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To support the introduction of local soft wheat varieties, the Lebanese state has implemented, during recent years, an improvement program to select plant material with good productivity in semi-arid conditions that can lead to national production that can, at the same time, meet quality needs expressed by Lebanese processors. In the present study, the main biochemical components of grains conventionally associated with bread-making properties (storage proteins, starch polysaccharides) were physiochemically characterized for a panel of local soft wheat varieties selected. During the two consecutive years of cultivation, the post-flowering thermal constraints significantly modified the kinetics of accumulation of the major constituents by mainly limiting the grain filling time. The level of polymerization/aggregation (i.e., gliadin/glutenin ratio, unextractable polymeric protein (UPP) amount, molecular weight average of glutenins) of prolamins was very high. The reduction in the amount of starch was accompanied by a significant change in the amylopectin/amylose ratio. Finally, the genotypes studied were characterized by significantly different distributions of starch granules; the percentage of the volume occupied by A-type and B-type starch granules varied between genotypes for the two cropping years. All these observations must be considered because of their determining role in the technological aptitude of the flours generated.
Nada Sakr; Larbi Rhazi; Thierry Aussenac. Bread Wheat Quality under Limiting Environmental Conditions: I-Molecular Properties of Storage Proteins and Starch Constituents in Mature Grains. Agriculture 2021, 11, 289 .
AMA StyleNada Sakr, Larbi Rhazi, Thierry Aussenac. Bread Wheat Quality under Limiting Environmental Conditions: I-Molecular Properties of Storage Proteins and Starch Constituents in Mature Grains. Agriculture. 2021; 11 (4):289.
Chicago/Turabian StyleNada Sakr; Larbi Rhazi; Thierry Aussenac. 2021. "Bread Wheat Quality under Limiting Environmental Conditions: I-Molecular Properties of Storage Proteins and Starch Constituents in Mature Grains." Agriculture 11, no. 4: 289.
Ozone is recognized as an antimicrobial agent for vegetables storage, washing, and processing. This strong disinfectant is now being used in the food industry. In this review, the chemical and physical properties of ozone, its generation, and factors affecting ozone processing efficiency were explained as well as recent regulatory developments in the food industry. By then selecting three vegetables, we show that ozone avoids and controls biological growth on vegetables, keeping their attractive appearance and sensorial qualities, assuring nutritional characteristics’ retention and maintaining and increasing the shelf-life. In liquid solution, ozone can be used to disinfect processing water and vegetables, and in gaseous form, ozone helps to sanitize and preserve vegetables during storage. The multifunctionality of ozone makes it a promising food processing agent. However, if ozone is improperly used, it causes some deleterious effects on products, such as losses in their sensory quality. For an effective and a safe use of ozone, specific treatment conditions should be determined for all kinds of vegetables. In a last step, we propose highlighting the different essential characteristics of ozone treatment in order to internationally harmonize the data relating to the treatments carried-out.
Elodie Sarron; Pascale Gadonna-Widehem; Thierry Aussenac. Ozone Treatments for Preserving Fresh Vegetables Quality: A Critical Review. Foods 2021, 10, 605 .
AMA StyleElodie Sarron, Pascale Gadonna-Widehem, Thierry Aussenac. Ozone Treatments for Preserving Fresh Vegetables Quality: A Critical Review. Foods. 2021; 10 (3):605.
Chicago/Turabian StyleElodie Sarron; Pascale Gadonna-Widehem; Thierry Aussenac. 2021. "Ozone Treatments for Preserving Fresh Vegetables Quality: A Critical Review." Foods 10, no. 3: 605.
In the general framework of a program to improve the local production of common wheat by the Lebanese State, we have characterized the rheology of different bread wheat cultivars selected for their adaptation to semi-arid weather conditions. After a biochemical characterization [i.e. synthesis and accumulation of the major constituents of the grains (mainly storage proteins and starch)] of the plant material selected in a previous work, the main technological behavior of the flour that resulted from these grains and associated with bread-making properties was evaluated in the present study. Despite the selection of the plant material used in this study, the results demonstrate that environmental conditions inducing limitations in reserve accumulation [i.e. high gliadin content, very high level of polymerization/aggregation of polymeric prolamins, significant increase in the amylopectin/amylose ratio, modifications in the distribution of starch granules (A-type vs. B-type)] during grain filling affected the rheological behavior. The studied flours showed high wet and dry gluten contents, high Zeleny values, and a low gluten index value. They also showed high extensibility on the alveograph and extensograph and elevated dough strength on the farinograph, as well as great water absorption. Furthermore, flours of the studied cultivars did not express their optimal rheological potential in spite of their favorable glutenin subunit composition.
Nada Sakr; Larbi Rhazi; Thierry Aussenac. Bread wheat quality under limiting environmental conditions: II – Rheological properties of Lebanese wheat genotypes. Journal of the Saudi Society of Agricultural Sciences 2021, 20, 235 -242.
AMA StyleNada Sakr, Larbi Rhazi, Thierry Aussenac. Bread wheat quality under limiting environmental conditions: II – Rheological properties of Lebanese wheat genotypes. Journal of the Saudi Society of Agricultural Sciences. 2021; 20 (4):235-242.
Chicago/Turabian StyleNada Sakr; Larbi Rhazi; Thierry Aussenac. 2021. "Bread wheat quality under limiting environmental conditions: II – Rheological properties of Lebanese wheat genotypes." Journal of the Saudi Society of Agricultural Sciences 20, no. 4: 235-242.
Research approaches on the use of ecotechnologies like ozone assisted processes for the decolorization of textiles are being explored as against the conventional alkaline reductive process for the color stripping of the cotton textiles. The evaluation of these ecotechnologies must be performed to assess the environmental impacts. Partial “gate to gate” Life Cycle Assessment (LCA) was implemented to study the ozone based decolorization process of the reactive dyed cotton textiles. Experiments were performed to determine input and output data flows for decolorization treatment of reactive dyed cotton textile using the ozonation process. The functional unit was defined as “treatment of 40 g of reactive dyed cotton fabric to achieve more than 94% color stripping”. Generic and specific data bases were also used to determine flows, and International Life Cycle Data system (ILCD) method was selected to convert all flows into environmental impacts. The impact category “Water resource depletion” is the highest for all the ozonation processes as it has the greatest relative value after normalization amongst all the impact indicators. Electricity and Oxygen formation were found to be the major contributors to the environmental impacts. New experimental conditions have been studied to optimize the impacts.
Ajinkya Powar; Anne Perwuelz; Nemeshwaree Behary; Le Hoang; Aussenac Thierry; Carmen Loghin; Stelian Maier; Jinping Guan; Guoqiang Chen. Environmental Profile Study of Ozone Decolorization of Reactive Dyed Cotton Textiles by Utilizing Life Cycle Assessment. Sustainability 2021, 13, 1225 .
AMA StyleAjinkya Powar, Anne Perwuelz, Nemeshwaree Behary, Le Hoang, Aussenac Thierry, Carmen Loghin, Stelian Maier, Jinping Guan, Guoqiang Chen. Environmental Profile Study of Ozone Decolorization of Reactive Dyed Cotton Textiles by Utilizing Life Cycle Assessment. Sustainability. 2021; 13 (3):1225.
Chicago/Turabian StyleAjinkya Powar; Anne Perwuelz; Nemeshwaree Behary; Le Hoang; Aussenac Thierry; Carmen Loghin; Stelian Maier; Jinping Guan; Guoqiang Chen. 2021. "Environmental Profile Study of Ozone Decolorization of Reactive Dyed Cotton Textiles by Utilizing Life Cycle Assessment." Sustainability 13, no. 3: 1225.
This study investigates genetic and environmental variation in starch content and characteristics of 14 French bread cultivars. Understanding the impact of these factors on wheat quality is important for processors and especially bakers to maintain and meet the requirements of industrial specifications. Different traits were evaluated: starch content, distribution of starch granules, percentage of amylose and amylopectin and their molecular characteristics (weight-average molar mass, number-average molar mass, polydispersity and gyration radius). Genetic, environment and their interaction had significant effects on all parameters. The relative magnitude of variance attributed to growth conditions, for most traits, was substantially higher (21% to 95%) than that attributed to either genotype (2% to 73%) or G × E interaction (2% to 17%). The largest environmental contribution (95%) to total variance was found for starch dispersity. The highest genetic influence was found for the percentage of A-type starch granules. G × E interaction had relatively little influence (≈7%) on total phenotypic variance. All molecular characteristics were much more influenced by environment than the respective percentages of amylose and amylopectin were. This huge difference in variance between factors obviously revealed the importance of the effect of growing conditions on characteristics of cultivars.
Larbi Rhazi; Benoît Méléard; Olfa Daaloul; Guénolé Grignon; Gérard Branlard; Thierry Aussenac. Genetic and Environmental Variation in Starch Content, Starch Granule Distribution and Starch Polymer Molecular Characteristics of French Bread Wheat. Foods 2021, 10, 205 .
AMA StyleLarbi Rhazi, Benoît Méléard, Olfa Daaloul, Guénolé Grignon, Gérard Branlard, Thierry Aussenac. Genetic and Environmental Variation in Starch Content, Starch Granule Distribution and Starch Polymer Molecular Characteristics of French Bread Wheat. Foods. 2021; 10 (2):205.
Chicago/Turabian StyleLarbi Rhazi; Benoît Méléard; Olfa Daaloul; Guénolé Grignon; Gérard Branlard; Thierry Aussenac. 2021. "Genetic and Environmental Variation in Starch Content, Starch Granule Distribution and Starch Polymer Molecular Characteristics of French Bread Wheat." Foods 10, no. 2: 205.
We characterized the molecular weight distribution of polymeric proteins (PP) of bread wheat grains using asymmetric flow field flow fractionation (A4F). The experiment, involving six environmental conditions and 130 cultivars, offered the opportunity to approach the phenotypic values of the polymer characteristics and their contribution of the rheological properties of flours and/or doughs. The contents of high-molecular-weight polymers (MW > 2 × 106 g·mol−1) that can be considered as “rheologically active polymers” (RAPP) for their major contribution to dough baking strength and mixing tolerance were mainly controlled by environmental factors. Under the influence of the growing conditions, at the cellular level, the redox status of non-protein free thiol, such as glutathione, is modified and leads to the formation of polymeric protein-bound glutathione conjugates (PPSSG). The accumulation of these conjugates reduces the formation of the RAPP by limiting the intermolecular interactions between PP in the grain during desiccation. This phenomenon is, therefore, potentially responsible for decreases in the technological properties of the wheat genotypes concerned. These first results invite us to continue our investigations to fully confirm this phenomenon, with emphasis on the behavior of wheat genotypes under various growing conditions.
Thierry Aussenac; Larbi Rhazi; Gérard Branlard. Molecular Weight Distribution of Polymeric Proteins in Wheat Grains: The Rheologically Active Polymers. Foods 2020, 9, 1675 .
AMA StyleThierry Aussenac, Larbi Rhazi, Gérard Branlard. Molecular Weight Distribution of Polymeric Proteins in Wheat Grains: The Rheologically Active Polymers. Foods. 2020; 9 (11):1675.
Chicago/Turabian StyleThierry Aussenac; Larbi Rhazi; Gérard Branlard. 2020. "Molecular Weight Distribution of Polymeric Proteins in Wheat Grains: The Rheologically Active Polymers." Foods 9, no. 11: 1675.
The polymers of wheat glutenins are studied here using asymmetric flow field flow fractionation (A4F). Molecular mass (Mw), gyration radius (Rw), and the polydispersity index (PI) of polymers were measured over a four-year, multi-local wheat trial in France. The experiment, involving 11 locations and 192 cultivars, offered the opportunity to approach the genetic and environmental factors associated with the phenotypic values of the polymer characteristics. These characteristics, which were all highly influenced by environmental factors, exhibited low broad-sense heritability coefficients and were not influenced by grain protein content and grain hardness. The 31 alleles encoding the glutenin subunits explained only 17.1, 25.4, and 16.8% of the phenotypic values of Mw, Rw, and PI, respectively. The climatic data revealed that a 3.5 °C increase between locations of the daily average temperature, during the last month of the grain development, caused an increase of more than 189%, 242%, and 434% of the Mw, Rw, and PI, respectively. These findings have to be considered in regard to possible consequences of global warming and health concerns assigned to gluten. It is suggested that the molecular characteristics of glutenins be measured today, especially for research addressing non-celiac gluten sensitivity (NCGS).
Gérard Branlard; Annie Faye; Larbi Rhazi; Ayesha Tahir; Véronique Lesage; Thierry Aussenac. Genetic and Environmental Factors Associated to Glutenin Polymer Characteristics of Wheat. Foods 2020, 9, 683 .
AMA StyleGérard Branlard, Annie Faye, Larbi Rhazi, Ayesha Tahir, Véronique Lesage, Thierry Aussenac. Genetic and Environmental Factors Associated to Glutenin Polymer Characteristics of Wheat. Foods. 2020; 9 (5):683.
Chicago/Turabian StyleGérard Branlard; Annie Faye; Larbi Rhazi; Ayesha Tahir; Véronique Lesage; Thierry Aussenac. 2020. "Genetic and Environmental Factors Associated to Glutenin Polymer Characteristics of Wheat." Foods 9, no. 5: 683.
The decolorization of a cotton fabric dyed with a reactive dye (C.I. Reactive Black 5) was studied using an optimized ozone-assisted process at pilot scale. Box–Behnken design was used to evaluate the effects of three parameters on the decolorization of the dyed textile, namely, pH of the treatment (3–7), ozone concentration (5–85 g/m3 of ozone), and treatment time (10–50 min). The fitted mathematical model allowed us to plot response surfaces as well as isoresponse curves and to determine optimal decolorization conditions. In this study, we have proposed a pilot-scale machine which utilizes ozone for the color stripping of the dyed cotton. This pilot-scale application opens up the route for application of ozone at an industrial scale for achieving sustainability in the textile industry.
Ajinkya Sudhir Powar; Anne Perwuelz; Nemeshwaree Behary; Levinh Hoang; Thierry Aussenac. Application of Ozone Treatment for the Decolorization of the Reactive-Dyed Fabrics in a Pilot-Scale Process—Optimization through Response Surface Methodology. Sustainability 2020, 12, 471 .
AMA StyleAjinkya Sudhir Powar, Anne Perwuelz, Nemeshwaree Behary, Levinh Hoang, Thierry Aussenac. Application of Ozone Treatment for the Decolorization of the Reactive-Dyed Fabrics in a Pilot-Scale Process—Optimization through Response Surface Methodology. Sustainability. 2020; 12 (2):471.
Chicago/Turabian StyleAjinkya Sudhir Powar; Anne Perwuelz; Nemeshwaree Behary; Levinh Hoang; Thierry Aussenac. 2020. "Application of Ozone Treatment for the Decolorization of the Reactive-Dyed Fabrics in a Pilot-Scale Process—Optimization through Response Surface Methodology." Sustainability 12, no. 2: 471.
Plant-growth-promoting rhizobacteria are known as potential biofertilizers and plant-resistance inducers. The current work aims to study the durability of the resistance induced as a response to the inoculation of wheat grains with Paenibacillus sp. strain B2 (PB2) and its influence by plant genotype, growth stage, and Mycosphaerella graminicola strain (the causal agent of Septoria tritici blotch or STB). The results of the plate-counting method showed that PB2 has high potential for wheat-root external colonization [>106 colony-forming unit (CFU)/g of root], and the quantitative real-time polymerase chain reaction (qPCR) analysis demonstrated its internal root-colonization capacity on all tested cultivars. However, the colonization seems to be dependent on wheat-growth stage. The durability of PB2-induced resistance (PB2-IR) was tested at the 3-leaf, tillering, and flag-leaf-growth stages. Additionally, the results showed that the PB2-IR is durable and able to protect the flag leaf, the most important leaf layer during grain fill. It conferred a high protection efficiency (55–94%) against four virulent strains of M. graminicola and over 11 wheat cultivars with different resistance levels to STB. Although, PB2-IR is dependent on M. graminicola strains, wheat genotypes and growth stages, its efficiency, under field conditions, at protecting the last wheat-leaf layers was not an influence. However, it showed 71–79% of protection and reached 81–94% in association with half of the recommended dose of Cherokee® fungicide. This may be explained using laboratory results by its direct impact on M. graminicola strains in these leaf layers and by the indirect reduction of the inoculum coming from leaves infected during the earlier growth stages. Gene expression results showed that PB2-IR is correlated to upregulation of genes involved in defense and cell rescue and a priming effect in the basal defense, jasmonic acid signaling, phenylpropanoids and phytoalexins, and reactive oxygen species gene markers. To conclude, PB2 induces a high and durable resistance against M. graminicola under controlled and field conditions. The PB2-IR is a pathogen strain and is plant-growth-stage and genotype dependent. These results highlight the importance of taking into consideration these factors so as to avoid losing the effectiveness of induced resistance under field conditions.
Erika Samain; Thierry Aussenac; Sameh Selim. The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2. Frontiers in Plant Science 2019, 10, 587 .
AMA StyleErika Samain, Thierry Aussenac, Sameh Selim. The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2. Frontiers in Plant Science. 2019; 10 ():587.
Chicago/Turabian StyleErika Samain; Thierry Aussenac; Sameh Selim. 2019. "The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2." Frontiers in Plant Science 10, no. : 587.
Aussenac Thierry; Rhazi Larbi. Storage Proteins Accumulation and Aggregation in Developing Wheat Grains. Global Wheat Production 2018, 1 .
AMA StyleAussenac Thierry, Rhazi Larbi. Storage Proteins Accumulation and Aggregation in Developing Wheat Grains. Global Wheat Production. 2018; ():1.
Chicago/Turabian StyleAussenac Thierry; Rhazi Larbi. 2018. "Storage Proteins Accumulation and Aggregation in Developing Wheat Grains." Global Wheat Production , no. : 1.
Many of the non-pathogenic endophytic bacteria that reside in plant roots promote plant growth as well as protection against pathogens attack. However, little is known about their mode of action in wheat. We have previously demonstrated the potential of Paenibacillus sp. strain B2 (PB2) to stimulate plant defense mechanisms via its paenimyxin lipo-polypeptide elicitor. Recently, we isolated the Curtobacterium plantarum strain EDS (EDS) from seeds of almost all wheat cultivars. In the present work, the ability of PB2 and EDS to promote wheat growth and protection against Septoria leaf blotch (SLB) was investigated. Results showed that PB2 is a general root external colonizer and cultivar-dependent endophyte. In the endophytic state and only in co-inoculation, it significantly increased the internal root colonization by EDS, resulting in an increase of root and aerial part fresh weights. qPCR analysis showed that, in the endophytic and nonendophytic states, PB2 conferred >= 59% protection against SLB by inducing systemic resistance which is characterized by the over expression of the pr1, lox, Aos, peroxidase, oxo and gst genes. Paenimyxin conferred 76% local protection characterized by the overexpression of the glu, lox, aos, pal, chs, oxo, and gst genes, and 82% systemic protection by chs. It was concluded that PB2 is potentially very interesting in the biocontrol of SLB and, in a mixture with EDS, in the wheat growth promoting. Genes involved in the flavonoid, salicylic acid, jasmonic acid, reactive oxygen species and basal defense pathways seem to play an important role in the resistance against SLB
Erika Samain; Diederik van Tuinen; Philippe Jeandet; Thierry Aussenac; Sameh Selim. Biological control of septoria leaf blotch and growth promotion in wheat by Paenibacillus sp. strain B2 and Curtobacterium plantarum strain EDS. Biological Control 2017, 114, 87 -96.
AMA StyleErika Samain, Diederik van Tuinen, Philippe Jeandet, Thierry Aussenac, Sameh Selim. Biological control of septoria leaf blotch and growth promotion in wheat by Paenibacillus sp. strain B2 and Curtobacterium plantarum strain EDS. Biological Control. 2017; 114 ():87-96.
Chicago/Turabian StyleErika Samain; Diederik van Tuinen; Philippe Jeandet; Thierry Aussenac; Sameh Selim. 2017. "Biological control of septoria leaf blotch and growth promotion in wheat by Paenibacillus sp. strain B2 and Curtobacterium plantarum strain EDS." Biological Control 114, no. : 87-96.
Perrine Gozé; Larbi Rhazi; Lyès Lakhal; Philippe Jacolot; André Pauss; Thierry Aussenac. Effects of ozone treatment on the molecular properties of wheat grain proteins. Journal of Cereal Science 2017, 75, 243 -251.
AMA StylePerrine Gozé, Larbi Rhazi, Lyès Lakhal, Philippe Jacolot, André Pauss, Thierry Aussenac. Effects of ozone treatment on the molecular properties of wheat grain proteins. Journal of Cereal Science. 2017; 75 ():243-251.
Chicago/Turabian StylePerrine Gozé; Larbi Rhazi; Lyès Lakhal; Philippe Jacolot; André Pauss; Thierry Aussenac. 2017. "Effects of ozone treatment on the molecular properties of wheat grain proteins." Journal of Cereal Science 75, no. : 243-251.
Soft wheat grains were subjected to oxidation by ozone treatment. A Box Benhken design with three variables (humidification rate, ozone inlet concentration and reaction time) was used for the experiment. Two wheat cultivars differing on their technological properties were used. The products were characterized by determining rheological, thermal and other physicochemical properties such as RVA (Rapid Visco Analyzer), DSC (Differential Scanning Calorimetry), molecular weight distribution and amylopectin branched chain length distribution. Contrary to previous works, results clearly show no significant effect of ozone treatment on wheat starch in our experimental conditions (i.e. starch extracted from ozonated whole wheat grain) whatever the cultivar studied. Only slight increases of carboxyl groups have been noticed with increasing ozonation. Modifications observed on rheological properties in flour samples (i.e. alveographic and RVA measurements) in previous works (Gozé et al., 2015) could mainly be explained by the oxidation of others molecules such as proteins and non-starch polysaccharides.
Perrine Gozé; Larbi Rhazi; André Pauss; Thierry Aussenac. Starch characterization after ozone treatment of wheat grains. Journal of Cereal Science 2016, 70, 207 -213.
AMA StylePerrine Gozé, Larbi Rhazi, André Pauss, Thierry Aussenac. Starch characterization after ozone treatment of wheat grains. Journal of Cereal Science. 2016; 70 ():207-213.
Chicago/Turabian StylePerrine Gozé; Larbi Rhazi; André Pauss; Thierry Aussenac. 2016. "Starch characterization after ozone treatment of wheat grains." Journal of Cereal Science 70, no. : 207-213.
Olfa Daaloul Bouacha; Larbi Rhazi; Thierry Aussenac; Salah Rezgui; Sadok Nouaigui. Molecular characterization of storage proteins for selected durum wheat varieties grown in different environments. Journal of Cereal Science 2015, 61, 97 -104.
AMA StyleOlfa Daaloul Bouacha, Larbi Rhazi, Thierry Aussenac, Salah Rezgui, Sadok Nouaigui. Molecular characterization of storage proteins for selected durum wheat varieties grown in different environments. Journal of Cereal Science. 2015; 61 ():97-104.
Chicago/Turabian StyleOlfa Daaloul Bouacha; Larbi Rhazi; Thierry Aussenac; Salah Rezgui; Sadok Nouaigui. 2015. "Molecular characterization of storage proteins for selected durum wheat varieties grown in different environments." Journal of Cereal Science 61, no. : 97-104.
Larbi Rhazi; Robert A. Graybosch; Thierry Aussenac. Molecular characterization of three transgenic high molecular weight glutenin subunit events in winter wheat. Journal of Cereal Science 2014, 60, 631 -638.
AMA StyleLarbi Rhazi, Robert A. Graybosch, Thierry Aussenac. Molecular characterization of three transgenic high molecular weight glutenin subunit events in winter wheat. Journal of Cereal Science. 2014; 60 (3):631-638.
Chicago/Turabian StyleLarbi Rhazi; Robert A. Graybosch; Thierry Aussenac. 2014. "Molecular characterization of three transgenic high molecular weight glutenin subunit events in winter wheat." Journal of Cereal Science 60, no. 3: 631-638.
In excitation–emission fluorescence spectroscopy, the simultaneous quantitative prediction and qualitative resolution of mixtures of fluorophores using chemometrics is a major challenge because of the scattering and reabsorption effects (turbidity) presented mainly in biomaterials. The measured fluorescence spectra are distorted by multiple scattering and reabsorption events in the surrounding medium, thereby diminishing the performance of the commonly used three-way resolution methods such as parallel factor (PARAFAC) analysis or multivariate curve resolutionalternating least squares (MCR-ALS). In this work we show that spectral loadings and concentration profiles from model mixtures provided using PARAFAC and MCR-ALS are severely distorted by reabsorption and scattering phenomena, although both models fit rather well the experimental data in terms of percentage of the explained variance. The method to correct the fluorescence excitation–emission matrix (EEM) consisted in measuring the optical properties (absorption parameter μa, scattering parameter μs, and anisotropy factor g) of samples and calculating the corresponding transfer function by means of the Monte Carlo simulation method. By applying this transfer function to the measured EEM, it was possible to compensate for reabsorption and scattering effects and to restore the ideal EEM, i.e., the EEM that is due only to fluorophores, without distortions from the absorbers and scatterers that are present. The PARAFAC and MCR-ALS decomposition of the resulting ideal EEMs provided spectral loadings and concentration profiles that matched the true profiles.
Lyes Lakhal; Victor Acha; Thierry Aussenac. Resolution of Fluorophore Mixtures in Biological Media Using Fluorescence Spectroscopy and Monte Carlo Simulation. Applied Spectroscopy 2014, 68, 697 -711.
AMA StyleLyes Lakhal, Victor Acha, Thierry Aussenac. Resolution of Fluorophore Mixtures in Biological Media Using Fluorescence Spectroscopy and Monte Carlo Simulation. Applied Spectroscopy. 2014; 68 (7):697-711.
Chicago/Turabian StyleLyes Lakhal; Victor Acha; Thierry Aussenac. 2014. "Resolution of Fluorophore Mixtures in Biological Media Using Fluorescence Spectroscopy and Monte Carlo Simulation." Applied Spectroscopy 68, no. 7: 697-711.
Lyes Lakhal; Larbi Rhazi; J. Bonhoure; Thierry Aussenac. Predicting rheological behavior of wheat dough based on machine learning and front-face fluorescence spectroscopy on wheat flour. CFW Plexus 2013, 1 .
AMA StyleLyes Lakhal, Larbi Rhazi, J. Bonhoure, Thierry Aussenac. Predicting rheological behavior of wheat dough based on machine learning and front-face fluorescence spectroscopy on wheat flour. CFW Plexus. 2013; (AACCI 2013):1.
Chicago/Turabian StyleLyes Lakhal; Larbi Rhazi; J. Bonhoure; Thierry Aussenac. 2013. "Predicting rheological behavior of wheat dough based on machine learning and front-face fluorescence spectroscopy on wheat flour." CFW Plexus , no. AACCI 2013: 1.
Eija Chiaramonte; L. Rhazi; T. Aussenac; D. Richard White. Amylose and amylopectin in starch by asymmetric flow field-flow fractionation with multi-angle light scattering and refractive index detection (AF4–MALS–RI). Journal of Cereal Science 2012, 56, 457 -463.
AMA StyleEija Chiaramonte, L. Rhazi, T. Aussenac, D. Richard White. Amylose and amylopectin in starch by asymmetric flow field-flow fractionation with multi-angle light scattering and refractive index detection (AF4–MALS–RI). Journal of Cereal Science. 2012; 56 (2):457-463.
Chicago/Turabian StyleEija Chiaramonte; L. Rhazi; T. Aussenac; D. Richard White. 2012. "Amylose and amylopectin in starch by asymmetric flow field-flow fractionation with multi-angle light scattering and refractive index detection (AF4–MALS–RI)." Journal of Cereal Science 56, no. 2: 457-463.
Lyes Lakhal; Victor Acha; Thierry Aussenac. PARAFAC analysis of front-face fluorescence data: Absorption and scattering effects assessed by means of Monte Carlo simulations. Chemometrics and Intelligent Laboratory Systems 2012, 116, 112 -122.
AMA StyleLyes Lakhal, Victor Acha, Thierry Aussenac. PARAFAC analysis of front-face fluorescence data: Absorption and scattering effects assessed by means of Monte Carlo simulations. Chemometrics and Intelligent Laboratory Systems. 2012; 116 ():112-122.
Chicago/Turabian StyleLyes Lakhal; Victor Acha; Thierry Aussenac. 2012. "PARAFAC analysis of front-face fluorescence data: Absorption and scattering effects assessed by means of Monte Carlo simulations." Chemometrics and Intelligent Laboratory Systems 116, no. : 112-122.
Knowledge of glutenin subunit composition is important for the prediction of the genetic potential of breeding lines as these proteins are known to be responsible for the main differences in bread-making quality. In this study, a commercial high throughput microchip capillary electrophoresis-sodium dodecyl sulfate (microchip CE) platform, LabChip 90, was evaluated for qualitative and quantitative analyses of HMW-GS. 130 French common wheat varieties of known composition were analyzed for rapid identification and the allocation of individual HMW-GS. In addition, the HMW-GS were individually quantified and the ratio of HMW-GS to LMW-GS was determined for genotype comparison. The microchip CE analysis provides comparable resolution and sensitivity to conventional RP-HPLC for identification of the HMW-GS but at a time scale of approximately 100 times faster (45 s per sample analysis versus 80 min for RP-HPLC). The results show that the high throughput microchip CE method can be used for routine identification and quantitation of glutenin subunits, in particular for screening wheat quality and wheat cultivar development activities where large numbers of samples are to be evaluated.
L. Rhazi; A.-L. Bodard; B. Fathollahi; Thierry Aussenac. High throughput microchip-based separation and quantitation of high-molecular-weight glutenin subunits. Journal of Cereal Science 2009, 49, 272 -277.
AMA StyleL. Rhazi, A.-L. Bodard, B. Fathollahi, Thierry Aussenac. High throughput microchip-based separation and quantitation of high-molecular-weight glutenin subunits. Journal of Cereal Science. 2009; 49 (2):272-277.
Chicago/Turabian StyleL. Rhazi; A.-L. Bodard; B. Fathollahi; Thierry Aussenac. 2009. "High throughput microchip-based separation and quantitation of high-molecular-weight glutenin subunits." Journal of Cereal Science 49, no. 2: 272-277.