Protein phosphorylation can induce signal transduction to change sperm motility patterns during sperm capacitation. However, changes in the phosphorylation of sperm proteins in mice are still incompletely understood. Here, capacitation-related phosphorylation in mouse sperms were firstly investigated by label-free quantitative (LFQ) phosphoproteomics coupled with bioinformatics analysis using ingenuity pathway analysis (IPA) methods such as canonical pathway, upstream regulator, and network analysis. Among 1632 phosphopeptides identified at serine, threonine, and tyrosine residues, 1050 novel phosphosites, corresponding to 402 proteins, were reported. Gene heatmaps for IPA canonical pathways showed a novel role for GSK-3 in GP6 signaling pathways associated with capacitation for 60 min. At the same time, the reduction of the abundant isoform-specific GSK-3α expression was shown by western blot (WB) while the LFQ pY of this isoform slightly decreased and then increased. The combined results from WB and LFQ methods explain the less inhibitory phosphorylation of GSK-3α during capacitation and also support the predicted increases in its activity. In addition, pAKAP4 increased at the Y156 site but decreased at the Y811 site in a capacitated state, even though IPA network analysis and WB analysis for overall pAKAP revealed upregulated trends. The potential roles of GSK-3 and AKAP4 in fertility are discussed.

Background: Concerns have been raised regarding the efficacy and safety resulting from the potential interactions of herbs with Western medications due to the use of both herbs and Western medicine by the general public. Information obtained from the web must be critically evaluated prior to its use in making decisions. Description: This study aimed to construct an herb-drug interaction (HDI) website (https://drug-herb-interaction.netlify.com) with a critically reviewed database. Node.js was used to store the database by running JavaScript. Vue.js is a front-end framework used for web interface development. A total of 135 sets of information related to the interactions of ginseng, ginkgo and dong quai with Western medicine from the literature identified in Medline were collected, followed by critical reviews to prepare nineteen items of information for each HDI monograph. A total of 80 sets of validated HDIs met all criteria and were further assessed at the individual reliability level (likely, possible, and unevaluable) and labeled with the “interaction” item. This query system of the website can be operated in both the Chinese and English languages to obtain all monographs on HDIs in the database, including bilingual interaction data. The database of HDI monographs can be updated by simply uploading a new version of the information Excel file. The designed “smart search” module, in addition to the “single search”, is convenient for requesting multiple searches. Among the “likely” interactions (n = 26), 50% show negative HDIs. Ten of these can increase the effect of the Western drug, and the others (n = 3) imply that the HDI can be beneficial. Conclusions: The current study provides a website platform and 80 sets of validated bilingual HDIs involving ginseng, ginkgo and dong quai in an online database. A search of HDI monographs related to these three herbs can be performed with this bilingual, easy-to-use query website, which is feasible for professionals and the general public. The identified reliability level for each HDI may assist readers’ decisions regarding whether taking Western medications concomitant with one of three herbal medicinal foods is safe or whether caution is required due to potentially serious outcomes.

Inadequate levels of 5-methyltetrahydrofolate (5-MTHF) and the T variant of MTHFR C677T have been suggested to be associated with an increased risk of developing mental illness, whereas the PON1 SNP variant provides a protective role. However, reports validating the methodology for plasma 5-MTHF levels in schizophrenia patients are limited. A sensitive LC–MS/MS system using an amide column and calibration curve was determined by dialyzed human plasma, and applied to schizophrenia patients and healthy controls in Taiwan, and the differences between the subgroups were discussed. This analysis system meets regulation criteria, and the lower limit of quantification for 5-MTHF levels was 4 nM from 200 μL plasma, within 7 min. The mean plasma 5-MTHF levels in schizophrenia patients (n = 34; 11.70 ± 10.37 nM) were lower than those in the healthy controls (n = 42; 22.67 ± 11.12 nM) significantly (p < 0.01). 5-MTHF concentrations were significantly lower in male carriers than in female carriers (18.30 ± 10.37 nM vs. 24.83 ± 11.01 nM, p < 0.05), especially in subjects who were MTHFR CT/PON1 Q allele carriers. In conclusion, this quantitative system, which employed sensitive and simple processing methods, was successfully applied, and identified that schizophrenic patients had significantly lower levels of 5-MTHF. Lower plasma 5-MTHF concentrations were observed in male subjects.

PHBS is a set of behaviors that are practiced based on awareness as an effort to be healthy and actively help the health of the surrounding environment. Clean and Healthy Behavior at an early age is important to maintain personal and environmental health. Early childhood is also susceptible to infectious diseases such as flu and diarrhea if they do not maintain personal and surrounding hygiene. Diarrheal disease is often considered a trivial matter but if it is not addressed it can result in quite serious matters. Therefore, we conducted community services which aims to provide students’ knowledge and understanding about Clean and Healthy Behavior and diarrheal diseases which often attack children at Muhammadiyah 1 Malang Elementary School students. The implementation method is carried out by counseling using power point media and videos. In the initial activity, a question-and-answer session was carried out, then giving educational material, showing educational videos, then evaluating it with a posttest.

Covid-19 pandemic has an impact to the changing educational system, raising the mental health issues, including college students’ psychology. This study explores demographic and knowledge about Covid-19 associated with college student’s depression, anxiety, and stress in online classes during outbreak. We undertook a cross-sectional study by using questionnaires to explore demographic characteristics and knowledge about Covid-19 among college students during the online classes. Mental health was assessed using DASS-42. The logistic regression used to investigate the association between demographic characteristics and knowledge about Covid-19 with mental health among college students. The prevalence of depression among 599 college students was 27.5%, meanwhile the prevalence of anxiety and stress were 44.6% and 21.29%, respectively. Based on the logistic regression, being in second year (OR = .43), having friends who got infected with Covid-19 (OR=.36), and feeling worried for delaying school (OR=.40) was significantly negatively correlated with depression, whereas having difficulties in adaptation of online class (OR=1.78) was significantly positively correlated with depression. The early identification of depression, anxiety, and stress among college students is pivotal and relevant related factors should be considered when developing preventive programs, particularly during online classes.

Stroke is a neurologic deficit resulting from damage to the brain due to disruption of blood flow that occurs suddenly and rapidly. 87% of strokes are ischemic strokes, caused by a lack of oxygen supply to the brain due to embolism, thrombus or atherosclerosis. Blockage of blood flow will disrupt cell function accompanied by necrosis which can then lead to infarction. Antidyslipidemia is given to all ischemic stroke patients as secondary therapy and prevention of recurrent stroke. The use of Antidyslipidemia in ischemic stroke patients improves lipid abnormalities, reduces the occurrence of coronary heart disease, and improves clinical outcomes. This study aim to describe the use pattern of antidyslipidemia in ischemic stroke patients at RSUD R.T. Notopuro Sidoarjo. The type of this research is observational research with retrospective data collection method for the period October 2023 - December 2023 and then analyzed descriptively. The pattern of antidyslipidemia use is a single use pattern, as many as 78 patterns (99%). The most common single use pattern was given atorvastatin (1 x 20 mg) as many as 66 patients (86%). The combination usage pattern given was atorvastatin (1 x 20 mg) po + fenofibrate (1 x 300 mg). The switching use pattern of atorvastatin (1 x 40 mg)po atorvastatin (1 x 20 mg)po was given to 1 patient.