The hydrogen-bonded organic frameworks (HOFs) are a class of porous materials with crystalline frame structures, which are self-assembled from organic structures by hydrogen bonding in non-covalent bonds π-π packing and van der Waals force interaction. HOFs are widely used in environmental remediation due to their high specific surface area, ordered pore structure, pore modifiability, and post-synthesis adjustability of various physical and chemical forms. This work summarizes some rules for constructing stable HOFs and the synthesis of HOF-based materials (synthesis of HOFs, metallized HOFs, and HOF-derived materials). In addition, the applications of HOF-based materials in the field of environmental remediation are introduced, including adsorption and separation (NH₃, CO₂/CH₄ and CO₂/N₂, C₂H₂/C₂H_e and C_eH₆, C₂H₂/CO₂, Xe/Kr, etc.), heavy metal and radioactive metal adsorption, organic dye and pesticide adsorption, energy conversion (producing H₂ and CO₂ reduced to CO), organic dye degradation and pollutant sensing (metal ion, aniline, antibiotic, explosive steam, etc.). Finally, the current challenges and further studies of HOFs (such as functional modification, molecular simulation, application extension as remediation of contaminated soil, and cost assessment) are discussed. It is hoped that this work will help develop widespread applications for HOFs in removing a variety of pollutants from the environment.

The urea-crosslinked starch (UcS) film has a major drawback of very rapid biodegradability when applied as slow release fertilizer in soil. Lignin reinforcement of the UcS was used to prepare composite films, aimed to reduce the starch biodegradability and slow the release of nitrogen in aerobic soil condition. Study results revealed that mineralization of the composite films was delayed from 6.40 to 13.58% more than UcS film. Inhibition of composite films mixing with soil, the Michaelis-Menten reaction rates for α -amylase were inhibited ~1.72–2.03 times whereas the Michaelis-Menten reaction rates for manganese peroxidase were increased ~1.07–1.41 times compared to UcS film. Saccharides–glucose, maltose and maltotriose demonstrated that their rates of formation (zero-order reaction) and depletion (first-order reaction); both were slowed more in aerobic soil which received the composite films. Increasing of lignin in composite films, the acid to aldehyde ratios of vanillyl and syringyl phenols of the lignin declined from 1.18 to 1.17 (~0.76%) and 1.59–1.56 (~1.78%), respectively. The diffusivity of nitrogen was effectively slowed 0.66–0.94 times by the lignin in composite films and showed a “Fickian diffusion” mechanism (release exponent n =0.095–0.143).

In this review, we present the research from 2013 to 2022 about the character of ionic liquids, the categories of phytochemicals, and the reasons for selecting imidazolium ionic liquids for phytochemical extraction. Then we introduce the structural formulae of the imidazolium ionic liquids commonly used in the extraction of phytochemicals, the methods used to prepare imidazolium ionic liquids, and a comprehensive introduction of how imidazolium ionic liquids are applied to extract phytochemicals from plants. Importantly, we discuss the strategies for studying the extraction mechanisms of imidazolium ionic liquids to extract phytochemicals, and the recovery methods regarding imidazolium ionic liquids and their recyclability are analyzed. Then the toxicity in imidazolium ionic liquids is pointed out. Finally, the challenges and prospects of extracting phytochemicals by imidazolium ionic liquids are summarized, and they are expected to provide some references for researchers.

Pakistan is an agricultural country and fisheries play a very important role in the economic development of the country. Different diseases are prevalent in Pakistani fish but information related to the causative agents is not well-known. Keeping in view the significance of bacterial pathogens as the causative agents of multiple fish diseases, the present study was conducted for identification, characterization and analysis of virulence genes of Aeromonas spp. isolated from diseased fishes. A total of fifty fish samples having multiple clinical indications were collected from different fish farms of district Kasur, Punjab Pakistan. For isolation of Aeromonas spp. samples were enriched and inoculated on Aeromonas isolation medium. Isolates were identified and characterized by different biochemical tests, Analytical Profile Index (API) 20E kit and Polymerase Chain Reaction (PCR) assays. All isolates were screened for three putative virulence genes including aerolysin (aer), haemolysin (hyl) and heat labile cytotonic enterotoxin (alt). Seven isolates of Aeromonas (A.) hydrophila were retrieved and identified based on API 20E. These isolates were further confirmed as A. hydrophila on the basis of PCR assays. Three isolates were detected positive for the presence of virulence genes (alt and hyl). Whereas aerolysin (aer) gene was not present in any of A. hydrophila isolates. The present study confirmed A. hydrophila as the causative agent of epizootic ulcerative syndrome and motile Aeromonas septicemia in fish farms of district Kasur, Punjab Pakistan. Moreover, detection of two virulence genes (alt and hyl) in A. hydrophila isolates is a threat for fish consumers of study area.

Green biomass is a renewable and biodegradable material that has the potential use to trap urea to develop a high-efficiency urea fertilizer for crops’ better performance. Current work examined the morphology, chemical composition, biodegradability, urea release, soil health, and plant growth effects of the SRF films subjected to changes in the thickness of 0.27, 0.54, and 1.03 mm. The morphology was examined by Scanning Electron Microscopy, chemical composition was analyzed by Infrared Spectroscopy, and biodegradability was assessed through evolved CO₂ and CH₄ quantified through Gas Chromatography. The chloroform fumigation technique was used for microbial growth assessment in the soil. The soil pH and redox potential were also measured using a specific probe. CHNS analyzer was used to calculate the total carbon and total nitrogen of the soil. A plant growth experiment was conducted on the Wheat plant (Triticum sativum). The thinner the films, the more they supported the growth and penetration of the soil’s microorganisms mainly the species of fungus possibly due to the presence of lignin in films. The fingerprint regions of the infrared spectrum of SRF films showed all films in soil changed in their chemical composition due to biodegradation but the increase in the thickness possibly provides resistance to the films’ losses. The higher thickness of the film delayed the rate and time for biodegradation and the release of methane gas in the soil. The 1.03 mm film (47% in 56 days) and 0.54 mm film (35% in 91 days) showed the slowest biodegradability as compared to the 0.27 mm film with the highest losses (60% in 35 days). The slow urea release is more affected by the increase in thickness. The Korsymer Pappas model with release exponent value of < 0.5 explained the release from the SRF films followed the quasi-fickian diffusion and also reduced the diffusion coefficient for urea. An increase in the pH and decrease in the redox potential of the soil is correlated with higher total organic content and total nitrogen in the soil in response to amending SRF films with variable thickness. Growth of the wheat plant showed the highest average plant length, leaf area index and grain per plant in response to the increase in the film’s thickness. This work developed an important knowledge to enhance the efficiency of film encapsulated urea that can better slow the urea release if the thickness is optimized.