As a gas signal molecule, hydrogen sulfide (H₂S) can enhance plant stress resistance. Here, cucumber (Cucumis sativus ‘Xinchun NO. 4’) explants were used to investigate the role of H₂S in adventitious root development under salt stress. The results show that sodium chloride (NaCl) at 10 mM produced moderate salt stress. The 100 µM sodium hydrosulfide (NaHS) treatment, a H₂S donor, increased root number and root length by 38.37% and 66.75%, respectively, indicating that H₂S effectively promoted the occurrence of adventitious roots in cucumber explants under salt stress. The results show that under salt stress, NaHS treatment reduced free proline content and increased the soluble sugar and soluble protein content during rooting. Meanwhile, NaHS treatment enhanced the activities of antioxidant enzymes [peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)], increased the content of ascorbic (ASA) and glutathione (GSH), reduced the content of hydrogen peroxide (H₂O₂) and the rate of superoxide radical (O²⁻) production, and decreased relative electrical conductivity (REC) and the content of malondialdehyde (MDA). However, the NaHS scavenger hypotaurine (HT) reversed the above effects of NaHS under salt stress. In summary, H₂S promoted adventitious root development under salt stress through regulating osmotic substance content and enhancing antioxidant ability in explants.