With the development of the petroleum industry, oil pollution has become widespread. It is harmful to the digestive, immune, reproductive, and nervous systems of fishes, wild animals, and humans, causing severe threats to ecological safety and human health. Gordonia has increasingly attracted attention in the treatment of alkane pollution for its outstanding performance against hydrophobic refractory substances. However, the lack of knowledge about alkane uptake and degradation restricts the application of gordonia. In this paper, we studied the strain lys1-3 of Gordonia sihwaniensis isolated from coal chemical wastewater, which showed good alkane degradation performance by lys1-3. It is found that stimulated by an alkane, lys1-3 secreted biosurfactants, which emulsified large alkane particles to smaller particles. By active transport, unmodified alkane was transferred into cells and produced a large amount of acid, which was secreted out of the cells.

Paraffinic n-alkanes (C22–C30), crucial portions of residual oil, are generally considered to be difficult to be biodegraded owing to their general solidity at ambient temperatures and low water solubility, rendering relatively little known about metabolic processes in different methanogenic hydrocarbon-contaminated environments. Here, we established a methanogenic C22–C30 n-alkane-degrading enrichment culture derived from a high-temperature oil reservoir production water. During two-year incubation (736 days), unexpectedly significant methane production was observed. The measured maximum methane yield rate (164.40 μmol L⁻¹ d⁻¹) occurred during the incubation period from day 351 to 513. The nearly complete consumption (> 97%) of paraffinic n-alkanes and the detection of dicarboxylic acids in n-alkane-amended cultures indicated the biotransformation of paraffin to methane under anoxic condition. 16S rRNA gene analysis suggested that the dominant methanogen in n-alkane-degrading cultures shifted from Methanothermobacter on day 322 to Thermoplasmatales on day 736. Bacterial community analysis based on high-throughput sequencing revealed that members of Proteobacteria and Firmicutes exhibiting predominant in control cultures, while microorganisms affiliated with Actinobacteria turned into the most dominant phylum in n-alkane-dependent cultures. Additionally, the relative abundance of mcrA gene based on genomic DNA significantly increased over the incubation time, suggesting an important role of methanogens in these consortia. This work extends our understanding of methanogenic paraffinic n-alkanes conversion and has biotechnological implications for microbial enhanced recovery of residual hydrocarbons and effective bioremediation of hydrocarbon-containing biospheres.

Relative permeability of polymer flooding plays a very important role in oil field development. This paper aimed to measure and calculate the relative permeability curves of polymer flooding more accurately. First, viscosity variation law of polymer in porous media was studied. Rock particles of different diameters and cementing agent were used to make artificial cores and hydrophobically associating polymer solutions were prepared for experiments. Polymer solutions were injected into the cores filled with crude oil and irreducible water. In the process of polymer flooding, produced fluid was collected at different water saturations and locations of the core. Polymer solutions were separated and their viscosities were measured. With the experimental data, the viscosity variation rule of polymer transporting in porous media was explored. The result indicates that the viscosity retention rate of polymer solutions transporting in porous media has power function relationship with the water saturation and the dimensionless distance from the core inlet. Finally, the relative permeability curves of polymer flooding were measured by unsteady state method and the viscosity variation rule was applied to the calculation of the relative permeability curves.

Workflow of molecular dynamics simulations in polymer design and optimization.

The alkali/surfactant/polymer (ASP) flood has long been considered to reduce residual oil saturation significantly after waterflood. This paper provides an experimental investigation of the factors (permeability, pore structure, ASP formula, injection volume, viscosity, and injection volume) that influence the evolution of residual oil after ASP flooding. ASP flood experiments were conducted on the cores drilled in Daqing field, and two-dimensional real-structure micromodels were constructed based on these cores. For the ASP core flood experiments, X-ray computed tomography imaging was used for the visualization of the residual oil evolution. For the ASP micromodel flood experiments, images of the residual oil distribution were obtained using a microscope with a 5× magnification objective. The results showed that as water saturation increased during the flood, the proportion of oil clusters decreased, and the proportion of oil droplets first increased and then decreased. For the cores with smaller pore throats and more complex pore structure, the residual oil became more scattered. In this case, the oil clusters became smaller, and oil droplets became easier to retain. An increased injection rate improved the emulsification, resulting in more residual oil in small pores getting replaced. Increasing the viscosity by increasing the polymer concentration improved the sweep efficiency, mainly because residual oil in large pore throats was displaced, but had a negative impact on emulsification. Increasing the viscosity of the injection fluid was shown to have a negative impact on improving the oil recovery ratio, because the mobilization of residual oil in smaller pores was greatly impacted by emulsification. The effect of increasing injection volume on improving recovery was more pronounced for cores with lower permeability.