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Canine gastric carcinoma (CGC) affects both sexes in relatively equal proportions, with a mean age of nine years, and the highest frequency in Staffordshire bull terriers. The most common histological subtype in 149 CGC cases was the undifferentiated carcinoma. CGCs were associated with increased chronic inflammation parameters and a greater chronic inflammatory score when Helicobacter spp. were present. Understanding the molecular pathways of gastric carcinoma is challenging. All markers showed variable expression for each subtype. Expression of the cell cycle regulator 14-3-3σ was positive in undifferentiated, tubular and papillary carcinomas. This demonstrates that 14-3-3σ could serve as an immunohistochemical marker in routine diagnosis and that mucinous, papillary and signet-ring cell (SRC) carcinomas follow a 14-3-3σ independent pathway. p16, another cell cycle regulator, showed increased expression in mucinous and SRC carcinomas. Expression of the adhesion molecules E-cadherin and CD44 appear context-dependent, with switching within tumor emboli potentially playing an important role in tumor cell survival, during invasion and metastasis. Within neoplastic emboli, acinar structures lacked expression of all markers, suggesting an independent molecular pathway that requires further investigation. These findings demonstrate similarities and differences between dogs and humans, albeit further clinicopathological data and molecular analysis are required.
Alexandros Hardas; Alejandro Suárez-Bonnet; Sam Beck; William Becker; Gustavo Ramírez; Simon Priestnall. Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart. Animals 2021, 11, 1409 .
AMA StyleAlexandros Hardas, Alejandro Suárez-Bonnet, Sam Beck, William Becker, Gustavo Ramírez, Simon Priestnall. Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart. Animals. 2021; 11 (5):1409.
Chicago/Turabian StyleAlexandros Hardas; Alejandro Suárez-Bonnet; Sam Beck; William Becker; Gustavo Ramírez; Simon Priestnall. 2021. "Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart." Animals 11, no. 5: 1409.
The different ovine production and breeding systems share the cornerstone of keeping a good body condition to ensure adequate productivity. Several infectious and parasitic disorders have detrimental effects on weight gains and may lead to emaciation. Flock health management procedures are aimed to prevent such conditions. Nutritional management is equally important to guarantee adequate body condition. Persistent bouts of low ruminal pH due to excess concentrate in the diet may lead to subacute ruminal acidosis. Pre-stomach motility disorders may also lead to ill-thrift and emaciation. An adequate mineral supplementation is key to prevent the effects of copper, selenium, and other micronutrients deprivation, which may include, among others, loss of condition. This review elaborates on the clinico-pathologic, diagnostic, and therapeutic aspects of some of these conditions, and highlights the necessity of considering them as contributors to states of wasting in sheep flocks.
Javier Asín; Gustavo A. Ramírez; Mauricio A. Navarro; Akinyi C. Nyaoke; Eileen E. Henderson; Fábio S. Mendonça; Jéssica Molín; Francisco A. Uzal. Nutritional Wasting Disorders in Sheep. Animals 2021, 11, 501 .
AMA StyleJavier Asín, Gustavo A. Ramírez, Mauricio A. Navarro, Akinyi C. Nyaoke, Eileen E. Henderson, Fábio S. Mendonça, Jéssica Molín, Francisco A. Uzal. Nutritional Wasting Disorders in Sheep. Animals. 2021; 11 (2):501.
Chicago/Turabian StyleJavier Asín; Gustavo A. Ramírez; Mauricio A. Navarro; Akinyi C. Nyaoke; Eileen E. Henderson; Fábio S. Mendonça; Jéssica Molín; Francisco A. Uzal. 2021. "Nutritional Wasting Disorders in Sheep." Animals 11, no. 2: 501.
This study describes the clinical and pathological characteristics of cutaneous spindle cell squamous cell carcinoma (SCSCC) in 18 cats. The average age of the cats was 11.8 ± 2.7 years, and all tumors were located in the facial skin, mainly affecting the pinna (13/18, 72%), followed by the periorbital area (4/18, 22%) and the dorsal muzzle (1/18, 6%). Tumors were composed of fusiform neoplastic cells with moderate atypia arranged in solid sheets or fascicles with foci of squamous differentiation. A panel of antibodies against cytokeratins, vimentin, S-100 protein, NSE, GFAP, Melan A, SMA, desmin, CD18, CD31, and p63 was used to help differentiate SCSCC from other spindle cell malignancies. SCSCCs expressed CK5/6 (17/18, 94%), AE1/AE3 (15/18, 83%), and p63 protein (18/18, 100%), but there was no immunolabeling for CK8/18. A role for sunlight exposure in the pathogenesis of the tumors was suggested by changes indicative of actinic keratosis, the location of the tumors in dorsal areas, and the absence of histomorphologic features of papillomavirus infection. Recurrence was not recorded in 14/18 cases (78%) during a follow-up period of 7 to 25 months. Three of 18 (17%) tumors recurred or led to humane euthanasia due to local progression, and one case (5%) had regional lymph node metastasis. Clinical outcome varied with cutaneous location, mitotic count, and invasion of surgical margins; thus, SCSCCs with a more aggressive behavior were located in the periorbital area (4/4 cases), had ≥14 mitoses in 10 high-power fields (2.37 mm2) (4/4 cases), and showed invasion of surgical margins (3/4 cases).
Francisco Rodríguez Guisado; Alejandro Suárez-Bonnet; Gustavo A. Ramírez. Cutaneous Spindle Cell Squamous Cell Carcinoma in Cats: Clinical, Histological, and Immunohistochemical Study. Veterinary Pathology 2021, 58, 503 -507.
AMA StyleFrancisco Rodríguez Guisado, Alejandro Suárez-Bonnet, Gustavo A. Ramírez. Cutaneous Spindle Cell Squamous Cell Carcinoma in Cats: Clinical, Histological, and Immunohistochemical Study. Veterinary Pathology. 2021; 58 (3):503-507.
Chicago/Turabian StyleFrancisco Rodríguez Guisado; Alejandro Suárez-Bonnet; Gustavo A. Ramírez. 2021. "Cutaneous Spindle Cell Squamous Cell Carcinoma in Cats: Clinical, Histological, and Immunohistochemical Study." Veterinary Pathology 58, no. 3: 503-507.
Infectious and parasitic agents have been frequently associated with debilitating and wasting conditions in sheep. The prevalence of these agents has probably undermined the role of toxic causes as contributors to such disorders. In addition, many of these intoxications frequently produce acute clinical disease with specific and characteristic lesions, thus a causal relationship with the toxic substance may be relatively easy to establish. However, persistent exposure to some of these organic or inorganic toxic substances may lead to emaciation, ill-thrift, and poor external aspect. The anti-nutritional factors and alkaloids of several plants, including pyrrolizidine alkaloids, among others, have also been associated with emaciation and/or poor general performance in sheep flocks. In this review, some of these disorders are discussed with an emphasis on clinical signs and lesions, relevant diagnostic aspects, and available therapeutic approaches. In most cases, demonstrating a history of exposure should be one of the most relevant aspects of the diagnostic approach, and removing the animals from the toxic source is the cornerstone of the majority of the treatment strategies.
Jéssica Molín; Fábio Mendonça; Eileen Henderson; Akinyi Nyaoke; Gustavo Ramírez; Mauricio Navarro; Francisco Uzal; Javier Asín. Toxic Wasting Disorders in Sheep. Animals 2021, 11, 229 .
AMA StyleJéssica Molín, Fábio Mendonça, Eileen Henderson, Akinyi Nyaoke, Gustavo Ramírez, Mauricio Navarro, Francisco Uzal, Javier Asín. Toxic Wasting Disorders in Sheep. Animals. 2021; 11 (1):229.
Chicago/Turabian StyleJéssica Molín; Fábio Mendonça; Eileen Henderson; Akinyi Nyaoke; Gustavo Ramírez; Mauricio Navarro; Francisco Uzal; Javier Asín. 2021. "Toxic Wasting Disorders in Sheep." Animals 11, no. 1: 229.
Canine tonsillar polyps are uncommon. We describe 14 tonsillar polyps in dogs and review their classification and pathogenesis. All dogs were adult (3–13 years old). Females (10/14) were more affected than males (4/14). Most of the lesions were asymptomatic (10/14). All lesions were unilateral, pedunculated (9/14), or sessile (5/14), with a smooth (12/14) or papillary/verrucous surface (2/14). Histologically, polyps consisted of benign proliferation of lymphatic vessels, blood vessels, fibrous tissue, and lymphoid tissue in variable proportions, with occasional adipose tissue (4/14). According to the main stromal components, polyps were categorized as lymphangiomatous (5/14), lymphangiolipomatous (2/14), lymphangiofibromatous (2/14), angiofibromatous (1/14), angiofibrolipomatous (1/14), lymphoid (2/14), and myxomatous (1/14). As the pathogenesis of these polyps remains unclear, we propose to replace the term inflammatory tonsillar polyp by a morphological diagnosis based on the stromal characteristics of the lesions. Simple surgical excision was curative in the 9 cases with available follow-up information.
Jéssica Molín; Miquel Vilafranca; Alejandro Suárez-Bonnet; Jaume Altimira; Gustavo A. Ramírez. Canine Tonsillar Polyps: Characteristics, Classification, and Review of the Pathogenesis. Veterinary Pathology 2020, 58, 136 -141.
AMA StyleJéssica Molín, Miquel Vilafranca, Alejandro Suárez-Bonnet, Jaume Altimira, Gustavo A. Ramírez. Canine Tonsillar Polyps: Characteristics, Classification, and Review of the Pathogenesis. Veterinary Pathology. 2020; 58 (1):136-141.
Chicago/Turabian StyleJéssica Molín; Miquel Vilafranca; Alejandro Suárez-Bonnet; Jaume Altimira; Gustavo A. Ramírez. 2020. "Canine Tonsillar Polyps: Characteristics, Classification, and Review of the Pathogenesis." Veterinary Pathology 58, no. 1: 136-141.
We present a unique case of metastatic cholangiocarcinoma with concurrent abdominal cestodiasis in an African green monkey (Chlorocebus pygerythrus) that presented with respiratory insufficiency and abdominal discomfort. There were multiple white–grey masses in the liver and colonic serosa alongside intra-abdominal parasitic cysts. Histopathologically, the liver masses were composed of poorly-differentiated epithelial cells that formed densely cellular solid areas and trabeculae. The neoplastic cells were strongly immunopositive for CK7 but negative for Hep-Par1 antigen, which confirmed a diagnosis of cholangiocarcinoma. Interestingly, there was strong and diffuse neoexpression in the tumour of the cell cycle regulator 14-3-3σ, which is not constitutively expressed in normal liver. There was aberrantly strong expression of E-cadherin, a key cell–cell adhesion protein, in neoplastic cells with evidence of cytoplasmic internalization. This is the first immunohistochemical analysis of 14-3-3σ and E-cadherin in a liver neoplasm in an animal species and the use of these markers requires further investigation in animal liver neoplasms.
A. Suárez-Bonnet; S.L. Priestnall; Gustavo A. Ramirez; J. Molín; J.R. Jaber. Aberrant Expression of Cell Cycle Regulator 14-3-3-σ and E-Cadherin in a Metastatic Cholangiocarcinoma in a Vervet Monkey (Chlorocebus pygerythrus). Journal of Comparative Pathology 2020, 179, 25 -30.
AMA StyleA. Suárez-Bonnet, S.L. Priestnall, Gustavo A. Ramirez, J. Molín, J.R. Jaber. Aberrant Expression of Cell Cycle Regulator 14-3-3-σ and E-Cadherin in a Metastatic Cholangiocarcinoma in a Vervet Monkey (Chlorocebus pygerythrus). Journal of Comparative Pathology. 2020; 179 ():25-30.
Chicago/Turabian StyleA. Suárez-Bonnet; S.L. Priestnall; Gustavo A. Ramirez; J. Molín; J.R. Jaber. 2020. "Aberrant Expression of Cell Cycle Regulator 14-3-3-σ and E-Cadherin in a Metastatic Cholangiocarcinoma in a Vervet Monkey (Chlorocebus pygerythrus)." Journal of Comparative Pathology 179, no. : 25-30.
A. Suárez-Bonnet; S.L. Priestnall; Gustavo A. Ramirez; C. González-Sánchez; J.R. Jaber. Scent (Apocrine) Gland Adenocarcinoma in a Wedge-Capped Capuchin Monkey (Cebus olivaceus): Histological and Immunohistochemical Features. Journal of Comparative Pathology 2020, 179, 1 -6.
AMA StyleA. Suárez-Bonnet, S.L. Priestnall, Gustavo A. Ramirez, C. González-Sánchez, J.R. Jaber. Scent (Apocrine) Gland Adenocarcinoma in a Wedge-Capped Capuchin Monkey (Cebus olivaceus): Histological and Immunohistochemical Features. Journal of Comparative Pathology. 2020; 179 ():1-6.
Chicago/Turabian StyleA. Suárez-Bonnet; S.L. Priestnall; Gustavo A. Ramirez; C. González-Sánchez; J.R. Jaber. 2020. "Scent (Apocrine) Gland Adenocarcinoma in a Wedge-Capped Capuchin Monkey (Cebus olivaceus): Histological and Immunohistochemical Features." Journal of Comparative Pathology 179, no. : 1-6.
Leiomyosarcomas arising from paratesticular structures are rare. We report a case of epididymal sarcoma in an aged male Rottweiler that had histological features suggestive of neural origin but with muscular histogenesis confirmed by immunohistochemistry. Histologically, the lesion comprised a hypercellular neoplasm that had interlacing bundles of spindloid cells, prominent nuclear palisading and many Verocay bodies. Immunohistochemically, the neoplastic cells were strongly positive for smooth muscle markers but negative for neural markers, leading to the diagnosis of leiomyosarcoma. While the presence of nuclear palisading and Verocay bodies has been regarded as strong evidence for the diagnosis of schwannomas, the present report documents the presence of true Verocay bodies in a non-neuronal tumour. It also indicates that immunophenotyping is required for an accurate diagnosis when Verocay bodies are detected in sarcomatous or spindle cell tumours and provides prognostic information on this case. To the best of our knowledge, this is the first report of the presence of true Verocay bodies in a neoplasm other than a nerve sheath tumour in animals.
Gustavo A. Ramirez; A. Suárez-Bonnet; X. Sánchez-Salguero; J. Molín. Primary Verocay Body-rich Epididymal Leiomyosarcoma in a Dog. Journal of Comparative Pathology 2020, 178, 27 -31.
AMA StyleGustavo A. Ramirez, A. Suárez-Bonnet, X. Sánchez-Salguero, J. Molín. Primary Verocay Body-rich Epididymal Leiomyosarcoma in a Dog. Journal of Comparative Pathology. 2020; 178 ():27-31.
Chicago/Turabian StyleGustavo A. Ramirez; A. Suárez-Bonnet; X. Sánchez-Salguero; J. Molín. 2020. "Primary Verocay Body-rich Epididymal Leiomyosarcoma in a Dog." Journal of Comparative Pathology 178, no. : 27-31.
Proliferative disorders of lymphatic origin in animals are mostly congenital or occur within the first few months of life. Involvement of internal organs is extremely infrequent. A seven-year-old entire female mixed-breed dog was presented with apathy and poor appetite. Abdominal ultrasonography revealed a focally enlarged spleen with an anechoic round lesion. Splenectomy was performed and pathological examination demonstrated a sponge-like, compressible tumour composed of endothelium-lined vascular cystic spaces filled with eosinophilic proteinaceous material lacking erythrocytes. Immunohistochemical stains showed that cyst-lining cells were strongly positive for CD31 and factor VIII and focally positive for lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1). Based on these findings, the lesion was identified as splenic cystic lymphangioma. To the best of our knowledge, this is the first description of solitary lymphangioma of the spleen in animals.
G.A. Ramírez; X. Sánchez-Salguero; J. Molín. Primary Cystic Lymphangioma of the Spleen in an Adult Dog. Journal of Comparative Pathology 2020, 178, 22 -26.
AMA StyleG.A. Ramírez, X. Sánchez-Salguero, J. Molín. Primary Cystic Lymphangioma of the Spleen in an Adult Dog. Journal of Comparative Pathology. 2020; 178 ():22-26.
Chicago/Turabian StyleG.A. Ramírez; X. Sánchez-Salguero; J. Molín. 2020. "Primary Cystic Lymphangioma of the Spleen in an Adult Dog." Journal of Comparative Pathology 178, no. : 22-26.
In this retrospective study, we describe the histopathological findings in seven papillomas and 45 squamous cell carcinomas (SCCs) from psittacine birds, raptors and domestic fowl. The age of affected birds ranged from 3 to 40 years, with median age significantly higher in psittacines (P = 0.014). The majority of tumours were located in the skin (24/52, 46.2%) or uropygial gland (10/52, 19.2%). Thirty of the SCCs (66.7%) were well differentiated and 15 (33.3%) were poorly-differentiated. SCCs exhibited a significantly higher degree of nuclear pleomorphism (P = 0.005) and a greater proportion were ulcerated (P = 0.001) compared with papillomas; however, there was no significant difference in mitotic count (MC) or inflammation score. The expression of cyclo-oxygenase (COX)-2 and E-cadherin was investigated by immunohistochemistry. The COX-2 total score (TS) was significantly higher in SCCs compared with papillomas (P = 0.002), but the difference between COX-2 TS of well- and poorly-differentiated SCCs was not significant. COX-2 labelling was predominantly cytoplasmic, but some tumours had concurrent membranous and/or perinuclear labelling. SCCs with membranous labelling had a significantly higher MC (P = 0.028). A significantly higher proportion of SCCs were negative for E-cadherin compared with papillomas (P = 0.042), but there was no significant difference between well- and poorly-differentiated SCCs. Fourteen papillomas and SCCs from psittacines were also tested by polymerase chain reaction for the presence of Psittacus erithacus papillomavirus 1 and Psittacid herpesvirus 1, but all samples tested negative. We demonstrate for the first time the expression of COX-2 and E-cadherin in avian tissues, and suggest that these markers may be useful in differentiating papillomas from SCCs, particularly when sample size is small.
A.L. Jones; A. Suárez-Bonnet; J.A. Mitchell; Gustavo A. Ramirez; M.F. Stidworthy; S.L. Priestnall. Avian Papilloma and Squamous Cell Carcinoma: a Histopathological, Immunohistochemical and Virological study. Journal of Comparative Pathology 2020, 175, 13 -23.
AMA StyleA.L. Jones, A. Suárez-Bonnet, J.A. Mitchell, Gustavo A. Ramirez, M.F. Stidworthy, S.L. Priestnall. Avian Papilloma and Squamous Cell Carcinoma: a Histopathological, Immunohistochemical and Virological study. Journal of Comparative Pathology. 2020; 175 ():13-23.
Chicago/Turabian StyleA.L. Jones; A. Suárez-Bonnet; J.A. Mitchell; Gustavo A. Ramirez; M.F. Stidworthy; S.L. Priestnall. 2020. "Avian Papilloma and Squamous Cell Carcinoma: a Histopathological, Immunohistochemical and Virological study." Journal of Comparative Pathology 175, no. : 13-23.
J. Molín; M. Vilafranca; A. Suárez-Bonnet; J. Altimira; Gustavo A. Ramirez. Adenolipomas in Dogs: Expanding the Spectrum of Canine Lipomas and of Glandular Components in Adenolipomas. Journal of Comparative Pathology 2020, 174, 181 .
AMA StyleJ. Molín, M. Vilafranca, A. Suárez-Bonnet, J. Altimira, Gustavo A. Ramirez. Adenolipomas in Dogs: Expanding the Spectrum of Canine Lipomas and of Glandular Components in Adenolipomas. Journal of Comparative Pathology. 2020; 174 ():181.
Chicago/Turabian StyleJ. Molín; M. Vilafranca; A. Suárez-Bonnet; J. Altimira; Gustavo A. Ramirez. 2020. "Adenolipomas in Dogs: Expanding the Spectrum of Canine Lipomas and of Glandular Components in Adenolipomas." Journal of Comparative Pathology 174, no. : 181.
Paraneoplastic pemphigus is an autoimmune blistering skin disease associated with concurrent neoplasia that is rarely observed in veterinary medicine. This case report presents a ten-year-old female Labrador Retriever dog with generalized seborrhea and alopecic, crusting and ulcerative lesions in the periocular and perioral regions, the lips and the groins. Hematology analysis showed a marked increase in the number of lymphocytes, while the rest of the values were normal. Skin biopsies were collected and the histological findings were consistent with pemphigus in association with an epitheliotropic lymphoma. Immunohistochemical analysis was performed. There was deposition of immunoglobulin G (IgG) in the basement membrane and in the intercellular space that corresponded with an immunostaining pattern characteristic of pemphigus erythematosus (PE). The lymphoma was positive to CD3 cells and was classified as a T-cell epitheliotropic lymphoma. To our knowledge, this is the first case of PE and cutaneous lymphoma coexisting in a dog, expanding the list of associations between immune-mediated diseases and cancer in dogs, and providing support to the eventual connection between autoimmunity and neoplasia in this species.
A. Suárez-Bonnet; B. García-González; Gustavo A. Ramirez; C. Peñafiel-Verdú; J. R. Jaber. Pemphigus erythematosus and cutaneous epitheliotropic lymphoma in a Labrador Retriever dog. 2019, 20, 221 -224.
AMA StyleA. Suárez-Bonnet, B. García-González, Gustavo A. Ramirez, C. Peñafiel-Verdú, J. R. Jaber. Pemphigus erythematosus and cutaneous epitheliotropic lymphoma in a Labrador Retriever dog. . 2019; 20 (3):221-224.
Chicago/Turabian StyleA. Suárez-Bonnet; B. García-González; Gustavo A. Ramirez; C. Peñafiel-Verdú; J. R. Jaber. 2019. "Pemphigus erythematosus and cutaneous epitheliotropic lymphoma in a Labrador Retriever dog." 20, no. 3: 221-224.
Merkel cells (MCs) are neuroendocrine cells involved with tactile sense, growth, differentiation and homeostasis of the skin as well as in different cutaneous diseases. Specific staining techniques are required for their identification because they are not easily visible in paraffin sections stained with hematoxylin and eosin. The present study assess the histochemical features of the MCs in dogs comparing with those described for other mammals in the literature and with the use of immunohistochemistry. A systematic study of samples from MCs‐rich areas from healthy dogs was carried out by use of several histologic stains, including metachromatic staining, silver stains, methylene blue, periodic acid–Schiff stain, and osmium‐based staining method. MCs were detected by the Grimelius argyrophilic stain in 86.7% of the specimens. The staining was showed as dark‐brown granular cytoplasmic and consistently polarized to the basal cell cytoplasm matching with the cellular distribution of the characteristic neurosecretory granules. Some modifications in the standard staining protocol, including rinsing, silver reimpregnation, and counterstain dye, enhanced the MCs identification in stratified squamous epithelium. When compared with Cytokeratin 20‐immunolabeled serial sections several MCs appeared non‐stained with the argyrophilic method. These differences in MC numbers between stains were statistically significant. Other histologic stains failed to identify MCs in the specimens. The results of this study indicate that Grimelius argyrophilic stain is a suitable method for demonstration of MCs in the stratified squamous epithelium of skin and mucosa. Discussion on its utility when compared with immunohistochemistry and a review of the scientific literature is also presented. This article is protected by copyright. All rights reserved.
Gustavo A. Ramírez; Antonio Espinosa De Los Monteros. Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs. The Anatomical Record 2018, 302, 1458 -1464.
AMA StyleGustavo A. Ramírez, Antonio Espinosa De Los Monteros. Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs. The Anatomical Record. 2018; 302 (8):1458-1464.
Chicago/Turabian StyleGustavo A. Ramírez; Antonio Espinosa De Los Monteros. 2018. "Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs." The Anatomical Record 302, no. 8: 1458-1464.
A 2-year-old entire female mixed-breed dog was presented with signs of cholestasis. Abdominal ultrasonography revealed a gallbladder with a thickened and hyperechoic wall and luminal calculi. Exploratory laparotomy with cholecystectomy was performed and histopathological examination of the specimens from the cholecystectomy demonstrated extensive proliferation of large, prominent nerves containing ganglion cells with no atypia mainly located in the gallbladder mucosa. The neural nature of these components was confirmed by immunohistochemical labelling with antibodies specific for synaptophysin, glial fibrillary acidic protein, S100 protein and neurofilaments. Based on these findings, the lesion was diagnosed as ganglioneuromatosis. Reports of ganglioneuromatosis in animals have so far been restricted to the intestine. This is the first case of ganglioneuromatosis affecting the gallbladder in an animal in which no intestinal involvement was apparent.
G.A. Ramírez; J. Altimira; M Vilafranca. Ganglioneuromatosis of the Gallbladder in a Dog with Cholecystitis and Cholestasis. Journal of Comparative Pathology 2018, 163, 29 -32.
AMA StyleG.A. Ramírez, J. Altimira, M Vilafranca. Ganglioneuromatosis of the Gallbladder in a Dog with Cholecystitis and Cholestasis. Journal of Comparative Pathology. 2018; 163 ():29-32.
Chicago/Turabian StyleG.A. Ramírez; J. Altimira; M Vilafranca. 2018. "Ganglioneuromatosis of the Gallbladder in a Dog with Cholecystitis and Cholestasis." Journal of Comparative Pathology 163, no. : 29-32.
A one-year-old intact male German shepherd dog was referred with a 3-month history of dysuria and pollakiuria. Physical examination revealed a large firm mass in the caudal abdomen. Findings from survey radiography, negative contrast cystography, computed tomographic (CT) retrograde positive contrast cystography, and CT excretory urography were consistent with a large urinary bladder diverticulum. An exploratory laparotomy revealed a normal wall appearance in the ventral compartment (true bladder) and marked thinning of the wall in the dorsal compartment (diverticulum). Both ureters inserted into the ventral compartment. The dorsal compartment was excised and histopathology confirmed the diagnosis of urinary bladder diverticulum.
Agustina Ansón; Carina Strohmayer; Jose M. Larrinaga; Esteban Iglesias; Ramón Almela; Gustavo Ramirez; Vicente Cervera. Computed tomographic retrograde positive contrast cystography and computed tomographic excretory urography characterization of a urinary bladder diverticulum in a dog. Veterinary Radiology & Ultrasound 2018, 60, 1 .
AMA StyleAgustina Ansón, Carina Strohmayer, Jose M. Larrinaga, Esteban Iglesias, Ramón Almela, Gustavo Ramirez, Vicente Cervera. Computed tomographic retrograde positive contrast cystography and computed tomographic excretory urography characterization of a urinary bladder diverticulum in a dog. Veterinary Radiology & Ultrasound. 2018; 60 (6):1.
Chicago/Turabian StyleAgustina Ansón; Carina Strohmayer; Jose M. Larrinaga; Esteban Iglesias; Ramón Almela; Gustavo Ramirez; Vicente Cervera. 2018. "Computed tomographic retrograde positive contrast cystography and computed tomographic excretory urography characterization of a urinary bladder diverticulum in a dog." Veterinary Radiology & Ultrasound 60, no. 6: 1.
Gustavo A. Ramírez; Francisco Rodríguez; Alejandro Suárez-Bonnet; Pedro Herráez; Ayoze Castro-Alonso; Miguel Rivero; Antonio Espinosa De Los Monteros. Study of Merkel cells in the dog through the immunohistochemical expression of five different commercial antibodies: comparative analysis. Journal of Applied Animal Research 2017, 46, 417 -421.
AMA StyleGustavo A. Ramírez, Francisco Rodríguez, Alejandro Suárez-Bonnet, Pedro Herráez, Ayoze Castro-Alonso, Miguel Rivero, Antonio Espinosa De Los Monteros. Study of Merkel cells in the dog through the immunohistochemical expression of five different commercial antibodies: comparative analysis. Journal of Applied Animal Research. 2017; 46 (1):417-421.
Chicago/Turabian StyleGustavo A. Ramírez; Francisco Rodríguez; Alejandro Suárez-Bonnet; Pedro Herráez; Ayoze Castro-Alonso; Miguel Rivero; Antonio Espinosa De Los Monteros. 2017. "Study of Merkel cells in the dog through the immunohistochemical expression of five different commercial antibodies: comparative analysis." Journal of Applied Animal Research 46, no. 1: 417-421.
A 13-year-old male cat presented with an ill-defined mass in the rostral mandible causing destruction and loss of alveolar bone. Microscopically, the mass consisted of cords or islands of benign odontogenic epithelium and a malignant, pleomorphic spindle-shaped cell component with dysplastic dentine formation. Immunohistochemically, neoplastic mesenchymal cells proved to be strongly positive for vimentin and negative for cytokeratins, desmin, actin and S100 protein; the Ki67 proliferation index was high. Morphological and immunohistochemical features largely overlap those reported for ameloblastic fibrodentinosarcoma, an uncommon histologic subtype of odontogenic sarcoma recognised in humans but no reported previously in animals. Ki-67 expression assessment may help to discriminate between malignant and benign forms of odontogenic tumours but the final diagnosis is mainly morphological.
Gustavo A. Ramírez; Lorenzo Ressel; Jaume Altimira; Miquel Vilafranca. Mandibular odontogenic sarcoma (ameloblastic fibrodentinosarcoma) in an aged cat — Short communication. Acta Veterinaria Hungarica 2017, 65, 89 -95.
AMA StyleGustavo A. Ramírez, Lorenzo Ressel, Jaume Altimira, Miquel Vilafranca. Mandibular odontogenic sarcoma (ameloblastic fibrodentinosarcoma) in an aged cat — Short communication. Acta Veterinaria Hungarica. 2017; 65 (1):89-95.
Chicago/Turabian StyleGustavo A. Ramírez; Lorenzo Ressel; Jaume Altimira; Miquel Vilafranca. 2017. "Mandibular odontogenic sarcoma (ameloblastic fibrodentinosarcoma) in an aged cat — Short communication." Acta Veterinaria Hungarica 65, no. 1: 89-95.
L. Ressel; R. Finotello; R. Blundell; M.E. Turba; J. Altimira; Gustavo A. Ramirez; M. Vilafranca. Atypical Phenotypes in Canine Lymphomas: The More You Search the More You Find. Journal of Comparative Pathology 2017, 156, 61 .
AMA StyleL. Ressel, R. Finotello, R. Blundell, M.E. Turba, J. Altimira, Gustavo A. Ramirez, M. Vilafranca. Atypical Phenotypes in Canine Lymphomas: The More You Search the More You Find. Journal of Comparative Pathology. 2017; 156 (1):61.
Chicago/Turabian StyleL. Ressel; R. Finotello; R. Blundell; M.E. Turba; J. Altimira; Gustavo A. Ramirez; M. Vilafranca. 2017. "Atypical Phenotypes in Canine Lymphomas: The More You Search the More You Find." Journal of Comparative Pathology 156, no. 1: 61.
Canine melanoma (CMM) more commonly affects the oral mucosa and the cutis. CMM shares several features with human melanomas (HMM), included resistance to a broad variety of antineoplastic chemotherapy agents. P-glycoprotein 1 (Pgp) expression is a well-recognized feature of multi-drug resistance and the purpose of this study was to investigate its expression in treatment naïve CMM. We also investigated Pgp association with tumour location and histological features. Histology records of CMM were retrieved, including patients from 2012–2014. Twenty-five cases of CMM were included in this study. Results revealed that Pgp is expressed in CMM and oral tumours were more likely to have a membranous Pgp expression (100%) than cutaneous tumours (66.6%) (P = 0.010). Cytoplasmic and nuclear Pgp expression could also be identified. Results of this study bring useful data that help in understanding one of the possible mechanisms responsible of intrinsic chemotherapy resistance in canine CMM.
Riccardo Finotello; J. M. Monné Rodriguez; M. Vilafranca; J. Altimira; G. A. Ramirez; A. Haines; Lorenzo Ressel. Immunohistochemical expression of MDR1-Pgp 170 in canine cutaneous and oral melanomas: pattern of expression and association with tumour location and phenotype. Veterinary and Comparative Oncology 2016, 15, 1393 -1402.
AMA StyleRiccardo Finotello, J. M. Monné Rodriguez, M. Vilafranca, J. Altimira, G. A. Ramirez, A. Haines, Lorenzo Ressel. Immunohistochemical expression of MDR1-Pgp 170 in canine cutaneous and oral melanomas: pattern of expression and association with tumour location and phenotype. Veterinary and Comparative Oncology. 2016; 15 (4):1393-1402.
Chicago/Turabian StyleRiccardo Finotello; J. M. Monné Rodriguez; M. Vilafranca; J. Altimira; G. A. Ramirez; A. Haines; Lorenzo Ressel. 2016. "Immunohistochemical expression of MDR1-Pgp 170 in canine cutaneous and oral melanomas: pattern of expression and association with tumour location and phenotype." Veterinary and Comparative Oncology 15, no. 4: 1393-1402.
Merkel cells (MCs) are specialized cutaneous receptor cells involved with tactile sense. Although the distribution of MCs has been extensively studied in humans and rodents, their precise distribution and density throughout skin in the dog has not previously been determined. Knowledge of their distribution could facilitate understanding of their functions. By using of immunohistochemistry, density and anatomical mapping of the MCs population in the dog skin was determined. Assessment of the MCs innervation was also achieved. Different patterns were noted in epidermis, hair follicles or mucosa, including variable-sized clusters, linear or horse-shaped arrangements, and scattered and individualized cells. MCs revealed great variations in density and distribution over the body surface, with the highest numbers in oral mucosa and facial skin. There was no correlation of MCs density with age, sex, type of breed, coat type or pigmentation. Between 41-65% of MCs in hairy and glabrous skin and 8-18% of MCs in oral mucosa were in intimate contact with intraepithelial axon terminals. These findings indicate that canine MCs are numerous in sensory receptive areas and may be associated with the tactile sense in the dog. The present paper enhances the knowledge of the skin structure in this species. This article is protected by copyright. All rights reserved.
Gustavo A. Ramírez; Francisco Rodríguez; Óscar Quesada; Pedro Herráez; Antonio Fernández; Antonio Espinosa-De-Los-Monteros. Anatomical Mapping and Density of Merkel Cells in Skin and Mucosae of the Dog. The Anatomical Record 2016, 299, 1157 -1164.
AMA StyleGustavo A. Ramírez, Francisco Rodríguez, Óscar Quesada, Pedro Herráez, Antonio Fernández, Antonio Espinosa-De-Los-Monteros. Anatomical Mapping and Density of Merkel Cells in Skin and Mucosae of the Dog. The Anatomical Record. 2016; 299 (9):1157-1164.
Chicago/Turabian StyleGustavo A. Ramírez; Francisco Rodríguez; Óscar Quesada; Pedro Herráez; Antonio Fernández; Antonio Espinosa-De-Los-Monteros. 2016. "Anatomical Mapping and Density of Merkel Cells in Skin and Mucosae of the Dog." The Anatomical Record 299, no. 9: 1157-1164.