The obligate hemiparasite Striga hermonthica is one of the major global biotic threats to agriculture in sub-Saharan Africa, causing severe yield losses of cereals. The germination of Striga seeds relies on host-released signaling molecules, mainly strigolactones (SLs). This dependency opens up the possibility of deploying SL analogs as “suicidal germination agents” to reduce the accumulated seed bank of Striga in infested soils. Although several synthetic SL analogs have been developed for this purpose, the utility of these compounds in realizing the suicidal germination strategy for combating Striga is still largely unknown. Here, we evaluated the efficacy of three potent SL analogs (MP3, MP16, and Nijmegen-1) under laboratory, greenhouse, and farmer’s field conditions. All investigated analogs showed around a 50% Striga germination rate, equivalent to a 50% reduction in infestation, which was comparable to the standard SL analog GR24. Importantly, MP16 had the maximum reduction of Striga emergence (97%) in the greenhouse experiment, while Nijmegen-1 appeared to be a promising candidate under field conditions, with a 43% and 60% reduction of Striga emergence in pearl millet and sorghum fields, respectively. These findings confirm that the selected SL analogs appear to make promising candidates as simple suicidal agents both under laboratory and real African field conditions, which may support us to improve suicidal germination technology to deplete the Striga seed bank in African agriculture.

Resilience of smallholder farmers in their ability to bounce-back and overcome shocks, such as drought, is critical to ensure a pathway out of hunger and poverty. Efficient water conservation methods that increase rainwater capture and reduce soil erosion such as stone lines and grass bands are two technologies that have been proposed to increase the resilience in Sudano–Sahelian farming systems. In Burkina Faso, we show that stone lines, grass bands, and crop rotation are effective resilience strategies individually and in combination. During years when rainfall is well-distributed over time, differences are minimal between fields with water conservation methods and fields without. However, when there are periods of prolonged drought, water conservation methods are effective for increasing soil water, yield, revenue, and resilience. During drought conditions, sorghum (Sorghum bicolor (L.) Moench) grain yield and revenue with stone lines and grass bands were over 50% greater than that of the control, by an average of 450 kg ha⁻¹, which amounted to an increase of 58,500 West African CFA franc (CFA) ha⁻¹ (i.e., 98 USD ha⁻¹). The results also suggest that the combination of water conservation method and crop rotation additionally improves cropping system productivity and revenue. Growing cowpea (Vigna unguiculata (L.) Walp.) in rotation with sorghum production provided more options for farmers to increase their income and access to nutrition. This study also sheds light on the limited productivity gains due to improved crop varieties. The local sorghum landrace, Nongomsoba, and the local cowpea variety in rotation resulted in the highest yields as compared to the improved varieties of Sariaso 14 sorghum and KVX 396-4-4 cowpea. Under similar low input/degraded conditions, improved crop varieties likely are not a suitable resilience strategy alone. We conclude that during erratic rainy seasons with frequent periods of drought (i.e., water stress) in rain-fed conditions in Burkina Faso, stone lines or grass bands in combination with sorghum and cowpea rotation are effective practices for increasing resilience of smallholder farmers to maintain crop productivity and revenue. With future and present increases in climate variability due to climate change, stone lines, grass bands, and crop rotation will have growing importance as resilience strategies to buffer crop productivity and revenue during periods of drought.

Striga hermonthica, a member of the Orobanchaceae family, is an obligate root parasite of staple cereal crops, which poses a tremendous threat to food security, contributing to malnutrition and poverty in many African countries. Depleting Striga seed reservoirs from infested soils is one of the crucial approaches to minimize subterranean damage to crops. The dependency of Striga germination on the host-released strigolactones (SLs) has prompted the development of the “Suicidal Germination” strategy to reduce the accumulated seed bank of Striga. The success of aforementioned strategy depends not only on the activity of the applied SL analogs, but also requires suitable application protocol with simple, efficient, and handy formulation for rain-fed African agriculture. Here, we developed a new formulation “Emulsifiable Concentration (EC)” for the two previously field-assessed SL analogs Methyl phenlactonoate 3 (MP3) and Nijmegen-1. The new EC formulation was evaluated for biological activities under lab, greenhouse, mini-field, and field conditions in comparison to the previously used Atlas G-1086 formulation. The EC formulation of SL analogs showed better activities on Striga germination with lower EC₅₀ and high stability under Lab conditions. Moreover, EC formulated SL analogs at 1.0 µM concentrations reduced 89–99% Striga emergence in greenhouse. The two EC formulated SL analogs showed also a considerable reduction in Striga emergence in mini-field and field experiments. In conclusion, we have successfully developed a desired formulation for applying SL analogs as suicidal agents for large-scale field application. The encouraging results presented in this study pave the way for integrating the suicidal germination approach in sustainable Striga management strategies for African agriculture.

Prospects for an integrated control of Loranthaceae species parasitizing Vitellaria paradoxa C. F. Gaertn in Burkina Faso

Integrated control of Striga hermonthica (Del.) Benth. in Burkina Faso through host plant resistance, biocontrol and fertilizers