While nanoparticles (NPs) can be used as insecticides by themselves, they can also be carriers for insecticidal chemicals. Existing literature suggests that the smaller the NP size, the greater the toxicity and penetration into the insect's body. Nonetheless, there is a lack of literature pertaining to the mode of action within insects. This review article summarizes the currently available entomological studies on the mechanisms of NP-insect interactions. Externally, NPs affect pigmentation and integrity of the cuticle, while internally they induce immune responses and alter gene expression leading to altered protein, lipid, and carbohydrate metabolism along with cellular toxicity that impairs development and reproduction of the insect. Consequently, insects are incapacitated due to the disruption of the nutrient intake, production of reactive oxygen species and altered biochemical activity while some NPs can promote growth and development as well as diminish the effects of nontarget toxicity.

In the present in vivo study, we provide a comparison of toxicological consequences induced by four different types of spherical nanoparticles (NPs)—silver nanoparticles (AgNPs, 40 ± 6 nm), nickel (NiNPs, 43 ± 6 nm), cobalt oxide (Co₃O₄NPs, 60 ± 6 nm), and chromium oxide (Cr₃O₄NPs, 50 ± 5 nm)—on freshwater fish Labeo rohita. Fish were exposed to NPs (25 mg/L) for 21 days. We observed a NPs type-dependent toxicity in fish. An altered behavior showing signs of stress and a substantial reduction in total leukocyte count was noticed in all NP-treated groups. A low total erythrocyte count in all NP-treated fish except for Co₃O₄NPs was discerned while a low survival rate in the case of Cr₃O₄NP-treated fish was observed. A significant decrease in growth and hemoglobin were noticed in NiNP- and Cr₃O₄NP-treated fish. A considerable total protein elevation was detected in NiNP-, Co₃O₄NP-, and Cr₃O₄NP-treated groups. An upgrading in albumin level was witnessed in Co₃O₄NP- and Cr₃O₄NP-treated groups while a high level of globulin was noted in NiNP- and Co₃O₄NP-exposed groups. In all NP-treated groups, a depleted activity of antioxidative enzymes and pathological lesions in liver and kidney were noticed.

This paper presents the results of radon (²²²Rn) concentration measurements in municipal supply drinking water in metropolitan Lahore city of Pakistan and evaluation of consequent radiological effects. In this respect, water samples were collected in all nine municipal towns of Lahore city and analysed employing a high-resolution gamma spectrometric system. Radon concentration varied from 2.0 ± 0.3 to 7.9 ± 2.1 Bq l⁻¹. Mean value of annual effective dose for an individual consumer was assessed to be 16.5 ± 12.8 µSv y⁻¹. ²²²Rn mean concentration measured in this study is comparable with the reported values for drinking water determined worldwide and found to be less than the limit of 100 Bq l⁻¹ recommended by the World Health Organisation for public water supplies. The results of this study may be helpful in establishing background levels of radon in drinking water that could be used not only to distinguish additional contributions when a contamination event occurs but also to implement water quality standards by the concerned authorities to maintain radioactive contamination free drinking water supplies for the population.

The toll of workplace bullying is immense, yet, similarly as with an iceberg, its scope, scale and implications tend to remain underestimated. Several ways of assessing the prevalence of workplace bullying have been proposed in the literature. The most frequently discussed are the ‘subjective method’ assessing individuals’ perceptions of being a victim and the questionnaire, i.e., criterion-based, methods, including Negative Acts Questionnaire (NAQ) and Leymann Inventory of Psychological Terror (LIPT). Since in both cases culture plays a profound role as a mediating factor in the process of identifying, collecting, and processing data, the applicability of these methods across cultures and countries has several limitations. At this stage, it is impossible to determine the impact of the implicit cultural-bias that these methods entail on the research outcomes. This would be possible if an alternative workplace bullying scale (WBS) was at hand and, consequently, a comparative analysis was conducted. This paper, drawing from a study conducted at higher education institutions (HEI) across Pakistan, addresses this issue by devising an alternative WBS. The value added of this paper is three-fold, i.e., it elaborates on the study and the specific methods employed to prove the validity and relevance of the alternative WBS. Moreover, by so doing, it addresses some of the limitations that other methods measuring the prevalence of workplace bullying display. As a result, it adds to the researchers’ and administrators’ toolkit as regards research and policies aimed at mitigating the scope and scale of bullying at HEIs across cultures and countries.

This study focuses on isolation of pathogenic bacteria from external and internal surfaces of cockroaches collected from houses and hospitals in Lahore. In total, 240 adult cockroaches were collected from houses and hospitals by hand or using sticky traps and food-bait traps. Cockroach species were identified, and microbial screening was done for external surfaces and gut tracts of cockroaches. Jaccard’s index of similarity, Bray–Curtis’ index of dissimilarity, and Shannon–Wiener’s diversity index were used to measure the bacterial community diversity (all species of bacteria) in each habitat. Relative abundance and frequency were measured for each bacterial species on external and internal surfaces of cockroaches. Among human habitations, two major species of cockroaches were isolated, i.e., Periplaneta americana ( P . americana ) and Blattella germanica ( B . germanica ). Out of 240 cockroaches collected, 167 were P . americana and 73 were B . germanica . In total, 11 bacterial species were isolated, but no different bacterial load in each habitat was observed. The most common diagnostic bacterium isolated from the external surface of cockroaches was found to be Escherichia coli (10.31%). In contrast, the most common isolate from the internal gut tract of cockroaches was found to be Pseudomonas aeruginosa , with relative frequency of 19.96%. Jaccard’s index of similarity of bacterial species found on cockroaches was highest (0.3125) in houses, whereas Bray–Curtis’ index of dissimilarity was highest for hospitals (0.2174). The highest Shannon–Wiener’s diversity index value was found in bacteria on cockroaches collected from the Punjab Institute of Cardiology (2.610632). No Salmonella typhi , Staphylococcus epidermidis , Enterobacter aerogenes , and Streptococcus pneumonia were found in the digestive tract of any cockroach.