Purpose: Numerous carbohydrate polymers are frequently used in wound-dressing films because they are highly effective materials for promoting successful wound healing. In this study, we prepared amikacin (AM)-containing hydrogel films through the cross-linking of chitosan (CS) with folic acid along with methacrylic acid (MA), ammonium peroxodisulfate (APS), and methylenebisacrylamide (MBA). In the current studies, an effort has been made to look at the possibilities of these materials in developing new hydrogel film wound dressings meant for a slow release of the antibiotic AM and to enhance the potential for wound healing. Methods: Free-radical polymerization was used to generate the hydrogel film, and different concentrations of the CS polymer were used. Measurements were taken of the film thickness, weight fluctuation, folding resistance, moisture content, and moisture uptake. HPLC, FTIR, SEM, DSC, and AFM analyses were some of the different techniques used to confirm that the films were successfully developed. Results: The AM release profile demonstrated regulated release over a period of 24 h in simulated wound media at pH 5.5 and 7.4, with a low initial burst release. The antibacterial activity against gram-negative bacterial strains exhibited substantial effectiveness, with inhibitory zones measuring approximately 20.5 ± 0.1 mm. Additionally, in vitro cytocompatibility assessments demonstrated remarkable cell viability, surpassing 80%, specifically when evaluated against human skin fibroblast (HFF-1) cells. Conclusions: The exciting findings of this study indicate the promising potential for further development and testing of these hydrogel films, offering effective and controlled antibiotic release to enhance the process of wound healing.

In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. The analyte was quantified by competitive detection with SA2BSA immobilized on the WE toward a mixture of Ab155 antibody (with fixed concentration) and sulfapyridine. In order to obtain a highly sensitive biosensor, Ab155 was immobilized onto magnetic latex nanoparticles surface to create a 3D architecture (Ab-MLNp). Using electrochemical impedance spectroscopy (EIS), we investigated the influence of the Ab-MLNp on the sensitivity of our approach. The optimized system was analyzed, as competitive assay, with different concentrations of sulfapyridine (40 µM, 4 µM, and 2 nM) and with phosphate buffer solution. From data fitting calculations and graphs, it was observed that the EIS showed more linearity when Ab-MLNp was used. This result indicates that the magnetic latex nanoparticles increased the sensitivity of the biosensor.

Background: Gradual liver damage occurs due to hepatitis C virus (HCV) that may result in liver fibrosis and even cancer. Pakistan is a developing country with the second highest prevalence 4.5-8% of hepatitis C. The main purpose of this study was to estimate the quality of life of people affected with hepatitis, to create general awareness about the disease and to determine pharmacoeconomic considerations of hepatitis C treatment. Methods: It was a cross-sectional study conducted in village Wali Pur Bura of District Nankana sahib, Punjab, Pakistan. Sample size determined by Daniels equation was 100, both male and female participants were included this study. The data was collected to assess the health status data of study population through validated questionnaire and a urdu version of tool EuroQol 5D-5L, comprising of five (5) questions covering the various aspects of health. Data were analyzed through SPSS version 16. Paired sample statistics for comparing two attributes of the study and frequencies were calculated. Results: In current study, majority (70 %) of patients were belong to the lower class and only 39% patients were aware of Punjab Government's participation in hepatitis free treatment plan. 60% patients showed marked reduction in quality of life and the reason behind was painful interferon therapy. Poor quality of life is observed due to the extra hepatic symptoms of HCV including depression. According to the health scale of 60 participants out of 100 was having below Average health status, 21 participants possessed Average Health while 19 participants had Excellent Health. Conclusion: Hence, concluded that due to hepatitis C, health as well as socioeconomic and family life is badly affected. Majority of the participants were having poor quality of life. Costly treatment is beyond the reach of majority of rural population that increases the incidence of depression among patients and their families.

Synthèse de latex magnétique submicronique et fonctionnalisé pour application en biocapteur: L'objectif de ce travail est de surmonter tous ces processus long et fastidieux comme la filtration et la centrifugation qui sont utilisées dans l'application in-vitro. En plus, ces particules qui sont appropriés pour une utilisation dans le laboratoire-sur une-puce et les biocapteurs systèmes sont produites. Les particules submicroniques magnétiques de latex (MLPs) avec la morphologie noyaucoque souhaité ont été préparées. L'huile dans eau (h/e) et l'émulsion magnétique (faitmaison) a été utilisé en tant que graine de polymérisation radicalaire en émulsion de styrène (St) en tant que monomère et agent de reticulation divenylbenzene (DVB) en présence de persulfate de potassium qu'initiateur. Les particules de la surface ont été fonctionnalisés avec du sulfate et des groupes carboxyliques en utilisant les initiateurs. Un nouveau biocapteur électrochimique de capacité basé sur de substrat de nitrure de silicium (Si3N4) combiné à des MLPs a été développé. MLPs avec terminaison acide carboxylique ont été liés de manière covalente à Si3N4 à travers des monocouches autoassemblées (SAMs) du silane-amine (3-aminopropyl) triéthoxysilane (APTES). Enfin les anticorps anti-ochratoxine A ont été immobilisés sur les MLPs par liaison amide. Mesures électrochimiques ont été effectuées en utilisant une analyse Mott-Schottky pour la détection de l'ochratoxine A (OTA). L'utilisation de l'application de dosage compétitif grà¢ce à l'immobilisation des quantités fixe d'antigène (SA2BSA) et d'anticorps (Ab 155) a été mesurée respectivement contre différentes concentrations de sulfamides pyridine (SPY), avec et sans utilisation de MLPs avec une fonctionnalité de groupe carboxylique. La sensibilité de Biocapteur a augmenté quand MLPs ont été utilisés