Ratoon rice can improve rice yield by increasing the multiple cropping index in China. However, the greenhouse gas (CH₄ and N₂O) emission characteristics from ratoon rice fields and the cultivation methods to reduce CH₄ and N₂O emissions are rarely reported. This study first conducted the analysis of genotype differences in greenhouse gas emission fluxes using five strong ratoon ability rice varieties in 2020. Second, water management methods, including alternating the wet–dry irrigation (AWD) pattern and conventional flooding irrigation (CF) during the main season, were carried out in 2021. CH₄ and N₂O emission flux, agronomic traits, and rice yield during both main and ratoon seasons were investigated. The results showed that the CH₄ emission flux during the main and ratoon seasons was 157.05–470.73 kg·ha^–1 and 31.03–84.38 kg·ha^–1, respectively, and the total N₂O emission flux was 0.13–0.94 kg·ha^–1 in the ratoon rice system over the two seasons (RRSTS). Compared with the main season, the CH₄ emission flux during the ratoon season was significantly reduced, thus decreasing the greenhouse gas global warming potential (GWP) and greenhouse gas emission intensity (GHGI) in the ratoon rice system. Cliangyouhuazhan (CLYHZ) showed a high yield, and the lowest GWP and GHGI values among the five rice varieties in RRSTS. Compared with CF, the AWD pattern reduced the CH₄ emission flux during the main and ratoon seasons by 67.4–95.3 kg·ha^–1 and 1.7–5.1 kg·ha^–1, respectively, but increased the N₂O emission flux by 0.1–0.6 kg·ha^–1 during the RRSTS. Further, compared with CF, the AWD pattern had a declined GWP by 14.3–19.4% and GHGI by 30.3–34.3% during the RRSTS, which was attributed to the significant reduction in GWP and GHGI during the main season. The AWD pattern significantly increased rice yield by 21.9–22.9% during the RRSTS, especially for YX203. Correlation analysis showed that CH₄, GWP, and GHGI exhibited significant negative correlations with spikelet number per m² and the harvest index during the main and ratoon seasons. Collectively, selecting the high-yield, low-emission variety CLYHZ could significantly reduce greenhouse gas emissions from ratoon rice while maintaining a high yield. The AWD pattern could reduce total CH₄ emission during the main season, reducing the GWP and GHGI while increasing the ratoon rice system yield. It could be concluded that a variety of CLYHZ and AWD patterns are worthy of promotion and application to decrease greenhouse gas emissions in the ratoon rice area in the upper reaches of Yangtze River, China.

Biochar is beneficial as a clean, stable, and efficient soil amendment to improve rice quality and yield. However, there are few reports on the effects of no-tillage in combination with biochar application on rice growth, yield, and quality in regenerative rice systems. This study evaluated rice yield, grain quality, multiple antioxidant enzyme activities, and malondialdehyde content under four treatments: rotary tillage alone, rotary tillage + biochar application, no-tillage alone, and no-tillage + biochar. The results showed that the rice yield under no-tillage alone was 15% lower than that under rotary tillage alone, but that biochar application significantly increased rice yield by 10% and 20% under rotary tillage and no-tillage conditions, respectively, which might be attributed to the fact that biochar application increased panicle number, spikelet number per panicle, grain filling rate, and antioxidant enzyme activities. Additionally, biochar application also increased fine rice rate and protein content, meanwhile reducing chalkiness degree and chalky grain rate in both the main-season rice and ratoon-season rice. These results suggest that biochar application could enhance the yield and grain quality of ratoon rice, thus compensating for the no-tillage-induced yield loss. This study reveals the role of biochar in main-season rice and ratoon rice cultivation, providing a valuable reference for improved fertilizer utilization and cleaner agricultural production.