The effect of different rates of biochar on selected soil properties and enzymatic activity 48, 60, and 72 months after biochar application to soil was investigated. Soil enzymatic activity (dehydrogenase—ADh; phosphatase—Aph; urease—AU), the organic carbon content (TOC), the total nitrogen content (Nt), the mineral nitrogen content (NH4+, NO3−), and soil pH were determined. The study was conducted on Haplic Podzol originating from glaciofluvial fine-grained loamy sand. Biochar was applied to soil under winter rye (Secale cereale L.) at rates of 10 (BC10), 20 (BC20), and 30 t ha−1 (BC30). Plots with biochar-unamended soil were the control treatment (BC0). The pH, TOC, and Nt content in the biochar-amended soil were higher compared to the control soil. A broader C:N ratio was found in the BC0 soil compared to BC10, BC20, and BC30. With increasing biochar rate, the content of the ammonium nitrogen form (NH4+) decreased and was statistically lower than in the control soil (BC0). The soil in the BC20 and BC30 treatments was characterized by the highest content of NO3-, whereas the lowest nitrate nitrogen content was found in the control soil (BC0). Biochar application increased soil enzymatic activity. Dehydrogenase activity increased with increasing biochar rate. As far as phosphatase and urease activity is concerned, a similar relationship was not observed. In this case, the soil amended with biochar at a rate of 20 t ha−1 (BC20) was characterized by the highest phosphatase and urease activity.
This paper presents an ecotoxicological evaluation of soil fertilized with biogas digestate (BD) or mining waste (MS). The study was performed under pot experiment conditions. BD was added at a dose of 1.5 and 3 % to the soil. MS was applied at a dose of 10 and 20 %. Samples were collected at the beginning of the experiment and after 180 and 360 days from the start of the study. In addition, a parallel experiment with the addition of CaO was done. This was designed to eliminate the adverse effect of low soil pH on the test organisms. A battery of ecotoxicological tests was used based on tests with plants (Phytotoxkit F), microorganisms (Microtox®), and crustaceans (Daphtoxkit F). In most cases, the obtained results showed that the investigated wastes had a stimulating effect on the growth of Lepidium sativum roots. The highest content of BD was an exception since it inhibited the growth of L. sativum roots. After adding BD and MS, both the luminescence of Vibrio fischeri and the mortality of Daphnia magna was at a similar level to that in the control soil. An exception was the significant increase in the mortality of D. magna after 48 h for soil with 3 % BD. The tests performed after 6 and 12 months showed that, with time, the toxicity of the waste-amended soil fluctuated, but ultimately decreased in most cases compared to the control. The effect of the addition of CaO on the examined toxicity parameters was different and depended on the experimental variant.
A field study was conducted at the Certified Organic Farm in Poland over the period 2014–2016. This study evaluated weed infestation and seed yield of the lentil varieties ‘Tina’ and ‘Anita’, as well as of a mixture of these two varieties, sole cropped and row intercropped with naked oats as a supporting crop. Additionally, lentil was sown at a different row spacing of 20 and 25 cm. The lentil variety ‘Anita’ produced 25.3% higher yields than var. ‘Tina’. Weight of 1000 seed, number of pods per plant, and first pod height did not differ significantly in the treatments with the lentil varieties. The lentil seed yield obtained in the treatments with a supporting crop was lower by 9.4% compared with the sole cropped plots. In turn, the 1000 seed weight was 3.9% higher in the treatment where a supporting crop was used. The presence of oats as a supporting crop in lentil crop allowed crop competitiveness against weeds to be increased significantly, thus reducing their total number and dry weight by 5.3% and 30.5%, respectively. Sowing lentil at different row spacings did not have a significant effect on seed yield and weed infestation in crop. The greatest diversity of weed species was found in the treatments where the mixture of the lentil varieties was sown and in the treatment with the smaller row spacing. Intercropping of lentil with oats resulted in reduced occurrence of monocotyledonous weeds such as Echinochloa crus-galli and Elymus repens. The presence of oat as a supporting crop can effectively reduce the pressure from weeds without significantly reducing lentil cultivar yield in organic farming.