Introduction: Soil microorganisms are the key factors in elucidating the effects of thinning on tree growth performance, but the effects of vegetation and soil on the species composition and function of soil microorganisms after thinning are still not well elaborated.Methods: The effects of thinning on understory vegetation diversity, soil physicochemical properties and soil microbial community composition were investigated in a thinning trial plantation ofCryptomeria japonicavar.sinensis, including four thinning intensities (control: 0%, LIT: 20%, MIT: 30% and HIT: 40%), and the relationships of the microbial community structure with the understory vegetation diversity and soil properties were assessed.Results: The results showed that thinning had a greater effect on the diversity of the shrub layer than the herb layer. The soil bulk density and the contents of soil organic matter, total potassium and nitrogen increased with increasing thinning intensities. The Shannon and Chao indices of soil bacteria and fungi were significantly lower in the LIT, MIT and HIT treatments than in the control. Thinning can significantly increase the abundance ofProteobacteriaandActinobacteria, and higher thinning intensities led to a higher relative abundance ofAscomycotaand a lower relative abundance ofBasidiomycota,Rozellomycota, andMortierellomycota. Redundancy analysis indicated that soil physicochemical properties rather than understory vegetation diversity were the main drivers of microbial communities, and fungi were more sensitive to soil properties than bacteria. Functional prediction showed that thinning significantly reduced the potential risk of human diseases and plant pathogens, and the nitrogen fixation capacity of bacteria was the highest in the HIT treatment. Thinning significantly increased the relative abundance of cellulolysis and soil saprotrophs in bacteria and fungi.Conclusion: The findings provide important insights into the effects of thinning onC. japonicavar.sinensisplantation ecosystems, which is essential for developing thinning strategies to promote their ecological and economic benefits.

Knot-related defects are the major cause of timber quality degradation, and diminishing this kind of defects is an important issue in forest management. For the purpose of clear-wood production, knot attributes and branch occlusion of Betula alnoides under natural pruning were investigated in a 14-year-old experimental plantation with five planting densities ranging from 500 to 3333 stems per hectare in southern China, and a total of 1325 occluded branches from 30 trees were sampled and dissected. The mean occluded branch diameter (OBD), radius of knots and branch insertion angle (IA) decreased significantly with increasing planting density. Planting with high stocking density significantly reduced the frequency of thick occluded branches (diameter ≥ 20 mm) while increasing the frequency of small ones (diameter < 10 mm). Branch occlusion time (OT) also tended to increase with decreasing planting density. The results of generalized linear mixed models showed that OBD was the major factor influencing OT, radius of dead portion of knot (RDP), total radius of knot (TRK) and IA. In addition, OT was positively correlated with RDP but negatively correlated with stem diameter growth rate during branch occlusion (SDGR). Silvicultural strategies with appropriate planting density for large-diameter clear-wood production of B. alnoides were discussed.

Branch development in the lower part of stem is critical to both early stem growth and wood quality of the most valuable section of tree, and its regulation through planting density has always been greatly concerned. Here the effect of planting density on branch development was examined in a five-year-old plantation of Betula alnoides with six planting densities (625, 833, 1111, 1250, 1667, and 2500 stems per hectare (sph)) in Guangdong Province, South China. Branch quantity (number, proportion, and density), morphology (diameter, length, and angle), position (height and orientation), and branch status (dead or alive) were investigated for 54 dominant or co-dominant trees under six treatments of planting density after the growth of each tree was measured. Factors influencing branch development were also explored by mixed modelling. The results showed that the mean tree heights of 1250 and 1667 sph treatments were higher than those of other planting density treatments. The quantity of live branches decreased with increasing planting density. However, planting density had no significant effect on the number of all branches, and there existed no remarkable difference in branch number and proportion among four orientations. As for branch morphology, only the largest branch diameter had a significantly negative correlation with planting density. In addition, high planting density significantly increased the height of the largest branch within the crown. Mixed effects models indicated that branch diameter, length, and angle were closely correlated with each other, and they were all in positively significant correlation to the branch height at the stem section below six meters. It was concluded that properly increasing planting density will promote natural pruning, improve early branch control, and be beneficial for wood production from the most valuable section of the stem.

Both crown and branch characteristics greatly affect tree growth and timber quality, and their development is closely related to planting density (PD). Here crown and branch attributes of Betula alnoides were investigated in a 14-year-old experimental plantation in southern China with five planting densities ranging from 500 to 3333 stems per hectare (sph). The results showed that high PD significantly reduced crown and branch sizes. The ratios of crown diameter to stem diameter at breast height at the stand level and for dominant and co-dominant trees were almost constant regardless of PD. PD also had no significant influence on the number and angle of branches, and only planting with the highest density significantly increased branch density and mortality. Insertion angle (IA) of dead branches was greater than that of live branches and these differences were significant at the two low planting densities (833 and 500 sph). Linear mixed models revealed that branch diameter (BD) was negatively correlated with its relative height in the crown and IA. The binary-logistic regression model indicated that branch status (live or dead) was codetermined by BD and PD. These findings can help farmers cultivate high-quality wood of B. alnoides and obtain a higher economic return.