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Maria Campos is Professor at Faculty of Pharmacy, University of Coimbra (Portugal) where she did her Ph.D. The postdoctoral studies were in 2000 at Industrial Research, Ltd (New Zealand). She gives lectures in Pharmacognosy, Medicinal Plants, Natural Source Products, Phytotherapy. Supervise MD, PhD and Postdoc students mainly in Drug Discovery. She wrote 4 books, 17 chapters,180 papers scientific journals and technic-professional publications. She gave more than 170 conferences. Belong to editorial board of 7 scientific journals. As International Expert she works in the WHO Global network/ IRCH, in the ISO- TC034-SC19-WG3 normalization and was member of the Isoflavones ESCO Comission/EFSA.
Soy and its bioactive compounds, isoflavones, have become a hot issue in the last 20 years. Increased breeding of the plant has created a strong market in many countries. Nevertheless, the cross-consumption of foreign products is not safe for certain ethnic groups and requires risk assessment evaluation because of ethnic differences among populations that induce, for certain compounds, different end points in humans. For example, in Asians and white people, the intake of these compounds can have different bioactive effects. Chemically, isoflavones are isoflavonoids, a subgroup of polyphenolic compounds that have affinity for estrogen receptors and can act as endocrine disruptors in white people. Isoflavones from soy can also be found as constituents of astragalus roots, adzuki beans, chaste, green peas, chickpeas, lupins, kudzu, and red clover, among other plants. An extract named okara also contains isoflavones in variable concentrations and is a by-product of the production of soy preparations, such as tofu and soybean beverages. The main isoflavones isolated from soy are the malonyl glycosides of genistein, daidzein, and glycitein; these aglycones appear only in very low levels. All of these compounds undergo enterohepatic metabolism and are detoxicated by cytochrome P450 (CYP) 1A2 and 3A4, which imply an important potential to induce drug–herb interactions with concomitant intake. Recent research on bioactivity/toxicity of isoflavones has provided a perspective for potential health improvement, but sometimes these results are hard to compare in different ethnic groups. Correct evaluation of each extract in animal and clinical trials is the key to understanding the end points in order to assess safety. This chapter discusses, in detail, the future challenges for isoflavone-derived products, especially in the Western market.
Maria Graça Campos. Soy Isoflavones. Handbook of Dietary Phytochemicals 2021, 205 -242.
AMA StyleMaria Graça Campos. Soy Isoflavones. Handbook of Dietary Phytochemicals. 2021; ():205-242.
Chicago/Turabian StyleMaria Graça Campos. 2021. "Soy Isoflavones." Handbook of Dietary Phytochemicals , no. : 205-242.
This study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using standard validated methods. Bacterial identification was performed for each sample and each colony type using Vitek® 2 Compact 15 system and PCR amplification using 16S rDNA bacterial universal primers (27F, 1492R), species being confirm by sequences analysis. In five raw honey samples, we have identified probiotic bacteria, such as: Bacillus mycoides, Bacillus thuringiensis, Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus velezensis. Generally, all honey samples meet the standard values for chemical composition. However, one sample having 7.44% sucrose was found to have also probiotics bacteria from the genus Bacillus because sucrose is a substrate for probiotics development. In conclusion, the Romanian raw honey can be a potential reservoir of probiotics, which confer a health benefit for consumers.
Claudia Pașca; Liviu Mărghitaș; Ioana Matei; Victorița Bonta; Rodica Mărgăoan; Florina Copaciu; Otilia Bobiș; Maria Campos; Daniel Dezmirean. Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation. Applied Sciences 2021, 11, 5816 .
AMA StyleClaudia Pașca, Liviu Mărghitaș, Ioana Matei, Victorița Bonta, Rodica Mărgăoan, Florina Copaciu, Otilia Bobiș, Maria Campos, Daniel Dezmirean. Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation. Applied Sciences. 2021; 11 (13):5816.
Chicago/Turabian StyleClaudia Pașca; Liviu Mărghitaș; Ioana Matei; Victorița Bonta; Rodica Mărgăoan; Florina Copaciu; Otilia Bobiș; Maria Campos; Daniel Dezmirean. 2021. "Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation." Applied Sciences 11, no. 13: 5816.
Bee pollen is commonly reputed as a rich source of nutrients, both for bees and humans. Its composition is well balanced and can be taken as a stand-alone food or as supplement, including for the elderly owing its low caloric value. However, storage conditions frequently lead to product degradation, namely due to the high moisture content that enable the proliferation of molds and bacteria. Herein, an infrared (IR)-based technology is proposed as a mean to determine moisture content, setting also a new scalable approach for the development of a drying technology to be used for bee pollen processing, which can be carried out in a short time, without impacting the phenolic and flavonoid content and associated bioactive effects. Proof-of-concept was attained with an IR moisture analyzer, bee pollen samples from Eucalyptus globulus Labill and Salix atrocinerea Brot. being selected as models. Impact of the IR radiation towards the phenolic and flavonoid profiles was screened by HPLC/DAD profiling and radical scavenging ability by the DPPH assay. The IR-based approach shows good reproducibility while simultaneously reducing drying time and energy consumption, thus implying a low environmental impact and being suitable for industrial scale-up once no degradation has been found to occur during the radiation process.
Maria Campos; Christian Frigerio; Otilia Bobiş; Adriana Urcan; Nelson Gomes. Infrared Irradiation Drying Impact on Bee Pollen: Case Study on the Phenolic Composition of Eucalyptus globulus Labill and Salix atrocinerea Brot. Pollens. Processes 2021, 9, 890 .
AMA StyleMaria Campos, Christian Frigerio, Otilia Bobiş, Adriana Urcan, Nelson Gomes. Infrared Irradiation Drying Impact on Bee Pollen: Case Study on the Phenolic Composition of Eucalyptus globulus Labill and Salix atrocinerea Brot. Pollens. Processes. 2021; 9 (5):890.
Chicago/Turabian StyleMaria Campos; Christian Frigerio; Otilia Bobiş; Adriana Urcan; Nelson Gomes. 2021. "Infrared Irradiation Drying Impact on Bee Pollen: Case Study on the Phenolic Composition of Eucalyptus globulus Labill and Salix atrocinerea Brot. Pollens." Processes 9, no. 5: 890.
Valerianaceae, the sub-family of Caprifoliaceae, contains more than 300 species of annual and perennial herbs, worldwide distributed. Several species are used for their biological properties while some are used as food. Species from the genus Valeriana have been used for their antispasmodic, relaxing, and sedative properties, which have been mainly attributed to the presence of valepotriates, borneol derivatives, and isovalerenic acid. Among this genus, the most common and employed species is Valeriana officinalis. Although valerian has been traditionally used as a mild sedative, research results are still controversial regarding the role of the different active compounds, the herbal preparations, and the dosage used. The present review is designed to summarize and critically describe the current knowledge on the different plant species belonging to Valerianaceae, their phytochemicals, their uses in the treatment of different diseases with particular emphasis on the effects on the central nervous system. The available information on this sub-family was collected from scientific databases up until year 2020. The following electronic databases were used: PubMed, Scopus, Sci Finder, Web of Science, Science Direct, NCBI, and Google Scholar. The search terms used for this review included Valerianaceae, Valeriana, Centranthus, Fedia, Patrinia, Nardostachys, Plectritis, and Valerianella, phytochemical composition, in vivo studies, Central Nervous System, neuroprotective, antidepressant, antinociceptive, anxiolytic, anxiety, preclinical and clinical studies.
Gitishree Das; Han-Seung Shin; Rosa Tundis; Sandra Gonçalves; Ourlad Tantengco; Maria Campos; Rosaria Acquaviva; Giuseppe Malfa; Anabela Romano; Joyce Robles; Mariel Clores; Jayanta-Kumar Patra. Plant Species of Sub-Family Valerianaceae—A Review on Its Effect on the Central Nervous System. Plants 2021, 10, 846 .
AMA StyleGitishree Das, Han-Seung Shin, Rosa Tundis, Sandra Gonçalves, Ourlad Tantengco, Maria Campos, Rosaria Acquaviva, Giuseppe Malfa, Anabela Romano, Joyce Robles, Mariel Clores, Jayanta-Kumar Patra. Plant Species of Sub-Family Valerianaceae—A Review on Its Effect on the Central Nervous System. Plants. 2021; 10 (5):846.
Chicago/Turabian StyleGitishree Das; Han-Seung Shin; Rosa Tundis; Sandra Gonçalves; Ourlad Tantengco; Maria Campos; Rosaria Acquaviva; Giuseppe Malfa; Anabela Romano; Joyce Robles; Mariel Clores; Jayanta-Kumar Patra. 2021. "Plant Species of Sub-Family Valerianaceae—A Review on Its Effect on the Central Nervous System." Plants 10, no. 5: 846.
Beebread (BB) is a fermented bee product made from plant pollen, honey and bee saliva. The nutritive and biological compounds of BB vary greatly depending on the diversity of flora and the season of pollen collection by bees. In order to evaluate this valuable bee product potential, thirteen BB samples were analyzed on the nutritive proprieties and biologically active compounds, correlated with their botanical origin. The obtained results shows that BB comes with a high content of proteins with values ranging between 18.96 and 23.11 g/100 g, lipids ranging between 4.89 and 14.74 g/100 g, and free sugars, fructose being present in the highest amount (17.27 g/100 g). Analyzed samples contain all the essential amino acids, methionine being the most abundant, 86.93–12.38 mg/100 g, mono- and polyunsaturated fatty acids, ω-6/ω-3 ratio varying between 0.02 – 1.07. BB samples have also high amount of natural antioxidants, from the class of polyphenols and flavonol glycoside derivative. These findings suggest that BB could be used as a source of potential value-added nutrients and bioactive compounds and contributes to the chemical knowledge of this natural product.
Adriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Otilia Bobiș; Victorița Bonta; Francisc Vasile Dulf; Rodica Mărgăoan; Mihaiela Cornea-Cipcigan; Maria Graça Campos. Botanical origin approach for a better understanding of chemical and nutritional composition of beebread as an important value-added food supplement. LWT - Food Science and Technology 2021, 142, 111068 .
AMA StyleAdriana Cristina Urcan, Adriana Dalila Criste, Daniel Severus Dezmirean, Otilia Bobiș, Victorița Bonta, Francisc Vasile Dulf, Rodica Mărgăoan, Mihaiela Cornea-Cipcigan, Maria Graça Campos. Botanical origin approach for a better understanding of chemical and nutritional composition of beebread as an important value-added food supplement. LWT - Food Science and Technology. 2021; 142 ():111068.
Chicago/Turabian StyleAdriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Otilia Bobiș; Victorița Bonta; Francisc Vasile Dulf; Rodica Mărgăoan; Mihaiela Cornea-Cipcigan; Maria Graça Campos. 2021. "Botanical origin approach for a better understanding of chemical and nutritional composition of beebread as an important value-added food supplement." LWT - Food Science and Technology 142, no. : 111068.
Soy and its bioactive compounds, isoflavones, have become a hot issue in the last 20 years. Increased breeding of the plant has created a strong market in many countries. Nevertheless, the cross-consumption of foreign products is not safe for certain ethnic groups and requires risk assessment evaluation because of ethnic differences among populations that induce, for certain compounds, different end points in humans. For example, in Asians and white people, the intake of these compounds can have different bioactive effects. Chemically, isoflavones are isoflavonoids, a subgroup of polyphenolic compounds that have affinity for estrogen receptors and can act as endocrine disruptors in white people. Isoflavones from soy can also be found as constituents of astragalus roots, adzuki beans, chaste, green peas, chickpeas, lupins, kudzu, and red clover, among other plants. An extract named okara also contains isoflavones in variable concentrations and is a by-product of the production of soy preparations, such as tofu and soybean beverages. The main isoflavones isolated from soy are the malonyl glycosides of genistein, daidzein, and glycitein; these aglycones appear only in very low levels. All of these compounds undergo enterohepatic metabolism and are detoxicated by cytochrome P450 (CYP) 1A2 and 3A4, which imply an important potential to induce drug–herb interactions with concomitant intake. Recent research on bioactivity/toxicity of isoflavones has provided a perspective for potential health improvement, but sometimes these results are hard to compare in different ethnic groups. Correct evaluation of each extract in animal and clinical trials is the key to understanding the end points in order to assess safety. This chapter discusses, in detail, the future challenges for isoflavone-derived products, especially in the Western market.
Maria Graça Campos. Soy Isoflavones. Handbook of Dietary Phytochemicals 2020, 1 -38.
AMA StyleMaria Graça Campos. Soy Isoflavones. Handbook of Dietary Phytochemicals. 2020; ():1-38.
Chicago/Turabian StyleMaria Graça Campos. 2020. "Soy Isoflavones." Handbook of Dietary Phytochemicals , no. : 1-38.
Royal jelly (RJ) is a yellowish-white and acidic secretion of hypopharyngeal and mandibular glands of nurse bees used to feed young worker larvae during the first three days and the entire life of queen bees. RJ is one of the most appreciated and valued natural product which has been mainly used in traditional medicines, health foods, and cosmetics for a long time in different parts of the world. It is also the most studied bee product, aimed at unravelling its bioactivities, such as antimicrobial, antioxidant, anti-aging, immunomodulatory, and general tonic action against laboratory animals, microbial organisms, farm animals, and clinical trials. It is commonly used to supplement various diseases, including cancer, diabetes, cardiovascular, and Alzheimer’s disease. Here, we highlight the recent research advances on the main bioactive compounds of RJ, such as proteins, peptides, fatty acids, and phenolics, for a comprehensive understanding of the biochemistry, biological, and pharmaceutical responses to human health promotion and life benefits. This is potentially important to gain novel insight into the biological and pharmaceutical properties of RJ.
Saboor Ahmad; Maria Graça Campos; Filippo Fratini; Solomon Zewdu Altaye; Jianke Li. New Insights into the Biological and Pharmaceutical Properties of Royal Jelly. International Journal of Molecular Sciences 2020, 21, 382 .
AMA StyleSaboor Ahmad, Maria Graça Campos, Filippo Fratini, Solomon Zewdu Altaye, Jianke Li. New Insights into the Biological and Pharmaceutical Properties of Royal Jelly. International Journal of Molecular Sciences. 2020; 21 (2):382.
Chicago/Turabian StyleSaboor Ahmad; Maria Graça Campos; Filippo Fratini; Solomon Zewdu Altaye; Jianke Li. 2020. "New Insights into the Biological and Pharmaceutical Properties of Royal Jelly." International Journal of Molecular Sciences 21, no. 2: 382.
Mihaiela Cornea-Cipcigan; Doru Pamfil; Cristian Sisea; Cornelia Gavriș; Maria Da Graça Ribeiro Campos; Rodica Mărgăoan. A REVIEW ON CYCLAMEN SPECIES: TRANSCRIPTION FACTORS VS. PHARMACOLOGICAL EFFECTS. Acta Poloniae Pharmaceutica - Drug Research 2019, 76, 919 -938.
AMA StyleMihaiela Cornea-Cipcigan, Doru Pamfil, Cristian Sisea, Cornelia Gavriș, Maria Da Graça Ribeiro Campos, Rodica Mărgăoan. A REVIEW ON CYCLAMEN SPECIES: TRANSCRIPTION FACTORS VS. PHARMACOLOGICAL EFFECTS. Acta Poloniae Pharmaceutica - Drug Research. 2019; 76 (6):919-938.
Chicago/Turabian StyleMihaiela Cornea-Cipcigan; Doru Pamfil; Cristian Sisea; Cornelia Gavriș; Maria Da Graça Ribeiro Campos; Rodica Mărgăoan. 2019. "A REVIEW ON CYCLAMEN SPECIES: TRANSCRIPTION FACTORS VS. PHARMACOLOGICAL EFFECTS." Acta Poloniae Pharmaceutica - Drug Research 76, no. 6: 919-938.
ResumoEste trabalho pretende fazer uma breve análise da história da toxicologia e dos usos que as plantas tóxicas e os seus venenos foram tendo ao longo da história da humanidade, nomeadamente na Antiguidade. As possibilidades de utilização das plantas são praticamente ilimitadas. Assim, pode afirmar-se que a sua importância para o ser humano é inquestionável. O Homem recorre a elas desde a Pré-História, não só pelo seu valor nutricional e terapêutico, mas atribuindo-lhes, também, significados religiosos, estéticos, poéticos e morais. As práticas médicas ancestrais usavam as plantas com fins curativos, podendo considerar-se a fitoterapia como a forma de tratamento mais antiga. Esta prática manteve-se até ao final do séc. XIX/início do séc. XX, quando se isolaram as primeiras substâncias químicas bioactivas das plantas medicinais de origem (ex.: aspirina, digitalina, morfina).À semelhança das suas propriedades medicinais, também o conhecimento e uso dos venenos das plantas remonta à Antiguidade, tendo sido utilizados com diversos propósitos (ex.: caça, pesca, guerra, suicídio, homicídio).O conhecimento da história da toxicologia tem interesse no âmbito de várias disciplinas das áreas das ciências naturais e da saúde, como a medicina, a farmácia, a biologia, a bioquímica, as ciências forenses, entre outras.Palavras-chave: Envenenamentos; Plantas tóxicas; Venenos.AbstractThis work intends to make a brief analysis of the history of toxicology and the uses that toxic plants and their poisons have had throughout the history of mankind, namely in the Antiquity.The possibilities for plant use are practically unlimited. Thus, we can affirm that its importance to the human being is unquestionable. Man uses them since the Prehistory, not only for their nutritional and therapeutic value, but also attributing them religious, aesthetic, poetic and moral meanings. Ancient medical practices used plants for healing purposes, and phytotherapy may be considered as the oldest form of treatment. This practice continued until the end of the 19th century/early 20th century, when the first bioactive chemicals were isolated from the medicinal plants of origin (e.g. aspirin, digitalin, morphine). Like its medicinal properties, the knowledge and use of plant poisons can be traced back to Antiquity, and have been used for various purposes (e.g. hunting, fishing, war, suicide, homicide). Knowledge of the history of toxicology is of interest in various disciplines in the fields of natural sciences and health, such as medicine, pharmacy, biology, biochemistry, forensic sciences, among others.Keywords: Poisoning; Toxic plants; Poisons.
Natacha Catarina Perpétuo; Maria Da Graça Campos; Paulo Renato Trincão; António Pereira Coutinho. Breve história da toxicologia vegetal: alguns usos das plantas tóxicas ao longo do tempo. História da Ciência e Ensino: construindo interfaces 2019, 20, 248 -264.
AMA StyleNatacha Catarina Perpétuo, Maria Da Graça Campos, Paulo Renato Trincão, António Pereira Coutinho. Breve história da toxicologia vegetal: alguns usos das plantas tóxicas ao longo do tempo. História da Ciência e Ensino: construindo interfaces. 2019; 20 ():248-264.
Chicago/Turabian StyleNatacha Catarina Perpétuo; Maria Da Graça Campos; Paulo Renato Trincão; António Pereira Coutinho. 2019. "Breve história da toxicologia vegetal: alguns usos das plantas tóxicas ao longo do tempo." História da Ciência e Ensino: construindo interfaces 20, no. : 248-264.
Bee bread is the main source of proteins necessary for the development of hypopharyngeal glands in nurse bees. However, the seasonal chemical composition and its effects on honey bee physiology are poorly understood. Thus, this study evaluated how the season and botanical origin of pollen influence the content of crude protein, ash, and total flavonoids, as well as the profile of flavonoids, in Africanized Apis mellifera bee bread. The influence of these factors on the number and area of hypopharyngeal gland acini of nurse bees raised with similar numbers of brood frames over the four seasons of the year were also evaluated. The botanical families found in bee bread and the total flavonoid content and their chemical profile varied markedly with the seasons of the year, with the highest total flavonoid content found in the summer. The percentage of crude protein and ash in bee bread, however, did not change with the seasons. The number and area of acini in the hypopharyngeal gland significantly decreased during autumn and winter. Based on our results, flavonoids rather than crude protein may play a role in hypopharyngeal gland development in honey bees. Further studies to test artificial diets supplemented with flavonoids can improve beekeeping strategies and contribute to colony maintenance in periods of food shortage.
Thaís De Souza Bovi; André Caeiro; Sérgio Alexandre Alcantara Dos Santos; Rodrigo Zaluski; Alex Junji Shinohara; Giuseppina Pace Pereira Lima; Maria Da Graça Ribeiro Campos; Luis Antonio Justulin Junior; Ricardo De Oliveira Orsi. Seasonal variation of flavonoid content in bee bread: Potential impact on hypopharyngeal gland development in Apis mellifera honey bees. Journal of Apicultural Research 2019, 59, 170 -177.
AMA StyleThaís De Souza Bovi, André Caeiro, Sérgio Alexandre Alcantara Dos Santos, Rodrigo Zaluski, Alex Junji Shinohara, Giuseppina Pace Pereira Lima, Maria Da Graça Ribeiro Campos, Luis Antonio Justulin Junior, Ricardo De Oliveira Orsi. Seasonal variation of flavonoid content in bee bread: Potential impact on hypopharyngeal gland development in Apis mellifera honey bees. Journal of Apicultural Research. 2019; 59 (2):170-177.
Chicago/Turabian StyleThaís De Souza Bovi; André Caeiro; Sérgio Alexandre Alcantara Dos Santos; Rodrigo Zaluski; Alex Junji Shinohara; Giuseppina Pace Pereira Lima; Maria Da Graça Ribeiro Campos; Luis Antonio Justulin Junior; Ricardo De Oliveira Orsi. 2019. "Seasonal variation of flavonoid content in bee bread: Potential impact on hypopharyngeal gland development in Apis mellifera honey bees." Journal of Apicultural Research 59, no. 2: 170-177.
Bee products were historically used as a therapheutic approach and in food consumption, while more recent data include important details that could validate them as food supplements due to their bioproperties, which support their future use as medicines. In this review data, data collected from bee pollen (BP) and bee bread (BB) essays will be discussed and detailed for their nutritional and health protective properties as functional foods. Dietary antioxidants intake derived from BP and BB have been associated with the prevention and clinical treatment of multiple diseases. The beneficial effects of BP and BB on health result from the presence of multiple polyphenols which possess anti-inflammatory properties, phytosterols and fatty acids, which play anticancerogenic roles, as well as polysaccharides, which stimulate immunological activity. From the main bioactivity studies with BP and BB, in vitro studies and animal experiments, the stimulation of apoptosis and the inhibition of cell proliferation in multiple cell lines could be one of the major therapeutic adjuvant effects to be explored in reducing tumor growth. Tables summarizing the main data available in this field and information about other bio-effects of BP and BB, which support the conclusions, are provided. Additionally, a discussion about the research gaps will be presented to help further experiments that complete the tree main World Health Organization (WHO) Directives of Efficiency, Safety and Quality Control for these products.
Rodica Mărgăoan; Mirela Stranț; Alina Varadi; Erkan Topal; Banu Yücel; Mihaiela Cornea-Cipcigan; Maria G. Campos; Dan C. Vodnar. Bee Collected Pollen and Bee Bread: Bioactive Constituents and Health Benefits. Antioxidants 2019, 8, 568 .
AMA StyleRodica Mărgăoan, Mirela Stranț, Alina Varadi, Erkan Topal, Banu Yücel, Mihaiela Cornea-Cipcigan, Maria G. Campos, Dan C. Vodnar. Bee Collected Pollen and Bee Bread: Bioactive Constituents and Health Benefits. Antioxidants. 2019; 8 (12):568.
Chicago/Turabian StyleRodica Mărgăoan; Mirela Stranț; Alina Varadi; Erkan Topal; Banu Yücel; Mihaiela Cornea-Cipcigan; Maria G. Campos; Dan C. Vodnar. 2019. "Bee Collected Pollen and Bee Bread: Bioactive Constituents and Health Benefits." Antioxidants 8, no. 12: 568.
The effect of Rhodiola sachalinensis Boriss extract irradiated with 50 kGy gamma rays (HKC) on benign prostatic hyperplasia (BPH) was investigated. Seven-week-old male SD rats received a subcutaneous injection of 20 mg/kg of testosterone propionate (TP) to induce BPH. Then, the testosterone only group received testosterone, the testosterone + finasteride group received testosterone and finasteride (5 mg/kg), the testosterone + HKC group received testosterone and HKC extract (500 mg/kg). Prostate weight and the dihydrotestosterone (DHT) levels in serum or prostate tissue were determined. The mRNA expressions of 5-alpha reductase (AR) in prostate tissue were also measured. Compared to the control group, prostate weight was significantly improved in the TP group and decreased in the HKC and finasteride-treated groups. Furthermore, the mRNA expression of 5-AR in the prostate was significantly reduced in the HKC and finasteride-treated groups. Similarly, the expression levels of α-smooth muscle actin (α-SMA) and cytokeratin, which are associated with prostatic enlargement in the HKC and finasteride groups, were much lower than in the TP group. HKC treatment showed similar efficacy to finasteride treatment on rats with testosterone-induced BPH. HKC may be explored as a potential new drug for BPH treatment.
Qi Xin; Mi-Jin Kwon; Ju-Woon Lee; Kwan-Soo Kim; Hao Chen; Maria G. Campos; Rosa Tundis; Cheng-Bi Cui; Young Ho Cho; Hui Cao. Gamma Irradiated Rhodiola sachalinensis Extract Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Downregulating 5-Alpha Reductase and Restoring Testosterone in Rats. Molecules 2019, 24, 3981 .
AMA StyleQi Xin, Mi-Jin Kwon, Ju-Woon Lee, Kwan-Soo Kim, Hao Chen, Maria G. Campos, Rosa Tundis, Cheng-Bi Cui, Young Ho Cho, Hui Cao. Gamma Irradiated Rhodiola sachalinensis Extract Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Downregulating 5-Alpha Reductase and Restoring Testosterone in Rats. Molecules. 2019; 24 (21):3981.
Chicago/Turabian StyleQi Xin; Mi-Jin Kwon; Ju-Woon Lee; Kwan-Soo Kim; Hao Chen; Maria G. Campos; Rosa Tundis; Cheng-Bi Cui; Young Ho Cho; Hui Cao. 2019. "Gamma Irradiated Rhodiola sachalinensis Extract Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Downregulating 5-Alpha Reductase and Restoring Testosterone in Rats." Molecules 24, no. 21: 3981.
This study was carried out to evaluate the in vitro potential antioxidant activity, total phenolics, flavones, and flavonol content of hydro-ethanolic extracts of pollen from fourteen plants belonging to ten families. The identification of phenolic and flavonoid compounds was accomplished using HPLC-DAD analysis. The results of the total phenolic contents varied from 9.20 ± 0.12 mg GAE (Gallic acid equivalent)/g in the pollen sample of Malva sylvestris, to 71.20 ± 0.72 mg GAE (Gallic acid equivalent)/g in the pollen of Mentha spicata, while flavones and flavonols content ranged from 1.27 ± 0.07 mg QE (Quercetin equivalent)/g in the pollen of Scolymus hispanicus to 15.44 ± 1.14 mg QE (Quercetin equivalent)/g in the pollen samples collected from Anacyclus radiatus. The total antioxidant capacity assessed using the molybdate assay ranged between 17.84 ± 1.03 mg AAE (ascorbic acid equivalent)/g in Malva sylvestris to 99.54 ± 0.90 mg AAE (ascorbic acid equivalent)/g in Capparis spinosa pollen. Concerning antioxidant ability, Mentha spicata and Mentha pulegium had the best antioxidant ability. The HPLC-DAD analysis showed a wide variety of flavonols and flavones, mainly glycosides of apigenin, luteolin, kaempferol, quercetin, and isorhamnetin. The phenolic acids are represented by derivatives of caffeic acid, p-hydroxybenzoic acid, and vanillic acid. In Malva sylvestris and Scolymus hispanicus some compounds found could be alkaloids, however, in this work the confirmation of such chemical group was not carried out but it will be included in further studies.
Meryem Bakour; Maria Da Graça Campos; Hamada Imtara; Badiâa Lyoussi. Antioxidant content and identification of phenolic/flavonoid compounds in the pollen of fourteen plants using HPLC-DAD. Journal of Apicultural Research 2019, 59, 35 -41.
AMA StyleMeryem Bakour, Maria Da Graça Campos, Hamada Imtara, Badiâa Lyoussi. Antioxidant content and identification of phenolic/flavonoid compounds in the pollen of fourteen plants using HPLC-DAD. Journal of Apicultural Research. 2019; 59 (1):35-41.
Chicago/Turabian StyleMeryem Bakour; Maria Da Graça Campos; Hamada Imtara; Badiâa Lyoussi. 2019. "Antioxidant content and identification of phenolic/flavonoid compounds in the pollen of fourteen plants using HPLC-DAD." Journal of Apicultural Research 59, no. 1: 35-41.
Erika Fernanda De Matos Vieira; Flávia Cristina Araújo Lucas; Maria Da Graça Ribeiro Campos; Carolina Mesquita Germano. Mururé (Brosimum acutifolium Huber) in the treatment of syphilis in colonial Amazonia: historical data to the actual contribution to treatment. Acta Botanica Brasilica 2019, 33, 183 -190.
AMA StyleErika Fernanda De Matos Vieira, Flávia Cristina Araújo Lucas, Maria Da Graça Ribeiro Campos, Carolina Mesquita Germano. Mururé (Brosimum acutifolium Huber) in the treatment of syphilis in colonial Amazonia: historical data to the actual contribution to treatment. Acta Botanica Brasilica. 2019; 33 (2):183-190.
Chicago/Turabian StyleErika Fernanda De Matos Vieira; Flávia Cristina Araújo Lucas; Maria Da Graça Ribeiro Campos; Carolina Mesquita Germano. 2019. "Mururé (Brosimum acutifolium Huber) in the treatment of syphilis in colonial Amazonia: historical data to the actual contribution to treatment." Acta Botanica Brasilica 33, no. 2: 183-190.
Ligustrum lucidum Aiton and its berries have been used in Chinese traditional medicine for around two thousand years. In the present study, L. lucidium berries harvested in two regions of Portugal were studied. Haemolytic activity and inhibition of oxidative haemolysis as well as the enzyme inhibitory activities (α-amylase enzyme and acetylcholinesterase) were assessed. Results suggest that the different biological activities varied according to the region where samples were collected. Results demonstrated that the sample obtained from region R1 was the most efficient extract for all parameters evaluated, presenting the lowest values of IC50, 10.67 ± 0.46 μg/mL for the inhibition of erythrocyte oxidative haemolysis, 58.28 ± 3.77 μg/mL for the α-amylase enzyme and 67.67 ± 2.10 μg/mL for the acetylcholinesterase inhibition. L. Lucidum berries may be an interesting source of compounds for use in the development of the therapeutic armamentarium for diseases where enzymatic disruption is believed to play a role.
Vanessa B. Paula; Teresa Delgado; Maria Graça Campos; Ofélia Anjos; Leticia M. Estevinho. Enzyme Inhibitory Potential of Ligustrum lucidum Aiton Berries. Molecules 2019, 24, 1283 .
AMA StyleVanessa B. Paula, Teresa Delgado, Maria Graça Campos, Ofélia Anjos, Leticia M. Estevinho. Enzyme Inhibitory Potential of Ligustrum lucidum Aiton Berries. Molecules. 2019; 24 (7):1283.
Chicago/Turabian StyleVanessa B. Paula; Teresa Delgado; Maria Graça Campos; Ofélia Anjos; Leticia M. Estevinho. 2019. "Enzyme Inhibitory Potential of Ligustrum lucidum Aiton Berries." Molecules 24, no. 7: 1283.
Bee Bread samples from Romania and India were analysed by microscopy and High Performance Liquid Chromatography with Diode Array Detection (HPLC/DAD) and compared with pollen from the correspondent taxa. The quantification of sugars, fructose/glucose ratio, total phenolics and flavonoids was also carried out. From the results was possible to identify Brassica and Eucalyptus samples that present similar HPLC/DAD profiles with the respective ultraviolet (UV) identification of the main compounds as Kaempferol-3-O-glycosides and Hydrocinnamic acid derivatives. The Fructose/Glucose (F/G) ratio and the total amounts of phenolics and flavonoids was in line with the prevalence of the specie identified. These coincident fingerprints gave the identification of the samples, as was previously proposed for bee pollens. This paper relates for the first time the achievement on the taxon carried out previously only for bee pollens. It was reported for the first time that this phenolic profile remains unchanged in the case of floral pollen (hand collected), bee pollen and bee bread. Despite the biochemical transformation that occurs during the fermentation of bee bread, it seems that these phenolic compounds are not affected and remain unchanged. Also, variables such as soil and climate do not seem to influence these compounds for the kind of samples under study.
Adriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Rodica Mărgăoan; André Caeiro; Maria Graça Campos. Similarity of Data from Bee Bread with the Same Taxa Collected in India and Romania. Molecules 2018, 23, 2491 .
AMA StyleAdriana Cristina Urcan, Adriana Dalila Criste, Daniel Severus Dezmirean, Rodica Mărgăoan, André Caeiro, Maria Graça Campos. Similarity of Data from Bee Bread with the Same Taxa Collected in India and Romania. Molecules. 2018; 23 (10):2491.
Chicago/Turabian StyleAdriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Rodica Mărgăoan; André Caeiro; Maria Graça Campos. 2018. "Similarity of Data from Bee Bread with the Same Taxa Collected in India and Romania." Molecules 23, no. 10: 2491.
Background: Tamoxifen is an important drug in chemotherapy being part of various protocols in cancer treatment, but is not universally effective even when used before surgery or in preventing recurrence. Pharmacogenetic variability in drug metabolism is one possible mechanism of treatment failure. We hypothesize that Drug-Herb Interactions (DHI) contribute in disease outcomes, nevertheless the functional single nucleotide polymorphisms in drug metabolizing enzymes that activate cytochrome enzymes. Methods: We performed a retrospective study in the last 9 years of clinical cases enrolled in follow up of Observatory of Drug-Herb Interactions, University of Coimbra - Portugal (www.oipm.uc.pt), and the data collected will be discussed in this paper. Data obtained from PubMed and from PubChem Compounds with preference given to the data obtained during the last 10 years, was also obtained. The search terms were varied depending of the Clinical situation. Results: From our experience, in order to avoid the major predictable DHI more observational trials should be carried out for therapeutic protocols associated to tamoxifen. The most consumed, fruits and vegetables, medicinal plants and other natural products associated to the intake of tamoxifen were, for example, Orange and Better juice, Aloe, Geranium, Saint John Wort leaves and flowers, roots as Astragalus, Curcuma, Ginger, Ginseng, Rehmanniae and Valeriana, mushrooms as Coriolus, Maitake, Shiitake and Reishi. Conclusions: Based on the major compounds involved in those products will be predictable how to advice patients doing this kind of treatment and follow a possible therapy failure, or even a toxic event. Our group did a flyer with the main possible interactions selected for DHI with Tamoxifen. This it will be given to patients doing therapy with tamoxifen, helping them to be a aware of the situation and involving all in the resolution for a better outcome (it will be available in our website as other materials for download already posted there).
Campos Maria Graça; Mariana Cupido; Raquel Tavares; Raquel Consul. Clinical Outcomes from Tamoxifen Drug-herb Interactions. International Journal of Clinical Pharmacology & Pharmacotherapy 2018, 3, 1 .
AMA StyleCampos Maria Graça, Mariana Cupido, Raquel Tavares, Raquel Consul. Clinical Outcomes from Tamoxifen Drug-herb Interactions. International Journal of Clinical Pharmacology & Pharmacotherapy. 2018; 3 (2):1.
Chicago/Turabian StyleCampos Maria Graça; Mariana Cupido; Raquel Tavares; Raquel Consul. 2018. "Clinical Outcomes from Tamoxifen Drug-herb Interactions." International Journal of Clinical Pharmacology & Pharmacotherapy 3, no. 2: 1.
A 67-year-old Caucasian male with lung cancer was presented to the Emergency Department with asthenia, anorexia, jaundice and choluria. The patient's lung cancer was being treated medically by a combination of paclitaxel/carboplatin with bi-monthly frequency. The patient was also self-medicating with several natural products, including Chlorella (520 mg/day), Silybum marianum (total of 13.5 mg silymarin/day), zinc sulphate (5.5 mg), selenium (50 μg) and 15 g/day of Curcuma longa. In first chemotherapy cycle no toxicity was observed even he was taking other medications as budesonide and sitagliptin. The toxic events started only after the introduction of the dietary products. Chlorella had contamination with cyanobacteria (Oscillatoriales) and 1.08 μg of cyanotoxin Microcystin-LR (MC-LR) per gram of biomass was found. Patient was consuming ca 0.01 μg MC-LR/kg/day. This case report describes the first known case of paclitaxel toxicity probably related to pharmacokinetic interaction with Turmeric and a contaminated Chlorella supplement resulting in an acute toxic hepatitis and the impact on oncologic patient health.
Maria Luísa Costa; José A. Rodrigues; Joana Azevedo; Vitor Vasconcelos; Eduardo Eiras; Maria Graça Campos. Hepatotoxicity induced by paclitaxel interaction with turmeric in association with a microcystin from a contaminated dietary supplement. Toxicon 2018, 150, 207 -211.
AMA StyleMaria Luísa Costa, José A. Rodrigues, Joana Azevedo, Vitor Vasconcelos, Eduardo Eiras, Maria Graça Campos. Hepatotoxicity induced by paclitaxel interaction with turmeric in association with a microcystin from a contaminated dietary supplement. Toxicon. 2018; 150 ():207-211.
Chicago/Turabian StyleMaria Luísa Costa; José A. Rodrigues; Joana Azevedo; Vitor Vasconcelos; Eduardo Eiras; Maria Graça Campos. 2018. "Hepatotoxicity induced by paclitaxel interaction with turmeric in association with a microcystin from a contaminated dietary supplement." Toxicon 150, no. : 207-211.
The present work is focused on the characterisation of the polyphenolic content of an Oxalis pes-caprae L. leaf extract and on the evaluation of its bioactivity with particular interest on its vascular activity and antioxidant potential. The polyphenolic content was characterised by HPLC-DAD and LC-MS/MS. The vascular activity was evaluated according to the influence on the serotonergic and adrenergic systems of the human internal mammary artery (HIMA). Antioxidant and neuroprotective studies were also conducted. Several luteolin and apigenin derivatives were identified as main constituents of the extract, which did not present any contractile effect nor had any effect on the serotonergic system of HIMA. However, it showed antagonistic effect on the adrenergic system, inhibiting the contraction to noradrenaline (reduction of 58.44% of maximum contraction). The extract showed antioxidant activity and standardised luteolin and apigenin derivatives showed neuroprotective potential, particularly homoorientin.
Marisa Costa Gaspar; Diogo Fonseca; Manuel J. Antunes; Christian Frigerio; Nelson Gomes; Marta Vieira; Armanda E. Santos; Maria Teresa Cruz; Maria D. Cotrim; Maria G. Campos. Polyphenolic characterisation and bioactivity of an Oxalis pes-caprae L. leaf extract. Natural Product Research 2017, 32, 732 -738.
AMA StyleMarisa Costa Gaspar, Diogo Fonseca, Manuel J. Antunes, Christian Frigerio, Nelson Gomes, Marta Vieira, Armanda E. Santos, Maria Teresa Cruz, Maria D. Cotrim, Maria G. Campos. Polyphenolic characterisation and bioactivity of an Oxalis pes-caprae L. leaf extract. Natural Product Research. 2017; 32 (6):732-738.
Chicago/Turabian StyleMarisa Costa Gaspar; Diogo Fonseca; Manuel J. Antunes; Christian Frigerio; Nelson Gomes; Marta Vieira; Armanda E. Santos; Maria Teresa Cruz; Maria D. Cotrim; Maria G. Campos. 2017. "Polyphenolic characterisation and bioactivity of an Oxalis pes-caprae L. leaf extract." Natural Product Research 32, no. 6: 732-738.
Good habits of breastfeeding have been associated to many long-term health benefits. Nowadays, gains are found in the health of children and mothers that practice exclusive breastfeeding for the first six months of life. The search of new potent stimulants of milk production is important to promote lactation, mainly in cases when the breesfeeding is a difficult task. This report presents a case of a woman that accidentally realized an abundant production of milk and high breast tension a few hours after consuming chamomile infusion. Although usual consume of chamomile during pregnancy and lactation are documented to several purposes the galactogogue effect was never reported. In this case report we document for the first time the influence of chamomile infusion in a lactating woman increasing lactogenesis. This article also highlights the need of more research in this field to assure the safety of the intake, by women, of herbal product without risks for them or for the newborns.
Fernando V. Silva; Francisca Dias; Gustavo Costa; Maria Da Graça Campos. Chamomile reveals to be a potent galactogogue: the unexpected effect. The Journal of Maternal-Fetal & Neonatal Medicine 2017, 31, 116 -118.
AMA StyleFernando V. Silva, Francisca Dias, Gustavo Costa, Maria Da Graça Campos. Chamomile reveals to be a potent galactogogue: the unexpected effect. The Journal of Maternal-Fetal & Neonatal Medicine. 2017; 31 (1):116-118.
Chicago/Turabian StyleFernando V. Silva; Francisca Dias; Gustavo Costa; Maria Da Graça Campos. 2017. "Chamomile reveals to be a potent galactogogue: the unexpected effect." The Journal of Maternal-Fetal & Neonatal Medicine 31, no. 1: 116-118.