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Ascorbic acid (AsA) and glutathione (GSH) are considered important factors to protect plants against abiotic stress. To investigate whether altered endogenous GSH and AsA affect seed germination, plant performance and the abiotic stress tolerance, GSH deficient mutant cad2-1 and AsA-deficient mutants (vtc2-4 and vtc5-2) were phenotypically characterized for their seed germination, shoot growth, photosynthetic activity and root architecture under abiotic stresses. The cad2-1, vtc2-4 and vtc5-2 mutants showed a decrease in osmotic and salt stress tolerance, in sensitivity to ABA during seed germination, and in plant performance under severe abiotic stresses. GSH deficiency in the cad2-1 plants affected plant growth and root development in plants exposed to strong drought, oxidative and heavy metal stress conditions. Plants with lower GSH did not show an increased sensitivity to strong salt stress (100 mM NaCl). In contrast, the mutants with lower AsA enhanced salt stress tolerance in the long-term exposures to strong salt stress since they showed larger leaf areas, longer primary roots and more lateral root numbers. Limitations on AsA or GSH synthesis had no effect on photosynthesis in plants exposed to long-term strong salt or drought stresses, whereas they effected on photosynthesis of mutants exposed to CdCl2. Taken together, the current study suggests that AsA and GSH are important for seed germination, root architecture, shoot growth and plant performance in response to different abiotic stresses, and their functions are dependent on the stress-inducing agents and the stress levels.
Minh Hoang; Mai Doan; Thuong Nguyen; Dong-Phuong Tra; Thanh Chu; Thi Dang; Phuong Quach. Phenotypic Characterization of Arabidopsis Ascorbate and Glutathione Deficient Mutants under Abiotic Stresses. Agronomy 2021, 11, 764 .
AMA StyleMinh Hoang, Mai Doan, Thuong Nguyen, Dong-Phuong Tra, Thanh Chu, Thi Dang, Phuong Quach. Phenotypic Characterization of Arabidopsis Ascorbate and Glutathione Deficient Mutants under Abiotic Stresses. Agronomy. 2021; 11 (4):764.
Chicago/Turabian StyleMinh Hoang; Mai Doan; Thuong Nguyen; Dong-Phuong Tra; Thanh Chu; Thi Dang; Phuong Quach. 2021. "Phenotypic Characterization of Arabidopsis Ascorbate and Glutathione Deficient Mutants under Abiotic Stresses." Agronomy 11, no. 4: 764.
White mulberry (Morus alba L.) root was reported to contain potential tyrosinase inhibitors such as norartocarpetin, streppogenin, oxyresveratrol (5, 7, 2′, 4′-tetrahydroxy-flavavone) and 2-arylbenzofuran. This study aims at investigating Morus alba L. hairy root culture to create a source of tyrosinase inhibitors for skin-whitening application. Morus alba L. leaf (including mesophyll and petiole) and stem explants were randomly wounded and inoculated with Agrobacterium rhizogenes ATCC 15834 in 10-40 minutes, and coculturing for 4 days. After inoculation, Agrobacterium rhizogenes were removed by using MS medium adding 200mg/L cefotaxime as antibiotics. Then, fresh inducted hairy roots were grown in nonphytohormone liquid medium (MS, White, Gamborg B5 or WPM) on a shaker (80rpm) at 25 ±1°C. Inducted hairy roots were tested for the present of rolB by PCR analysis and its effect on tyrosinase activity using a 96-well plate reader. The suitable explants for hairy root induction are leaves, especially petioles in 10 minutes (root induction frequency: 83%). After induction, hairy roots on MS was grown more efficiently (increased 3.933 times in FW) than on WPM, B5 and White medium. Inducted hairy roots were successfully transgenes by the present of rolB in their genome and had the same anti-tyrosinase activity as the natural roots without significance differences (IC50: 3.860 and 3.597 μg/mL, respectively). Cultured Morus alba L. hairy roots are potential source of tyrosinase inhibitors for future skin-whitening ingredient.
Hanh Thi My Nguyen; Tan Quoc Tran; Anh Lan Bui; Phuong Ngo Diem Quach. Hairy root culture of white mulberry (Morus alba L.) for a source of tyrosinase inhibitors. Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale 2020, 93, 1 .
AMA StyleHanh Thi My Nguyen, Tan Quoc Tran, Anh Lan Bui, Phuong Ngo Diem Quach. Hairy root culture of white mulberry (Morus alba L.) for a source of tyrosinase inhibitors. Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale. 2020; 93 (1):1.
Chicago/Turabian StyleHanh Thi My Nguyen; Tan Quoc Tran; Anh Lan Bui; Phuong Ngo Diem Quach. 2020. "Hairy root culture of white mulberry (Morus alba L.) for a source of tyrosinase inhibitors." Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale 93, no. 1: 1.
To overcome the problems in liverwort collecting such as small size and easily mixed with other species in the wild, we have successfully cultivated Marchantia polymorpha L. under in vitro conditions in the previous study. The aim of this study is to evaluate the biological activities of this in vitro biomass as a confirmation of the sufficient protocol in cultivation this species. Cultured biomass was dried at a temperature of 45-50 oC to constant weight and ground into a fine powder. The coarse powder was extracted with organic solvents of increasing polarization including n-hexane, chloroform, ethyl acetate, and ethanol using the maceration technique. Four extracts were investigated antioxidant (iron reduction power, DPPH), antibacterial (agar diffusion), tyrosinase inhibitory activity, anti-proliferation on MCF-7 cells. Additionally, the presence of natural metabolite groups of the extracts was detected by using specific reagents. For antioxidant activity, ethyl acetate fraction extract had the highest iron reducing power and DPPH free radical scavenging ability with IC50 = 439.31 µg ml-1. All three n-hexane, chloroform, and ethyl acetate extracts possessed resistance to the bacterial strain tested. At a concentration of 2 mg ml-1, n-hexane and chloroform extracts had the highest percentage of tyrosinase inhibition (69.54 and 69.10%, respectively). The n-hexane extract is a potent extract that inhibits the proliferation of MCF-7 cells with the lowest IC50 of 38.15 µg ml-1. A preliminary chemical composition survey showed that the cultured biomass liverwort contains many bioactive compounds, particularly the compounds of range of non- and less-polarized fractions.
Tan Q. Tran; Hoang N. Phan; Anh L. Bui; Phuong D. D. Quach. Biological activities of in vitro liverwort Marchantia polymorpha L. extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2020, 48, 826 -838.
AMA StyleTan Q. Tran, Hoang N. Phan, Anh L. Bui, Phuong D. D. Quach. Biological activities of in vitro liverwort Marchantia polymorpha L. extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020; 48 (2):826-838.
Chicago/Turabian StyleTan Q. Tran; Hoang N. Phan; Anh L. Bui; Phuong D. D. Quach. 2020. "Biological activities of in vitro liverwort Marchantia polymorpha L. extracts." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 48, no. 2: 826-838.
Aeroponics is considered as a potential method for the culture of herbal plants due to the high growth rate, quantity and quality enhancement of secondary metabolites, and substantial environmental progress associated with this method. The aim of this study was to develop a sufficient protocol for successful Urena lobata hairy root induction by Agrobacterium rhizogenes ATCC 15834, using a precursor and elicitor to enhance α-glucosidase inhibitory activity (GIA) of aeroponic hairy roots (AHRs) in greenhouse conditions. In this study, we found that the optimized procedure (10 min, Woody plant medium (WPM), 1/25 salt strength) had an outstanding effect with a reduction in the rooting time (RT), promotion of the rooting rate (RR), and increase in the fresh weight (FW) and dry weight (DW) compared with the original procedure (30 min, Murashige and Skoog (MS) medium, 1/25 salt strength) after 30 days of culture. The highest DW, GIA, flavonoid (FLA) and phenolic (PHEL) contents were observed for individual addition of 10 mM phenylalanine (PA) or 50 mM chitosan (CS) in the late exponential phase (eighth week) with 15 days of elicitation compared to the control AHRs. However, individual treatment was less effective than the combination of the two. Positive correlations among the GIA, FLA and PHEL indicate that AHRs accumulated phenolic compounds, leading to an increase in the GIA by a synergistic effect. In conclusion, the culture of Urena lobata AHRs with PA and CS is an efficient procedure to produce GIA material in greenhouse conditions.
Dai Minh Cao; Phuong Thi Bach Vu; Minh Thi Thanh Hoang; Anh Lan Bui; Phuong Ngo Diem Quach. Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor. Plants 2020, 9, 548 .
AMA StyleDai Minh Cao, Phuong Thi Bach Vu, Minh Thi Thanh Hoang, Anh Lan Bui, Phuong Ngo Diem Quach. Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor. Plants. 2020; 9 (4):548.
Chicago/Turabian StyleDai Minh Cao; Phuong Thi Bach Vu; Minh Thi Thanh Hoang; Anh Lan Bui; Phuong Ngo Diem Quach. 2020. "Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor." Plants 9, no. 4: 548.
Marchantia polymorpha L. is used as a folk medicine in India, China and some European countries for the treatment of inflammation, cure cuts, wounds, diuretics, etc. Natural compounds and biological activities of this liverwort have been studied in the world for few years. At present time, the studies on bryophytes (non-vascular plants) are quite limited, especially in Vietnam. Therefore, this research focused on investigating some biological activities and analyzing of major secondary metabolites of M. polymorpha L. collected at Da Lat, Lam Dong. Among four extracted fractions (nhexane, chloroform, ethyl acetate and ethanol), the chloroform extract exhibited the best antioxidant, antibacterial, anti-proliferation of MCF-7 cell line activities. The ethyl acetate extract exhibited better activity of tyrosinase and α-glucosidase inhibition than others. Main secondary compounds in this species were phenolics, steroids and glycosidederivatives. These results were scientific evidences that might help us to understand rigorously about the ultilization of Marchantia liverwort in the traditional treatment and new potential applications in the contemporary medicine.
Tan Quoc Tran; Tam Thien Luong; Hoang Ngo Phan; Phuong Ngo Diem Quach. Biological activities of the liverwort Marchantia polymorpha L. collected at Da Lat, Lam Dong province. Science and Technology Development Journal - Natural Sciences 2019, 2, 26 -34.
AMA StyleTan Quoc Tran, Tam Thien Luong, Hoang Ngo Phan, Phuong Ngo Diem Quach. Biological activities of the liverwort Marchantia polymorpha L. collected at Da Lat, Lam Dong province. Science and Technology Development Journal - Natural Sciences. 2019; 2 (5):26-34.
Chicago/Turabian StyleTan Quoc Tran; Tam Thien Luong; Hoang Ngo Phan; Phuong Ngo Diem Quach. 2019. "Biological activities of the liverwort Marchantia polymorpha L. collected at Da Lat, Lam Dong province." Science and Technology Development Journal - Natural Sciences 2, no. 5: 26-34.