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Prof. Stefano Manfredini
Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy

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0 Isolation
0 Synthesis
0 natural compounds
0 UV protection

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Journal article
Published: 21 September 2020 in Molecules
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In the search for scaffolds for multifunctional compounds we investigated the structure activity relationship of a class of benzimidazole derivatives bearing 5-membered ring. The newly synthesized and the already known compounds were divided into three classes that present different substituent at 5 position of the benzimidazole ring (-H, -COOH or –SO3H) and different heterocycle at position 2 (thiophene, furan or pyrrole). All the derivatives were synthesized and tested to determine their photoprotective profile against UV rays, in vitro antioxidant capacity against different radicals (DPPH and FRAP test), antifungal inhibitory activity (dermatophytes and Candida albicans), antiviral and antiproliferative activity. A Structure-Activity Relationship study indicated compound 10, bearing a pyrrole heterocycle on the benzimidazole ring, as the best multifunctional derivative of the series and as potential candidate for the development of drugs especially in case of melanoma.

ACS Style

Ernestine Nicaise Djuidje; Elisa Durini; Sabrina Sciabica; Elena Serra; Jan Balzarini; Sandra Liekens; Stefano Manfredini; Silvia Vertuani; Anna Baldisserotto. Skin Damages—Structure Activity Relationship of Benzimidazole Derivatives Bearing a 5-Membered Ring System. Molecules 2020, 25, 4324 .

AMA Style

Ernestine Nicaise Djuidje, Elisa Durini, Sabrina Sciabica, Elena Serra, Jan Balzarini, Sandra Liekens, Stefano Manfredini, Silvia Vertuani, Anna Baldisserotto. Skin Damages—Structure Activity Relationship of Benzimidazole Derivatives Bearing a 5-Membered Ring System. Molecules. 2020; 25 (18):4324.

Chicago/Turabian Style

Ernestine Nicaise Djuidje; Elisa Durini; Sabrina Sciabica; Elena Serra; Jan Balzarini; Sandra Liekens; Stefano Manfredini; Silvia Vertuani; Anna Baldisserotto. 2020. "Skin Damages—Structure Activity Relationship of Benzimidazole Derivatives Bearing a 5-Membered Ring System." Molecules 25, no. 18: 4324.

Review
Published: 11 June 2020 in Cosmetics
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A polar head and an apolar tail chemically characterize surfactants, they show different properties and are categorized by different factors such as head charge and molecular weight. They work by reducing the surface tension between oil and water phases to facilitate the formation of one homogeneous mixture. In this respect, they represent unavoidable ingredients, their main application is in the production of detergents, one of if not the most important categories of cosmetics. Their role is very important, it should be remembered that it was precisely soaps and hygiene that defeated the main infectious diseases at the beginning of the last century. Due to their positive environmental impact, the potential uses of microbial sourced surfactants are actively investigated. These compounds are produced with different mechanisms by microorganisms in the aims to defend themselves from external threats, to improve the mobility in the environment, etc. In the cosmetic field, biosurfactants, restricted in the present work to those described above, can carry high advantages, in comparison to traditional surfactants, especially in the field of sustainable and safer approaches. Besiede this, costs still remain an obsatcle to their diffusion; in this regard, exploration of possible multifunctional actions could help to contain application costs. To highlight their features and possible multifunctional role, on the light of specific biological profiles yet underestimated, we have approached the present review work.

ACS Style

Hamid-Reza Ahmadi-Ashtiani; Anna Baldisserotto; Elena Cesa; Stefano Manfredini; Hossein Sedghi Zadeh; Mostafa Ghafori Gorab; Maryam Khanahmadi; Samin Zakizadeh; Piergiacomo Buso; Silvia Vertuani. Microbial Biosurfactants as Key Multifunctional Ingredients for Sustainable Cosmetics. Cosmetics 2020, 7, 46 .

AMA Style

Hamid-Reza Ahmadi-Ashtiani, Anna Baldisserotto, Elena Cesa, Stefano Manfredini, Hossein Sedghi Zadeh, Mostafa Ghafori Gorab, Maryam Khanahmadi, Samin Zakizadeh, Piergiacomo Buso, Silvia Vertuani. Microbial Biosurfactants as Key Multifunctional Ingredients for Sustainable Cosmetics. Cosmetics. 2020; 7 (2):46.

Chicago/Turabian Style

Hamid-Reza Ahmadi-Ashtiani; Anna Baldisserotto; Elena Cesa; Stefano Manfredini; Hossein Sedghi Zadeh; Mostafa Ghafori Gorab; Maryam Khanahmadi; Samin Zakizadeh; Piergiacomo Buso; Silvia Vertuani. 2020. "Microbial Biosurfactants as Key Multifunctional Ingredients for Sustainable Cosmetics." Cosmetics 7, no. 2: 46.

Journal article
Published: 26 May 2020 in Life
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The term “Salus per aquam (SPA) therapies” refers to therapeutic pathways that includes mud therapy. The therapeutic efficacy of a peloid depends on its chemical and mineralogical composition, as well as its technological properties. Considering the increasing use of clay-based products, it becomes essential to characterize peloids from a qualitative and quantitative point of view. Therefore, this research project aimed to develop a protocol that allows characterization of the chemical-physical composition of sludges collected from different areas of the Homogeneous Euganean Hills Hydromineral Basin (B.I.O.C.E.). The study established a comparative study both between different matrices and between the same matrices at different maturation times, including also a comparison with an industrialized product, that can be used at home, which maintains the characteristics of a natural mud. This research was developed studying the pH, grain size distribution, and chemical and mineralogical composition. Peloids are characterized by a neutral/basic pH and are divided into two categories from a granulometric point of view: The chemical composition allowed observation of numerous correlations between oxides present in the samples and to quantify the presence of heavy metals. Mineralogical analysis made it possible to identify and compare the composition of each sample, also according to the maturation time. Thanks to the methods adopted, important correlations were achieved.

ACS Style

Beatrice Bergamaschi; Laura Marzola; Matteo Radice; Stefano Manfredini; Erika Baldini; Chiara Beatrice Vicentini; Elena Marrocchino; Sonia Molesini; Paola Ziosi; Carmela Vaccaro; Silvia Vertuani. Comparative Study of SPA Mud from “Bacino Idrominerario Omogeneo dei Colli Euganei (B.I.O.C.E.)–Italy” and Industrially Optimized Mud for Skin Applications. Life 2020, 10, 78 .

AMA Style

Beatrice Bergamaschi, Laura Marzola, Matteo Radice, Stefano Manfredini, Erika Baldini, Chiara Beatrice Vicentini, Elena Marrocchino, Sonia Molesini, Paola Ziosi, Carmela Vaccaro, Silvia Vertuani. Comparative Study of SPA Mud from “Bacino Idrominerario Omogeneo dei Colli Euganei (B.I.O.C.E.)–Italy” and Industrially Optimized Mud for Skin Applications. Life. 2020; 10 (6):78.

Chicago/Turabian Style

Beatrice Bergamaschi; Laura Marzola; Matteo Radice; Stefano Manfredini; Erika Baldini; Chiara Beatrice Vicentini; Elena Marrocchino; Sonia Molesini; Paola Ziosi; Carmela Vaccaro; Silvia Vertuani. 2020. "Comparative Study of SPA Mud from “Bacino Idrominerario Omogeneo dei Colli Euganei (B.I.O.C.E.)–Italy” and Industrially Optimized Mud for Skin Applications." Life 10, no. 6: 78.

Journal article
Published: 06 May 2020 in Molecules
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Induction of apoptosis is a common chemotherapeutic mechanism to kill cancer cells The thiazole system has been reported over the past decades as a building block for the preparation of anticancer agents. A novel series of 2-arylalkylamino-4-amino-5-(3′,4′,5′-trimethoxybenzoyl)-thiazole derivatives designed as dual inhibitors of tubulin and cyclin-dependent kinases (CDKs) were synthesized and evaluated for their antiproliferative activity in vitro against two cancer cell lines and, for selected highly active compounds, for interactions with tubulin and cyclin-dependent kinases and for cell cycle and apoptosis effects. Structure-activity relationships were elucidated for various substituents at the 2-position of the thiazole skeleton. Among the synthesized compounds, the most active analogues were found to be the p-chlorobenzylamino derivative 8e as well as the p-chloro and p-methoxyphenethylamino analogues 8f and 8k, respectively, which inhibited the growth of U-937 and SK-MEL-1 cancer cell lines with IC50 values ranging from 5.7 to 12.2 μM. On U-937 cells, the tested compounds 8f and 8k induced apoptosis in a time and concentration dependent manner. These two latter molecules did not affect tubulin polymerization (IC50 > 20 μM) nor CDK activity at a single concentration of 10 μM, suggesting alternative targets than tubulin and CDK for the compounds.

ACS Style

Paola Oliva; Valentina Onnis; Elisa Balboni; Ernest Hamel; Francisco Estévez-Sarmiento; José Quintana; Francisco Estévez; Andrea Brancale; Salvatore Ferla; Stefano Manfredini; Romeo Romagnoli. Synthesis and Biological Evaluation of 2-Substituted Benzyl-/Phenylethylamino-4-amino-5-aroylthiazoles as Apoptosis-Inducing Anticancer Agents. Molecules 2020, 25, 2177 .

AMA Style

Paola Oliva, Valentina Onnis, Elisa Balboni, Ernest Hamel, Francisco Estévez-Sarmiento, José Quintana, Francisco Estévez, Andrea Brancale, Salvatore Ferla, Stefano Manfredini, Romeo Romagnoli. Synthesis and Biological Evaluation of 2-Substituted Benzyl-/Phenylethylamino-4-amino-5-aroylthiazoles as Apoptosis-Inducing Anticancer Agents. Molecules. 2020; 25 (9):2177.

Chicago/Turabian Style

Paola Oliva; Valentina Onnis; Elisa Balboni; Ernest Hamel; Francisco Estévez-Sarmiento; José Quintana; Francisco Estévez; Andrea Brancale; Salvatore Ferla; Stefano Manfredini; Romeo Romagnoli. 2020. "Synthesis and Biological Evaluation of 2-Substituted Benzyl-/Phenylethylamino-4-amino-5-aroylthiazoles as Apoptosis-Inducing Anticancer Agents." Molecules 25, no. 9: 2177.

Communication
Published: 15 April 2020 in Pharmaceuticals
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In the search of multifunctional compounds we designed benzimidazole derivatives endowed with phenolic hydroxy groups and a hydrazone moiety as potential radical-scavenger and the antioxidant agents. The target molecules have been prepared by a simple synthetic procedure and tested for their antioxidant activity by DPPH, FRAP, and ORAC test, for photoprotective activity against UV rays and for antiproliferative activity against Colo-38 melanoma cells. Furthermore, two different dermocosmetic formulations were prepared with the compounds endowed with the best antioxidant and photoprotective profile and their release from formulation evaluated using Franz Cells system. High antioxidant activity is related to the presence of at least two hydroxy groups on arylidene moiety of benzimidazoles. Structure activity analysis revealed that the position of hydroxy groups is crucial for antioxidant activity as well as the presence of a 2-hydroxy-4-(diethylamino)arylidene group. The same correlation pattern was found to be related to photoprotective activity resulting in an UVA Protection Factor better than the commercial solar filter PBSA and antiproliferative activity against melanoma cells without producing cytotoxicity on normal keratinocytes. The release analysis indicated that high antioxidant activities are achieved with limited release at concentration compatible with the use as UV sunscreen filter.

ACS Style

Anna Baldisserotto; Monica Demurtas; Ilaria Lampronti; Massimo Tacchini; Davide Moi; Gianfranco Balboni; Silvia Vertuani; Stefano Manfredini; Valentina Onnis. In-Vitro Evaluation of Antioxidant, Antiproliferative and Photo-Protective Activities of Benzimidazolehydrazone Derivatives. Pharmaceuticals 2020, 13, 68 .

AMA Style

Anna Baldisserotto, Monica Demurtas, Ilaria Lampronti, Massimo Tacchini, Davide Moi, Gianfranco Balboni, Silvia Vertuani, Stefano Manfredini, Valentina Onnis. In-Vitro Evaluation of Antioxidant, Antiproliferative and Photo-Protective Activities of Benzimidazolehydrazone Derivatives. Pharmaceuticals. 2020; 13 (4):68.

Chicago/Turabian Style

Anna Baldisserotto; Monica Demurtas; Ilaria Lampronti; Massimo Tacchini; Davide Moi; Gianfranco Balboni; Silvia Vertuani; Stefano Manfredini; Valentina Onnis. 2020. "In-Vitro Evaluation of Antioxidant, Antiproliferative and Photo-Protective Activities of Benzimidazolehydrazone Derivatives." Pharmaceuticals 13, no. 4: 68.

Journal article
Published: 07 April 2020 in Molecules
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Two novel series of compounds based on the 4,5,6,7-tetrahydrothieno[2,3-c]pyridine and 4,5,6,7-tetrahydrobenzo[b]thiophene molecular skeleton, characterized by the presence of a 3′,4′,5′-trimethoxyanilino moiety and a cyano or an alkoxycarbonyl group at its 2- or 3-position, respectively, were designed, synthesized, and evaluated for antiproliferative activity on a panel of cancer cell lines and for selected highly active compounds, inhibition of tubulin polymerization, and cell cycle effects. We have identified the 2-(3′,4′,5′-trimethoxyanilino)-3-cyano-6-methoxycarbonyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine derivative 3a and its 6-ethoxycarbonyl homologue 3b as new antiproliferative agents that inhibit cancer cell growth with IC50 values ranging from 1.1 to 4.7 μM against a panel of three cancer cell lines. Their interaction with tubulin at micromolar levels leads to the accumulation of cells in the G2/M phase of the cell cycle and to an apoptotic cell death. The cell apoptosis study found that compounds 3a and 3b were very effective in the induction of apoptosis in a dose-dependent manner. These two derivatives did not induce cell death in normal human peripheral blood mononuclear cells, suggesting that they may be selective against cancer cells. Molecular docking studies confirmed that the inhibitory activity of these molecules on tubulin polymerization derived from binding to the colchicine site.

ACS Style

Romeo Romagnoli; Filippo Prencipe; Paola Oliva; Barbara Cacciari; Jan Balzarini; Sandra Liekens; Ernest Hamel; Andrea Brancale; Salvatore Ferla; Stefano Manfredini; Matteo Zurlo; Alessia Finotti; Roberto Gambari. Synthesis and Biological Evaluation of New Antitubulin Agents Containing 2-(3′,4′,5′-trimethoxyanilino)-3,6-disubstituted-4,5,6,7-tetrahydrothieno[2,3-c]pyridine Scaffold. Molecules 2020, 25, 1690 .

AMA Style

Romeo Romagnoli, Filippo Prencipe, Paola Oliva, Barbara Cacciari, Jan Balzarini, Sandra Liekens, Ernest Hamel, Andrea Brancale, Salvatore Ferla, Stefano Manfredini, Matteo Zurlo, Alessia Finotti, Roberto Gambari. Synthesis and Biological Evaluation of New Antitubulin Agents Containing 2-(3′,4′,5′-trimethoxyanilino)-3,6-disubstituted-4,5,6,7-tetrahydrothieno[2,3-c]pyridine Scaffold. Molecules. 2020; 25 (7):1690.

Chicago/Turabian Style

Romeo Romagnoli; Filippo Prencipe; Paola Oliva; Barbara Cacciari; Jan Balzarini; Sandra Liekens; Ernest Hamel; Andrea Brancale; Salvatore Ferla; Stefano Manfredini; Matteo Zurlo; Alessia Finotti; Roberto Gambari. 2020. "Synthesis and Biological Evaluation of New Antitubulin Agents Containing 2-(3′,4′,5′-trimethoxyanilino)-3,6-disubstituted-4,5,6,7-tetrahydrothieno[2,3-c]pyridine Scaffold." Molecules 25, no. 7: 1690.

Review
Published: 24 March 2020 in International Journal of Molecular Sciences
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Cutaneous stem cells, gained great attention in the field of regenerative medicine as a potential therapeutic target for the treatment of skin and hair disorders and various types of skin cancers. Cutaneous stem cells play a key role in several processes like the renovation of skin structures in the condition of homeostasis and after injuries, the hair follicle growth and the reconstruction and production of melanocytes. Thus, gaining effective access to skin stem cells for therapeutic interventions that often involve active molecules with non-favorable characteristics for skin absorption is a valuable achievement. The topical route with high patient compliance and several other benefits is gaining increasing importance in basic and applied research. However, the major obstacle for topical drug delivery is the effective barrier provided by skin against penetration of the vast majority of exogenous molecules. The research in this field is focusing more and more on new strategies to circumvent and pass this barrier effectively. In this article the existing approaches are discussed considering physical and chemical methods along with utilization of novel drug delivery systems to enhance penetration of drugs to the skin. In particular, attention has been paid to studies finalized to the delivery of molecules to cutaneous stem cells with the aim of transferring signals, modulating their metabolic program, inducing physiological modifications and stem cell gene therapy.

ACS Style

Hamid-Reza Ahmadi-Ashtiani; Parisa Bishe; Anna Baldisserotto; Piergiacomo Buso; Stefano Manfredini; Silvia Vertuani. Stem Cells as a Target for the Delivery of Active Molecules to Skin by Topical Administration. International Journal of Molecular Sciences 2020, 21, 2251 .

AMA Style

Hamid-Reza Ahmadi-Ashtiani, Parisa Bishe, Anna Baldisserotto, Piergiacomo Buso, Stefano Manfredini, Silvia Vertuani. Stem Cells as a Target for the Delivery of Active Molecules to Skin by Topical Administration. International Journal of Molecular Sciences. 2020; 21 (6):2251.

Chicago/Turabian Style

Hamid-Reza Ahmadi-Ashtiani; Parisa Bishe; Anna Baldisserotto; Piergiacomo Buso; Stefano Manfredini; Silvia Vertuani. 2020. "Stem Cells as a Target for the Delivery of Active Molecules to Skin by Topical Administration." International Journal of Molecular Sciences 21, no. 6: 2251.

Review
Published: 26 February 2020 in Medicina
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Iran has a rich and diverse cultural heritage, consisting of a complex traditional medicine deeply rooted in the history of the territory that goes back to the Assyrian and Babylonian civilizations. The ethnomedical practices that can be identifiable nowadays derive from the experience of local people who have developed remedies against a wide range of diseases handing down the knowledge from generation to generation over the millennia. Traditional medicine practices represent an important source of inspiration in the process of the development of new drugs and therapeutic strategies. In this context, it is useful to determine the state of the art of ethnomedical studies, concerning the Iranian territory, and of scientific studies on plants used in traditional Iranian medicine. Data regarding 245 plants used in Iranian ethnomedical practices and scientific studies conducted on 89 plants collected in the Iranian territory have been reported. All of the scientific studies here reported draw inspiration from traditional medicine. The World Health Organization (WHO) has repeatedly called for an intensification of the scientific validation processes of traditional medicines intended as an important contribution to public health in various parts of the world. The process of study and validation of Iranian ethnomedical practices appears to be at an early stage.

ACS Style

Piergiacomo Buso; Stefano Manfredini; Hamid Reza Ahmadi-Ashtiani; Sabrina Sciabica; Raissa Buzzi; Silvia Vertuani; Anna Baldisserotto. Iranian Medicinal Plants: From Ethnomedicine to Actual Studies. Medicina 2020, 56, 97 .

AMA Style

Piergiacomo Buso, Stefano Manfredini, Hamid Reza Ahmadi-Ashtiani, Sabrina Sciabica, Raissa Buzzi, Silvia Vertuani, Anna Baldisserotto. Iranian Medicinal Plants: From Ethnomedicine to Actual Studies. Medicina. 2020; 56 (3):97.

Chicago/Turabian Style

Piergiacomo Buso; Stefano Manfredini; Hamid Reza Ahmadi-Ashtiani; Sabrina Sciabica; Raissa Buzzi; Silvia Vertuani; Anna Baldisserotto. 2020. "Iranian Medicinal Plants: From Ethnomedicine to Actual Studies." Medicina 56, no. 3: 97.

Journal article
Published: 28 January 2020 in Nanomaterials
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Skin cancer is the most common malignant cancer with an incidence of 1 million cases/year. It is well known that exposure to UV radiation from sunlight leads the most frequent risk factors for several skin disorders including skin cancer. Sunscreen filters represent a valid protection against dangerous effects derived from UV radiation, and they can be divided in organic and inorganic UV filters. Adding, at the product formulation, molecules with booster effect, or also substances that can increase the protecting effectiveness via synergic mechanisms, can further enhance their protection activity. Moreover, this approach leads to develop formulations with high SPF (Sun Protection Factor) with a reduced content of UV filters, this is in line with the recent decisions of yet a few countries (Palau, Thailand, Philippines, and Hawaii) to ban some sunscreen filters to preserve marine environments (i.e., reef). In this work, a new class of sunscreen UV filters has been synthesized, by means the combination of physical filter and Oxisol, an antioxidant molecule with booster effect. In this study, the synthesis of new physical multifunctional ingredients is reported, by means the direct surface functionalization of inorganic filters (in particular TiO2) with Oxisol. In this study, the full characterization of these multifunctional ingredients is also reported, in addition to the cytotoxicity tests, the photocatalytic activity and the rheological properties involved on skin application.

ACS Style

Mattia Battistin; Valeria Dissette; Alessandro Bonetto; Elisa Durini; Stefano Manfredini; Antonio Marcomini; Elisa Casagrande; Andrea Brunetta; Paola Ziosi; Sonia Molesini; Riccardo Gavioli; Francesco Nicoli; Silvia Vertuani; Anna Baldisserotto. A New Approach to UV Protection by Direct Surface Functionalization of TiO2 with the Antioxidant Polyphenol Dihydroxyphenyl Benzimidazole Carboxylic Acid. Nanomaterials 2020, 10, 231 .

AMA Style

Mattia Battistin, Valeria Dissette, Alessandro Bonetto, Elisa Durini, Stefano Manfredini, Antonio Marcomini, Elisa Casagrande, Andrea Brunetta, Paola Ziosi, Sonia Molesini, Riccardo Gavioli, Francesco Nicoli, Silvia Vertuani, Anna Baldisserotto. A New Approach to UV Protection by Direct Surface Functionalization of TiO2 with the Antioxidant Polyphenol Dihydroxyphenyl Benzimidazole Carboxylic Acid. Nanomaterials. 2020; 10 (2):231.

Chicago/Turabian Style

Mattia Battistin; Valeria Dissette; Alessandro Bonetto; Elisa Durini; Stefano Manfredini; Antonio Marcomini; Elisa Casagrande; Andrea Brunetta; Paola Ziosi; Sonia Molesini; Riccardo Gavioli; Francesco Nicoli; Silvia Vertuani; Anna Baldisserotto. 2020. "A New Approach to UV Protection by Direct Surface Functionalization of TiO2 with the Antioxidant Polyphenol Dihydroxyphenyl Benzimidazole Carboxylic Acid." Nanomaterials 10, no. 2: 231.

Journal article
Published: 12 December 2019 in Molecules
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The incidence of skin cancer is increasing both because of climate change and the increase in pollution than people’s incorrect habits of sun exposure. In these regards, sunscreen and photoprotection are essential tools in consenting the benefits induced by safe solar light exposition and skin cancer prevention. In this work, a new class of sunscreen filter was synthesized by chemical combination of a physical filter (ZnO) and Oxisol (dihydroxyphenyl benzimidazole carboxylic acid), an antioxidant molecule with booster effect. In this work, a new class of filters with new properties was achieved by direct functionalization of particles surface. A full characterization of this multifunctional ingredient (ZnO–Ox) was conducted: Compared with the simple mixture, the new filter acts as a multifunctional molecule showing a higher Sun Protection Factor (SPF), a better cytotoxic profile (MTT and NRU assay), and anti-acne activity. A strong reduction of photocatalytic activity of ZnO was observed, also improving the safety profile.

ACS Style

Mattia Battistin; Elisa Durini; Valeria Dissette; Alessandro Bonetto; Antonio Marcomini; Elisa Casagrande; Andrea Brunetta; Paola Ziosi; Sonia Molesini; Riccardo Gavioli; Francesco Nicoli; Stefano Manfredini; Silvia Vertuani; Anna Baldisserotto. Synthesis and Characterization of New Multifunctional Self-Boosted Filters for UV Protection: ZnO Complex with Dihydroxyphenyl Benzimidazole Carboxylic Acid. Molecules 2019, 24, 4546 .

AMA Style

Mattia Battistin, Elisa Durini, Valeria Dissette, Alessandro Bonetto, Antonio Marcomini, Elisa Casagrande, Andrea Brunetta, Paola Ziosi, Sonia Molesini, Riccardo Gavioli, Francesco Nicoli, Stefano Manfredini, Silvia Vertuani, Anna Baldisserotto. Synthesis and Characterization of New Multifunctional Self-Boosted Filters for UV Protection: ZnO Complex with Dihydroxyphenyl Benzimidazole Carboxylic Acid. Molecules. 2019; 24 (24):4546.

Chicago/Turabian Style

Mattia Battistin; Elisa Durini; Valeria Dissette; Alessandro Bonetto; Antonio Marcomini; Elisa Casagrande; Andrea Brunetta; Paola Ziosi; Sonia Molesini; Riccardo Gavioli; Francesco Nicoli; Stefano Manfredini; Silvia Vertuani; Anna Baldisserotto. 2019. "Synthesis and Characterization of New Multifunctional Self-Boosted Filters for UV Protection: ZnO Complex with Dihydroxyphenyl Benzimidazole Carboxylic Acid." Molecules 24, no. 24: 4546.

Review
Published: 01 November 2019 in Journal of Ethnopharmacology
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The genus Hedyosmum (family: Chloranthaceae) represents an interesting source of natural active compounds, and the 45 species of this genus are widespread in Central and South America and to a lesser extent Southeast Asia (southern China and western Malaysia). Several species are traditionally used in folk medicine. However, the data made available in recent years have not been organized and compared. The present study is a critical assessment of the state-of-the-art concerning the traditional uses, the phytochemistry and the pharmacology of species belonging to the genus Hedyosmum to suggest further research strategies and to facilitate the exploitation of the therapeutic potential of Hedyosmum species for the treatment of human disorders. The present review consists of a systematic overview of scientific literature concerning the genus Hedyosmum published between 1965 and 2018. Moreover, an older text, dated from 1843, concerning the traditional uses of H. bonplandianum Kunth has also been considered. Several databases (Francis & Taylor, Google Scholar, PubMed, SciELO, SciFinder, Springer, Wiley, and The Plant List Database) have been used to perform this work. Sixteen species of the genus Hedyosmum have been mentioned as traditional remedies, and a large number of ethnomedicinal uses, including for the treatment of pain, depression, migraine, stomach-ache and ovary diseases, have been reported. Five species have been used as flavouring agents, tea substitutes or foods. Sesterterpenes, sesquiterpene lactones, monoterpenes, hydroxycinnamic acid derivatives, flavonoids, and neolignans have been reported as the most important compounds in these species. Studies concerning their biological activities have shown that members of the Hedyosmum genus possesses promising biological properties, such as analgesic, antinociceptive, antidepressant, anxiolytic, sedative, and hypnotic effects. Preliminary studies concerning the antibacterial, antioxidant, antiplasmodial, and antifungal activities of these plants as well as their cytotoxic activities against different tumour cell lines have been reported. Some active compounds from the Hedyosmum genus have been used as starting points for the innovative and bioinspired development of synthetic molecules. A critical assessment of these papers has been performed, and some conceptual and methodological problems have been identified regarding the materials and methods and the experimental design used in these studies, including a lack of ethnopharmacological research. The present review partially confirms the basis for some of the traditional uses of Hedyosmum species (mainly H. brasiliense) through preclinical studies that demonstrated their antinociceptive and neuroprotective effects. Due to promising preliminary results, further studies should be conducted on 13-hydroxy-8,9-dehydroshizukanolide and podoandin. Moreover, several essential oils (EOs) from this genus have been preliminarily investigated, and the cytotoxic and antibacterial activities of H. brasiliense and H. sprucei EOs certainly deserve further investigation. From the promising findings of the present analysis, we can affirm that this genus deserves further research from ethnopharmacological and toxicological perspectives.

ACS Style

Matteo Radice; Angélica Tasambay; Amaury Pérez; Karel Diéguez-Santana; Gianni Sacchetti; Piergiacomo Buso; Raissa Buzzi; Silvia Vertuani; Stefano Manfredini; Anna Baldisserotto. Ethnopharmacology, phytochemistry and pharmacology of the genus Hedyosmum (Chlorantaceae): A review. Journal of Ethnopharmacology 2019, 244, 111932 .

AMA Style

Matteo Radice, Angélica Tasambay, Amaury Pérez, Karel Diéguez-Santana, Gianni Sacchetti, Piergiacomo Buso, Raissa Buzzi, Silvia Vertuani, Stefano Manfredini, Anna Baldisserotto. Ethnopharmacology, phytochemistry and pharmacology of the genus Hedyosmum (Chlorantaceae): A review. Journal of Ethnopharmacology. 2019; 244 ():111932.

Chicago/Turabian Style

Matteo Radice; Angélica Tasambay; Amaury Pérez; Karel Diéguez-Santana; Gianni Sacchetti; Piergiacomo Buso; Raissa Buzzi; Silvia Vertuani; Stefano Manfredini; Anna Baldisserotto. 2019. "Ethnopharmacology, phytochemistry and pharmacology of the genus Hedyosmum (Chlorantaceae): A review." Journal of Ethnopharmacology 244, no. : 111932.

Journal article
Published: 25 July 2019 in Toxins
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Oleander (Nerium oleander) is an ornamental plant common in tropical and sub-tropical regions that is becoming increasingly widespread, even in temperate regions. Oleander poisoning may occur in animals and humans. The main active components contained in the plant are cardiac glycosides belonging to the class of cardenolides that are toxic to many species, from human to insects. This work describes a case of oleander poisoning that occurred on a small cattle farm and resulted in the fatality of all six resident animals. Furthermore, the investigation of the poisonous agent is described, with particular focus on the characterization of the oleandrin toxin that was recovered from the forage and rumen contents. The innovation of this study is the first description of the detection and quantification of the oleandrin toxin by liquid chromatography-high resolution mass spectrometry (LC-HRMS) in rumen.

ACS Style

Silva Rubini; Sabina Strano Rossi; Serena Mestria; Sara Odoardi; Sara Chendi; Andrea Poli; Giuseppe Merialdi; Giuseppina Andreoli; Paolo Frisoni; Rosa Maria Gaudio; Anna Baldisserotto; Piergiacomo Buso; Stefano Manfredini; Guido Govoni; Stefania Barbieri; Cinzia Centelleghe; Giorgia Corazzola; Sandro Mazzariol; Carlo Alessandro Locatelli; Poli; Buso. A Probable Fatal Case of Oleander (Nerium oleander) Poisoning on a Cattle Farm: A New Method of Detection and Quantification of the Oleandrin Toxin in Rumen. Toxins 2019, 11, 442 .

AMA Style

Silva Rubini, Sabina Strano Rossi, Serena Mestria, Sara Odoardi, Sara Chendi, Andrea Poli, Giuseppe Merialdi, Giuseppina Andreoli, Paolo Frisoni, Rosa Maria Gaudio, Anna Baldisserotto, Piergiacomo Buso, Stefano Manfredini, Guido Govoni, Stefania Barbieri, Cinzia Centelleghe, Giorgia Corazzola, Sandro Mazzariol, Carlo Alessandro Locatelli, Poli, Buso. A Probable Fatal Case of Oleander (Nerium oleander) Poisoning on a Cattle Farm: A New Method of Detection and Quantification of the Oleandrin Toxin in Rumen. Toxins. 2019; 11 (8):442.

Chicago/Turabian Style

Silva Rubini; Sabina Strano Rossi; Serena Mestria; Sara Odoardi; Sara Chendi; Andrea Poli; Giuseppe Merialdi; Giuseppina Andreoli; Paolo Frisoni; Rosa Maria Gaudio; Anna Baldisserotto; Piergiacomo Buso; Stefano Manfredini; Guido Govoni; Stefania Barbieri; Cinzia Centelleghe; Giorgia Corazzola; Sandro Mazzariol; Carlo Alessandro Locatelli; Poli; Buso. 2019. "A Probable Fatal Case of Oleander (Nerium oleander) Poisoning on a Cattle Farm: A New Method of Detection and Quantification of the Oleandrin Toxin in Rumen." Toxins 11, no. 8: 442.

Journal article
Published: 25 April 2019 in Molecules
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The chemical composition and biological activity of essential oils isolated from the leaves of Siparuna aspera, Siparuna macrotepala, Piper leticianum, Piper augustum and the rhizome of Hedychium coronarium were evaluated. These species are used medicinally in different ways by the Amazonian communities that live near the Kutukú mountain range. Chemical studies revealed that the main components for the two Siparuna species were germacrene D, bicyclogermacrene, α-pinene, δ-cadinene, δ-elemene, α-copaene and β-caryophyllene; for the two Piper species β-caryophyllene, germacrene D, α-(E,E)-farnesene, β-elemene, bicyclogermacrene, δ-cadinene and for H. coronarium 1,8-cineole, β-pinene, α-pinene and α-terpineol. The antioxidant activity of all essential oils was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), photochemiluminescence (PCL) quantitative assays, and DPPH and ABTS bioautographic profiles, with different results for each of them. Antimicrobial activity studies were carried out on three yeasts, six Gram positive and four Gram negative bacteria, by means of the disc diffusion method. The essential oil of H. coronarium showed the most relevant results on L. grayi, K. oxytoca and S. mutans, P. augustum and P. leticianum on S. mutans. An antibacterial bioautographic test for H. coronarium was also carried out and highlighted the potential activity of terpinen-4-ol and 1,8-cineole.

ACS Style

Paco Noriega; Alessandra Guerrini; Gianni Sacchetti; Alessandro Grandini; Edwin Ankuash; Stefano Manfredini. Chemical Composition and Biological Activity of Five Essential Oils from the Ecuadorian Amazon Rain Forest. Molecules 2019, 24, 1637 .

AMA Style

Paco Noriega, Alessandra Guerrini, Gianni Sacchetti, Alessandro Grandini, Edwin Ankuash, Stefano Manfredini. Chemical Composition and Biological Activity of Five Essential Oils from the Ecuadorian Amazon Rain Forest. Molecules. 2019; 24 (8):1637.

Chicago/Turabian Style

Paco Noriega; Alessandra Guerrini; Gianni Sacchetti; Alessandro Grandini; Edwin Ankuash; Stefano Manfredini. 2019. "Chemical Composition and Biological Activity of Five Essential Oils from the Ecuadorian Amazon Rain Forest." Molecules 24, no. 8: 1637.

Journal article
Published: 03 March 2019 in Molecules
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Biofilm resistance to antimicrobials is a complex phenomenon, driven not only by genetic mutation induced resistance, but also by means of increased microbial cell density that supports horizontal gene transfer across cells. The prevention of biofilm formation and the treatment of existing biofilms is currently a difficult challenge; therefore, the discovery of new multi-targeted or combinatorial therapies is growing. The development of anti-biofilm agents is considered of major interest and represents a key strategy as non-biocidal molecules are highly valuable to avoid the rapid appearance of escape mutants. Among bacteria, staphylococci are predominant causes of biofilm-associated infections. Staphylococci, especially Staphylococcus aureus (S. aureus) is an extraordinarily versatile pathogen that can survive in hostile environmental conditions, colonize mucous membranes and skin, and can cause severe, non-purulent, toxin-mediated diseases or invasive pyogenic infections in humans. Staphylococcus epidermidis (S. epidermidis) has also emerged as an important opportunistic pathogen in infections associated with medical devices (such as urinary and intravascular catheters, orthopaedic implants, etc.), causing approximately from 30% to 43% of joint prosthesis infections. The scientific community is continuously looking for new agents endowed of anti-biofilm capabilities to fight S. aureus and S epidermidis infections. Interestingly, several reports indicated in vitro efficacy of non-biocidal essential oils (EOs) as promising treatment to reduce bacterial biofilm production and prevent the inducing of drug resistance. In this report were analyzed 89 EOs with the objective of investigating their ability to modulate bacterial biofilm production of different S. aureus and S. epidermidis strains. Results showed the assayed EOs to modulated the biofilm production with unpredictable results for each strain. In particular, many EOs acted mainly as biofilm inhibitors in the case of S. epidermidis strains, while for S. aureus strains, EOs induced either no effect or stimulate biofilm production. In order to elucidate the obtained experimental results, machine learning (ML) algorithms were applied to the EOs’ chemical compositions and the determined associated anti-biofilm potencies. Statistically robust ML models were developed, and their analysis in term of feature importance and partial dependence plots led to indicating those chemical components mainly responsible for biofilm production, inhibition or stimulation for each studied strain, respectively.

ACS Style

Alexandros Patsilinakos; Marco Artini; Rosanna Papa; Manuela Sabatino; Mijat Božović; Stefania Garzoli; Gianluca Vrenna; Raissa Buzzi; Stefano Manfredini; Laura Selan; Rino Ragno. Machine Learning Analyses on Data including Essential Oil Chemical Composition and In Vitro Experimental Antibiofilm Activities against Staphylococcus Species. Molecules 2019, 24, 890 .

AMA Style

Alexandros Patsilinakos, Marco Artini, Rosanna Papa, Manuela Sabatino, Mijat Božović, Stefania Garzoli, Gianluca Vrenna, Raissa Buzzi, Stefano Manfredini, Laura Selan, Rino Ragno. Machine Learning Analyses on Data including Essential Oil Chemical Composition and In Vitro Experimental Antibiofilm Activities against Staphylococcus Species. Molecules. 2019; 24 (5):890.

Chicago/Turabian Style

Alexandros Patsilinakos; Marco Artini; Rosanna Papa; Manuela Sabatino; Mijat Božović; Stefania Garzoli; Gianluca Vrenna; Raissa Buzzi; Stefano Manfredini; Laura Selan; Rino Ragno. 2019. "Machine Learning Analyses on Data including Essential Oil Chemical Composition and In Vitro Experimental Antibiofilm Activities against Staphylococcus Species." Molecules 24, no. 5: 890.

Journal article
Published: 16 January 2019 in International Journal of Pharmaceutics
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An innovative lyophilized dry powder formulation consisting of urea-crosslinked hyaluronic acid (HA–CL) and sodium ascorbyl phosphate (SAP) -LYO HA–CL – SAP- was prepared and characterized in vitro for physico-chemical and biological properties. The aim was to understand if LYO HA–CL – SAP could be used as adjuvant treatment for nasal inflammatory diseases. LYO HA–CL – SAP was suitable for nasal delivery and showed to be not toxic on human nasal septum carcinoma-derived cells (RPMI 2650 cells) at the investigated concentrations. It displayed porous, polygonal particles with unimodal, narrow size distribution, mean geometric diameter of 328.3 ± 27.5 µm, that is appropriate for nasal deposition with no respirable fraction and 88.7% of particles with aerodynamic diameter > 14.1 µm. Additionally, the formulation showed wound healing ability on RPMI 2650 cells, and reduced interleukin–8 (IL–8) level in primary nasal epithelial cells pre-induced with lipopolysaccharide (LPS). Transport study across RPMI 2650 cells showed that HA–CL could act not only as carrier for SAP and active ingredient itself, but potentially also as mucoadhesive agent. In conclusion, these results suggest that HA–CL and SAP had anti-inflammatory activity and acted in combination to accelerate wound healing. Therefore, LYO HA–CL – SAP could be a potential adjuvant in nasal anti-inflammatory formulations.

ACS Style

Arianna Fallacara; Laura Busato; Michele Pozzoli; Maliheh Ghadiri; Hui Xin Ong; Paul Young; Stefano Manfredini; Daniela Traini. In vitro characterization of physico-chemical properties, cytotoxicity, bioactivity of urea-crosslinked hyaluronic acid and sodium ascorbyl phosphate nasal powder formulation. International Journal of Pharmaceutics 2019, 558, 341 -350.

AMA Style

Arianna Fallacara, Laura Busato, Michele Pozzoli, Maliheh Ghadiri, Hui Xin Ong, Paul Young, Stefano Manfredini, Daniela Traini. In vitro characterization of physico-chemical properties, cytotoxicity, bioactivity of urea-crosslinked hyaluronic acid and sodium ascorbyl phosphate nasal powder formulation. International Journal of Pharmaceutics. 2019; 558 ():341-350.

Chicago/Turabian Style

Arianna Fallacara; Laura Busato; Michele Pozzoli; Maliheh Ghadiri; Hui Xin Ong; Paul Young; Stefano Manfredini; Daniela Traini. 2019. "In vitro characterization of physico-chemical properties, cytotoxicity, bioactivity of urea-crosslinked hyaluronic acid and sodium ascorbyl phosphate nasal powder formulation." International Journal of Pharmaceutics 558, no. : 341-350.

Journal article
Published: 15 December 2018 in Cosmetics
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Hesperidin is one of the most important natural flavonoids, known for its antioxidant, anti-inflammatory, anti-mutagenic, and anti-hypertensive properties. Despite its various biological activities, hesperidin is rarely used in the dermo-cosmetic field because of its poor solubility in both water and oil phases that makes difficult formulation, distribution and bioavailability through the skin layers. Moreover, hesperidin is still underestimated in skin care products, and literature data on its stability into a topical formulation are not yet available. In this paper we report the synthesis of five different derivatives of hesperidin and their evaluation in terms of antioxidant, antifungal, antiproliferative, and apoptotic effects on human leukemic K562 cells. Preliminary antiproliferative effects were considered since hyper-proliferation is involved in several cutaneous problems particularly in the case of photo-exposition and environmental pollution. Esp4 and Esp5 were found to be more active in inhibiting K562 cell growth than parent hesperidin. Esp3 exhibited different biological properties, i.e., antioxidant activity in the absence of antiproliferative effects.

ACS Style

Alessia Bino; Chiara Beatrice Vicentini; Silvia Vertuani; Ilaria Lampronti; Roberto Gambari; Elisa Durini; Stefano Manfredini; Anna Baldisserotto. Novel Lipidized Derivatives of the Bioflavonoid Hesperidin: Dermatological, Cosmetic and Chemopreventive Applications. Cosmetics 2018, 5, 72 .

AMA Style

Alessia Bino, Chiara Beatrice Vicentini, Silvia Vertuani, Ilaria Lampronti, Roberto Gambari, Elisa Durini, Stefano Manfredini, Anna Baldisserotto. Novel Lipidized Derivatives of the Bioflavonoid Hesperidin: Dermatological, Cosmetic and Chemopreventive Applications. Cosmetics. 2018; 5 (4):72.

Chicago/Turabian Style

Alessia Bino; Chiara Beatrice Vicentini; Silvia Vertuani; Ilaria Lampronti; Roberto Gambari; Elisa Durini; Stefano Manfredini; Anna Baldisserotto. 2018. "Novel Lipidized Derivatives of the Bioflavonoid Hesperidin: Dermatological, Cosmetic and Chemopreventive Applications." Cosmetics 5, no. 4: 72.

Journal article
Published: 01 December 2018 in Pharmaceutics
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The present work evaluates for the first time the use of urea-crosslinked hyaluronic acid (HA-CL), a novel derivative of native hyaluronic acid (HA), to produce microspheres (MS) by emulsification-solvent evaporation, for dermal delivery of sodium ascorbyl phosphate (SAP). As the term of comparison, HA MS were prepared. A pre-formulation study—investigation of the effects of polymers solutions properties (pH, viscosity) and working conditions—led to the - production of optimized HA-CL MS and HA-CL—SAP MS with: almost unimodal size distributions; mean diameter of 13.0 ± 0.7 and 9.9 ± 0.8 µm, respectively; spherical shape and rough surface; high yield, similar to HA MS and HA–SAP MS (≈ 85%). SAP was more efficiently encapsulated into HA-CL MS (78.8 ± 2.6%) compared to HA MS (69.7 ± 4.6%). Physical state, thermal properties, relative moisture stability of HA-CL MS and HA-CL–SAP MS were comparable to those of HA MS and HA–SAP MS. However, HA-CL–SAP MS exhibited an extended drug release compared to HA–SAP MS, despite the same kinetic mechanism—contemporaneous drug diffusion and polymer swelling/dissolution. Therefore, HA-CL formulation showed a greater potential as microcarrier (for encapsulation efficiency and release kinetic), that could be improved, in future, using suitable excipients.

ACS Style

Arianna Fallacara; Filippo Marchetti; Michele Pozzoli; Ugo Raffaello Citernesi; Stefano Manfredini; And Silvia Vertuani; Silvia Vertuani. Formulation and Characterization of Native and Crosslinked Hyaluronic Acid Microspheres for Dermal Delivery of Sodium Ascorbyl Phosphate: A Comparative Study. Pharmaceutics 2018, 10, 254 .

AMA Style

Arianna Fallacara, Filippo Marchetti, Michele Pozzoli, Ugo Raffaello Citernesi, Stefano Manfredini, And Silvia Vertuani, Silvia Vertuani. Formulation and Characterization of Native and Crosslinked Hyaluronic Acid Microspheres for Dermal Delivery of Sodium Ascorbyl Phosphate: A Comparative Study. Pharmaceutics. 2018; 10 (4):254.

Chicago/Turabian Style

Arianna Fallacara; Filippo Marchetti; Michele Pozzoli; Ugo Raffaello Citernesi; Stefano Manfredini; And Silvia Vertuani; Silvia Vertuani. 2018. "Formulation and Characterization of Native and Crosslinked Hyaluronic Acid Microspheres for Dermal Delivery of Sodium Ascorbyl Phosphate: A Comparative Study." Pharmaceutics 10, no. 4: 254.

Journal article
Published: 14 November 2018 in Cosmetics
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Carnosine has several physiological roles, from intracellular pH buffering to antioxidant activities, which all depend on bioavailability. This study was conducted in a human skin 3D model and focuses on the effects of the topical delivery of carnosine, from a dermo-cosmetic gel, through the stratum corneum in the presence of a magnesium ion as a complexing agent. To evaluate possible enhancement for small peptide delivery to the skin from simple cosmetic formulations, we discovered that complexation was able to improve the delivery of carnosine into human skin 3D models by application in gel formulation. The concentrations of carnosine released in the underlying media and those that remained in the reconstructed human epidermis (RHE) tissues after 24 and 48 h exposure were measured. Moreover, the influence of magnesium ions was also evaluated comparing the same formulation with and without the salt. The results obtained in this study support hypothesis that magnesium can influence the delivery of small peptides and that the gel formulation based on the carnosine-magnesium complex allows for superior delivery of carnosine in the lower skin layer at a concentration up to 60% more than carnosine alone.

ACS Style

Valeria Dissette; Carlo Alberto Bignozzi; Giuseppe Valacchi; Alessandra Pecorelli; Stefano Manfredini; Silvia Vertuani. Evaluation of the Transepidermal Penetration of a Carnosine Complex in Gel Formulation by 3D Skin Models. Cosmetics 2018, 5, 67 .

AMA Style

Valeria Dissette, Carlo Alberto Bignozzi, Giuseppe Valacchi, Alessandra Pecorelli, Stefano Manfredini, Silvia Vertuani. Evaluation of the Transepidermal Penetration of a Carnosine Complex in Gel Formulation by 3D Skin Models. Cosmetics. 2018; 5 (4):67.

Chicago/Turabian Style

Valeria Dissette; Carlo Alberto Bignozzi; Giuseppe Valacchi; Alessandra Pecorelli; Stefano Manfredini; Silvia Vertuani. 2018. "Evaluation of the Transepidermal Penetration of a Carnosine Complex in Gel Formulation by 3D Skin Models." Cosmetics 5, no. 4: 67.

Research article
Published: 11 November 2018 in Oxidative Medicine and Cellular Longevity
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Red blood cells (RBCs) from people affected by autism spectrum disorders (ASDs) are a target of oxidative stress. By scanning electron microscopy, we analyzed RBC morphology from 22 ASD children and show here that only 47.5 ± 3.33% of RBC displayed the typical biconcave shape, as opposed to 87.5 ± 1.3% (mean ± SD) of RBC from 21 sex- and age-matched healthy typically developing (TD) controls. Codocytes and star-shaped cells accounted for about 30% of all abnormally shaped ASD erythrocytes. RBC shape alterations were independent of the anticoagulant used (Na2-EDTA or heparin) and of different handling procedures preceding glutaraldehyde fixation, thus suggesting that they were not artefactual. Incubation for 24 h in the presence of antioxidants restored normal morphology in most erythrocytes from ASD patients. By Coomassie staining, as well as Western blotting analysis of relevant proteins playing a key role in the membrane-cytoskeleton organization, we were unable to find differences in RBC ghost composition between ASD and normal subjects. Phosphatidylserine (PS) exposure towards the extracellular membrane domain was examined in both basal and erythroptosis-inducing conditions. No differences were found between ASD and TD samples except when the aminophospholipid translocase was blocked by N-ethylmaleimide, upon which an increased amount of PS was found to face the outer membrane in RBC from ASD. These complex data are discussed in the light of the current understanding of the mode by which oxidative stress might affect erythrocyte shape in ASD and in other pathological conditions.

ACS Style

Alessandra Bolotta; Michela Battistelli; Elisabetta Falcieri; Alessandro Ghezzo; Maria Cristina Manara; Stefano Manfredini; Marina Marini; Annio Posar; Paola Visconti; Provvidenza Maria Abruzzo. Oxidative Stress in Autistic Children Alters Erythrocyte Shape in the Absence of Quantitative Protein Alterations and of Loss of Membrane Phospholipid Asymmetry. Oxidative Medicine and Cellular Longevity 2018, 2018, 1 -11.

AMA Style

Alessandra Bolotta, Michela Battistelli, Elisabetta Falcieri, Alessandro Ghezzo, Maria Cristina Manara, Stefano Manfredini, Marina Marini, Annio Posar, Paola Visconti, Provvidenza Maria Abruzzo. Oxidative Stress in Autistic Children Alters Erythrocyte Shape in the Absence of Quantitative Protein Alterations and of Loss of Membrane Phospholipid Asymmetry. Oxidative Medicine and Cellular Longevity. 2018; 2018 ():1-11.

Chicago/Turabian Style

Alessandra Bolotta; Michela Battistelli; Elisabetta Falcieri; Alessandro Ghezzo; Maria Cristina Manara; Stefano Manfredini; Marina Marini; Annio Posar; Paola Visconti; Provvidenza Maria Abruzzo. 2018. "Oxidative Stress in Autistic Children Alters Erythrocyte Shape in the Absence of Quantitative Protein Alterations and of Loss of Membrane Phospholipid Asymmetry." Oxidative Medicine and Cellular Longevity 2018, no. : 1-11.

Journal article
Published: 07 November 2018 in Cosmetics
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Several pathologies are characterized by chronic wounds and often resistant to many of the common therapies, leading to chronic infections that can become even life-threatening for patients. For this reason, the identification of new products able to ameliorate the healing process is still an on-going research. Natural compounds have been used to improve skin conditions due to their dermo-cosmetic and therapeutic activities including anti-inflammatory, antioxidant and cell-migratory properties. Among these compounds, it has been recently demonstrated that Verbascoside, a phenyl propanoid glycoside widely used in the cosmetic field, can improve keratinocytes proliferation. Because of its high hydrophilic character, Verbascoside has a limited range of possible topical applications and the synthesis of ES2, a semi-synthetic derivative of Verbascoside was performed to bypass some of the drawback aspects of this molecule. In the present study, the wound healing properties of Verbascoside and ES2 were compared in both keratinocytes “in vitro” wound scratch and in wounded SKH1 mice. The results showed that both compounds were not cytotoxic and ES2 showed an efficient ability to promote the proliferation of human keratinocytes compared to Verbascoside. The findings were also confirmed in vivo but only at early time points (2/3 days). Taken together, these data suggest that the Verbascoside-derivative ES2 could be considered a novel and promising candidate for the topical treatment of wounds.

ACS Style

Ilaria Crivellari; Silvia Vertuani; Yunsook Lim; Franco Cervellati; Anna Baldisserotto; Stefano Manfredini; Giuseppe Valacchi. ES2 as a Novel Verbascoside-Derived Compound in the Treatment of Cutaneous Wound Healing. Cosmetics 2018, 5, 65 .

AMA Style

Ilaria Crivellari, Silvia Vertuani, Yunsook Lim, Franco Cervellati, Anna Baldisserotto, Stefano Manfredini, Giuseppe Valacchi. ES2 as a Novel Verbascoside-Derived Compound in the Treatment of Cutaneous Wound Healing. Cosmetics. 2018; 5 (4):65.

Chicago/Turabian Style

Ilaria Crivellari; Silvia Vertuani; Yunsook Lim; Franco Cervellati; Anna Baldisserotto; Stefano Manfredini; Giuseppe Valacchi. 2018. "ES2 as a Novel Verbascoside-Derived Compound in the Treatment of Cutaneous Wound Healing." Cosmetics 5, no. 4: 65.