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Pseudorabies virus (PRV), an alphaherpesvirus, causes respiratory and reproductive diseases in pigs and severe nervous symptom in other susceptible hosts. Previous studies showed that PRV infection induced a systemic inflammatory response in mice, indicating that pro-inflammatory cytokines participated in viral neuropathy in mice. The pro-inflammatory cytokine IL-1β is a key mediator of the inflammatory response and plays an important role in host-response to pathogens. However, the secretion of IL-1β and its relationship with inflammasome activation during PRV infection remains poorly understood. In this study, we found that PRV infection caused significant secretion of several pro-inflammatory cytokines in macrophages and promoted IL-1β secretion in an ATP-dependent manner. Furthermore, the expression of IL-1β can be induced by only PRV infection and depended on NF-κB pathway activation, while the subsequent secretion of IL-1β was mediated by ATP-induced P2 × 7R activation, loss of intracellular K+, and the subsequent NLRP3 inflammasome activation. By using a mouse infection model, we also found that ATP exacerbated clinical signs and death of mice infected by PRV in a NLRP3-dependent manner. These results indicate that ATP facilitates activation of NLRP3 inflammasome and enhances the pathogenicity of PRV in mice during its acute infection.
Chao Ye; Qingyuan Huang; Jiali Jiang; Gang Li; Dongyi Xu; Zheng Zeng; Lianci Peng; Yuanyi Peng; Rendong Fang. ATP-dependent activation of NLRP3 inflammasome in primary murine macrophages infected by pseudorabies virus. Veterinary Microbiology 2021, 259, 109130 .
AMA StyleChao Ye, Qingyuan Huang, Jiali Jiang, Gang Li, Dongyi Xu, Zheng Zeng, Lianci Peng, Yuanyi Peng, Rendong Fang. ATP-dependent activation of NLRP3 inflammasome in primary murine macrophages infected by pseudorabies virus. Veterinary Microbiology. 2021; 259 ():109130.
Chicago/Turabian StyleChao Ye; Qingyuan Huang; Jiali Jiang; Gang Li; Dongyi Xu; Zheng Zeng; Lianci Peng; Yuanyi Peng; Rendong Fang. 2021. "ATP-dependent activation of NLRP3 inflammasome in primary murine macrophages infected by pseudorabies virus." Veterinary Microbiology 259, no. : 109130.
Streptococcus pneumoniae (S. pneumoniae) causes severe pulmonary diseases, leading to high morbidity and mortality. It has been reported that inflammasomes such as NLR family pyrin domain containing 3 (NLRP3) and absent in melanoma 2 (AIM2) play an important role in the host defense against S. pneumoniae infection. However, the role of NLRP6 in vivo and in vitro against S. pneumoniae remains unclear. Therefore, we investigated the role of NLRP6 in regulating the S. pneumoniae-induced inflammatory signaling pathway in vitro and the role of NLRP6 in the host defense against S. pneumoniae in vivo by using NLRP6−/− mice. The results showed that the NLRP6 inflammasome regulated the maturation and secretion of IL-1β, but it did not affect the induction of IL-1β transcription in S. pneumoniae-infected macrophages. Furthermore, the activation of caspase-1, caspase-11, and gasdermin D (GSDMD) as well as the oligomerization of apoptosis-associated speck-like protein (ASC) were also mediated by NLRP6 in S. pneumoniae-infected macrophages. However, the activation of NLRP6 reduced the expression of NF-κB and ERK signaling pathways in S. pneumoniae-infected macrophages. In vivo study showed that NLRP6−/− mice had a higher survival rate, lower number of bacteria, and milder inflammatory response in the lung compared with wild-type (WT) mice during S. pneumoniae infection, indicating that NLRP6 plays a negative role in the host defense against S. pneumoniae. Furthermore, increased bacterial clearance in NLRP6 deficient mice was modulated by the recruitment of macrophages and neutrophils. Our study provides a new insight on S. pneumoniae-induced activation of NLRP6 and suggests that blocking NLRP6 could be considered as a potential therapeutic strategy to treat S. pneumoniae infection.
Dongyi Xu; Xingping Wu; Lianci Peng; Tingting Chen; Qingyuan Huang; Yu Wang; Chao Ye; Yuanyi Peng; Dongliang Hu; Rendong Fang. The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo. International Journal of Molecular Sciences 2021, 22, 3876 .
AMA StyleDongyi Xu, Xingping Wu, Lianci Peng, Tingting Chen, Qingyuan Huang, Yu Wang, Chao Ye, Yuanyi Peng, Dongliang Hu, Rendong Fang. The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo. International Journal of Molecular Sciences. 2021; 22 (8):3876.
Chicago/Turabian StyleDongyi Xu; Xingping Wu; Lianci Peng; Tingting Chen; Qingyuan Huang; Yu Wang; Chao Ye; Yuanyi Peng; Dongliang Hu; Rendong Fang. 2021. "The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo." International Journal of Molecular Sciences 22, no. 8: 3876.
Staphylococcus aureus is a Gram-positive opportunistic pathogen which causes infections in a variety of vertebrates. Virulence factors are the main pathogenesis of S. aureus as a pathogen, which induce the host’s innate and adaptive immune responses. Toxic shock syndrome toxin 1 (TSST-1) is one of the most important virulence factors of S. aureus. However, the role of nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) in TSST-1-induced innate immune response is still unclear. Here, purified recombinant TSST-1 (rTSST-1) was prepared and used to stimulate mouse peritoneal macrophages. The results showed that under the action of adenosine-triphosphate (ATP), rTSST-1 significantly induced interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) production in mouse macrophages and the production was dose-dependent. In addition, rTSST-1+ATP-stimulated cytokine production in macrophage depends on the activation of toll like receptor 4 (TLR4), but not TLR2 on the cells. Furthermore, the macrophages of NLRP3−/− mice stimulated with rTSST-1+ATP showed significantly low levels of IL-1β production compared to that of wild-type mice. These results demonstrated that TSST-1 can induce the expression of inflammatory cytokines in macrophages via the activation of the TLR4 and NLRP3 signaling pathways. Our study provides new information about the mechanism of the TSST-1-inducing host’s innate immune responses.
Lianci Peng; Jiali Jiang; Tingting Chen; Dongyi Xu; Fengqing Hou; Qingyuan Huang; Yuanyi Peng; Chao Ye; Dong-Liang Hu; Rendong Fang. Toxic Shock Syndrome Toxin 1 Induces Immune Response via the Activation of NLRP3 Inflammasome. Toxins 2021, 13, 68 .
AMA StyleLianci Peng, Jiali Jiang, Tingting Chen, Dongyi Xu, Fengqing Hou, Qingyuan Huang, Yuanyi Peng, Chao Ye, Dong-Liang Hu, Rendong Fang. Toxic Shock Syndrome Toxin 1 Induces Immune Response via the Activation of NLRP3 Inflammasome. Toxins. 2021; 13 (1):68.
Chicago/Turabian StyleLianci Peng; Jiali Jiang; Tingting Chen; Dongyi Xu; Fengqing Hou; Qingyuan Huang; Yuanyi Peng; Chao Ye; Dong-Liang Hu; Rendong Fang. 2021. "Toxic Shock Syndrome Toxin 1 Induces Immune Response via the Activation of NLRP3 Inflammasome." Toxins 13, no. 1: 68.
Chao Ye; Xingping Wu; Tingting Chen; Qingyuan Huang; Rendong Fang; Tongqing An. The updated analysis of African swine fever virus genomes: Two novel genotypes are identified. Journal of Infection 2020, 80, 232 -254.
AMA StyleChao Ye, Xingping Wu, Tingting Chen, Qingyuan Huang, Rendong Fang, Tongqing An. The updated analysis of African swine fever virus genomes: Two novel genotypes are identified. Journal of Infection. 2020; 80 (2):232-254.
Chicago/Turabian StyleChao Ye; Xingping Wu; Tingting Chen; Qingyuan Huang; Rendong Fang; Tongqing An. 2020. "The updated analysis of African swine fever virus genomes: Two novel genotypes are identified." Journal of Infection 80, no. 2: 232-254.
Background Salmonella is one of the most important foodborne pathogens, causing outbreaks of human salmonellosis worldwide. Owing to large scales of consumption markets, pork and poultry that contaminated by Salmonella could pose a tremendous threat to public health. The aim of this study was to investigate the contamination of Salmonella from chicken, pork and the environment in slaughtering and retail processes in Chongqing, China. Results A total of 115 Salmonella isolates were recovered from 1112 samples collected from pork, chicken and the environment. Compared with the isolation rate of samples from chicken (9.50%) and the environment (6.23%), samples from pork had a significant higher isolation rate (44.00%). The isolation rates in slaughterhouses (10.76%) and in supermarkets (10.07%) showed no statistical difference. Thirty different serotypes were identified among all the isolates. S. Derby (n = 26), S. London (n = 16) and S. Rissen (n = 12) were the dominant serotypes. Antimicrobial susceptibility testing revealed that 73.04% isolates were resistant to tetracycline, followed by 66.96% to ampicillin and 59.13% to doxycycline. More than half (50.43%) of the isolates were multidrug resistant (MDR), and most of the MDR isolates were from supermarkets. Multilocus sequence typing results showed 24 out of 115 isolates were ST40, which was the most prevalent. Furthermore, isolates from supermarkets had 20 different sequence types while isolates from slaughterhouses only had 8 different sequence types. Conclusion Our study highlighted that Salmonella was more frequently isolated in pork production chain than that in chicken. Compared with isolates from slaughterhouses, isolates from supermarkets had more MDR profiles and represented a wider range of serotypes and sequence types, indicating that the retail process had more diverse sources of Salmonella contamination than that of slaughtering process.
Tingting Chen; Jiali Jiang; Chao Ye; Jianhua Xie; Xia Chen; Dongyi Xu; Zheng Zeng; Yuanyi Peng; Dong-Liang Hu; Rendong Fang. Genotypic characterization and antimicrobial resistance profile of Salmonella isolated from chicken, pork and the environment at abattoirs and supermarkets in Chongqing, China. BMC Veterinary Research 2019, 15, 1 -8.
AMA StyleTingting Chen, Jiali Jiang, Chao Ye, Jianhua Xie, Xia Chen, Dongyi Xu, Zheng Zeng, Yuanyi Peng, Dong-Liang Hu, Rendong Fang. Genotypic characterization and antimicrobial resistance profile of Salmonella isolated from chicken, pork and the environment at abattoirs and supermarkets in Chongqing, China. BMC Veterinary Research. 2019; 15 (1):1-8.
Chicago/Turabian StyleTingting Chen; Jiali Jiang; Chao Ye; Jianhua Xie; Xia Chen; Dongyi Xu; Zheng Zeng; Yuanyi Peng; Dong-Liang Hu; Rendong Fang. 2019. "Genotypic characterization and antimicrobial resistance profile of Salmonella isolated from chicken, pork and the environment at abattoirs and supermarkets in Chongqing, China." BMC Veterinary Research 15, no. 1: 1-8.
Corynebacterium pseudotuberculosis is a prominent human and animal pathogen causing chronic inflammatory diseases. Interleukin-1β (IL-1β) is involved in the response to such pathogenic infections. However, the mechanism by which IL-1β is secreted during C. pseudotuberculosis infection remains unclear. This study aimed to investigate the mechanism underlying IL-1β secretion by macrophages infected with C. pseudotuberculosis. Herein, we firstly revealed that nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC) and caspase-1 (Casp1) play critical roles in IL-1β secretion rather than IL-1β precursor (pro-IL-1β) expression in C. pseudotuberculosis-infected macrophages. Toll like receptor 4 (TLR4) is partially involved in IL-1β secretion, while absent in melanoma 2 (AIM2) is not involved in IL-1β secretion by C. pseudotuberculosis-infected macrophages. In addition, nuclear factor kappa B (NF-κB) and p38 mitogen-activated protein kinases (p38 MAPK) inhibitors almost attenuated IL-1β secretion, implying that NF-κB and p38MAPK pathway are involved in IL-1β secretion in C. pseudotuberculosis-infected macrophages. Furthermore, C. pseudotuberculosis were significantly more numerous in Nlrp3−/−, Asc−/−, and Casp-1−/− macrophages than in WT macrophages at 24 h after infection (P < 0.05), indicating that NLRP3 inflammasome components limit C. pseudotuberculosis replication in macrophages. Together, these data provide novel insights into the mechanisms underlying IL-1β secretion in C. pseudotuberculosis-infected macrophages and further the current understanding of the host pro-inflammatory immune response against this pathogen.
Zuoyong Zhou; Hexian Li; Shangquan Tian; Wenyi Yi; Yang Zhou; Haoyue Yang; Xiao Li; Bi Wu; Xiaoxia Li; Junjun Wu; Zhiying Wang; Shijun Hu; Rendong Fang. Critical roles of NLRP3 inflammasome in IL-1β secretion induced by Corynebacterium pseudotuberculosis in vitro. Molecular Immunology 2019, 116, 11 -17.
AMA StyleZuoyong Zhou, Hexian Li, Shangquan Tian, Wenyi Yi, Yang Zhou, Haoyue Yang, Xiao Li, Bi Wu, Xiaoxia Li, Junjun Wu, Zhiying Wang, Shijun Hu, Rendong Fang. Critical roles of NLRP3 inflammasome in IL-1β secretion induced by Corynebacterium pseudotuberculosis in vitro. Molecular Immunology. 2019; 116 ():11-17.
Chicago/Turabian StyleZuoyong Zhou; Hexian Li; Shangquan Tian; Wenyi Yi; Yang Zhou; Haoyue Yang; Xiao Li; Bi Wu; Xiaoxia Li; Junjun Wu; Zhiying Wang; Shijun Hu; Rendong Fang. 2019. "Critical roles of NLRP3 inflammasome in IL-1β secretion induced by Corynebacterium pseudotuberculosis in vitro." Molecular Immunology 116, no. : 11-17.
Streptococcus pneumoniae, a leading cause of invasive pneumococcal disease, is responsible for high mortality and morbidity worldwide. A previous study showed that the NLR family pyrin domain containing 3 (NLRP3) and absent in melanoma 2 (AIM2) inflammasomes are essential for caspase-1 activation and IL-1β production in the host response to S. pneumoniae infection. The function of NLRP3 in host innate immunity to S. pneumoniae was studied in vivo and in vitro. However, the role of AIM2 in host defence against S. pneumoniae remains unclear. Here, we show that AIM2-deficient (AIM2–/–) mice display increased susceptibility to intra-nasal infection with S. pneumoniae in comparison to wild type mice and that this susceptibility was associated with defective IL-1β production. Macrophages from AIM2–/– mice infected with S. pneumoniae showed impaired secretion of IL-1β as well as activation of the inflammasome, as determined by the oligomerisation of apoptosis-associated speck-like protein containing a CARD (ASC) and caspase-1 activation. Taken together, these results indicate that the AIM2 inflammasome is essential for caspase-1-dependent cytokine IL-1β production and eventual protection from pneumococcal infection in mice.
Siwei Feng; Tingting Chen; Guihua Lei; Fengqing Hou; Jiali Jiang; Qingyuan Huang; Yuanyi Peng; Chao Ye; Dong-Liang Hu; Rendong Fang. Absent in melanoma 2 inflammasome is required for host defence against Streptococcus pneumoniae infection. Innate Immunity 2019, 25, 412 -419.
AMA StyleSiwei Feng, Tingting Chen, Guihua Lei, Fengqing Hou, Jiali Jiang, Qingyuan Huang, Yuanyi Peng, Chao Ye, Dong-Liang Hu, Rendong Fang. Absent in melanoma 2 inflammasome is required for host defence against Streptococcus pneumoniae infection. Innate Immunity. 2019; 25 (7):412-419.
Chicago/Turabian StyleSiwei Feng; Tingting Chen; Guihua Lei; Fengqing Hou; Jiali Jiang; Qingyuan Huang; Yuanyi Peng; Chao Ye; Dong-Liang Hu; Rendong Fang. 2019. "Absent in melanoma 2 inflammasome is required for host defence against Streptococcus pneumoniae infection." Innate Immunity 25, no. 7: 412-419.
Streptococcus pneumoniae is a pathogenic bacterium that can cause severe invasive diseases, such as pneumonia, otitis media and meningitis. The pro-inflammatory cytokine, IL-1β, has been reported to play important role in host defense against S. pneumoniae. The mechanism of IL-1β maturation and secretion in macrophages has been well studied. However, the precise mechanism of IL-1β processing within neutrophils upon S. pneumoniae infection remains unclear. In this study, mouse peritoneal neutrophils from C57BL/6 WT and inflammasome components knockout mice were infected by S. pneumoniae in vitro. The results showed that NLRP3 inflammasome is critically involved in neutrophil IL-1β secretion, while the AIM2 and NLRC4 inflammasomes were dispensable. Moreover, the upstream kinase, JNK, modulates ASC oligomerization and consequent caspase-1 activation and IL-1β secretion. Additionally, neutrophil serine proteases also participate in IL-1β secretion by mediating ASC oligomerization and caspase-1 activation. Taken together, these findings indicated that both the NLRP3 inflammasome-related pathway and neutrophil serine protease mediate IL-1β processing upon S. pneumoniae infection.
Tingjuan Zhang; Huihui Du; Siwei Feng; Rui Wu; Tingting Chen; Jiali Jiang; Yuanyi Peng; Chao Ye; Rendong Fang. NLRP3/ASC/Caspase-1 axis and serine protease activity are involved in neutrophil IL-1β processing during Streptococcus pneumoniae infection. Biochemical and Biophysical Research Communications 2019, 513, 675 -680.
AMA StyleTingjuan Zhang, Huihui Du, Siwei Feng, Rui Wu, Tingting Chen, Jiali Jiang, Yuanyi Peng, Chao Ye, Rendong Fang. NLRP3/ASC/Caspase-1 axis and serine protease activity are involved in neutrophil IL-1β processing during Streptococcus pneumoniae infection. Biochemical and Biophysical Research Communications. 2019; 513 (3):675-680.
Chicago/Turabian StyleTingjuan Zhang; Huihui Du; Siwei Feng; Rui Wu; Tingting Chen; Jiali Jiang; Yuanyi Peng; Chao Ye; Rendong Fang. 2019. "NLRP3/ASC/Caspase-1 axis and serine protease activity are involved in neutrophil IL-1β processing during Streptococcus pneumoniae infection." Biochemical and Biophysical Research Communications 513, no. 3: 675-680.
Enzootic nasal adenocarcinoma (ENA) of goats, characterized by transformation of epithelial cells of the ethmoid turbinates, is caused by enzootic nasal tumor virus 2 (ENTV-2). ENTV-2 belongs to the genus Betaretrovirus and has extended its distribution globally with a high prevalence; however, the genetic diversity and genotypic distribution for ENTV-2 have not been analyzed systematically due to the limited availability of sequence data. In this study, an infection by ENTV-2 was detected by RT-PCR in Chongqing in July 2018, and the complete sequence of one strain (CQ1) was determined. Phylogenetic analysis indicated a high degree of genetic heterogeneity among ENTV-2 sequences, with the existence of two main lineages. Lineage 1 and 2 were composed of ENTV-2 from China and the UK, respectively. Although CQ1 was closely related to recent ENTV-2 strains collected in the neighboring provinces of Chongqing (Shaanxi and Sichuan), it formed a separate sublineage of lineage 1 (sublineage 1.3). This report will enhance our understanding of the epidemiology of ENTV-2 in China.
Chao Ye; Qingyuan Huang; Tingting Chen; Jiali Jiang; Fengqing Hou; Dongyi Xu; Yuanyi Peng; Rendong Fang; Jing Chen. First detection and genotypic analysis of goat enzootic nasal tumor virus 2 in Chongqing, China. Archives of Virology 2019, 164, 1647 -1650.
AMA StyleChao Ye, Qingyuan Huang, Tingting Chen, Jiali Jiang, Fengqing Hou, Dongyi Xu, Yuanyi Peng, Rendong Fang, Jing Chen. First detection and genotypic analysis of goat enzootic nasal tumor virus 2 in Chongqing, China. Archives of Virology. 2019; 164 (6):1647-1650.
Chicago/Turabian StyleChao Ye; Qingyuan Huang; Tingting Chen; Jiali Jiang; Fengqing Hou; Dongyi Xu; Yuanyi Peng; Rendong Fang; Jing Chen. 2019. "First detection and genotypic analysis of goat enzootic nasal tumor virus 2 in Chongqing, China." Archives of Virology 164, no. 6: 1647-1650.
Staphylococcus aureus is an important bacterial pathogen causing bovine mastitis, but little is known about the virulence factor and the inflammatory responses in the mammary infection. Staphylococcal enterotoxin C (SEC) is the most frequent toxin produced by S. aureus, isolated from bovine mastitis. To investigate the pathogenic activity of SEC in the inflammation of the mammary gland and the immune responses in an animal model, mouse mammary glands were injected with SEC, and the clinical signs, inflammatory cell infiltration, and proinflammatory cytokine production in the mammary glands were assessed. SEC induced significant inflammatory reactions in the mammary gland, in a dose-dependent manner. SEC-injected mammary glands showed a severe inflammation with inflammatory cell infiltration and tissue damage. In addition, interleukin (IL)-1β and IL-6 production in the SEC-injected mammary glands were significantly higher than those in the PBS control glands. Furthermore, the SEC-induced inflammation and tissue damage in the mammary gland were specifically inhibited by anti-SEC antibody. These results indicated, for the first time, that SEC can directly cause inflammation, proinflammatory cytokine production, and tissue damage in mammary glands, suggesting that SEC might play an important role in the development of mastitis associated with S. aureus infection. This finding offers an opportunity to develop novel treatment strategies for reduction of mammary tissue damage in mastitis.
Rendong Fang; Jingchun Cui; Tengteng Cui; Haiyong Guo; Hisaya K. Ono; Chun-Ho Park; Masashi Okamura; Akio Nakane; Dong-Liang Hu. Staphylococcal Enterotoxin C Is an Important Virulence Factor for Mastitis. Toxins 2019, 11, 141 .
AMA StyleRendong Fang, Jingchun Cui, Tengteng Cui, Haiyong Guo, Hisaya K. Ono, Chun-Ho Park, Masashi Okamura, Akio Nakane, Dong-Liang Hu. Staphylococcal Enterotoxin C Is an Important Virulence Factor for Mastitis. Toxins. 2019; 11 (3):141.
Chicago/Turabian StyleRendong Fang; Jingchun Cui; Tengteng Cui; Haiyong Guo; Hisaya K. Ono; Chun-Ho Park; Masashi Okamura; Akio Nakane; Dong-Liang Hu. 2019. "Staphylococcal Enterotoxin C Is an Important Virulence Factor for Mastitis." Toxins 11, no. 3: 141.
Enzootic nasal tumor virus 2 (ENTV-2), the aetiological agent of enzootic nasal adenocarcinoma in goats, is prevalent in China; resulting in substantial economic losses to the goat-breeding industry. Therefore, it is necessary to establish an efficient detection method for the diagnosis and prevention of ENTV-2 infection. More recently, EvaGreen is emerging as a novel alternative fluorescent dye for quantitative real-time PCR because of its low cost, specific amplification and high resolution. In this study, we developed a specific, sensitive, and cost-effective detection method-an EvaGreen-based real-time PCR assay for the detection of ENTV-2. This assay exhibited high specificity and sensitivity and was able to detect ENTV-2 at concentrations as low as 3.0 × 101 copies, which was more sensitive than the conventional PCR method (detection limit, 3.0 × 102 copies). In addition, the reproducibility test indicated that EvaGreen dye in our assay had a good reproducibility. In conclusion, we report that a highly sensitive, specific, and cost-effective EvaGreen-based real-time PCR assay is successful for the rapid detection of ENTV-2.
Qingyuan Huang; Chao Ye; Tingting Chen; Jiali Jiang; Yuanyi Peng; Jing Chen; Rendong Fang. EvaGreen-based real-time PCR assay for sensitive detection of enzootic nasal tumor virus 2. Molecular and Cellular Probes 2019, 44, 51 -56.
AMA StyleQingyuan Huang, Chao Ye, Tingting Chen, Jiali Jiang, Yuanyi Peng, Jing Chen, Rendong Fang. EvaGreen-based real-time PCR assay for sensitive detection of enzootic nasal tumor virus 2. Molecular and Cellular Probes. 2019; 44 ():51-56.
Chicago/Turabian StyleQingyuan Huang; Chao Ye; Tingting Chen; Jiali Jiang; Yuanyi Peng; Jing Chen; Rendong Fang. 2019. "EvaGreen-based real-time PCR assay for sensitive detection of enzootic nasal tumor virus 2." Molecular and Cellular Probes 44, no. : 51-56.
Streptococcus pneumoniae is a pathogen of significant clinical importance worldwide that can cause severe invasive diseases, such as pneumonia, otitis media and meningitis. Inflammsomes has been reported to participate in host defense against S. pneumoniae infection. S. pneumoniae could induce the assembly of the nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3)/absent in melanoma 2 (AIM2) inflammasome, which mediates the activation of caspase-1 and the subsequent maturation of Interleukin-1β (IL-1β). However, the precise signals that activate inflammasomes during pneumococcal infection remain to be fully elucidated. In the present study, primary mouse macrophages were selected as a cell model, and the effects of kinases on inflammasome activity induced by S. pneumoniae infection were examined by ELISA and western blotting after pretreatment with a kinase inhibitor. Here, we show that Syk and JNK signaling are required for S. pneumoniae-induced activation of the inflammasome. Inhibitors of Syk and JNK almost abolished the oligomerization of apoptosis-associated speck-like protein containing a caspase-activating and recruitment domain (ASC) and subsequent caspase-1 activation and IL-1β secretion. Moreover, pneumolysin (PLY) participated in this process and was critical for Syk/JNK activation. These results suggested that the Syk/JNK signaling pathway may play a vital role in the inflammasome activation and modulate host immune responses against S. pneumoniae.
Siwei Feng; Qingyuan Huang; Chao Ye; Rui Wu; Guihua Lei; Jiali Jiang; Tingting Chen; Yuanyi Peng; Rendong Fang. Syk and JNK signaling pathways are involved in inflammasome activation in macrophages infected with Streptococcus pneumoniae. Biochemical and Biophysical Research Communications 2018, 507, 217 -222.
AMA StyleSiwei Feng, Qingyuan Huang, Chao Ye, Rui Wu, Guihua Lei, Jiali Jiang, Tingting Chen, Yuanyi Peng, Rendong Fang. Syk and JNK signaling pathways are involved in inflammasome activation in macrophages infected with Streptococcus pneumoniae. Biochemical and Biophysical Research Communications. 2018; 507 (1-4):217-222.
Chicago/Turabian StyleSiwei Feng; Qingyuan Huang; Chao Ye; Rui Wu; Guihua Lei; Jiali Jiang; Tingting Chen; Yuanyi Peng; Rendong Fang. 2018. "Syk and JNK signaling pathways are involved in inflammasome activation in macrophages infected with Streptococcus pneumoniae." Biochemical and Biophysical Research Communications 507, no. 1-4: 217-222.
The Pasteurella multocida capsular type A isolates can cause pneumonia and bovine respiratory disease (BRD). In this study, comparative genomics analysis was carried out to identify the virulence genes in two different virulent P. multocida capsular type A isolates (high virulent PmCQ2 and low virulent PmCQ6). The draft genome sequence of PmCQ2 is 2.32 Mbp and contains 2,002 protein-coding genes, 9 insertion sequence (IS) elements, and 1 prophage region. The draft genome sequence of PmCQ6 is 2.29 Mbp and contains 1,970 protein-coding genes, 2 IS elements, and 3 prophage regions. The genome alignment analysis revealed that the genome similarity between PmCQ2 and PmCQ6 is 99% with high colinearity. To identify the candidate genes responsible for virulence, the PmCQ2 and PmCQ6 were compared together with that of the published genomes of high virulent Pm36950 and PmHN06 and avirulent Pm3480 and Pm70 (capsular type F). Five genes and two insertion sequences are identified in high virulent strains but not in low virulent or avirulent strains. These results indicated that these genes or insertion sequences might be responsible for the virulence of P. multocida, providing prospective candidates for further studies on the pathogenesis and the host-pathogen interactions of P. multocida.
Huihui Du; Rendong Fang; Tingting Pan; Tian Li; Nengzhang Li; Qiang He; Rui Wu; Yuanyi Peng; Zeyang Zhou. Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates. International Journal of Genomics 2016, 2016, 1 -14.
AMA StyleHuihui Du, Rendong Fang, Tingting Pan, Tian Li, Nengzhang Li, Qiang He, Rui Wu, Yuanyi Peng, Zeyang Zhou. Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates. International Journal of Genomics. 2016; 2016 ():1-14.
Chicago/Turabian StyleHuihui Du; Rendong Fang; Tingting Pan; Tian Li; Nengzhang Li; Qiang He; Rui Wu; Yuanyi Peng; Zeyang Zhou. 2016. "Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates." International Journal of Genomics 2016, no. : 1-14.