Water hyacinth (WH) is an invasive aquatic macrophyte that dominates freshwater bodies across the world. However, due to its rapid growth rate and wide-spread global presence, WH could offer great potential as a biomass feedstock, including for bioenergy generation. This study compares different integration strategies of hydrothermal carbonisation (HTC) and anaerobic digestion (AD) using WH, across a range of temperatures. These include (i) hydrochar combustion and process water digestion, (ii) hydrochar digestion, (iii) slurry digestion. HTC reactions were conducted at 150 °C, 200 °C, and 250 °C. Separation of hydrochars for combustion and process waters for digestion offers the most energetically-feasible valorisation route. However, hydrochars produced from WH display slagging and fouling tendencies; limiting their use in large-scale combustion. AD of WH slurry produced at 150 °C appears to be energetically-feasible and has the potential to also be a viable integration strategy between HTC and AD, using WH.

Thermoanaerobacter species have recently been observed to reduce carboxylic acids to their corresponding alcohols. The present investigation shows that Thermoanaerobacter pseudoethanolicus converts C2–C6 short-chain fatty acids (SCFAs) to their corresponding alcohols in the presence of glucose. The conversion yields varied from 21% of 3-methyl-1-butyrate to 57.9% of 1-pentanoate being converted to their corresponding alcohols. Slightly acidic culture conditions (pH 6.5) was optimal for the reduction. By increasing the initial glucose concentration, an increase in the conversion of SCFAs reduced to their corresponding alcohols was observed. Inhibitory experiments on C2–C8 alcohols showed that C4 and higher alcohols are inhibitory to T. pseudoethanolicus suggesting that other culture modes may be necessary to improve the amount of fatty acids reduced to the analogous alcohol. The reduction of SCFAs to their corresponding alcohols was further demonstrated using ¹³C-labelled fatty acids and the conversion was followed kinetically. Finally, increased activity of alcohol dehydrogenase (ADH) and aldehyde oxidation activity was observed in cultures of T. pseudoethanolicus grown on glucose as compared to glucose supplemented with either 3-methyl-1-butyrate or pentanoate, using both NADH and NADPH as cofactors, although the presence of the latter showed higher ADH and aldehyde oxidoreductase (ALDH) activity.