Pathogenic bacteria were analyzed using the most probable (MPN) method, and histamine level was analyzed using spectrofluorometer. The best liquid smoke concentration was determined using a sensory hedonic test including smell, taste and texture. In addition, water content and pH were also analysed. Concentration of liquid smoke varied between 0, 0.4, 0.8, 1.0 and 1.2%. The best concentration of liquid smoke was 1%. Fresh fillet dipped in 1% liquid smoke showed significantly decreased total plate count (TPC), from 4.1 x 10³ CFU/g in fresh fillet to 7.4 x 10² CFU/g in fillet dipped for 20 minutes. Analysis of pathogenic bacteria showed a positive result in fresh fillet, and a negative result in fillets dipped in 1% liquid smoke, after 2 days of incubation at 30°C. Analysis of anti-pathogenic bacteria showed the positive results in fresh fillet, and negative result fillets dipped in 1% liquid smoke. Further analysis of histamine content also supports our finding, in which the level of histamine goes down from 19.55 to 18.56 when dipped in 1% liquid smoke solution for 20 minutes. The results indicated that dipping fillet in liquid smoke is a very effective treatment to prevent pathogenic bacteria and histamine forming bacteria.

The research aims to determine the phytochemical content such as phenolics, flavonoids, tannins, saponins, alkaloids, steroids, and triterpenoids found in the seaweed G. verrucosa. Seaweed G. verrucosa taken from the waters of North Sulawesi, Nain Village, North Minahasa, was extracted by maceration for 24 hours at room temperature, repeated 3 times using 96% ethanol solvent. The analysis in this study consisted of seven test parameters such as phenolic compounds, flavonoids, tannins, saponins, steroids, triterpenoids, and alkaloids. The results of the analysis showed that the secondary metabolites of G. verrucosa contained phytochemical compounds such as phenols, flavonoids, tannins, saponins, steroids, and alkaloids, and no triterpenoid compounds were found. It was concluded that G. verrucosa which grows in the waters of North Sulawesi, Nain Village, can function as functional food, and medicine because it has natural bioactive compounds. Keywords: Phytochemical content, Gracilaria verrucosa, secondary metabolites Abstrak Penelitian bertujuan mengetahui kandungan fitokimia seperti fenol, flavanoid, tanin, saponin, alkaloid, steroid, dan triterpenoid yang terdapat pada rumput laut G. verrucosa. Senyawa metabolit sekunder yang umum terdapat pada tanaman adalah senyawa terpenoid, alkaloid, fenolik, streroid, saponin, tannin. Rumput laut G. verrucosa yang di ambil dari perairan Sulawesi Utara Desa Nain Minahasa Utara di ekstraksi dengan cara maserasi selama 24 jam pada suhu ruang di ulangi sebanyak 3 kali menggunakan pelarut etanol 96 %. Analisis pada penelitian ini terdiri dari tujuh parameter pengujian seperti senyawa fenol, flavonoid, tanin, saponin, steroid, triterpenoid dan alkaloid. Hasil analisis menunjukan bahwa kandungan metabolit sekunder dari G. verrucosa mengandung senyawa fitokimia seperti fenol, flavonoid, tanin, saponin, steroid, alkaloid dan tidak ditemukan adanya senyawa triterpenoid. Hal ini disimpulkan bahwa G. verrucosa yang tumbuh di perairan Sulawesi utara Desa Nain dapat berfungsi sebagai pangan fungsional, obat-obatan karena memiliki senyawa bioaktif alami. Kata kunci: Kandungan fitokimia, Gracilaria verrucosa, metabolit sekunder

The objective of this study is to develop and evaluate the properties of smoked edible film (EF) composed of carrageenan, myofibril, and collagen. The smoked EF was prepared by incorporating 0.8% liquid smoke. The analysis focused on various parameters including pH, physical properties such as thickness, solubility, tensile strength, elongation percentage, and water vapor transmission rate (WVTR). Sensory evaluation was also conducted to assess the texture attributes of the coated product, including wateriness, firmness, elasticity, hardness, and juiciness. The findings revealed that the concentration of the ingredients influenced the thickness of the EF, with myofibril proteins exhibiting higher concentrations compared to carrageenan and collagen. Both collagen and myofibril demonstrated maximum solubility at a concentration of 6%, while carrageenan achieved optimal solubility at concentrations ranging from 2 to 2.5%. Carrageenan exhibited significantly higher tensile strength compared to myofibril and collagen, whereas collagen demonstrated greater elasticity than carrageenan and myofibril protein. Moreover, myofibril protein film exhibited a lower water vapor transmission rate compared to carrageenan and collagen films. In terms of sensory assessment, carrageenan displayed high elasticity and juiciness, while collagen and myofibril showed high firmness and hardness. All EFs showed better antioxidant activity compared to Trolox (EC50 < 95.57 µg/mL).

This research aimed to study the MSI of natural condiment cube coated with an edible coating, using the standard gravimetric method. Condiment pasta was performed into small cubes about 15 mm wide, dried, and coated with an edible coating made from carrageenan flour. Uncoated cube was used as a control sample. Samples of natural condiment cube were placed in a series of desiccators represented of a range of RH’s, and allowed to equilibrate, indicated by constant weight of samples. Water content of equilibrated sample was then measured as equilibrium moisture content (EMC). Five models of the equation were used in this experiment to fix Moisture sorption isotherm (MSI) data. The results show that natural condiment cube coated with edible coating has lower water content than control. The equilibrium moisture contents of natural condiment coated with edible coating ranged between 7.0 and 76 % dry basis. Henderson models showed best-fit values of regression coefficients with mean relative determination (MRD) of 8.21, and moisture sorption isotherm curved were sigmoid. In conclusion, edible coated were effectively used as a coating of natural condiment cube, stored in ambient temperatures only in RHs lower than 75%.

The aims of this studies were to prepare juice and raw condiment to be come semipreserve pastes, and to do microbial assessments on the both pastes during storing in refrigerator and ambient temperatures. For both pastes in refrigerator, samples were taken at 0, 2, 4, 5, 6, 8, 10, 15, 20, 25, and 30 days, and in ambient temperature samples were taken at 0, 1, 2, 3, 4, and 6 days. Assessment were done for TPC, total coliform and E. coli, Salmonella sp, Staphylococcus sp., Vibrio sp., pH and water content. The results shown that juice paste stored in refrigerator still good until 30 days (TPC 1,5x10⁴ CFU/g), and in ambient temperature still good until 6 days (2x10⁴ CFU/g). Condiment paste stored in refrigerator still good until 30 days (6.5x10³ CFU/g), and in ambient temperature still good until 6 days (1.17x10⁴ CFU/g). However, recommended that condiment paste stored in ambient temperature only until 4 days (7.3x10³CFU/g), while that juice paste until 5 days (7.8x10³CFU/g). There were no pathogenic bacteria found in all samples.