Escherichia (E.) coli is a fecal microbe that inhabits the intestines of endotherms (primary habitat) and the natural environment (secondary habitats). Due to prevailing thinking regarding the limited capacity of E. coli to survive in the environment, relatively few published investigations exist regarding environmental factors influencing E. coli’s survival. To help guide future research in this area, an overview of factors known to impact the survival of E. coli in the environment is provided. Notably, the lack of historic field-based research holds two important implications: (1) large knowledge gaps regarding environmental factors influencing E. coli’s survival in the environment exist; and (2) the efficacy of implemented management strategies have rarely been assessed on larger field scales, thus leaving their actual impact(s) largely unknown. Moreover, the persistence of E. coli in the environment calls into question its widespread and frequent use as a fecal indicator microorganism. To address these shortcomings, future work should include more field-based studies, occurring in diverse physiographical regions and over larger spatial extents. This information will provide scientists and land-use managers with a new understanding regarding factors influencing E. coli concentrations in its secondary habitat, thereby providing insight to address problematic fecal contamination effectively.

The relationships between Escherichia (E) coli concentration, suspended particulate matter (SPM) particle size class, and land use practices are important in reducing the bacterium’s persistence and health risks. However, surprisingly few studies have been performed that quantify these relationships. Conceivably, such information would advance mitigation strategies for practices that address specific SPM size classes and, by proxy, E. coli concentration. To advance this needed area of research, stream water was sampled from varying dominant land use practices in West Run Watershed, a representative mixed-land use Appalachian watershed of West Virginia in the eastern USA. Water samples were filtered into three SPM intervals (60 μm) and the E. coli concentration (colony forming units, CFU) and SPM of each interval was quantified. Statistically significant relationships were identified between E. coli concentrations and size intervals (α < 0.0001), and SPM (α = 0.05). The results show a predominance (90% of total) of E. coli CFUs in the <5 μm SPM interval. The results show that land use practices impact the relationships between SPM and E. coli concentrations. Future work should include additional combined factors that influence bacterial CFUs and SPM, including hydrology, climate, geochemistry and nutrients.

Understanding mixed-land-use practices and physicochemical influences on Escherichia (E.) coli concentrations is necessary to improve water quality management and human health. Weekly stream water samples and physicochemical data were collected from 22 stream gauging sites representing varying land use practices in a contemporary Appalachian watershed of the eastern USA. Over the period of one annual year, Escherichia (E.) coli colony forming units (CFU) per 100 mL were compared to physicochemical parameters and land use practices. Annual average E. coli concentration increased by approximately 112% from acid mine drainage (AMD) impacted headwaters to the lower reaches of the watershed (approximate averages of 177 CFU per 100 mL vs. 376 CFU per 100 mL, respectively). Significant Spearman’s correlations (p < 0.05) were identified from analyses of pH and E. coli concentration data representing 77% of sample sites; thus highlighting legacy effects of historic mining (AMD) on microbial water quality. A tipping point of 25–30% mixed development was identified as leading to significant (p < 0.05) negative correlations between chloride and E. coli concentrations. Study results advance understanding of land use and physicochemical impacts on fecal contamination in mixed-land-use watersheds, aiding in the implementation of effective water quality management practices and policies.

Understanding land use practice induced increases in Escherichia (E.) coli and suspended particulate matter (SPM) concentrations is necessary to improve water quality. Weekly stream water samples were collected from 22 stream gauging sites with varying land use practices in a representative contemporary mixed-land use watershed of the eastern USA. Over the period of one annual year, Escherichia (E.) coli colony forming units (CFU per 100 mL) were compared to suspended particulate matter (SPM) concentrations (mg/L) and land use practices. Agricultural land use sub-catchments comprised elevated E. coli concentrations (avg. 560 CFU per 100 mL) compared to proximate mixed development (avg. 330 CFU per 100 mL) and forested (avg. 206 CFU per 100 mL) sub-catchments. Additionally, agricultural land use showed statistically significant relationships (p < 0.01) between annual E. coli and SPM concentration data. Quarterly PCA biplots displayed temporal variability in land use impacts on E. coli and SPM concentrations, with agricultural land use being closely correlated with both pollutants during Quarters 2 and 3 but not Quarters 1 and 4. The data collected during this investigation advance the understanding of land use impacts on fecal contamination in receiving waters, thereby informing land use managers on the best management practices to reduce exposure risks.

Barbuda is one of two major islands that comprise the Caribbean nation of Antigua and Barbuda. The island is known for its secluded pink and white sand beaches and, more recently, for Hurricane Irma (September 2017). The category five mega-storm decimated much of the island’s landscape and infrastructure, and the physical damage was widely publicized. Three years after Hurricane Irma, many challenges related to humanitarian aid, fiscal resources, and materials to rebuild remain. There are many natural resource commodity and human social challenges including those related to water resources, agriculture, marine ecosystems, feral animal populations, human health, tourism, and economics. This article includes some of Barbuda’s historical context and identifies a number of current critical challenges and recommendations for activities (approaches) that may advance a number of management practices. For example, the Integrated Watershed Management (IWM) and/or One Health approach(es) include social and fiscal mechanisms to implement programs, policies, legislation, and research in which multiple sectors of Barbuda could communicate and work together to achieve sustainable outcomes. Context is provided to substantiate humanitarian aid, scientific engagement, scientific progress, and political support for a semi-closed cultural socio-ecological island ecosystem at the precipice of change.