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Background Naso-ethmoidal meningoencephalocele is usually a congenital anomaly consisting of a protrusion of cerebral tissue and meninges into the ethmoidal labyrinth. The condition is a rare cause of structural epilepsy in dogs. We report the clinical presentation, surgical intervention, postoperative complications and outcome in a dog with drug resistant epilepsy secondary to a meningoencephalocele. Case presentation A 3.3-year-old male neutered Tamaskan Dog was referred for assessment of epileptic seizures secondary to a previously diagnosed left-sided naso-ethmoidal meningoencephalocele. The dog was drug resistant to medical management with phenobarbital, potassium bromide and levetiracetam. Surgical intervention was performed by a transfrontal craniotomy with resection of the meningoencephalocele and closure of the dural defect. Twenty-four hours after surgery the dog demonstrated progressive cervical hyperaesthesia caused by tension pneumocephalus and pneumorrhachis. Replacement of the fascial graft resulted in immediate resolution of the dog’s neurological signs. Within 5 months after surgery the dog progressively developed sneezing and haemorrhagic nasal discharge, caused by sinonasal aspergillosis. Systemic medical management with oral itraconazole (7 mg/kg orally q12h) was well-tolerated and resulted in resolution of the clinical signs. The itraconazole was tapered with no relapsing upper airway signs. The dog’s frequency of epileptic seizures was not affected by surgical resection of the meningoencephalocele. No treatment adjustments of the anti-epileptic medication have been necessary during the follow-up period of 15 months. Conclusions Surgical resection of the meningoencephalocele did not affect the seizure frequency of the dog. Further research on prognostic factors associated with surgical treatment of meningoencephaloceles in dogs is necessary. Careful monitoring for postsurgical complications allows prompt initiation of appropriate treatment.
Abtin Mojarradi; Sofie Van Meervenne; Alejandro Suarez-Bonnet; Steven De Decker. Diagnosis, treatment and postsurgical complications in a dog with epileptic seizures and a naso-ethmoidal meningoencephalocele. Acta Veterinaria Scandinavica 2021, 63, 1 -7.
AMA StyleAbtin Mojarradi, Sofie Van Meervenne, Alejandro Suarez-Bonnet, Steven De Decker. Diagnosis, treatment and postsurgical complications in a dog with epileptic seizures and a naso-ethmoidal meningoencephalocele. Acta Veterinaria Scandinavica. 2021; 63 (1):1-7.
Chicago/Turabian StyleAbtin Mojarradi; Sofie Van Meervenne; Alejandro Suarez-Bonnet; Steven De Decker. 2021. "Diagnosis, treatment and postsurgical complications in a dog with epileptic seizures and a naso-ethmoidal meningoencephalocele." Acta Veterinaria Scandinavica 63, no. 1: 1-7.
Canine gastric carcinoma (CGC) affects both sexes in relatively equal proportions, with a mean age of nine years, and the highest frequency in Staffordshire bull terriers. The most common histological subtype in 149 CGC cases was the undifferentiated carcinoma. CGCs were associated with increased chronic inflammation parameters and a greater chronic inflammatory score when Helicobacter spp. were present. Understanding the molecular pathways of gastric carcinoma is challenging. All markers showed variable expression for each subtype. Expression of the cell cycle regulator 14-3-3σ was positive in undifferentiated, tubular and papillary carcinomas. This demonstrates that 14-3-3σ could serve as an immunohistochemical marker in routine diagnosis and that mucinous, papillary and signet-ring cell (SRC) carcinomas follow a 14-3-3σ independent pathway. p16, another cell cycle regulator, showed increased expression in mucinous and SRC carcinomas. Expression of the adhesion molecules E-cadherin and CD44 appear context-dependent, with switching within tumor emboli potentially playing an important role in tumor cell survival, during invasion and metastasis. Within neoplastic emboli, acinar structures lacked expression of all markers, suggesting an independent molecular pathway that requires further investigation. These findings demonstrate similarities and differences between dogs and humans, albeit further clinicopathological data and molecular analysis are required.
Alexandros Hardas; Alejandro Suárez-Bonnet; Sam Beck; William Becker; Gustavo Ramírez; Simon Priestnall. Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart. Animals 2021, 11, 1409 .
AMA StyleAlexandros Hardas, Alejandro Suárez-Bonnet, Sam Beck, William Becker, Gustavo Ramírez, Simon Priestnall. Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart. Animals. 2021; 11 (5):1409.
Chicago/Turabian StyleAlexandros Hardas; Alejandro Suárez-Bonnet; Sam Beck; William Becker; Gustavo Ramírez; Simon Priestnall. 2021. "Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart." Animals 11, no. 5: 1409.
A 2‐year‐old male neutered domestic shorthair cat underwent investigations for acute onset of lethargy, hyporexia, and cough. Computed tomography of the thorax identified a large mass‐like lesion in the left cranial lung lobe and bilateral pleural effusion. Thoracotomy and left cranial lung lobectomy were performed. Histopathology of the pulmonary mass was consistent with a localized Toxoplasma gondii pneumonia, confirmed by positive polymerase chain reaction on the affected lung lobe. After adjunctive medical management with a 28‐day course of clindamycin (12.5 mg/kg PO q12h), clinical signs resolved and repeat thoracic radiographs documented no abnormalities. The cat remains clinically well 1 year after surgery.
Myles McKenna; Monica Augusto; Alejandro Suárez‐Bonnet; Ella Fitzgerald. Pulmonary mass‐like lesion caused by Toxoplasma gondii in a domestic shorthair cat. Journal of Veterinary Internal Medicine 2021, 35, 1547 -1550.
AMA StyleMyles McKenna, Monica Augusto, Alejandro Suárez‐Bonnet, Ella Fitzgerald. Pulmonary mass‐like lesion caused by Toxoplasma gondii in a domestic shorthair cat. Journal of Veterinary Internal Medicine. 2021; 35 (3):1547-1550.
Chicago/Turabian StyleMyles McKenna; Monica Augusto; Alejandro Suárez‐Bonnet; Ella Fitzgerald. 2021. "Pulmonary mass‐like lesion caused by Toxoplasma gondii in a domestic shorthair cat." Journal of Veterinary Internal Medicine 35, no. 3: 1547-1550.
This study aimed to describe the anatomic features of the normal head of the Komodo dragon (Varanus komodoensis) identified by computed tomography. CT images were obtained in two dragons using a helical CT scanner. All sections were displayed with a bone and soft tissue windows setting. Head reconstructed, and maximum intensity projection images were obtained to enhance bony structures. After CT imaging, the images were compared with other studies and reptile anatomy textbooks to facilitate the interpretation of the CT images. Anatomic details of the head of the Komodo dragon were identified according to the CT density characteristics of the different organic tissues. This information is intended to be a useful initial anatomic reference in interpreting clinical CT imaging studies of the head and associated structures in live Komodo dragons.
Sara Pérez; Mario Encinoso; Juan Corbera; Manuel Morales; Alberto Arencibia; Eligia González-Rodríguez; Soraya Déniz; Carlos Melián; Alejandro Suárez-Bonnet; José Jaber. Cranial Structure of Varanus komodoensis as Revealed by Computed-Tomographic Imaging. Animals 2021, 11, 1078 .
AMA StyleSara Pérez, Mario Encinoso, Juan Corbera, Manuel Morales, Alberto Arencibia, Eligia González-Rodríguez, Soraya Déniz, Carlos Melián, Alejandro Suárez-Bonnet, José Jaber. Cranial Structure of Varanus komodoensis as Revealed by Computed-Tomographic Imaging. Animals. 2021; 11 (4):1078.
Chicago/Turabian StyleSara Pérez; Mario Encinoso; Juan Corbera; Manuel Morales; Alberto Arencibia; Eligia González-Rodríguez; Soraya Déniz; Carlos Melián; Alejandro Suárez-Bonnet; José Jaber. 2021. "Cranial Structure of Varanus komodoensis as Revealed by Computed-Tomographic Imaging." Animals 11, no. 4: 1078.
This study describes the clinical and pathological characteristics of cutaneous spindle cell squamous cell carcinoma (SCSCC) in 18 cats. The average age of the cats was 11.8 ± 2.7 years, and all tumors were located in the facial skin, mainly affecting the pinna (13/18, 72%), followed by the periorbital area (4/18, 22%) and the dorsal muzzle (1/18, 6%). Tumors were composed of fusiform neoplastic cells with moderate atypia arranged in solid sheets or fascicles with foci of squamous differentiation. A panel of antibodies against cytokeratins, vimentin, S-100 protein, NSE, GFAP, Melan A, SMA, desmin, CD18, CD31, and p63 was used to help differentiate SCSCC from other spindle cell malignancies. SCSCCs expressed CK5/6 (17/18, 94%), AE1/AE3 (15/18, 83%), and p63 protein (18/18, 100%), but there was no immunolabeling for CK8/18. A role for sunlight exposure in the pathogenesis of the tumors was suggested by changes indicative of actinic keratosis, the location of the tumors in dorsal areas, and the absence of histomorphologic features of papillomavirus infection. Recurrence was not recorded in 14/18 cases (78%) during a follow-up period of 7 to 25 months. Three of 18 (17%) tumors recurred or led to humane euthanasia due to local progression, and one case (5%) had regional lymph node metastasis. Clinical outcome varied with cutaneous location, mitotic count, and invasion of surgical margins; thus, SCSCCs with a more aggressive behavior were located in the periorbital area (4/4 cases), had ≥14 mitoses in 10 high-power fields (2.37 mm2) (4/4 cases), and showed invasion of surgical margins (3/4 cases).
Francisco Rodríguez Guisado; Alejandro Suárez-Bonnet; Gustavo A. Ramírez. Cutaneous Spindle Cell Squamous Cell Carcinoma in Cats: Clinical, Histological, and Immunohistochemical Study. Veterinary Pathology 2021, 58, 503 -507.
AMA StyleFrancisco Rodríguez Guisado, Alejandro Suárez-Bonnet, Gustavo A. Ramírez. Cutaneous Spindle Cell Squamous Cell Carcinoma in Cats: Clinical, Histological, and Immunohistochemical Study. Veterinary Pathology. 2021; 58 (3):503-507.
Chicago/Turabian StyleFrancisco Rodríguez Guisado; Alejandro Suárez-Bonnet; Gustavo A. Ramírez. 2021. "Cutaneous Spindle Cell Squamous Cell Carcinoma in Cats: Clinical, Histological, and Immunohistochemical Study." Veterinary Pathology 58, no. 3: 503-507.
Many tumour cells show dependence on exogenous serine and dietary serine and glycine starvation can inhibit the growth of these cancers and extend survival in mice. However, numerous mechanisms promote resistance to this therapeutic approach, including enhanced expression of the de novo serine synthesis pathway (SSP) enzymes or activation of oncogenes that drive enhanced serine synthesis. Here we show that inhibition of PHGDH, the first step in the SSP, cooperates with serine and glycine depletion to inhibit one-carbon metabolism and cancer growth. In vitro, inhibition of PHGDH combined with serine starvation leads to a defect in global protein synthesis, which blocks the activation of an ATF-4 response and more broadly impacts the protective stress response to amino acid depletion. In vivo, the combination of diet and inhibitor shows therapeutic efficacy against tumours that are resistant to diet or drug alone, with evidence of reduced one-carbon availability. However, the defect in ATF4-response seen in vitro following complete depletion of available serine is not seen in mice, where dietary serine and glycine depletion and treatment with the PHGDH inhibitor lower but do not eliminate serine. Our results indicate that inhibition of PHGDH will augment the therapeutic efficacy of a serine depleted diet.
Mylène Tajan; Marc Hennequart; Eric C. Cheung; Fabio Zani; Andreas K. Hock; Nathalie Legrave; Oliver D. K. Maddocks; Rachel A. Ridgway; Dimitris Athineos; Alejandro Suárez-Bonnet; Robert L. Ludwig; Laura Novellasdemunt; Nikolaos Angelis; Vivian S. W. Li; Georgios Vlachogiannis; Nicola Valeri; Nello Mainolfi; Vipin Suri; Adam Friedman; Mark Manfredi; Karen Blyth; Owen J. Sansom; Karen H. Vousden. Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy. Nature Communications 2021, 12, 1 -16.
AMA StyleMylène Tajan, Marc Hennequart, Eric C. Cheung, Fabio Zani, Andreas K. Hock, Nathalie Legrave, Oliver D. K. Maddocks, Rachel A. Ridgway, Dimitris Athineos, Alejandro Suárez-Bonnet, Robert L. Ludwig, Laura Novellasdemunt, Nikolaos Angelis, Vivian S. W. Li, Georgios Vlachogiannis, Nicola Valeri, Nello Mainolfi, Vipin Suri, Adam Friedman, Mark Manfredi, Karen Blyth, Owen J. Sansom, Karen H. Vousden. Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy. Nature Communications. 2021; 12 (1):1-16.
Chicago/Turabian StyleMylène Tajan; Marc Hennequart; Eric C. Cheung; Fabio Zani; Andreas K. Hock; Nathalie Legrave; Oliver D. K. Maddocks; Rachel A. Ridgway; Dimitris Athineos; Alejandro Suárez-Bonnet; Robert L. Ludwig; Laura Novellasdemunt; Nikolaos Angelis; Vivian S. W. Li; Georgios Vlachogiannis; Nicola Valeri; Nello Mainolfi; Vipin Suri; Adam Friedman; Mark Manfredi; Karen Blyth; Owen J. Sansom; Karen H. Vousden. 2021. "Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy." Nature Communications 12, no. 1: 1-16.
An 11‐year‐old male neutered Boxer presented for evaluation of an external ear canal mass, postoperatively diagnosed as a benign inflammatory lesion. Preoperative staging of the ear mass identified a 10‐cm mass affecting the majority of the left lateral hepatic lobe, with inconclusive cytology. A left lateral liver lobectomy was performed. Histopathology revealed the hepatic parenchyma was largely effaced and replaced by a myriad of small calibre tubules lined by well‐differentiated biliary epithelium (cytokeratin19 and cytokeratin7 positive (CK19+, CK7+) supported by abundant fibrosis. Small cords and islands of hepatocytes (hepatocyte paraffin1 positive) (HepPar1+) were entrapped throughout these bile ducts. Analysis of the proliferation index via antigen KI‐67 (Ki67) immunohistochemistry confirmed lack of proliferative activity. Histological and immunohistochemical features were consistent with a ductal plate malformation (DPM). This is the first time a DPM of this size has been described on a computed tomography (CT) scan.
Heidi Kaniok; Esteban Gonzalez‐Gasch; Elizabeth Alloway; Alejandro Suarez‐Bonnet. An unusual presentation of a hepatic ductal malformation in a Boxer dog. Veterinary Record Case Reports 2021, 9, 1 .
AMA StyleHeidi Kaniok, Esteban Gonzalez‐Gasch, Elizabeth Alloway, Alejandro Suarez‐Bonnet. An unusual presentation of a hepatic ductal malformation in a Boxer dog. Veterinary Record Case Reports. 2021; 9 (1):1.
Chicago/Turabian StyleHeidi Kaniok; Esteban Gonzalez‐Gasch; Elizabeth Alloway; Alejandro Suarez‐Bonnet. 2021. "An unusual presentation of a hepatic ductal malformation in a Boxer dog." Veterinary Record Case Reports 9, no. 1: 1.
Canine tonsillar polyps are uncommon. We describe 14 tonsillar polyps in dogs and review their classification and pathogenesis. All dogs were adult (3–13 years old). Females (10/14) were more affected than males (4/14). Most of the lesions were asymptomatic (10/14). All lesions were unilateral, pedunculated (9/14), or sessile (5/14), with a smooth (12/14) or papillary/verrucous surface (2/14). Histologically, polyps consisted of benign proliferation of lymphatic vessels, blood vessels, fibrous tissue, and lymphoid tissue in variable proportions, with occasional adipose tissue (4/14). According to the main stromal components, polyps were categorized as lymphangiomatous (5/14), lymphangiolipomatous (2/14), lymphangiofibromatous (2/14), angiofibromatous (1/14), angiofibrolipomatous (1/14), lymphoid (2/14), and myxomatous (1/14). As the pathogenesis of these polyps remains unclear, we propose to replace the term inflammatory tonsillar polyp by a morphological diagnosis based on the stromal characteristics of the lesions. Simple surgical excision was curative in the 9 cases with available follow-up information.
Jéssica Molín; Miquel Vilafranca; Alejandro Suárez-Bonnet; Jaume Altimira; Gustavo A. Ramírez. Canine Tonsillar Polyps: Characteristics, Classification, and Review of the Pathogenesis. Veterinary Pathology 2020, 58, 136 -141.
AMA StyleJéssica Molín, Miquel Vilafranca, Alejandro Suárez-Bonnet, Jaume Altimira, Gustavo A. Ramírez. Canine Tonsillar Polyps: Characteristics, Classification, and Review of the Pathogenesis. Veterinary Pathology. 2020; 58 (1):136-141.
Chicago/Turabian StyleJéssica Molín; Miquel Vilafranca; Alejandro Suárez-Bonnet; Jaume Altimira; Gustavo A. Ramírez. 2020. "Canine Tonsillar Polyps: Characteristics, Classification, and Review of the Pathogenesis." Veterinary Pathology 58, no. 1: 136-141.
Tuberculosis (TB) is a leading cause of mortality due to infectious disease, but the factors determining disease progression are unclear. Transcriptional signatures associated with type I IFN signalling and neutrophilic inflammation were shown to correlate with disease severity in mouse models of TB. Here we show that similar transcriptional signatures correlate with increased bacterial loads and exacerbate pathology during Mycobacterium tuberculosis infection upon GM-CSF blockade. Loss of GM-CSF signalling or genetic susceptibility to TB (C3HeB/FeJ mice) result in type I IFN-induced neutrophil extracellular trap (NET) formation that promotes bacterial growth and promotes disease severity. Consistently, NETs are present in necrotic lung lesions of TB patients responding poorly to antibiotic therapy, supporting the role of NETs in a late stage of TB pathogenesis. Our findings reveal an important cytokine-based innate immune effector network with a central role in determining the outcome of M. tuberculosis infection.
Lúcia Moreira-Teixeira; Philippa J. Stimpson; Evangelos Stavropoulos; Sabelo Hadebe; Probir Chakravarty; Marianna Ioannou; Iker Valle Aramburu; Eleanor Herbert; Simon L. Priestnall; Alejandro Suarez-Bonnet; Jeremy Sousa; Kaori L. Fonseca; Qian Wang; Sergo Vashakidze; Paula Rodríguez-Martínez; Cristina Vilaplana; Margarida Saraiva; Venizelos Papayannopoulos; Anne O’Garra. Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis. Nature Communications 2020, 11, 1 -18.
AMA StyleLúcia Moreira-Teixeira, Philippa J. Stimpson, Evangelos Stavropoulos, Sabelo Hadebe, Probir Chakravarty, Marianna Ioannou, Iker Valle Aramburu, Eleanor Herbert, Simon L. Priestnall, Alejandro Suarez-Bonnet, Jeremy Sousa, Kaori L. Fonseca, Qian Wang, Sergo Vashakidze, Paula Rodríguez-Martínez, Cristina Vilaplana, Margarida Saraiva, Venizelos Papayannopoulos, Anne O’Garra. Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis. Nature Communications. 2020; 11 (1):1-18.
Chicago/Turabian StyleLúcia Moreira-Teixeira; Philippa J. Stimpson; Evangelos Stavropoulos; Sabelo Hadebe; Probir Chakravarty; Marianna Ioannou; Iker Valle Aramburu; Eleanor Herbert; Simon L. Priestnall; Alejandro Suarez-Bonnet; Jeremy Sousa; Kaori L. Fonseca; Qian Wang; Sergo Vashakidze; Paula Rodríguez-Martínez; Cristina Vilaplana; Margarida Saraiva; Venizelos Papayannopoulos; Anne O’Garra. 2020. "Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis." Nature Communications 11, no. 1: 1-18.
An 11‐year‐old male intact Staffordshire Bull terrier was referred for diabetic ketoacidosis. Abdominal ultrasonographic examination revealed a 5 cm × 2 cm intraluminal caecal mass‐like structure. Exploratory laparotomy and typhlectomy were subsequently performed. Histopathology of the caecal mass‐like structure was consistent with a caecal mucocoele, defined as a cystic dilation of the caecal lumen with stasis of mucus. This lesion has been previously described in humans, where it is termed an appendiceal mucocoele. The patient was euthanased 58 days post‐operatively due to unrelated diabetic complications.
M. McKenna; A. Suárez‐Bonnet; K. Smith; S. Stewart. Diagnosis and treatment of a caecal mucocoele in a dog. Journal of Small Animal Practice 2020, 62, 305 -309.
AMA StyleM. McKenna, A. Suárez‐Bonnet, K. Smith, S. Stewart. Diagnosis and treatment of a caecal mucocoele in a dog. Journal of Small Animal Practice. 2020; 62 (4):305-309.
Chicago/Turabian StyleM. McKenna; A. Suárez‐Bonnet; K. Smith; S. Stewart. 2020. "Diagnosis and treatment of a caecal mucocoele in a dog." Journal of Small Animal Practice 62, no. 4: 305-309.
An 8-year-old neutered male French Bulldog was presented with a 2-day history of intermittent vomiting, reduced appetite and recent rapid development of multiple cutaneous masses over the head and neck regions. On presentation, the patient had a moderate volume of pericardial and bilateral pleural effusion. Echocardiography demonstrated irregular, heterogeneous thickening of the walls of the right ventricle and right atrium, consistent with infiltrative intramyocardial disease. Cytological examination of fine needle aspirates from one of the cutaneous masses confirmed a mast cell tumour. Pericardial fluid analysis revealed a haemorrhagic neoplastic effusion due to mast cell neoplasia. Histopathological and immunohistochemical examination of tissues obtained post mortem confirmed a high-grade cutaneous mast cell tumour with metastasis to the heart, pericardium, mediastinum and spleen. No metastatic disease was present in the submandibular lymph nodes or liver. Immunohistochemistry demonstrated KIT staining pattern 2. There was strong nuclear Ki67 labelling in an average of 65.0 cells per grid and an average of three positive AgNORs per nucleus in neoplastic cells. Polymerase chain reaction for the activating duplication mutation in exons 8 and 11 of c-Kit were negative. To the authors’ knowledge, this is the first report of a canine cutaneous mast cell tumour associated with neoplastic pericardial effusion and widespread intrathoracic metastasis.
Andrew D Yale; Balazs Szladovits; Anneliese J Stell; Scott D Fitzgerald; Simon L Priestnall; Alejandro Suarez-Bonnet. High-Grade Cutaneous Mast Cell Tumour with Widespread Intrathoracic Metastasis and Neoplastic Pericardial Effusion in a Dog. Journal of Comparative Pathology 2020, 180, 29 -34.
AMA StyleAndrew D Yale, Balazs Szladovits, Anneliese J Stell, Scott D Fitzgerald, Simon L Priestnall, Alejandro Suarez-Bonnet. High-Grade Cutaneous Mast Cell Tumour with Widespread Intrathoracic Metastasis and Neoplastic Pericardial Effusion in a Dog. Journal of Comparative Pathology. 2020; 180 ():29-34.
Chicago/Turabian StyleAndrew D Yale; Balazs Szladovits; Anneliese J Stell; Scott D Fitzgerald; Simon L Priestnall; Alejandro Suarez-Bonnet. 2020. "High-Grade Cutaneous Mast Cell Tumour with Widespread Intrathoracic Metastasis and Neoplastic Pericardial Effusion in a Dog." Journal of Comparative Pathology 180, no. : 29-34.
We present a unique case of metastatic cholangiocarcinoma with concurrent abdominal cestodiasis in an African green monkey (Chlorocebus pygerythrus) that presented with respiratory insufficiency and abdominal discomfort. There were multiple white–grey masses in the liver and colonic serosa alongside intra-abdominal parasitic cysts. Histopathologically, the liver masses were composed of poorly-differentiated epithelial cells that formed densely cellular solid areas and trabeculae. The neoplastic cells were strongly immunopositive for CK7 but negative for Hep-Par1 antigen, which confirmed a diagnosis of cholangiocarcinoma. Interestingly, there was strong and diffuse neoexpression in the tumour of the cell cycle regulator 14-3-3σ, which is not constitutively expressed in normal liver. There was aberrantly strong expression of E-cadherin, a key cell–cell adhesion protein, in neoplastic cells with evidence of cytoplasmic internalization. This is the first immunohistochemical analysis of 14-3-3σ and E-cadherin in a liver neoplasm in an animal species and the use of these markers requires further investigation in animal liver neoplasms.
A. Suárez-Bonnet; S.L. Priestnall; Gustavo A. Ramirez; J. Molín; J.R. Jaber. Aberrant Expression of Cell Cycle Regulator 14-3-3-σ and E-Cadherin in a Metastatic Cholangiocarcinoma in a Vervet Monkey (Chlorocebus pygerythrus). Journal of Comparative Pathology 2020, 179, 25 -30.
AMA StyleA. Suárez-Bonnet, S.L. Priestnall, Gustavo A. Ramirez, J. Molín, J.R. Jaber. Aberrant Expression of Cell Cycle Regulator 14-3-3-σ and E-Cadherin in a Metastatic Cholangiocarcinoma in a Vervet Monkey (Chlorocebus pygerythrus). Journal of Comparative Pathology. 2020; 179 ():25-30.
Chicago/Turabian StyleA. Suárez-Bonnet; S.L. Priestnall; Gustavo A. Ramirez; J. Molín; J.R. Jaber. 2020. "Aberrant Expression of Cell Cycle Regulator 14-3-3-σ and E-Cadherin in a Metastatic Cholangiocarcinoma in a Vervet Monkey (Chlorocebus pygerythrus)." Journal of Comparative Pathology 179, no. : 25-30.
Equine penile squamous cell carcinoma (EpSCC) is a relatively common cutaneous neoplasm with a poor prognosis. In this study, we aimed to determine the protein expression and colocalisation of FRA1, c-Myc, Cyclin D1, and MMP7 in normal (NT), tumour (T), hyperplastic epidermis and/or squamous papilloma (Hyp/Pap), poorly-differentiated (PDSCC), or well-differentiated (WDSCC) EpSCC using a tissue array approach. Further objectives were to correlate protein expression to (i) levels of inflammation, using a convolutional neural network (ii) equine papillomavirus 2 (EcPV2) infection, detected using PCR amplification. We found an increase in expression of FRA1 in EpSCC compared to NT samples. c-Myc expression was higher in Hyp/Pap and WDSCC but not PDSCC whereas MMP7 was reduced in WDSCC compared with NT. There was a significant increase in the global intersection coefficient (GIC) of FRA1 with MMP7, c-Myc, and Cyclin D1 in EpSCC. Conversely, GIC for MMP7 with c-Myc was reduced in EpSCC tissue. Inflammation was positively associated with EcPV2 infection in both NT and EpSCC but not Hyp/Pap. Changes in protein expression could be correlated with EcPV2 for Cyclin D1 and c-Myc. Our results evaluate novel biomarkers of EpSCC and a putative correlation between the expression of biomarkers, EcPV2 infection and inflammation.
Callum Arthurs; Alejandro Suarez-Bonnet; Claire Willis; Boyu Xie; Natalie Machulla; Tim S. Mair; Kevin Cao; Michael Millar; Christopher Thrasivoulou; Simon L. Priestnall; Aamir Ahmed. Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2. Scientific Reports 2020, 10, 1 -13.
AMA StyleCallum Arthurs, Alejandro Suarez-Bonnet, Claire Willis, Boyu Xie, Natalie Machulla, Tim S. Mair, Kevin Cao, Michael Millar, Christopher Thrasivoulou, Simon L. Priestnall, Aamir Ahmed. Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2. Scientific Reports. 2020; 10 (1):1-13.
Chicago/Turabian StyleCallum Arthurs; Alejandro Suarez-Bonnet; Claire Willis; Boyu Xie; Natalie Machulla; Tim S. Mair; Kevin Cao; Michael Millar; Christopher Thrasivoulou; Simon L. Priestnall; Aamir Ahmed. 2020. "Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2." Scientific Reports 10, no. 1: 1-13.
Coagulase-positive Staphylococci (CoPS) can exist as commensals in humans, companion and food-producing animals, but can cause severe or even lethal diseases. Exchange of these bacteria between humans and animals has been described. Special attention has been focused on Methicillin-Resistant Staphylococcus aureus, but other CoPS can also represent an important threat. In addition to significant antimicrobial resistance, these bacteria may carry a plethora of virulence factors - molecules that allow bacteria to establish on or within a host and increase their ability to cause disease. These virulence factors have been widely described in S. aureus but information about other species of CoPS is scarce. The aim of this paper is to review the recent literature about the virulence factors of non-aureus CoPS of animal origin. Their possible effects on human health are also described. The role and prevalence of different virulence factors including leukocidins, hemolysins, adhesins, enterotoxins, exfoliative and toxic shock syndrome toxins as well as superantigen-like proteins are addressed. The effect of these virulence factors on human health is also described. The possibility of misdiagnosis of species of CoPS has been demonstrated in human clinical samples. Prevalence of zoonotic infections could be higher than thought and medical laboratories should be aware of these other staphylococcal species. In keeping with the ‘One Health’ approach to animal and human disease, medical professionals, veterinarians and health workers should be aware of the risks derived from exposure to these bacteria in people in close contact with animals, including pet owners, farmers and veterinarians themselves.
Margarita González-Martín; Juan Alberto Corbera; Alejandro Suárez-Bonnet; María Teresa Tejedor-Junco. Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus. Veterinary Quarterly 2020, 40, 118 -131.
AMA StyleMargarita González-Martín, Juan Alberto Corbera, Alejandro Suárez-Bonnet, María Teresa Tejedor-Junco. Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus. Veterinary Quarterly. 2020; 40 (1):118-131.
Chicago/Turabian StyleMargarita González-Martín; Juan Alberto Corbera; Alejandro Suárez-Bonnet; María Teresa Tejedor-Junco. 2020. "Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus." Veterinary Quarterly 40, no. 1: 118-131.
A 2-year-old female neutered crossbreed dog was presented for evaluation of a 3-day history of haematemesis, melaena and hyporexia. The dog had been imported from a rescue centre in Hungary 4 months prior to presentation. Abdominal CT revealed the presence of a 3 cm×3 cm×4 cm diameter heterogenous intraluminal mass lesion in the gastric cardia, surrounding the ostium of the cardia and continuous with the distal oesophagus. The mass lesion was subsequently surgically resected. Histopathology of the gastric mass lesion was consistent with a Spirocerca lupi granuloma. The patient unfortunately developed a pyothorax and suffered cardiopulmonary arrest resulting in death 2 days postoperatively. To the authors’ knowledge, this is the first reported case of clinical spirocercosis reported in a dog in the UK.
Myles McKenna; Poppy Charlotte Bristow; Alejandro Suarez‐Bonnet; Mark Fox; Barbara Glanemann. Clinical spirocercosis in a dog in the UK. Veterinary Record Case Reports 2019, 7, e000828 .
AMA StyleMyles McKenna, Poppy Charlotte Bristow, Alejandro Suarez‐Bonnet, Mark Fox, Barbara Glanemann. Clinical spirocercosis in a dog in the UK. Veterinary Record Case Reports. 2019; 7 (3):e000828.
Chicago/Turabian StyleMyles McKenna; Poppy Charlotte Bristow; Alejandro Suarez‐Bonnet; Mark Fox; Barbara Glanemann. 2019. "Clinical spirocercosis in a dog in the UK." Veterinary Record Case Reports 7, no. 3: e000828.
Background The cause of low serum vitamin D concentrations in dogs with chronic inflammatory enteropathy (CIE) is not well understood. Objective Improve understanding of pathogenesis of low serum vitamin D concentrations in dogs with CIE by comparing several clinical, clinicopathologic, and histologic variables between CIE dogs with low and normal serum 25‐hydroxyvitamin D concentrations (25[OH]D). Animals Fifteen dogs with CIE and low serum 25[OH]D concentrations; 15 dogs with CIE and normal serum 25(OH)D concentrations. Methods Prospective cohort study. Clinical and clinicopathologic variables were compared between groups. Correlations between serum 25(OH)D concentration and histopathologic variables were assessed. Results Dogs with CIE and low serum 25(OH)D concentrations had higher canine chronic enteropathy clinical activity index scores (P = .003), lower serum α‐tocopherol (P < .001), cholesterol (P < .001), and albumin (P < .001) concentrations and higher serum C‐reactive protein (P = .004) concentrations compared to CIE dogs with normal serum 25(OH)D concentrations. Serum concentrations of vitamin D‐binding protein (VDBP) were not different between groups (P = .91). Duodenal morphologic and inflammatory histopathological scores (P = .002 and P = .004, respectively) and total histopathological scores in duodenum and combined duodenum and ileum negatively correlated with serum 25(OH)D concentration. Conclusions and Clinical Importance The pathogenesis of low serum vitamin D concentrations in dogs with CIE is likely multifactorial. Fat malabsorption deserves further study in dogs with low serum vitamin D concentration and CIE. Loss of VDBP does not appear to be an important cause of low serum vitamin D concentration in dogs with CIE.
Sara A. Wennogle; Simon L. Priestnall; Alejandro Suárez‐Bonnet; Craig B. Webb. Comparison of clinical, clinicopathologic, and histologic variables in dogs with chronic inflammatory enteropathy and low or normal serum 25‐hydroxycholecalciferol concentrations. Journal of Veterinary Internal Medicine 2019, 33, 1995 -2004.
AMA StyleSara A. Wennogle, Simon L. Priestnall, Alejandro Suárez‐Bonnet, Craig B. Webb. Comparison of clinical, clinicopathologic, and histologic variables in dogs with chronic inflammatory enteropathy and low or normal serum 25‐hydroxycholecalciferol concentrations. Journal of Veterinary Internal Medicine. 2019; 33 (5):1995-2004.
Chicago/Turabian StyleSara A. Wennogle; Simon L. Priestnall; Alejandro Suárez‐Bonnet; Craig B. Webb. 2019. "Comparison of clinical, clinicopathologic, and histologic variables in dogs with chronic inflammatory enteropathy and low or normal serum 25‐hydroxycholecalciferol concentrations." Journal of Veterinary Internal Medicine 33, no. 5: 1995-2004.
Background Lymphatic endothelial cell (LEC) immunohistochemical markers have identified intestinal lymphatic vasculature abnormalities in humans with inflammatory bowel disease, but have not been used to evaluate intestinal lymphatic vasculature in a group of dogs with chronic inflammatory enteropathy (CIE). Objectives To utilize LEC markers to identify and measure intestinal lymphatic vasculature in endoscopic biopsy samples of CIE dogs. To evaluate whether measured lymphatic vasculature variables correlate with serum albumin concentrations. Animals Twenty‐four dogs with CIE; n = 13, serum albumin concentration <2.5 g/dL (CIE‐protein‐losing enteropathy [PLE]), n = 11, serum albumin concentration ≥2.5 g/dL (CIE‐N). Methods Prospective study. Lymphatic endothelial cell immunolabeling with Prox‐1 and LYVE‐1 performed on endoscopic biopsy samples from 24 dogs with CIE. Duodenal and ileal villous lacteal width (VLW) and proprial mucosal lacteal width (MLW) were determined for each case and analyzed for correlation with serum albumin concentration. Lacteal dilatation scores using routine H&E histopathology were assessed for correlation with immunohistochemistry (IHC)‐calculated VLW and MLW. Results Lower serum albumin concentrations were correlated with increased VLW (rho = −.4644; P = .02) and MLW (rho = −.6514; P < .001) in the ileum. Lymphatic endothelial cell IHC identified presumptive proprial mucosal lymphangiectasia in some dogs that was not recognized with routine H&E staining. Lacteal dilatation scores were correlated with VLW in duodenum (rho = .4634; P = .02) and ileum (rho = .5292; P = .008), but did not correlate with MLW. Conclusions and Clinical Importance Lymphatic endothelial cell immunolabeling identified presumptive proprial mucosal lymphangiectasia in CIE dogs, particularly in the ileum of hypoalbuminemic dogs. Routine evaluation of villous lacteals likely underestimates abnormalities of the lymphatic vasculature in dogs with CIE.
Sara A. Wennogle; Simon L. Priestnall; Alejandro Suárez‐Bonnet; Sirikul Soontararak; Craig B. Webb. Lymphatic endothelial cell immunohistochemical markers for evaluation of the intestinal lymphatic vasculature in dogs with chronic inflammatory enteropathy. Journal of Veterinary Internal Medicine 2019, 33, 1669 -1676.
AMA StyleSara A. Wennogle, Simon L. Priestnall, Alejandro Suárez‐Bonnet, Sirikul Soontararak, Craig B. Webb. Lymphatic endothelial cell immunohistochemical markers for evaluation of the intestinal lymphatic vasculature in dogs with chronic inflammatory enteropathy. Journal of Veterinary Internal Medicine. 2019; 33 (4):1669-1676.
Chicago/Turabian StyleSara A. Wennogle; Simon L. Priestnall; Alejandro Suárez‐Bonnet; Sirikul Soontararak; Craig B. Webb. 2019. "Lymphatic endothelial cell immunohistochemical markers for evaluation of the intestinal lymphatic vasculature in dogs with chronic inflammatory enteropathy." Journal of Veterinary Internal Medicine 33, no. 4: 1669-1676.
Our study provides evidence of molecular abnormalities in ePSCC similar to those reported for human PSCC. The occurrence of EMT at the IF is a common event in ePSCC. Naturally occurring ePSCC could serve as a valuable preclinical animal model to explore upcoming therapeutic options for hPSCC.
Alejandro Suárez-Bonnet; Claire Willis; Rachel Pittaway; Ken Smith; Tim Mair; Simon L. Priestnall. Molecular carcinogenesis in equine penile cancer: A potential animal model for human penile cancer. Urologic Oncology: Seminars and Original Investigations 2018, 36, 532.e9 -532.e18.
AMA StyleAlejandro Suárez-Bonnet, Claire Willis, Rachel Pittaway, Ken Smith, Tim Mair, Simon L. Priestnall. Molecular carcinogenesis in equine penile cancer: A potential animal model for human penile cancer. Urologic Oncology: Seminars and Original Investigations. 2018; 36 (12):532.e9-532.e18.
Chicago/Turabian StyleAlejandro Suárez-Bonnet; Claire Willis; Rachel Pittaway; Ken Smith; Tim Mair; Simon L. Priestnall. 2018. "Molecular carcinogenesis in equine penile cancer: A potential animal model for human penile cancer." Urologic Oncology: Seminars and Original Investigations 36, no. 12: 532.e9-532.e18.
Divergent differentiation is encountered frequently within human malignant peripheral nerve sheath tumours (MPNSTs). The new component is often a rhabdomyosarcoma, but in animals this specific form of divergent differentiation within MPNSTs has only been reported once (in a dog). Incisional wedge biopsy of a locally extensive, ventral abdominal wall mass, which extended from the dermis to the subcutis, from a 12-year-old female domestic shorthaired cat, was performed. The tissue was examined with routine haematoxylin and eosin staining and immunohistochemical methods. A malignant neoplasm with spindle and polygonal cell components and progression towards a rhabdomyosarcomatous phenotype was observed. Both neoplastic cell populations exhibited strong expression of vimentin and there was multifocal expression of S100 and desmin. There was strong cytoplasmic labelling for α-sarcomeric actin and muscle actin and weak labelling for myoglobin within the cells positive for desmin. There was multifocal positive nuclear labelling for myogenin. Glial fibrillary acidic protein, α-smooth muscle actin, microphthalmia-associated transcription factor and melanoma antigen recognized by T cells were not expressed. Microscopical features, aided by immunohistochemistry, identified a MPNST with progression towards a rhabdomyosarcomatous phenotype, a so-called 'triton tumour'. A Schwann cell component could account for the divergent patterns of growth, given the plasticity of the neural crest. Nerve sheath tumours have been reported in the skin and subcutis of cats and are a differential diagnosis of feline cutaneous spindle cell neoplasms.
A.L. Stoll; A. Suárez-Bonnet; B.A. Summers; S.L. Priestnall. Malignant Cutaneous Peripheral Nerve Sheath Tumour with Rhabdomyosarcomatous Differentiation (Triton Tumour) in a Domestic Cat. Journal of Comparative Pathology 2018, 165, 1 -5.
AMA StyleA.L. Stoll, A. Suárez-Bonnet, B.A. Summers, S.L. Priestnall. Malignant Cutaneous Peripheral Nerve Sheath Tumour with Rhabdomyosarcomatous Differentiation (Triton Tumour) in a Domestic Cat. Journal of Comparative Pathology. 2018; 165 ():1-5.
Chicago/Turabian StyleA.L. Stoll; A. Suárez-Bonnet; B.A. Summers; S.L. Priestnall. 2018. "Malignant Cutaneous Peripheral Nerve Sheath Tumour with Rhabdomyosarcomatous Differentiation (Triton Tumour) in a Domestic Cat." Journal of Comparative Pathology 165, no. : 1-5.
Anaplastic large-cell lymphoma or null-cell lymphoma is a clinical entity reported in people, classified according to the unique appearance of large pleomorphic cells that express CD30. Null-cell lymphoma has also been described in dogs when neither CD3 nor CD79α is expressed by the tumor. We describe a case of lymphoma in the dog in which neoplastic cells did not express routine B- or T-lymphocyte markers on flow cytometry or immunohistochemistry; however, cells immunohistochemically labeled for CD30. The dog in our case died 5 mo after initial presentation, confirming a poor prognosis. Identification of further similar cases in dogs would provide additional prognostic information for this subset of lymphomas. CD30 may also serve as a potential therapeutic target in anaplastic large-cell lymphomas.
Rachel Pittaway; Ying Wu; Balazs Szladovits; Alejandro Suárez-Bonnet; Emma J. Scurrell; Oliver A. Garden; Gerry Polton; Simon Priestnall. Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry. Journal of Veterinary Diagnostic Investigation 2018, 30, 455 -458.
AMA StyleRachel Pittaway, Ying Wu, Balazs Szladovits, Alejandro Suárez-Bonnet, Emma J. Scurrell, Oliver A. Garden, Gerry Polton, Simon Priestnall. Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry. Journal of Veterinary Diagnostic Investigation. 2018; 30 (3):455-458.
Chicago/Turabian StyleRachel Pittaway; Ying Wu; Balazs Szladovits; Alejandro Suárez-Bonnet; Emma J. Scurrell; Oliver A. Garden; Gerry Polton; Simon Priestnall. 2018. "Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry." Journal of Veterinary Diagnostic Investigation 30, no. 3: 455-458.