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Matrix metalloproteinases (MMPs) play an important role in tooth development and influence caries development and hybrid layer degradation. Literature is scant on the differences in the activity of MMPs between primary and permanent dentine. Accordingly, the aim of the present study was to investigate endogenous gelatinolytic activity in primary and permanent dentine. Separate batches of dentine powder were obtained from intact human primary and permanent molars (n = 6). Each batch was divided in two subgroups: (1) mineralised; and (2) demineralised with 10% H3PO4. After protein extraction, gelatine zymography was performed. Furthermore, in situ zymography was performed on dentine sections of the same groups (n = 3). The slices were polished, covered with fluorescein-conjugated gelatine and evaluated using a confocal microscope. In situ zymography data were analysed using two-way analysis of variance and post hoc Holm–Šidák statistics (α = 0.05). Primary dentine showed poorly defined bands in the zymograms that vaguely corresponded to the pro-form and active form of MMP-2 and the pro-form of MMP-9. In permanent dentine, demineralised powder demonstrated stronger gelatinolytic activity than mineralised powder. In situ zymography identified stronger enzymatic activity in primary etched dentine (p< 0.05). Stronger enzymatic activity recorded in primary dentine may be related to the differences in morphology and composition between primary and permanent dentine.
Tatjana Maravic; Lorenzo Breschi; Federica Paganelli; Giulio Bonetti; Stefano Martina; Gianni Di Giorgio; Maurizio Bossù; Antonella Polimeni; Vittorio Checchi; Luigi Generali; Franklin Tay; Milena Cadenaro; Annalisa Mazzoni. Endogenous Enzymatic Activity of Primary and Permanent Dentine. Materials 2021, 14, 4043 .
AMA StyleTatjana Maravic, Lorenzo Breschi, Federica Paganelli, Giulio Bonetti, Stefano Martina, Gianni Di Giorgio, Maurizio Bossù, Antonella Polimeni, Vittorio Checchi, Luigi Generali, Franklin Tay, Milena Cadenaro, Annalisa Mazzoni. Endogenous Enzymatic Activity of Primary and Permanent Dentine. Materials. 2021; 14 (14):4043.
Chicago/Turabian StyleTatjana Maravic; Lorenzo Breschi; Federica Paganelli; Giulio Bonetti; Stefano Martina; Gianni Di Giorgio; Maurizio Bossù; Antonella Polimeni; Vittorio Checchi; Luigi Generali; Franklin Tay; Milena Cadenaro; Annalisa Mazzoni. 2021. "Endogenous Enzymatic Activity of Primary and Permanent Dentine." Materials 14, no. 14: 4043.
Background: Avulsion and reimplantation of permanent teeth represent a major challenge in terms of treatment and long-term prognosis. The present study reported clinical management of external root resorption of an avulsed and reimplanted maxillary central incisor. Case report: A 9-year-old boy reported an uncomplicated crown fracture and avulsion of tooth 11 and complicated crown fracture of tooth 21 due to trauma. Reimplantation of element 11 was obtained within 30 min post-trauma and 3 days after both elements were diagnosed with necrotic pulp. In addition, tooth 11 showed early external root resorption. Both elements underwent endodontic treatment and root closure with apical plug using calcium-silicate-based cement. At 6-month follow-up root resorption appeared to be arrested. Twenty-four months after trauma the clinical results were stable, although signs and symptoms of ankylosis were observed. Conclusions: An immediate endodontic approach and use of calcium-silicate-based cement seemed to contrast the progression of root resorption of an avulsed and reimplanted central incisor after 24 months of follow-up.
Gianni Di Giorgio; Alessandro Salucci; Gian Sfasciotti; Flavia Iaculli; Maurizio Bossù. External Root Resorption Management of an Avulsed and Reimplanted Central Incisor: A Case Report. Dentistry Journal 2021, 9, 72 .
AMA StyleGianni Di Giorgio, Alessandro Salucci, Gian Sfasciotti, Flavia Iaculli, Maurizio Bossù. External Root Resorption Management of an Avulsed and Reimplanted Central Incisor: A Case Report. Dentistry Journal. 2021; 9 (6):72.
Chicago/Turabian StyleGianni Di Giorgio; Alessandro Salucci; Gian Sfasciotti; Flavia Iaculli; Maurizio Bossù. 2021. "External Root Resorption Management of an Avulsed and Reimplanted Central Incisor: A Case Report." Dentistry Journal 9, no. 6: 72.
Calcium silicate-based cements have reached excellent levels of performance in endodontics, providing predictable and successful results. To better assess the properties of these bioactive materials, the present study aimed to compare the biocompatibility and antibiofilm properties of ProRoot MTA and Biodentine. Human osteogenic sarcoma (Saos-2) cells were cultured on ProRoot MTA and Biodentine samples or in the presence of both cement extracts. Cell viability assay, measurement of reactive oxygen species (ROS), immunofluorescence analysis, as well as morphological evaluations were conducted. Moreover, Streptococcus mutans was used to assess the biofilm forming ability on ProRoot MTA and Biodentine disks. Finally, both cements were applied in vivo to treat immature permanent teeth affected by reversible pulpitis. Results: Cell viability assay demonstrated that Saos-2 cells had a dose- and time-dependent cytotoxicity to both analyzed cements, although cells exposed to ProRoot MTA showed a better cell vitality than those exposed to Biodentine (p< 0.001). Both cements demonstrated ROS production while this was greater in the case of Biodentine than ProRoot MTA (p< 0.001). Immunofluorescence images of the cytoskeleton and focal adhesions showed no differences in Saos-2 cells grown in the presence of ProRoot MTA eluate; whereas in the Biodentine groups, cells showed a morphology and focal adhesions more similar to that of the control sample, as the eluate concentration decreased. Morphological analysis revealed that Saos-2 cells were more flattened and exhibited better spreading when attached to ProRoot MTA disks than to Biodentine ones. The antibiofilm properties showed a time-dependent powerful inhibition of S. mutans superficial colonization and an antibiofilm effect of both cements. Clinically, complete root formation of the treated elements was achieved using the two studied cements, showing stable results over time. ProRoot MTA and Biodentine was demonstrated to be biocompatible and to possess antibiofilm properties. Their clinical application in vital pulp therapy provided successful outcomes after 2 years of follow-up.
Maurizio Bossù; Patrizia Mancini; Erika Bruni; Daniela Uccelletti; Adele Preziosi; Marco Rulli; Michela Relucenti; Orlando Donfrancesco; Flavia Iaculli; Gianni Di Giorgio; Roberto Matassa; Alessandro Salucci; Antonella Polimeni. Biocompatibility and Antibiofilm Properties of Calcium Silicate-Based Cements: An In Vitro Evaluation and Report of Two Clinical Cases. Biology 2021, 10, 470 .
AMA StyleMaurizio Bossù, Patrizia Mancini, Erika Bruni, Daniela Uccelletti, Adele Preziosi, Marco Rulli, Michela Relucenti, Orlando Donfrancesco, Flavia Iaculli, Gianni Di Giorgio, Roberto Matassa, Alessandro Salucci, Antonella Polimeni. Biocompatibility and Antibiofilm Properties of Calcium Silicate-Based Cements: An In Vitro Evaluation and Report of Two Clinical Cases. Biology. 2021; 10 (6):470.
Chicago/Turabian StyleMaurizio Bossù; Patrizia Mancini; Erika Bruni; Daniela Uccelletti; Adele Preziosi; Marco Rulli; Michela Relucenti; Orlando Donfrancesco; Flavia Iaculli; Gianni Di Giorgio; Roberto Matassa; Alessandro Salucci; Antonella Polimeni. 2021. "Biocompatibility and Antibiofilm Properties of Calcium Silicate-Based Cements: An In Vitro Evaluation and Report of Two Clinical Cases." Biology 10, no. 6: 470.
Knowledge of the antibody-mediated immune response to SARS-CoV-2 is crucial to understand virus immunogenicity, establish seroprevalence, and determine whether subjects or recovered patients are at risk for infection/reinfection and would therefore benefit from vaccination. Here, we describe a novel and simple cell-ELISA specifically designed to measure viral spike S1-specific IgG produced in vitro by B cells in peripheral blood mononuclear cell (PBMC) cultures from a cohort of 45 asymptomatic (n = 24) and symptomatic (n = 21) individuals, with age ranging from 8 to 99 years. All subjects underwent ELISA serological screening twice, at the same time as the cell-ELISA (T2) as well as 35–60 days earlier (T1). Cryopreserved PBMCs of healthy donors obtained years before the COVID-19 pandemic were also included in the analysis. The preliminary results presented here show that out of 45 tested subjects, 16 individuals (35.5%) were positive to the cell-ELISA, 11 (24.5%) were concomitantly positive in the serological screening (T1 and/or T2), and only one person was exclusively positive in ELISA (T1) and negative in cell-ELISA, though values were close to the cutoff. Of note, five individuals (11.2%) tested negative in ELISA but positive in cell-ELISA and thus, they appear to have circulating B cells that produce antibodies against SARS-CoV-2, likely at levels that are undetectable in the serum, which challenges the negative results of the serological screening. The relative level of in vitro secreted IgG was measurable in positive subjects, ranging from 7 to 50 ng/well. Accordingly, all anti-SARS-CoV-2 antibody-positive subjects previously reported moderate to severe symptoms attributable to COVID-19, even though the RT-PCR data were rarely available to confirm viral infection. Overall, the described cell-ELISA might be an effective method for detecting subjects who encountered the virus in the past, and thus helpful to improve serological ELISA tests in the case of undetectable/equivocal circulating IgG levels, and a suitable and improved tool to better evaluate SARS-CoV-2-specific humoral immunity in the COVID-19 pandemic.
Gianpaolo Zarletti; Massimo Tiberi; Veronica De Molfetta; Maurizio Bossù; Elisa Toppi; Paola Bossù; Giuseppe Scapigliati. A Cell-Based ELISA to Improve the Serological Analysis of Anti-SARS-CoV-2 IgG. Viruses 2020, 12, 1274 .
AMA StyleGianpaolo Zarletti, Massimo Tiberi, Veronica De Molfetta, Maurizio Bossù, Elisa Toppi, Paola Bossù, Giuseppe Scapigliati. A Cell-Based ELISA to Improve the Serological Analysis of Anti-SARS-CoV-2 IgG. Viruses. 2020; 12 (11):1274.
Chicago/Turabian StyleGianpaolo Zarletti; Massimo Tiberi; Veronica De Molfetta; Maurizio Bossù; Elisa Toppi; Paola Bossù; Giuseppe Scapigliati. 2020. "A Cell-Based ELISA to Improve the Serological Analysis of Anti-SARS-CoV-2 IgG." Viruses 12, no. 11: 1274.
The purpose of this study was to clarify the structural and ultrastructural alterations of the enamel and dentin collagen network in the deciduous teeth of children affected by osteogenesis imperfecta (OI) using field-emission in-lens scanning electron microscopy (FEI-SEM) and transmission electron microscopy (TEM) analyses. Exfoliated primary teeth were collected from children with a diagnosis of OI and from healthy individuals (N = 24). Tooth slices containing both dentin and enamel were fixed, dehydrated and dried, gold sputtered, and observed using FEI-SEM. Additional dentin fragments were decalcified, dehydrated, embedded in resin, cut, and processed for TEM analysis. Under FEI-SEM, the enamel in OI-affected children showed an irregular prism distribution with the enamel hydroxyapatite crystals unpacked. Ultrastructural correlative analysis of the dentin in patients affected by OI showed an altered collagen pattern with a low density. In some areas, teeth in OI patients showed a reduction in the number of dentinal tubules, with odontoblastic process missing in most of the tubules. The presence of altered dentine and enamel organization in OI children was firmly established at an ultrastructural level, but additional biochemical studies are necessary in order to clarify quantitatively and qualitatively the collagenic and non-collagenic proteins in this disorder.
Uros Josic; Tatjana Maravic; Maurizio Bossù; Milena Cadenaro; Allegra Comba; Gaetano Ierardo; Antonella Polimeni; Federica Florenzano; Lorenzo Breschi; Annalisa Mazzoni. Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta. Applied Sciences 2020, 10, 7835 .
AMA StyleUros Josic, Tatjana Maravic, Maurizio Bossù, Milena Cadenaro, Allegra Comba, Gaetano Ierardo, Antonella Polimeni, Federica Florenzano, Lorenzo Breschi, Annalisa Mazzoni. Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta. Applied Sciences. 2020; 10 (21):7835.
Chicago/Turabian StyleUros Josic; Tatjana Maravic; Maurizio Bossù; Milena Cadenaro; Allegra Comba; Gaetano Ierardo; Antonella Polimeni; Federica Florenzano; Lorenzo Breschi; Annalisa Mazzoni. 2020. "Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta." Applied Sciences 10, no. 21: 7835.
Aims: Langerhans Cell Histiocytosis is a rare hematologic disorder usually affecting children and most commonly involving the head and neck region. Primary oro-facial manifestations are rare, and their diagnosis is often challenging as they are numerous and often resemble common pathologies, refractory to conventional medical and/or instrumental treatments. For such reasons, the diagnosis is frequently delayed, as is the following staging and therapy onset. We retrospectively studied 45 pediatric patients affected by Langerhans Cell Histiocytosis with onset in the head and neck, to examine their clinical and radiological features at the early stage. Materials and Methods: The study was a retrospective bi-institutional analysis (Department of Pediatric Dentistry and Pediatric Oncology of “Sapienza” University of Rome, Department of Interdisciplinary Medicine of the University of Bari “Aldo Moro”), which enrolled 45 patients (age range 0–18 year-old) affected by Langerhans Cell Histiocytosis with oro-facial onset. Data regarding clinical appearance, number, site, synchronous or metachronous occurrence, involved tissues/organs, radiographic features and clinical outcomes were collected, listed and overall differentiated by two age ranges (0–10-year-olds and 10–18-year-olds). Results: Patients were 26 males and 19 females, with an average age at the time of diagnosis of 4.8 ± 3.8 years (median = 3.9 years). The most common findings were inflamed, hyperplastic, painful and often ulcerated gingival lesions (22 cases), associated with deciduous tooth mobility and/or dislocation with bone loss in 18 cases, followed by nine single eosinophilic granulomas of the mandible and two of the maxilla. Lesions of the palatal mucosa were observed in six patients; nine patients showed on radiograms the characteristic “floating teeth” appearance in the mandible with synchronous lesions of the maxilla in six. Paresthesia was relatively un-frequent (three cases) and the pathological fracture of the mandible occurred in six. Head/neck lymph nodes involvement was associated with oral lesions in 12 cases and skull lesions in 14. Otitis (media or externa) was detected in four instances, exophthalmia in two, cutaneous rush in nine, contextual presence or subsequent onset of insipidus diabetes in eight. As for therapy, single or multiple small jaw lesions were all surgically removed; chemotherapy with vinblastine alone or associated with corticosteroids was the principal treatment in almost the 80% of cases; more than 50% of patients received corticosteroids, while only three patients received adjunctive radiotherapy. The overall mortality account for less than 9% (four of 45 cases) and recurrence observed in eight patients after therapy. Conclusions: Langerhans Cell Histiocytosis may mimic several oro-facial inflammatory and neoplastic diseases. Considering the potential disabling sequela following head and neck localization of Langerhans Cell Histiocytosis in children, especially at the periodontal tissues with teeth and alveolar bone loss, lesion recognition along with the histological examination of suspicious tissues is mandatory to achieve an early diagnosis and to prevent further organ involvement.
Saverio Capodiferro; Angela Tempesta; Luisa Limongelli; Giuseppe Ingravallo; Eugenio Maiorano; Gian Luca Sfasciotti; Maurizio Bossù; Antonella Polimeni; Gianfranco Favia. Primary Oro-Facial Manifestations of Langerhans Cell Histiocytosis in Pediatric Age: A Bi-Institutional Retrospective Study on 45 Cases. Children 2020, 7, 104 .
AMA StyleSaverio Capodiferro, Angela Tempesta, Luisa Limongelli, Giuseppe Ingravallo, Eugenio Maiorano, Gian Luca Sfasciotti, Maurizio Bossù, Antonella Polimeni, Gianfranco Favia. Primary Oro-Facial Manifestations of Langerhans Cell Histiocytosis in Pediatric Age: A Bi-Institutional Retrospective Study on 45 Cases. Children. 2020; 7 (9):104.
Chicago/Turabian StyleSaverio Capodiferro; Angela Tempesta; Luisa Limongelli; Giuseppe Ingravallo; Eugenio Maiorano; Gian Luca Sfasciotti; Maurizio Bossù; Antonella Polimeni; Gianfranco Favia. 2020. "Primary Oro-Facial Manifestations of Langerhans Cell Histiocytosis in Pediatric Age: A Bi-Institutional Retrospective Study on 45 Cases." Children 7, no. 9: 104.
Lesch-Nyhan syndrome (LNS) is a serious form of hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency, a hereditary purine metabolism disorder. The prevalence reported in the literature is 1/380,000 to 235,000 births. Males are affected and females are heterozygous carriers. LNS patients present a combination of hypotonia, spasticity, and neurological and behavioral disorders. They also show an obsessive-compulsive self-injurious behavior with bites and injuries to the lips, tongue, cheeks and fingers. The literature offers little scientific contribution related to the management of this problem. The authors describe their experience with a 4-year-old LNS patient and present a viable solution to control and avoid bite injuries, namely a specifically modified bite. The patient was treated at the Pediatric Dentistry Department of “Sapienza” University of Rome with a modified bite with the internal surface, in contact with the teeth, realized in 2-mm-thick soft silicone, and the exterior part consisting of a transparent resin shell with front and rear shields to separate lips and cheeks from the dental arches. At a 12-month follow-up visit, compliance was excellent: the child wore the device with regularity and without discomfort, even during soft-food feeding. No intraoral bite injuries were found, with a general improvement of the young patient’s quality of life.
Gaetano Ierardo; Valeria Luzzi; Gian Luca Sfasciotti; Antonella Polimeni; Maurizio Bossù. Lesch-Nyhan Syndrome: Evaluation of a Modified Bite Device to Prevent Bite Injuries. Applied Sciences 2020, 10, 3808 .
AMA StyleGaetano Ierardo, Valeria Luzzi, Gian Luca Sfasciotti, Antonella Polimeni, Maurizio Bossù. Lesch-Nyhan Syndrome: Evaluation of a Modified Bite Device to Prevent Bite Injuries. Applied Sciences. 2020; 10 (11):3808.
Chicago/Turabian StyleGaetano Ierardo; Valeria Luzzi; Gian Luca Sfasciotti; Antonella Polimeni; Maurizio Bossù. 2020. "Lesch-Nyhan Syndrome: Evaluation of a Modified Bite Device to Prevent Bite Injuries." Applied Sciences 10, no. 11: 3808.
Early childhood caries (ECC) is a severe manifestation of carious pathology with rapid and disruptive progression. The ECC microbiota includes a wide variety of bacterial species, among which is an anaerobic newly named species, Scardovia wiggsiae, a previously unidentified Bifidobacterium. Our aim was to provide the first ultrastructural characterization of S. wiggsiae and its biofilm by scanning electron microscopy (SEM) using a protocol that faithfully preserved the biofilm architecture and allowed an investigation at very high magnifications (order of nanometers) and with the appropriate resolution. To accomplish this task, we analyzed Streptococcus mutans’ biofilm by conventional SEM and VP-SEM protocols, in addition, we developed an original procedure, named OsO4-RR-TA-IL, which avoids dehydration, drying and sputter coating. This innovative protocol allowed high-resolution and high-magnification imaging (from 10000× to 35000×) in high-vacuum and high-voltage conditions. After comparing three methods, we chose OsO4-RR-TA-IL to investigate S. wiggsiae. It appeared as a fusiform elongated bacterium, without surface specialization, arranged in clusters and submerged in a rich biofilm matrix, which showed a well-developed micro-canalicular system. Our results provide the basis for the development of innovative strategies to quantify the effects of different treatments, in order to establish the best option to counteract ECC in pediatric patients.
Maurizio Bossù; Laura Selan; Marco Artini; Michela Relucenti; Giuseppe Familiari; Rosanna Papa; Gianluca Vrenna; Patrizia Spigaglia; Fabrizio Barbanti; Alessandro Salucci; Gianni Di Giorgio; Julietta V. Rau; Antonella Polimeni. Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol. Microorganisms 2020, 8, 807 .
AMA StyleMaurizio Bossù, Laura Selan, Marco Artini, Michela Relucenti, Giuseppe Familiari, Rosanna Papa, Gianluca Vrenna, Patrizia Spigaglia, Fabrizio Barbanti, Alessandro Salucci, Gianni Di Giorgio, Julietta V. Rau, Antonella Polimeni. Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol. Microorganisms. 2020; 8 (6):807.
Chicago/Turabian StyleMaurizio Bossù; Laura Selan; Marco Artini; Michela Relucenti; Giuseppe Familiari; Rosanna Papa; Gianluca Vrenna; Patrizia Spigaglia; Fabrizio Barbanti; Alessandro Salucci; Gianni Di Giorgio; Julietta V. Rau; Antonella Polimeni. 2020. "Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol." Microorganisms 8, no. 6: 807.
Background: The objective of this study is to evaluate the application of National guidelines for prevention and clinical management of traumatic dental injuries (NGPCMTDI) in developmental age published by the Italian Ministry of Health. Methods: In the present retrospective and multicenter study, 246 patients who underwent dental injury were selected to assess the management of the traumatic event compiled with the protocol provided by the National guidelines. Each health worker involved completed a form related to the dental injury in order to standardize the collected data. Two reference centers have been identified for data collection. Analyses for comparisons between groups were performed using the X2 test for categorical variables or by Fisher exact test as appropriate. Statistical significance was assumed at p < 0.05. Results: Evaluating the distribution by age we concluded that: 27.24% of the enrolled patients were aged 1–5 years, 51.63% 6–10 years, and 19.92% 11–17 years. The dental injuries occurred in 10.16% of the situations at home, 50.81% at school, 28.86% during recreation, and 9.35% at the gym. The deciduous dentition is involved in 34.96% of the traumas while the permanent dentition is involved in 69.51%. Conclusion: From the present study it emerged that the National guidelines are not uniformly applied.
Maurizio Bossù; Francesco Covello; Gianni Di Giorgio; Stefania Zampogna; Valentina Talarico; Salvatore De Filippo; Antonella Polimeni; Stefano Di Carlo. Evaluation of the Application of Italian National Guidelines for Prevention and Management of Dental Injuries in Developmental Age. International Journal of Environmental Research and Public Health 2020, 17, 2875 .
AMA StyleMaurizio Bossù, Francesco Covello, Gianni Di Giorgio, Stefania Zampogna, Valentina Talarico, Salvatore De Filippo, Antonella Polimeni, Stefano Di Carlo. Evaluation of the Application of Italian National Guidelines for Prevention and Management of Dental Injuries in Developmental Age. International Journal of Environmental Research and Public Health. 2020; 17 (8):2875.
Chicago/Turabian StyleMaurizio Bossù; Francesco Covello; Gianni Di Giorgio; Stefania Zampogna; Valentina Talarico; Salvatore De Filippo; Antonella Polimeni; Stefano Di Carlo. 2020. "Evaluation of the Application of Italian National Guidelines for Prevention and Management of Dental Injuries in Developmental Age." International Journal of Environmental Research and Public Health 17, no. 8: 2875.
Today, biomaterial research on biomimetic mineralization strategies represents a new challenge in the prevention and cure of enamel mineral loss on delicate deciduous teeth. Distinctive assumptions about the origin, the growth, and the functionalization on the biomimetic materials have been recently proposed by scientific research studies in evaluating the different clinical aspects of treating the deciduous tooth. Therefore, appropriate morpho-chemical observations on delivering specific biomaterials to enamel teeth is the most important factor for controlling biomineralization processes. Detailed morpho-chemical investigations of the treated enamel layer using three commercial toothpastes (Biorepair, F1400, and F500) were performed through variable pressure scanning electron microscopy (VP-SEM) and energy dispersive X-ray spectroscopy (EDS) on deciduous teeth in their native state. A new microscopy methodology allowed us to determine the behaviors of silicate, phosphate, and calcium contents from the early stage, as commercially available toothpastes, to the final stage of delivered diffusion, occurring within the enamel layer together with their penetration depth properties. The reported results represent a valuable background towards full comprehension of the role of organic–inorganic biomaterials for developing a controlled biomimetic toothpaste in biofluid media.
Maurizio Bossù; Roberto Matassa; Michela Relucenti; Flavia Iaculli; Alessandro Salucci; Gianni Di Giorgio; Giuseppe Familiari; Antonella Polimeni; Stefano Di Carlo. Morpho-Chemical Observations of Human Deciduous Teeth Enamel in Response to Biomimetic Toothpastes Treatment. Materials 2020, 13, 1803 .
AMA StyleMaurizio Bossù, Roberto Matassa, Michela Relucenti, Flavia Iaculli, Alessandro Salucci, Gianni Di Giorgio, Giuseppe Familiari, Antonella Polimeni, Stefano Di Carlo. Morpho-Chemical Observations of Human Deciduous Teeth Enamel in Response to Biomimetic Toothpastes Treatment. Materials. 2020; 13 (8):1803.
Chicago/Turabian StyleMaurizio Bossù; Roberto Matassa; Michela Relucenti; Flavia Iaculli; Alessandro Salucci; Gianni Di Giorgio; Giuseppe Familiari; Antonella Polimeni; Stefano Di Carlo. 2020. "Morpho-Chemical Observations of Human Deciduous Teeth Enamel in Response to Biomimetic Toothpastes Treatment." Materials 13, no. 8: 1803.
During the period of health emergency linked to the current COVID-19 pandemic, the management of children's oral health presents specific problems related to the infectious spread of the disease. These problems must be faced on the one hand by acting on the oral health prevention methods, and on the other by implementing specific protocols relating both to the conditions of oral pathologies that normally do not represent an emergency, and to those clinical situations that fall into the category of pediatric dental emergencies. In this perspective, in addition to defining rigorous and highly effective infection control protocols in the dental settings, it is of fundamental importance to work on remote communication and education aimed at maintaining the oral health of the children. This article, after an analysis of the risk factors from COVID-19 associated with pediatric dental treatment, presents a series of considerations on potential oral prevention strategies and on the management of emergency and non-emergency dental procedures in a context of disease transmission control, proposing new approaches and models of treatment based also on remote interaction techniques which will then retain their usefulness even at the end of the current emergency period.
Valeria Luzzi; Gaetano Ierardo; Maurizio Bossù; Antonella Polimeni. COVID-19: Pediatric Oral Health During and After the Pandemics. 2020, 1 .
AMA StyleValeria Luzzi, Gaetano Ierardo, Maurizio Bossù, Antonella Polimeni. COVID-19: Pediatric Oral Health During and After the Pandemics. . 2020; ():1.
Chicago/Turabian StyleValeria Luzzi; Gaetano Ierardo; Maurizio Bossù; Antonella Polimeni. 2020. "COVID-19: Pediatric Oral Health During and After the Pandemics." , no. : 1.
Children with autism spectrum disorder (ASD) have significantly higher prevalence and caries severity compared to the average population. Knowledge about the oral health indices of children with this mental disorder is key to designing efficient plans of intervention. This paper reports the results of a study on the oral health status of children with ASD in central Italy. This is the first study of this type in Italy. The sample consists of 229 autistic children aged between 5 and 14 years, attending the Unit of Special Needs Policlinico Umberto I in Rome. Each patient received an intraoral examination to investigate decayed, missing, and filled teeth as well as periodontal status. Information on demographic attributes, dietary habits, medical history, and child’s cooperativeness at the first visit was also recorded. Of the participants, 79.26% presented signs of gingivitis and about 90% of them had plaque. Caries prevalence was 66.38%. The average of the total number of decayed, missing, and filled teeth in the permanent and primary dentition was 2.91. Among the factors considered, only dietary habits and the periodontal indices showed statistically significant association with caries prevalence and caries severity. Despite the selection bias, that prevents us to interpret the results presented as epidemiological evidence, our study suggests that children with ASD in central Italy represent a population at risk.
Maurizio Bossù; Mario Trottini; Denise Corridore; Gianni Di Giorgio; Gian Luca Sfasciotti; Gaspare Palaia; Livia Ottolenghi; Antonella Polimeni; Stefano Di Carlo. Oral Health Status of Children with Autism in Central Italy. Applied Sciences 2020, 10, 2247 .
AMA StyleMaurizio Bossù, Mario Trottini, Denise Corridore, Gianni Di Giorgio, Gian Luca Sfasciotti, Gaspare Palaia, Livia Ottolenghi, Antonella Polimeni, Stefano Di Carlo. Oral Health Status of Children with Autism in Central Italy. Applied Sciences. 2020; 10 (7):2247.
Chicago/Turabian StyleMaurizio Bossù; Mario Trottini; Denise Corridore; Gianni Di Giorgio; Gian Luca Sfasciotti; Gaspare Palaia; Livia Ottolenghi; Antonella Polimeni; Stefano Di Carlo. 2020. "Oral Health Status of Children with Autism in Central Italy." Applied Sciences 10, no. 7: 2247.
Background: Pulpotomy of primary teeth provides favorable clinical results over time; however, to date, there is still not a consensus on an ideal pulp dressing material. Therefore, the aim of the present systematic review was to compare pulpotomy agents to establish a preferred material to use. Methods: After raising a PICO question, the PRISMA guideline was adopted to carry out an electronic search through the MEDLINE database to identify comparative studies on several pulp dressing agents, published up to October 2019. Results: The search resulted in 4274 records; after exclusion, a total of 41 papers were included in the present review. Mineral trioxide aggregate (MTA), Biodentine and ferric sulphate yielded good clinical results over time and might be safely used in the pulpotomies of primary molars. Among agents, MTA seemed to be the material of choice. On the contrary, calcium hydroxide showed the worst clinical performance. Although clinically successful, formocreosol should be replaced by other materials, due to its potential cytotoxicity and carcinogenicity. Conclusion: MTA seemed to be the gold standard material in the pulpotomy of primary teeth. Promising results were also provided by calcium silicate-based cements. Further randomized clinical trials (RCTs) with adequate sample sizes and long follow-ups are encouraged to support these outcomes.
Maurizio Bossù; Flavia Iaculli; Gianni Di Giorgio; Alessandro Salucci; Antonella Polimeni; Stefano Di Carlo. Different Pulp Dressing Materials for the Pulpotomy of Primary Teeth: A Systematic Review of the Literature. Journal of Clinical Medicine 2020, 9, 838 .
AMA StyleMaurizio Bossù, Flavia Iaculli, Gianni Di Giorgio, Alessandro Salucci, Antonella Polimeni, Stefano Di Carlo. Different Pulp Dressing Materials for the Pulpotomy of Primary Teeth: A Systematic Review of the Literature. Journal of Clinical Medicine. 2020; 9 (3):838.
Chicago/Turabian StyleMaurizio Bossù; Flavia Iaculli; Gianni Di Giorgio; Alessandro Salucci; Antonella Polimeni; Stefano Di Carlo. 2020. "Different Pulp Dressing Materials for the Pulpotomy of Primary Teeth: A Systematic Review of the Literature." Journal of Clinical Medicine 9, no. 3: 838.
Calcium hydroxide apexification and Mineral Trioxide Aggregate (MTA) apexification are classical treatments for necrotic immature permanent teeth. The first tend to fail for lack of compliance given the high number of sessions needed; the second has technical difficulties such as material manipulation and overfilling. With both techniques, the root development is interrupted leaving the tooth with a fragile root structure, a poor crown-to-root ratio, periodontal breakdown, and high risk of fracture, compromising long-term prognosis of the tooth. New scientific literature has described a procedure that allows complete root development of these specific teeth. This regenerative endodontic procedure (REP) proposes the use of a combination of antimicrobials and irrigants, no canal walls instrumentation, induced apical bleeding to form a blood clot and a tight seal into the root canal to promote healing. MTA is the most used material to perform this seal, but updated guidelines advise the use of other bioactive endodontic cements that incorporate calcium and silicate in their compositions. They share most of their characteristics with MTA but claim to have fewer drawbacks with regards to manipulation and aesthetics. The purpose of the present article is to review pertinent literature and to describe the clinical procedures protocol with its variations, and their clinical application.
Simone Staffoli; Gianluca Plotino; Barbara G. Nunez Torrijos; Nicola M. Grande; Maurizio Bossù; Gianluca Gambarini; Antonella Polimeni. Regenerative Endodontic Procedures Using Contemporary Endodontic Materials. Materials 2019, 12, 908 .
AMA StyleSimone Staffoli, Gianluca Plotino, Barbara G. Nunez Torrijos, Nicola M. Grande, Maurizio Bossù, Gianluca Gambarini, Antonella Polimeni. Regenerative Endodontic Procedures Using Contemporary Endodontic Materials. Materials. 2019; 12 (6):908.
Chicago/Turabian StyleSimone Staffoli; Gianluca Plotino; Barbara G. Nunez Torrijos; Nicola M. Grande; Maurizio Bossù; Gianluca Gambarini; Antonella Polimeni. 2019. "Regenerative Endodontic Procedures Using Contemporary Endodontic Materials." Materials 12, no. 6: 908.
The propagation of antibiotic resistance increases the chances of major infections for patients during hospitalization and the spread of health related diseases. Therefore finding new and effective solutions to prevent the proliferation of pathogenic microorganisms is critical, in order to protect hospital environment, such as the surfaces of biomedical devices. Modern nanotechnology has proven to be an effective countermeasure to tackle the threat of infections. On this note, recent scientific breakthroughs have demonstrated that antimicrobial nanomaterials are effective in preventing pathogens from developing resistance. Despite the ability to destroy a great deal of bacteria and control the outbreak of infections, nanomaterials present many other advantages. Moreover, it is unlikely for nanomaterials to develop resistance due to their multiple and simultaneous bactericidal mechanisms. In recent years, science has explored more complex antimicrobial coatings and nanomaterials based on graphene have shown great potential in antibacterial treatment. The purpose of this article is to deepen the discussion on the threat of infections related to surface disinfection and to assess the state of the art and potential solutions, with specific focus on disinfection procedures using nanomaterials.
Matteo Saccucci; Erika Bruni; Daniela Uccelletti; Agnese Bregnocchi; Maria Sabrina Sarto; Maurizio Bossù; Gabriele Di Carlo; Antonella Polimeni. Surface Disinfections: Present and Future. Journal of Nanomaterials 2018, 2018, 1 -9.
AMA StyleMatteo Saccucci, Erika Bruni, Daniela Uccelletti, Agnese Bregnocchi, Maria Sabrina Sarto, Maurizio Bossù, Gabriele Di Carlo, Antonella Polimeni. Surface Disinfections: Present and Future. Journal of Nanomaterials. 2018; 2018 ():1-9.
Chicago/Turabian StyleMatteo Saccucci; Erika Bruni; Daniela Uccelletti; Agnese Bregnocchi; Maria Sabrina Sarto; Maurizio Bossù; Gabriele Di Carlo; Antonella Polimeni. 2018. "Surface Disinfections: Present and Future." Journal of Nanomaterials 2018, no. : 1-9.
Background Coeliac disease is a chronic small intestinal immune‐mediated enteropathy precipitated by exposure to dietary gluten, and possible relationships between coeliac disease and dental pathogenic conditions during childhood have been poorly investigated. Aim The dental pulp plays a pivotal role in the immune defence against possible entry of pathogens from teeth, and the aim of this work was to investigate quantitative transcription levels of selected genes (IL‐9, IL‐11, IL‐15, IL‐18, IL‐21, IL‐27, MICA, IFN‐γ) coding for pro‐inflammatory immune innate activities in the pulp of primary teeth from healthy children and children with coeliac disease. Design The pulp from primary teeth of 10 healthy children and 10 children with coeliac disease was used to extract RNA and prepare cDNA for quantitative PCR transcription analysis employing commercial nucleotide probes for selected genes. Results In children with coeliac disease, the genes coding for pro‐inflammatory cytokines IFN‐γ, IL‐11, IL‐18, and IL‐21 were significantly overexpressed, suggesting the possible importance of these cytokines in the relationships between coeliac disease and dental disorders. Conclusion For the first time, we reported in dental pulp of children possible relationships between coeliac disease and modulation in transcription of cytokine‐dependent inflammatory activities.
Maurizio Bossù; Monica Montuori; Daniela Casani; Gianni Di Giorgio; Andrea Pacifici; Barbara Ladniak; Antonella Polimeni. Altered transcription of inflammation-related genes in dental pulp of coeliac children. International Journal of Paediatric Dentistry 2015, 26, 351 -356.
AMA StyleMaurizio Bossù, Monica Montuori, Daniela Casani, Gianni Di Giorgio, Andrea Pacifici, Barbara Ladniak, Antonella Polimeni. Altered transcription of inflammation-related genes in dental pulp of coeliac children. International Journal of Paediatric Dentistry. 2015; 26 (5):351-356.
Chicago/Turabian StyleMaurizio Bossù; Monica Montuori; Daniela Casani; Gianni Di Giorgio; Andrea Pacifici; Barbara Ladniak; Antonella Polimeni. 2015. "Altered transcription of inflammation-related genes in dental pulp of coeliac children." International Journal of Paediatric Dentistry 26, no. 5: 351-356.
The aim of this study was to analyze the effects of orthopedic therapy with rapid maxillary expansion (RME) in growing patients affected by osteogenesis imperfecta and treated with bisphosphonates. Three boys with osteogenesis imperfecta (mean age, 10.6 years) were treated with RME. They all had treatment with quarterly intravenous infusions of bisphosphonates. They were in either the early or the late mixed dentition and had indications for RME. The expansion screw was activated twice daily until correction of the transverse relationships was achieved. The retention period with the expander in place was 6 months. In 2 Class III patients, RME was associated with the use of a facemask. In all patients, occlusal radiographs were taken at the end of active RME therapy to assess the opening of the midpalatal suture and 1 year after the end of active expansion therapy to evaluate the reossification and reorganization of the midpalatal suture. In all patients, the opening of the midpalatal suture and the healing with reorganization of the midpalatal suture were documented with the occlusal radiographs. No complications were found after a 1-year follow-up. In growing patients affected by osteogenesis imperfecta and treated with bisphosphonates, it is possible to perform RME with a standard protocol with no complications after a 1-year follow-up.
Gaetano Ierardo; Francesca Calcagnile; Valeria Luzzi; Barbara Ladniak; Maurizio Bossu; Mauro Celli; Anna Zambrano; Lorenzo Franchi; Antonella Polimeni. Osteogenesis imperfecta and rapid maxillary expansion: Report of 3 patients. American Journal of Orthodontics and Dentofacial Orthopedics 2015, 148, 130 -137.
AMA StyleGaetano Ierardo, Francesca Calcagnile, Valeria Luzzi, Barbara Ladniak, Maurizio Bossu, Mauro Celli, Anna Zambrano, Lorenzo Franchi, Antonella Polimeni. Osteogenesis imperfecta and rapid maxillary expansion: Report of 3 patients. American Journal of Orthodontics and Dentofacial Orthopedics. 2015; 148 (1):130-137.
Chicago/Turabian StyleGaetano Ierardo; Francesca Calcagnile; Valeria Luzzi; Barbara Ladniak; Maurizio Bossu; Mauro Celli; Anna Zambrano; Lorenzo Franchi; Antonella Polimeni. 2015. "Osteogenesis imperfecta and rapid maxillary expansion: Report of 3 patients." American Journal of Orthodontics and Dentofacial Orthopedics 148, no. 1: 130-137.
Aim The chemical-physical properties of novel and long-standing calcium silicate cements versus conventional pulp capping calcium hydroxide biomaterials were compared. Methods Calcium hydroxide–based (Calxyl, Dycal, Life, Lime-Lite) and calcium silicate–based (ProRoot MTA, MTA Angelus, MTA Plus, Biodentine, Tech Biosealer capping, TheraCal) biomaterials were examined. Calcium and hydroxyl ion release, water sorption, interconnected open pores, apparent porosity, solubility and apatite-forming ability in simulated body fluid were evaluated. Results All calcium silicate materials released more calcium. Tech Biosealer capping, MTA Plus gel and Biodentine showed the highest values of calcium release, while Lime-Lite the lowest. All the materials showed alkalizing activity except for Life and Lime-Lite. Calcium silicate materials showed high porosity values: Tech Biosealer capping, MTA Plus gel and MTA Angelus showed the highest values of porosity, water sorption and solubility, while TheraCal the lowest. The solubility of water-containing materials was higher and correlated with the liquid-to-powder ratio. Calcium phosphate (CaP) deposits were noted on materials surfaces after short aging times. Scant deposits were detected on Lime-Lite. A CaP coating composed of spherulites was detected on all calcium silicate materials and Dycal after 28 days. The thickness, continuity and Ca/P ratio differed markedly among the materials. MTA Plus showed the thickest coating, ProRoot MTA showed large spherulitic deposits, while TheraCal presented very small dense spherulites. Conclusions calcium silicate-based cements are biointeractive (ion-releasing) bioactive (apatite-forming) functional biomaterials. The high rate of calcium release and the fast formation of apatite may well explain the role of calcium silicate biomaterials as scaffold to induce new dentin bridge formation and clinical healing.
Maria Giovanna Gandolfi; Francesco Siboni; Tatiana Botero; Maurizio Bossù; Francesco Riccitiello; Carlo Prati. Calcium Silicate and Calcium Hydroxide Materials for Pulp Capping: Biointeractivity, Porosity, Solubility and Bioactivity of Current Formulations. Journal of Applied Biomaterials & Functional Materials 2015, 13, 43 -60.
AMA StyleMaria Giovanna Gandolfi, Francesco Siboni, Tatiana Botero, Maurizio Bossù, Francesco Riccitiello, Carlo Prati. Calcium Silicate and Calcium Hydroxide Materials for Pulp Capping: Biointeractivity, Porosity, Solubility and Bioactivity of Current Formulations. Journal of Applied Biomaterials & Functional Materials. 2015; 13 (1):43-60.
Chicago/Turabian StyleMaria Giovanna Gandolfi; Francesco Siboni; Tatiana Botero; Maurizio Bossù; Francesco Riccitiello; Carlo Prati. 2015. "Calcium Silicate and Calcium Hydroxide Materials for Pulp Capping: Biointeractivity, Porosity, Solubility and Bioactivity of Current Formulations." Journal of Applied Biomaterials & Functional Materials 13, no. 1: 43-60.
Aim: Calcium silicate-based materials are hydraulic self-setting materials with physico-chemical properties suitable for endodontic surgery and good biological/clinical outcomes. The study aim was to evaluate the bio-properties (biointeractivity and apatite-forming ability) and selected physical properties (porosity, water sorption, solubility, and setting time) of Biodentine, a tricalcium silicate material for endodontics and restorative dentistry, compared to that of ProRoot MTA (Mineral Trioxide Aggregate) as gold standard material. Methods: Biodentine and ProRoot MTA pastes were prepared and analyzed for calcium release and alkalinizing activity (3 h–28 days), setting time, water sorption, porosity, solubility, surface microstructure and composition, and apatite-forming ability in simulated body fluid. Results: Biodentine showed higher calcium release, alkalinizing activity, and solubility but higher open and apparent porosity, water sorption, and a markedly shorter setting time. Calcium phosphate (CaP) deposits were noted on material surfaces after short ageing times. A CaP coating composed of spherulites was detected after 28 days. The thickness, continuity, and Ca/P ratio of the coating differed markedly between the materials. Biodentine showed a coating composed by denser but smaller spherulites, while ProRoot MTA showed large but less dense aggregates of spherulitic deposits. Conclusions: Biodentine showed a pronounced ability to release calcium and extended alkalinizing activity interlinked with its noticeable porosity, water sorption, and solubility: open porosities provide a broad wet biointeractive surface for the release of the calcium and hydroxyl ions involved in the formation of a CaP mineral. Biodentine is a biointeractive tricalcium silicate material with interesting chemical-physical properties and represents a fast-setting alternative to the conventional calcium silicate MTA-like cements.
Maria Giovanna Gandolfi; Francesco Siboni; Antonella Polimeni; Maurizio Bossù; Francesco Riccitiello; Sandro Rengo; Carlo Prati. In Vitro Screening of the Apatite-Forming Ability, Biointeractivity and Physical Properties of a Tricalcium Silicate Material for Endodontics and Restorative Dentistry. Dentistry Journal 2013, 1, 41 -60.
AMA StyleMaria Giovanna Gandolfi, Francesco Siboni, Antonella Polimeni, Maurizio Bossù, Francesco Riccitiello, Sandro Rengo, Carlo Prati. In Vitro Screening of the Apatite-Forming Ability, Biointeractivity and Physical Properties of a Tricalcium Silicate Material for Endodontics and Restorative Dentistry. Dentistry Journal. 2013; 1 (4):41-60.
Chicago/Turabian StyleMaria Giovanna Gandolfi; Francesco Siboni; Antonella Polimeni; Maurizio Bossù; Francesco Riccitiello; Sandro Rengo; Carlo Prati. 2013. "In Vitro Screening of the Apatite-Forming Ability, Biointeractivity and Physical Properties of a Tricalcium Silicate Material for Endodontics and Restorative Dentistry." Dentistry Journal 1, no. 4: 41-60.
Recurrent aphthous stomatitis is a common disorder of the oral cavity, affecting mainly young people. It is characterized by small ulcers which can be very painful and generally heal spontaneously within 7–14 days. There is currently no therapy that can provide rapid healing. This study evaluated the efficacy and rapidity of response of a lozenge containing Lactobacillus brevis CD2. 30 patients were randomized to take 4 lozenges a day of active product or placebo for 7 days. Signs and symptoms as well as laboratory parameters in the saliva were assessed at the start of the study and after 7 days of treatment. The study demonstrated the efficacy and the rapidity of response of the Lactobacillus brevis CD2 lozenges in resolving the clinical signs and symptoms of aphthous stomatitis, with a significantly rapid improvement of pain. This is the first study confirming the efficacy of a probiotic product in this pathology.
Vito Trinchieri; Stefano Di Carlo; Maurizio Bossu'; Antonella Polimeni. Use of Lozenges Containing Lactobacillus brevis CD2 in Recurrent Aphthous Stomatitis: A Double-Blind Placebo-Controlled Trial. Ulcers 2011, 2011, 1 -6.
AMA StyleVito Trinchieri, Stefano Di Carlo, Maurizio Bossu', Antonella Polimeni. Use of Lozenges Containing Lactobacillus brevis CD2 in Recurrent Aphthous Stomatitis: A Double-Blind Placebo-Controlled Trial. Ulcers. 2011; 2011 ():1-6.
Chicago/Turabian StyleVito Trinchieri; Stefano Di Carlo; Maurizio Bossu'; Antonella Polimeni. 2011. "Use of Lozenges Containing Lactobacillus brevis CD2 in Recurrent Aphthous Stomatitis: A Double-Blind Placebo-Controlled Trial." Ulcers 2011, no. : 1-6.