Hypertension is an important global public health issue because of its high morbidity as well as the increased risk of other diseases. Recent studies have indicated that the development of hypertension is related to the dysbiosis of the gut microbiota in both animals and humans. In this review, we outline the interaction between gut microbiota and hypertension, including gut microbial changes in hypertension, the effect of microbial dysbiosis on blood pressure (BP), indicators of gut microbial dysbiosis in hypertension, and the microbial genera that affect BP at the taxonomic level. For example, increases in Lactobacillus, Roseburia, Coprococcus, Akkermansia, and Bifidobacterium are associated with reduced BP, while increases in Streptococcus, Blautia, and Prevotella are associated with elevated BP. Furthermore, we describe the potential mechanisms involved in the regulation between gut microbiota and hypertension. Finally, we summarize the commonly used treatments of hypertension that are based on gut microbes, including fecal microbiota transfer, probiotics and prebiotics, antibiotics, and dietary supplements. This review aims to find novel potential genera for improving hypertension and give a direction for future studies on gut microbiota in hypertension.

In the Arctic ecosystems, fungi are crucial for interactions between soil and plants, the cycling of nutrients, and the transport of carbon. To date, no studies have been conducted to thoroughly examine the mycobiome and its functional role in various habitats of the High Arctic region. The aim was to unravel the mycobiome in the nine habitats (i.e., soil, lichen, vascular plant, moss, freshwater, seawater, marine sediment, dung, and marine alga) in the Ny-Ålesund Region (Svalbard, High Arctic) using a high-throughput sequencing approach. A total of 10,419 ASVs were detected. Among them, 7535 ASVs were assigned to unidentified phyla, while the remaining 2884 ASVs were assigned to 11 phyla, 33 classes, 81 orders, 151 families, 278 genera, and 261 species that were known. The distribution of the mycobiome was driven by habitat specificity, indicating that habitat filtering is a crucial factor influencing the fungal assemblages at a local scale in this High Arctic region. Six growth forms and 19 fungal guilds were found. The ecological guild (e.g., lichenized, ectomycorrhizal) and growth form (e.g., yeast, thallus photosynthetic) varied significantly among various habitats. In addition, the occurrence of 31 fungal species that are considered to be potential pathogens was determined. These results will increase our understanding of fungal diversity and its functional significance in this distinctive High Arctic area and thereby establish the groundwork for prediction about how the mycobiome will alter in various environments as a result of anticipated climate change.

Isolating relevant microorganisms is still a substantial challenge that limits the use of bacteria in the maintenance of human health. To confirm which media and which bacterial colony densities can enrich certain kinds of bacteria, we selected eight common media and used them to enrich the gut microorganisms on agar plates. Then, we calculated the numbers of bacterial colonies and collected the bacterial culture mixtures from each kind of medium. Using the Illumina HiSeq platform, we analyzed the composition and diversity of the culture-enriched gut bacterial community. Our data suggested that medium supplemented with blood could increase the diversity of the bacterial community. In addition, beef powder and peptone could significantly change the culture-enriched bacterial community. A moderate density (100–150 colony-forming units per plate) was optimal for obtaining the highest diversity on the agar. Similarly, membrane transport was significantly enriched in the moderate-density group, which indicated a more active metabolism in this density range. Overall, these results reveal the optimal culture conditions, including the densities of colonies and nutritional components for various gut bacteria, that provide a novel strategy for isolating bacteria in a way that is targeted and avoids blinded and repetitive work.

Particulate matter (PM) has been recognized as a significant environmental contaminant with substantial effects on human health, although the impact of PM pollution on the skin microbiota is less understood. In this study, 78 skin microbiota samples from volunteers were obtained during periods of haze and non-haze in the spring and winter. The diversity, composition, and co-occurrence networks of the skin bacterial community were revealed using high-throughput sequencing. Acinetobacter sp. XSB125 and Pseudomonas sp. XSB6 were isolated and cocultured with PM collected during haze days. Significant seasonal variations were observed in the skin bacterial community, with winter samples showing greater diversities than spring samples. Supervised partial least squares discriminant analysis indicated that PM pollution influenced the skin bacterial community composition. Stronger interactions were detected in the network structure of the skin bacterial community during haze days. Differential and random forest analyses revealed that Acinetobacter and Pseudomonas, which are important resistant opportunistic pathogens, were significantly enriched during haze days in winter. To confirm the increases in Acinetobacter and Pseudomonas during haze days, an Acinetobacter strain and a Pseudomonas strain were isolated and cultured with the PM we collected during haze days. In vitro experiments confirmed that PM promoted the growth of the Acinetobacter and Pseudomonas strains. Function analysis revealed increased metabolic function and enrichment of antibiotic resistance- and pathogenicity-related functions during haze days, including the beta-lactamase gene and attachment invasion locus protein. These findings reveal the complex interplay between PM pollution and the skin microbiota, highlighting the need for further research into mitigation strategies to protect the public health from PM exposure.

Hypertension remains a major risk factor worldwide. The traditional Chinese medicine Achyranthes bidentata (Ab) regulates the gut microbiota, but how its anti-hypertensive activity relates to the gut microbiota and metabolism remains unclear. Here we show that Ab significantly lowers blood pressure and ameliorates renal injury in L-NAME/high-salt hypertensive mice, without significant changes in circulating inflammatory cytokines or aldosterone. High-throughput 16 S rRNA gene amplicon sequencing revealed significant treatment-driven community divergence that widened over time, whereas α-diversity based on the Shannon, Faith PD, and Observed-feature indices showed no significant difference after Ab treatment. Differential abundance and random-forest analyses identified Dubosiella and Lactobacillus as the principal Ab-enriched genera negatively correlated with blood pressure, with the relative abundance of Dubosiella expanding from 0.1% to 26.5%. Metabolomic profiling showed that Ab remodeled gut arachidonic acid metabolism, with elevated PGE2 p-acetamidophenyl ester. Network analysis associated Dubosiella abundance with arachidonic acid–related metabolites and lower blood pressure, and Procrustes analysis confirmed microbiota–metabolome concordance. In vitro, Ab promoted the growth and biofilm formation of Dubosiella newyorkensis DSM103457, supporting the Ab-associated increase in the relative abundance of Dubosiella . These data indicate that Ab acts as an anti-hypertensive herbal prebiotic whose effect is associated with an increase in the relative abundance of Dubosiella and with remodeling of arachidonic acid metabolism, providing correlative evidence for microbiota-targeted hypertension therapy.