Canine distemper virus (CDV) has recently emerged as an extinction threat for the endangered Amur tiger (Panthera tigris altaica). CDV is vaccine-preventable, and control strategies could require vaccination of domestic dogs and/or wildlife populations. However, vaccination of endangered wildlife remains controversial, which has led to a focus on interventions in domestic dogs, often assumed to be the source of infection. Effective decision making requires an understanding of the true reservoir dynamics, which poses substantial challenges in remote areas with diverse host communities. We carried out serological, demographic, and phylogenetic studies of dog and wildlife populations in the Russian Far East to show that a number of wildlife species are more important than dogs, both in maintaining CDV and as sources of infection for tigers. Critically, therefore, because CDV circulates among multiple wildlife sources, dog vaccination alone would not be effective at protecting tigers. We show, however, that low-coverage vaccination of tigers themselves is feasible and would produce substantive reductions in extinction risks. Vaccination of endangered wildlife provides a valuable component of conservation strategies for endangered species. Significance The decline and progressive fragmentation of many threatened populations increase extinction vulnerability due to outbreaks of infectious disease. Vaccination is one of the few tools available to mitigate these threats, but its use is often hampered by insufficient epidemiological understanding and historic controversies over endangered wildlife vaccination. Using the example of Amur tigers and CDV, we describe a holistic approach to select appropriate disease mitigation strategies based on key epidemiological evidence from the field. We then assess the protection of vaccinated tigers against the locally circulating CDV strain and use modeling to compare the efficacy and cost of potential vaccination programs. This practical approach provides conservation managers with an evidence-based rationale to address disease-mediated extinction risks for threatened wildlife populations.

Tigers continue to face unprecedented threats to their existence due to poaching, habitat loss, habitat fragmentation and anthropogenic disturbances. The present study examines the physiological stress response of tigers due to anthropogenic activities including wildlife tourism in Bandhavgarh Tiger Reserve and Kanha Tiger Reserve using faecal glucocorticoid metabolite (fGCM) measurement. We collected a total of 341 faecal samples from both reserves during tourism and non-tourism periods. Data on various anthropogenic disturbances including tourism activities like number of vehicles and visitors were also collected. We ascertained the species identity and sex of all the samples collected using genetic markers. fGCMs were extracted using a previously reported procedure, and fGCM concentrations were subsequently determined using an established enzyme immunoassay. There was no significant difference in overall mean fGCM concentrations between the two tiger reserves, but within each reserve, concentrations were significantly higher in tigers during the tourism period as compared to the non-tourism period. We also found that the number of tourist vehicles and disturbance level significantly correlated with fGCM concentrations. This study further supports the assumption that unbridled tourism associated with high anthropogenic disturbance can be related to perceived stress and consequently may have an impact on the reproductive fitness of tigers and long-term survival of isolated populations.

Lactation is the most energetically expensive component of maternal care in mammals. Increased reproductive investment can lead to physiological stress for the mothers, based on the exhaustion of energy resources and increase in glucocorticoids level. This study aimed to estimate the changes in cortisol concentrations during lactation in domestic cats and compared the differences among litter sizes. Eleven females gave birth to 27 litters, which were divided in two groups—small (1–3 kittens) and large (4–7 kittens) litters. Blood samples were collected from each female before mating, after parturition, at 4 and 8 weeks of lactation. We showed that the cortisol level in females changed significantly during lactation—the highest concentrations were observed at the peak of lactation at 4 weeks. Cortisol levels varied significantly among females but did not depend on their maternal experience. We also revealed that there were no differences in cortisol levels between females with small and large litters, but at 4 weeks of lactation, the hormone concentrations were higher in females with small litters. It is likely that these females initially invested less in reproduction, giving birth to fewer offspring.

A noninvasive evaluation method of hypothalamo-pituitary-adrenals axis (HPA) activity in the Siberian tiger was verified. Comparison of the activity level of HPA in Siberian tigers in the wild and in captivity, and their alterations over the year was carried out. Significant seasonal differences between activity levels of HPA in tigers in captivity were not found. In the wild, this level was significantly higher, reaching the maximum from November to January, which can be related with an unfavorable influence on tigers in low temperatures and deep snow cover.

Seroprevalence to nine different virus pathogens was estimated for Russian big cats (Amur tiger (Panthera tigris altaica Temminck, 1844) and far-eastern leopard (Panthera pardus orientalis (Schiegel, 1857))) in Southern Primorie, Russia (n = 25), in 2008–2016. Serum samples from smaller cats (Eurasian lynx (Lynx lynx (Linnaeus, 1758)) and far-eastern wildcat (leopard cat) (Prionailurus bengalensis euptilurus (Elliot, 1871))) were also tested for these pathogens (n = 19) during the same period. Felids of Russian Southern Primorie showed seroprevalence to eight out of nine tested pathogens, including highly dangerous feline immunodeficiency virus, feline leukemia virus, and canine distemper virus. Antibodies to feline panleukopenia virus were found to be much more widespread in cats (45%) than antibodies to any other virus. They were detected in samples taken from tigers, leopards, and far-eastern wildcats but not lynxes. Antibodies to pseudorabies virus were detected only in Amur tiger (29%), whose main prey is the most common carrier of the virus (wild boar), unlike for the other studied cats’ species.