Hyssopus officinalis L.), lavandin (Lavandula × intermedia or L. × hybrida var. Super), and cotton lavender (Santolina chamaecyparissus L.). Their chemical composition analyzed by gas chromatography coupled to mass spectrometry showed 1,8‐cineole (53%) and β‐pinene (16%) as the major components of H. officinalis, linalyl acetate (38%) and linalool (29%) of L. × intermedia; and 1,8‐cineole (10%) and 8‐methylene‐3‐oxatricyclo[5.2.0.02,4]nonane (8%) in S. chamaecyparissus. The biocidal tests showed that L. × intermedia IEO was the most active against the insect Spodoptera littoralis and toxic to the tick Hyalomma lusitanicum, IEO of H. officinalis was strongly active against S. littoralis, and finally, S. chamaecyparissus IEO was a strong antifeedant against the aphid Rhopalosiphum padi, toxic to H. lusitanicum and with moderate effects against Leptinotarsa decemlineata, S. littoralis, and Lolium perenne.

ACS Style

Gonzalo Ortiz De Elguea-Culebras; Raúl Sánchez-Vioque; María Isabel Berruga; David Herraiz-Peñalver; Azucena González-Coloma; Maria Fe Andres; Omar Santana‐Méridas. Biocidal Potential and Chemical Composition of Industrial Essential Oils from Hyssopus officinalis , Lavandula × intermedia var. Super , and Santolina chamaecyparissus. Chemistry & Biodiversity 2017, 15, e1700313 .

AMA Style

Gonzalo Ortiz De Elguea-Culebras, Raúl Sánchez-Vioque, María Isabel Berruga, David Herraiz-Peñalver, Azucena González-Coloma, Maria Fe Andres, Omar Santana‐Méridas. Biocidal Potential and Chemical Composition of Industrial Essential Oils from Hyssopus officinalis , Lavandula × intermedia var. Super , and Santolina chamaecyparissus. Chemistry & Biodiversity. 2017; 15 (1):e1700313.

Chicago/Turabian Style

Gonzalo Ortiz De Elguea-Culebras; Raúl Sánchez-Vioque; María Isabel Berruga; David Herraiz-Peñalver; Azucena González-Coloma; Maria Fe Andres; Omar Santana‐Méridas. 2017. "Biocidal Potential and Chemical Composition of Industrial Essential Oils from Hyssopus officinalis , Lavandula × intermedia var. Super , and Santolina chamaecyparissus." Chemistry & Biodiversity 15, no. 1: e1700313.

Journal article
Published: 22 June 2017 in Environmental Science and Pollution Research
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The nematicidal activity of hydrolate by-products from the semi industrial vapor-pressure essential oil extraction of selected aromatic plant species (commercial: Lavandula × intermedia Emeric ex Loisel. var. super, Thymus vulgaris L., T. zygis Loefl ex L. and experimentally pre-domesticated: L. luisieri (Rozeira) Rivas-Martínez) was investigated against the root-knot nematode Meloidogyne javanica by in vitro and in vivo bioassays. Liquid-liquid extraction of hydrolates yielded the corresponding aqueous and organic fractions which were biological and chemically studied. Hydrolates from L. × intermedia var. super, L. luisieri, T. vulgaris, and T. zygis showed strong in vitro nematicidal effects against M. javanica (J2 mortality and suppression of egg hatching). In the case of the Thymus species, the active components were found in the organic fraction, characterized by thymol as major component. Conversely, the nematicidal activity of L. × intermedia var. super and L. luisieri remained in the corresponding aqueous fractions. In vivo tests on tomato seedlings at sublethal doses of the hydrolates/organic fractions induced a significant reduction of nematode infectivity. In pot experiments, all hydrolates tested on tomato plants significantly affect the infection frequency and reproduction rate of the nematode population. This study demonstrates that L. × intermedia var. super, L. luisieri, T. vulgaris, and T. zygis hydrolates could be an exploitable source of potential waste protection products on root-knot nematodes.

ACS Style

Maria Fe Andrés; Azucena González-Coloma; Ruben Muñoz; Felipe De La Peña; Luis Fernando Julio; Jesus Burillo. Nematicidal potential of hydrolates from the semi industrial vapor-pressure extraction of Spanish aromatic plants. Environmental Science and Pollution Research 2017, 25, 29834 -29840.

AMA Style

Maria Fe Andrés, Azucena González-Coloma, Ruben Muñoz, Felipe De La Peña, Luis Fernando Julio, Jesus Burillo. Nematicidal potential of hydrolates from the semi industrial vapor-pressure extraction of Spanish aromatic plants. Environmental Science and Pollution Research. 2017; 25 (30):29834-29840.

Chicago/Turabian Style

Maria Fe Andrés; Azucena González-Coloma; Ruben Muñoz; Felipe De La Peña; Luis Fernando Julio; Jesus Burillo. 2017. "Nematicidal potential of hydrolates from the semi industrial vapor-pressure extraction of Spanish aromatic plants." Environmental Science and Pollution Research 25, no. 30: 29834-29840.

Article
Published: 24 May 2017 in Phytochemistry Reviews
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Endophytes fungi have been widely bioprospected to find new drugs and drug leads including antimicrobial agents and antifungals. However, an important role in host plant protection has been suggested for their presence and their metabolites. Therefore, nematicidal and insecticidal effects of their metabolites should be expected. In this review, the literature data available on insecicidal and nematicidal compounds identified from fungal endophytes are presented. Additionally we present a recent study on the endophytic biodiversity of a unique paleoflora, the Macaronesian laurel forest, in the light of their role in plant protection.

ACS Style

Maria Fe Andrés; Carmen Elisa Diaz; Cristina Giménez; Raimundo Cabrera; Azucena Gonzalez-Coloma. Endophytic fungi as novel sources of biopesticides: the Macaronesian Laurel forest, a case study. Phytochemistry Reviews 2017, 16, 1009 -1022.

AMA Style

Maria Fe Andrés, Carmen Elisa Diaz, Cristina Giménez, Raimundo Cabrera, Azucena Gonzalez-Coloma. Endophytic fungi as novel sources of biopesticides: the Macaronesian Laurel forest, a case study. Phytochemistry Reviews. 2017; 16 (5):1009-1022.

Chicago/Turabian Style

Maria Fe Andrés; Carmen Elisa Diaz; Cristina Giménez; Raimundo Cabrera; Azucena Gonzalez-Coloma. 2017. "Endophytic fungi as novel sources of biopesticides: the Macaronesian Laurel forest, a case study." Phytochemistry Reviews 16, no. 5: 1009-1022.

Journal article
Published: 01 April 2017 in Crop Protection
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Luis F. Julio; Azucena Gonzalez-Coloma; Jesus Burillo; Carmen E. Diaz; Maria Fe Andrés. Nematicidal activity of the hydrolate byproduct from the semi industrial vapor pressure extraction of domesticated Artemisia absinthium against Meloidogyne javanica. Crop Protection 2017, 94, 33 -37.

AMA Style

Luis F. Julio, Azucena Gonzalez-Coloma, Jesus Burillo, Carmen E. Diaz, Maria Fe Andrés. Nematicidal activity of the hydrolate byproduct from the semi industrial vapor pressure extraction of domesticated Artemisia absinthium against Meloidogyne javanica. Crop Protection. 2017; 94 ():33-37.

Chicago/Turabian Style

Luis F. Julio; Azucena Gonzalez-Coloma; Jesus Burillo; Carmen E. Diaz; Maria Fe Andrés. 2017. "Nematicidal activity of the hydrolate byproduct from the semi industrial vapor pressure extraction of domesticated Artemisia absinthium against Meloidogyne javanica." Crop Protection 94, no. : 33-37.

Article
Published: 18 February 2017 in Chemistry & Biodiversity
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Essential oils from Greek Mentha species showed different chemical compositions for two populations of M. pulegium, characterized by piperitone and pulegone. Mentha spicata essential oil was characterized by endocyclic piperitenone epoxide, piperitone epoxide, and carvone. The bioactivities of these essential oils and their components have been tested against insect pests (Leptinotarsa decemlineata, Spodoptera littoralis and Myzus persicae), root-knot nematodes (Meloydogine javanica) and plants (Lactuca sativa, Lolium perenne, Solanum lycopersicum). The structure–activity relationships of these compounds have been studied including semi-synthetic endocyclic trans-carvone epoxide, exocyclic carvone epoxide, a new exocyclic piperitenone epoxide and trans-pulegone epoxide. Leptinotarsa decemlineata feeding was affected by piperitenone and piperitone epoxide. Spodoptera littoralis was affected by piperitone epoxide and pulegone. The strongest nematicidal agent was piperitenone epoxide, followed by piperitone epoxide, piperitenone and carvone. Germination of S. lycopersicum and L. perenne was significantly affected by piperitenone epoxide. This compound and carvone epoxide inhibited L. perenne root and leaf growth. Piperitenone epoxide also inhibited the root growth of S. lycopersicum. The presence of a C(1) epoxide resulted in strong antifeedant, nematicidal and phytotoxic compounds regardless of the C(4) substituent. New natural crop protectants could be developed through appropriate structural modifications in the p-menthane skeleton.

ACS Style

Athanasios C. Kimbaris; Azucena González‐Coloma; Maria Fe Andres; Veroniki P. Vidali; Moschos G. Polissiou; Omar Santana‐Méridas. Biocidal Compounds fromMenthasp. Essential Oils and Their Structure-Activity Relationships. Chemistry & Biodiversity 2017, 14, e1600270 .

AMA Style

Athanasios C. Kimbaris, Azucena González‐Coloma, Maria Fe Andres, Veroniki P. Vidali, Moschos G. Polissiou, Omar Santana‐Méridas. Biocidal Compounds fromMenthasp. Essential Oils and Their Structure-Activity Relationships. Chemistry & Biodiversity. 2017; 14 (3):e1600270.

Chicago/Turabian Style

Athanasios C. Kimbaris; Azucena González‐Coloma; Maria Fe Andres; Veroniki P. Vidali; Moschos G. Polissiou; Omar Santana‐Méridas. 2017. "Biocidal Compounds fromMenthasp. Essential Oils and Their Structure-Activity Relationships." Chemistry & Biodiversity 14, no. 3: e1600270.

Journal article
Published: 01 January 2017 in Revista Argentina de Microbiología
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Fungal metabolites are promising alternatives for the development of biorational pesticides. In this sense, microfungi from tropical regions are valuable sources of natural compounds for pest management. With the aim of broadening the search for new eco-friendly products to manage plant pests, this study was carried out to evaluate the biological activity of 23 tropical fungal extracts on three species of phytophagous insects and a plant parasitic nematode. In addition, the active principles of the most effective extract were identified. The insect deterrent activity of fungal extracts was evaluated on the settling of aphids Myzus persicae and Rhopalosiphum padi, and on the feeding of lepidoptera larva Spodoptera littoralis; the nematostatic activity was evaluated on the mobility of Meloidogyne javanica. Active metabolites from Gliomastix masseei were identified by GC-MS techniques and by comparison with commercial standards. Results showed seven extracts with strong effect on the settling of M. persicae and R. padi (settling inhibition >80%). The calculated median of effective concentration (EC) values ranged from 8 to 38μg/cm for the extracts of Clonostachys rosea and G. masseei, respectively. Bioassay-guided separation of the ethyl acetate extract of G. masseei revealed the presence of fatty acids and their derivatives, where methyl 9-octadecenoate was the most active compound with EC values of 16μg and 35μg/cm for M. persicae and R. padi, respectively. Extracts of C. rosea and G. masseei could be a promising option in the control of pest aphids in agriculture.

ACS Style

Ana L. Ruiz-Jiménez; Azucena Gonzalez-Coloma; Maria Fe Andres; Esaú Ruiz-Sánchez; Gabriela Heredia; Sergio R. Peraza-Sánchez; Irma L. Medina-Baizabal; María Manuela De Jesús Reyes Estebanez; Blondy Canto-Canché; Marcela Gamboa-Angulo. Insect deterrent and nematicidal screening of microfungi from Mexico and anti-aphid compounds from Gliomastix masseei. Revista Argentina de Microbiología 2017, 49, 83 -92.

AMA Style

Ana L. Ruiz-Jiménez, Azucena Gonzalez-Coloma, Maria Fe Andres, Esaú Ruiz-Sánchez, Gabriela Heredia, Sergio R. Peraza-Sánchez, Irma L. Medina-Baizabal, María Manuela De Jesús Reyes Estebanez, Blondy Canto-Canché, Marcela Gamboa-Angulo. Insect deterrent and nematicidal screening of microfungi from Mexico and anti-aphid compounds from Gliomastix masseei. Revista Argentina de Microbiología. 2017; 49 (1):83-92.

Chicago/Turabian Style

Ana L. Ruiz-Jiménez; Azucena Gonzalez-Coloma; Maria Fe Andres; Esaú Ruiz-Sánchez; Gabriela Heredia; Sergio R. Peraza-Sánchez; Irma L. Medina-Baizabal; María Manuela De Jesús Reyes Estebanez; Blondy Canto-Canché; Marcela Gamboa-Angulo. 2017. "Insect deterrent and nematicidal screening of microfungi from Mexico and anti-aphid compounds from Gliomastix masseei." Revista Argentina de Microbiología 49, no. 1: 83-92.

Journal article
Published: 01 March 2016 in Crop Protection
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Heterodera avenae and other cyst-producing nematode species are traditionally counted manually by visual inspection, a laborious and time consuming process. A method to detect and count cereal cyst nematodes based on image analysis techniques and statistical identification was designed and tested on H. avenae white females. Nematodes at the white female stage and free of soil particles were isolated from a contaminated wheat field. Known quantities of white females were mixed with different pre-treated background soil samples from non infested fields. Pre-treatment consisted of typical sieving and centrifugation procedures for white female extraction. Image analysis techniques were then used to detect, count and characterize white females in the mixtures. Over 96% of the H. avenae white females and substrate particles were correctly identified showing that a clear distinction can be drawn between the two groups. The results confirmed the validity of the proposed method to identify and count H. avenae females in backgrounds of different origins. This study establishes the basis for the design of a standardized method that is easily incorporated into a stand-alone system to facilitate the detection and quantification of nematode inoculum. This method can be applied to evaluate infestation level in crop fields and also to detect resistance/tolerance in breeding lines.

ACS Style

Oscar Grillo; Maria Fe Andres; Santiago Moreno-Vázquez. Quantitative evaluation of Heterodera avenae females in soil and root extracts by digital image analysis. Crop Protection 2016, 81, 85 -91.

AMA Style

Oscar Grillo, Maria Fe Andres, Santiago Moreno-Vázquez. Quantitative evaluation of Heterodera avenae females in soil and root extracts by digital image analysis. Crop Protection. 2016; 81 ():85-91.

Chicago/Turabian Style

Oscar Grillo; Maria Fe Andres; Santiago Moreno-Vázquez. 2016. "Quantitative evaluation of Heterodera avenae females in soil and root extracts by digital image analysis." Crop Protection 81, no. : 85-91.

Review article
Published: 22 January 2016 in Journal of Natural Products
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Several preparations were obtained from the aerial parts of predomesticated Lavandula luisieri, including the essential oil and ethanolic, hexane, and ethyl acetate extractives. Additionally, pilot plant vapor pressure extraction was carried out at a pressure range of 0.5–1.0 bar to give a vapor pressure oil and an aqueous residue. A chemical study of the hexane extract led to the isolation of six necrodane derivatives (1, 2, and 4–7), with four of these (1, 2, 5, and 7) being new, as well as camphor, a cadinane sesquiterpene (9), tormentic acid, and ursolic acid. The EtOAc and EtOH extracts contained a mixture of phenolic compounds with rosmarinic acid being the major component. Workup of the aqueous residue resulted in the isolation of the necrodane 3 and (1R*,2S*,4R*)-p-menth-5-ene-1,2,8-triol (8), both new natural compounds. The structures of the new compounds were established based on their spectroscopic data. The phytotoxic and nematicidal activities of these compounds were evaluated.

ACS Style

Luis F. Julio; Alejandro Fernandez Barrero; M. Mar Herrador Del Pino; Jesús F Arteaga; Jesús Burillo; Maria Fe Andres; Carmen E. Díaz; Azucena Gonzalez-Coloma. Phytotoxic and Nematicidal Components of Lavandula luisieri. Journal of Natural Products 2016, 79, 261 -266.

AMA Style

Luis F. Julio, Alejandro Fernandez Barrero, M. Mar Herrador Del Pino, Jesús F Arteaga, Jesús Burillo, Maria Fe Andres, Carmen E. Díaz, Azucena Gonzalez-Coloma. Phytotoxic and Nematicidal Components of Lavandula luisieri. Journal of Natural Products. 2016; 79 (2):261-266.

Chicago/Turabian Style

Luis F. Julio; Alejandro Fernandez Barrero; M. Mar Herrador Del Pino; Jesús F Arteaga; Jesús Burillo; Maria Fe Andres; Carmen E. Díaz; Azucena Gonzalez-Coloma. 2016. "Phytotoxic and Nematicidal Components of Lavandula luisieri." Journal of Natural Products 79, no. 2: 261-266.