This page has only limited features, please log in for full access.

Unclaimed
Daniela Pasotto
Department of Animal Medicine, Production and Health (MAPS), University of Padova, 35020 Legnaro, Padova, Italy

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 26 January 2021 in Animals
Reads 0
Downloads 0

Anaplasma phagocytophilum (A. phagocytophilum) is a tick-borne pathogen causing disease in both humans and animals. Human granulocytic anaplasmosis (HGA) is an emerging disease, but despite the remarkable prevalence in European ticks and wild animals, human infection appears underdiagnosed. Several genetic variants are circulating in Europe, including the zoonotic ecotype I. This study investigated A. phagocytophilum occurrence in wild ungulates and their ectoparasites in an area where HGA has been reported. Blood samples from wild ungulates and ectoparasites were screened by biomolecular methods targeting the mps2 gene. The groEL gene was amplified and sequenced to perform genetic characterization and phylogenetic analysis. A total of 188 blood samples were collected from different wild ungulates species showing an overall prevalence of 63.8% (88.7% in wild ruminants and 3.6% in wild boars). The prevalence of A. phagocytophilum DNA in ticks (manly Ixodes ricinus), and keds collected from wild ruminants was high, reflecting the high infection rates obtained in their hosts. Among ticks collected from wild boars (Hyalomma marginatum and Dermacentor marginatus) no DNA was detected. Phylogenetic analysis demonstrated the presence of ecotype I and II. To date, this is the first Italian report of ecotype I in alpine chamois, mouflon, and wild boar species. These findings suggest their role in HGA epidemiology, and the high prevalence detected in this study highlights that this human tick-borne disease deserves further attention.

ACS Style

Laura Grassi; Giovanni Franzo; Marco Martini; Alessandra Mondin; Rudi Cassini; Michele Drigo; Daniela Pasotto; Elena Vidorin; Maria Menandro. Ecotyping of Anaplasma phagocytophilum From Wild Ungulates and Ticks Shows Circulation of Zoonotic Strains in Northeastern Italy. Animals 2021, 11, 310 .

AMA Style

Laura Grassi, Giovanni Franzo, Marco Martini, Alessandra Mondin, Rudi Cassini, Michele Drigo, Daniela Pasotto, Elena Vidorin, Maria Menandro. Ecotyping of Anaplasma phagocytophilum From Wild Ungulates and Ticks Shows Circulation of Zoonotic Strains in Northeastern Italy. Animals. 2021; 11 (2):310.

Chicago/Turabian Style

Laura Grassi; Giovanni Franzo; Marco Martini; Alessandra Mondin; Rudi Cassini; Michele Drigo; Daniela Pasotto; Elena Vidorin; Maria Menandro. 2021. "Ecotyping of Anaplasma phagocytophilum From Wild Ungulates and Ticks Shows Circulation of Zoonotic Strains in Northeastern Italy." Animals 11, no. 2: 310.

Journal article
Published: 07 January 2021 in Pathogens
Reads 0
Downloads 0

Differently from alpha- and betaherpesviruses affecting swine, interest in the recently discovered Suid gammaherpesvirus 3, Suid gammaherpesvirus 4, and Suid gammaherpesvirus 5, also known as porcine lymphotropic herpesviruses (PLHV-1, PLHV-2, and PLHV-3), has largely focused on their role as potential zoonotic agents in cases of xenotransplantation. However, their role as primary pathogens of swine or as co-factors for other lymphotropic infections has essentially been neglected. The present study aims at filling this gap, evaluating the association between PLHVs infection and different clinical conditions and/or porcine circovirus (PCV) co-infection. One hundred seventy-six samples were obtained from different animals located in a high-density pig area of Northern Italy in the period 2017–2020. The presence of PLHVs and PCVs was tested and quantified by specific real-time PCR: PLHVs were widespread among pigs (PLHV-1, PLHV-2, and PLHV-3 prevalence was 28.97%, 10.79%, and 4.54%, respectively) and detected in all considered tissues and clinical conditions. Frequent co-infections were also observed among PLHVs and with PCVs, although a significant association was not detected with the exception of a positive interaction between PLHV-1 and PLHV-3, and a negative one between PLHV-2 and PCV-2. Significantly, no association between PLHVs, alone or in co-infection, emerged with any of the considered clinical signs, their frequency being comparable between healthy and diseased animals. Based on these pieces of evidence and despite their high prevalence, PLHVs’ relevance for the swine industry appears negligible, either as primary pathogens or as predisposing factors for circovirus-induced diseases.

ACS Style

Giovanni Franzo; Michele Drigo; Matteo Legnardi; Laura Grassi; Maria Luisa Menandro; Daniela Pasotto; Mattia Cecchinato; Claudia Maria Tucciarone. Porcine Gammaherpesviruses in Italian Commercial Swine Population: Frequent but Harmless. Pathogens 2021, 10, 47 .

AMA Style

Giovanni Franzo, Michele Drigo, Matteo Legnardi, Laura Grassi, Maria Luisa Menandro, Daniela Pasotto, Mattia Cecchinato, Claudia Maria Tucciarone. Porcine Gammaherpesviruses in Italian Commercial Swine Population: Frequent but Harmless. Pathogens. 2021; 10 (1):47.

Chicago/Turabian Style

Giovanni Franzo; Michele Drigo; Matteo Legnardi; Laura Grassi; Maria Luisa Menandro; Daniela Pasotto; Mattia Cecchinato; Claudia Maria Tucciarone. 2021. "Porcine Gammaherpesviruses in Italian Commercial Swine Population: Frequent but Harmless." Pathogens 10, no. 1: 47.

Journal article
Published: 10 November 2020 in Viruses
Reads 0
Downloads 0

Bovine coronavirus (BoCV) is an important pathogen of cattle, causing severe enteric disease and playing a role in the bovine respiratory disease complex. Similar to other coronaviruses, a remarkable variability characterizes both its genome and biology. Despite their potential relevance, different aspects of the evolution of BoCV remain elusive. The present study reconstructs the history and evolution of BoCV using a phylodynamic approach based on complete genome and spike protein sequences. The results demonstrate high mutation and recombination rates affecting different parts of the viral genome. In the spike gene, this variability undergoes significant selective pressures—particularly episodic pressure—located mainly on the protein surface, suggesting an immune-induced selective pressure. The occurrence of compensatory mutations was also identified. On the contrary, no strong evidence in favor of host and/or tissue tropism affecting viral evolution has been proven. The well-known plasticity is thus ascribable to the innate broad viral tropism rather than mid- or long-term adaptation. The evaluation of the geographic spreading pattern clearly evidenced two clusters: a European cluster and an American–Asian cluster. While a relatively dense and quick migration network was identified in the former, the latter was dominated by the primary role of the United States (US) as a viral exportation source. Since the viral spreading pattern strongly mirrored the cattle trade, the need for more intense monitoring and preventive measures cannot be underestimated as well as the need to enforce the vaccination of young animals before international trade, to reduce not only the clinical impact but also the transferal and mixing of BoCV strains.

ACS Style

Giovanni Franzo; Michele Drigo; Matteo Legnardi; Laura Grassi; Daniela Pasotto; Maria Luisa Menandro; Mattia Cecchinato; Claudia Maria Tucciarone. Bovine Coronavirus: Variability, Evolution, and Dispersal Patterns of a No Longer Neglected Betacoronavirus. Viruses 2020, 12, 1285 .

AMA Style

Giovanni Franzo, Michele Drigo, Matteo Legnardi, Laura Grassi, Daniela Pasotto, Maria Luisa Menandro, Mattia Cecchinato, Claudia Maria Tucciarone. Bovine Coronavirus: Variability, Evolution, and Dispersal Patterns of a No Longer Neglected Betacoronavirus. Viruses. 2020; 12 (11):1285.

Chicago/Turabian Style

Giovanni Franzo; Michele Drigo; Matteo Legnardi; Laura Grassi; Daniela Pasotto; Maria Luisa Menandro; Mattia Cecchinato; Claudia Maria Tucciarone. 2020. "Bovine Coronavirus: Variability, Evolution, and Dispersal Patterns of a No Longer Neglected Betacoronavirus." Viruses 12, no. 11: 1285.

Journal article
Published: 28 October 2020 in International Journal of Environmental Research and Public Health
Reads 0
Downloads 0

Animal-assisted interventions (AAIs) are based on the establishment of a therapeutic relationship between animals and beneficiaries that is certain to provide positive effects, while currently, it reads as if AAIs aim at exposing stakeholders to potential risk of infection. The surveillance of zoonotic pathogens is necessary for guaranteeing common health. This study investigated the presence of potentially zoonotic parasites, including dermatophytes, in animals involved in AAIs. Between 2015 and 2017, 190 animals (equids, dogs, cats, birds, rabbits, rodents, and goats) were investigated. Anamnestic and management data were recorded. Individual faecal samples were analysed using a copromicroscopic procedure. Fur and skin were examined for ectoparasites during clinical examinations, and samples for mycological investigation were collected by brushing. Parasites were described in 60 (31.6%) investigated animals. Thirteen out of the 60 (21.7%) animals harboured potentially zoonotic parasites, mainly recovered in dogs (Ancylostomatidae, Eucoleus aerophilus, Toxocara canis, and Giardia duodenalis) and a cat (G. duodenalis). Nannizzia gypsea and Paraphyton mirabile, potential agents of cutaneous mycosis, were isolated in a dog and a horse, respectively. No ectoparasites were found. AAIs might represent a source of infections either directly or via environmental contamination. Thus, active surveillance is necessary and animal screenings should be planned and scheduled according to the risk of exposure.

ACS Style

Giulia Simonato; Patrizia Danesi; Antonio Frangipane Di Regalbono; Giorgia Dotto; Cinzia Tessarin; Mario Pietrobelli; Daniela Pasotto. Surveillance of Zoonotic Parasites in Animals Involved in Animal-Assisted Interventions (AAIs). International Journal of Environmental Research and Public Health 2020, 17, 7914 .

AMA Style

Giulia Simonato, Patrizia Danesi, Antonio Frangipane Di Regalbono, Giorgia Dotto, Cinzia Tessarin, Mario Pietrobelli, Daniela Pasotto. Surveillance of Zoonotic Parasites in Animals Involved in Animal-Assisted Interventions (AAIs). International Journal of Environmental Research and Public Health. 2020; 17 (21):7914.

Chicago/Turabian Style

Giulia Simonato; Patrizia Danesi; Antonio Frangipane Di Regalbono; Giorgia Dotto; Cinzia Tessarin; Mario Pietrobelli; Daniela Pasotto. 2020. "Surveillance of Zoonotic Parasites in Animals Involved in Animal-Assisted Interventions (AAIs)." International Journal of Environmental Research and Public Health 17, no. 21: 7914.

Journal article
Published: 17 March 2020 in Pathogens
Reads 0
Downloads 0

Porcine circovirus 2 (PCV-2) is one of the most impactful and widespread pathogens of the modern swine industry. Unlike other DNA viruses, PCV-2 is featured by a remarkable genetic variability, which has led to the emergence and recognition of different genotypes, some of which (PCV-2a, 2b, and 2d) have alternated over time. Currently, PCV-2d is considered the most prevalent genotype, and some evidence of differential virulence and vaccine efficacy have been reported. Despite the potential practical relevance, the data on PCV-2 epidemiology in Italy are quite outdated and do not quantify the actual circulation of this genotype in Italy. In the present study, 82 complete ORF2 sequences were obtained from domestic pigs and wild boars sampled in Northern Italy in the period 2013–2018 and merged with those previously obtained from Italy and other countries. A combination of phylogenetic, haplotype network, and phylodynamic analyses were used to genotype the collected strains and evaluate the temporal trend and the spatial and host spread dynamics. A rising number of PCV-2d detections was observed in domestic pigs, particularly since 2013, reaching a detection frequency comparable to PCV-2b. A similar picture was observed in wild boars, although a lower sequence number was available. Overall, the present study demonstrates the extreme complexity of PCV-2 molecular epidemiology in Italy, the significant spread across different regions, the recurrent introduction from foreign countries, and the frequent occurrence of recombination events. Although a higher viral flux occurred from domestic to wild populations than vice versa, wild boars seem to maintain PCV-2 infection and spread it over relatively long distances.

ACS Style

Giovanni Franzo; Susanna Tinello; Laura Grassi; Claudia Maria Tucciarone; Matteo Legnardi; Mattia Cecchinato; Giorgia Dotto; Alessandra Mondin; Marco Martini; Daniela Pasotto; Maria Luisa Menandro; Michele Drigo. Free to Circulate: An Update on the Epidemiological Dynamics of Porcine Circovirus 2 (PCV-2) in Italy Reveals the Role of Local Spreading, Wild Populations, and Foreign Countries. Pathogens 2020, 9, 221 .

AMA Style

Giovanni Franzo, Susanna Tinello, Laura Grassi, Claudia Maria Tucciarone, Matteo Legnardi, Mattia Cecchinato, Giorgia Dotto, Alessandra Mondin, Marco Martini, Daniela Pasotto, Maria Luisa Menandro, Michele Drigo. Free to Circulate: An Update on the Epidemiological Dynamics of Porcine Circovirus 2 (PCV-2) in Italy Reveals the Role of Local Spreading, Wild Populations, and Foreign Countries. Pathogens. 2020; 9 (3):221.

Chicago/Turabian Style

Giovanni Franzo; Susanna Tinello; Laura Grassi; Claudia Maria Tucciarone; Matteo Legnardi; Mattia Cecchinato; Giorgia Dotto; Alessandra Mondin; Marco Martini; Daniela Pasotto; Maria Luisa Menandro; Michele Drigo. 2020. "Free to Circulate: An Update on the Epidemiological Dynamics of Porcine Circovirus 2 (PCV-2) in Italy Reveals the Role of Local Spreading, Wild Populations, and Foreign Countries." Pathogens 9, no. 3: 221.

Journal article
Published: 04 July 2019 in Comparative Immunology, Microbiology and Infectious Diseases
Reads 0
Downloads 0

The aim of this study was to assess the prevalence, the genotypic diversity, the antimicrobial resistance traits of canine and feline clinical methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates in a diagnostic laboratory in Italy during 2015-2016. All isolates were characterized by multilocus sequence typing (MLST), staphylococcal cassette chromosome (SCC)-mec typing and staphylococcal protein A (spa)-typing. The resistance profiles were assessed by antimicrobial susceptibility testing and confirmed genotypically by the detection of mecA gene and by microarray analyses. The prevalence of MRSP isolates was high (31.6%). All the strains were multidrug resistant and the most frequent clone was ST71-SCCmec type II-III. These results confirm a high prevalence of MRSP amongst clinical samples from pets in Italy. These isolates show multidrug resistance features that are of concern both in veterinary and human medicine for clinical and epidemiological reasons.

ACS Style

Maria Luisa Menandro; Giorgia Dotto; Alessandra Mondin; Marco Martini; Letizia Ceglie; Daniela Pasotto. Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types. Comparative Immunology, Microbiology and Infectious Diseases 2019, 66, 101331 .

AMA Style

Maria Luisa Menandro, Giorgia Dotto, Alessandra Mondin, Marco Martini, Letizia Ceglie, Daniela Pasotto. Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types. Comparative Immunology, Microbiology and Infectious Diseases. 2019; 66 ():101331.

Chicago/Turabian Style

Maria Luisa Menandro; Giorgia Dotto; Alessandra Mondin; Marco Martini; Letizia Ceglie; Daniela Pasotto. 2019. "Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types." Comparative Immunology, Microbiology and Infectious Diseases 66, no. : 101331.

Journal article
Published: 01 January 2013 in BMC Veterinary Research
Reads 0
Downloads 0

To evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was processed by a double centrifugation tube method to obtain platelet concentrates, which were then analyzed by a flow cytometry haematology system for haemogram. Platelet concentration and white blood cell count were determined in all samples.

ACS Style

Anna Perazzi; Roberto Busetto; Tiziana Martinello; Michele Drigo; Daniela Pasotto; Francesco Cian; Marco Patruno; Ilaria Iacopetti. Description of a double centrifugation tube method for concentrating canine platelets. BMC Veterinary Research 2013, 9, 146 -146.

AMA Style

Anna Perazzi, Roberto Busetto, Tiziana Martinello, Michele Drigo, Daniela Pasotto, Francesco Cian, Marco Patruno, Ilaria Iacopetti. Description of a double centrifugation tube method for concentrating canine platelets. BMC Veterinary Research. 2013; 9 (1):146-146.

Chicago/Turabian Style

Anna Perazzi; Roberto Busetto; Tiziana Martinello; Michele Drigo; Daniela Pasotto; Francesco Cian; Marco Patruno; Ilaria Iacopetti. 2013. "Description of a double centrifugation tube method for concentrating canine platelets." BMC Veterinary Research 9, no. 1: 146-146.