The extraction and characterization of defined and carboxyl-rich oligomeric lignin fragments with narrow molecular weight distribution is presented herein. With regard to the well-known pulp bleaching process, oxidative lignin depolymerization was investigated using hydrogen peroxide in an aqueous alkaline solution (i.e., at T = 318 K, t = 1 h) and subsequent selective fractionation with a 10/90 (v/v) acetone/water mixture. While the weight average molecular weight (M_W) of lignin in comparison to the starting material was reduced by 82% after oxidation (T = 318 K, t = 1 h, c_lignin = 40 g L⁻¹, c_H2O2 = 80 g L⁻¹, c_NaOH = 2 mol L⁻¹) and subsequent solvent fractionation (T = 298 K, t = 18 h, c_{cleavage product} = 20 g L⁻¹), the carboxyl group (–COOH) content increased from 1.29 mmol g⁻¹ up to 2.66 mmol g⁻¹. Finally, the successful scale-up of this whole process to 3 L scale led to gram amounts (14% yield) of oligomeric lignin fragments with a M_W of 1607 g mol⁻¹, a number average molecular weight (M_N) of 646 g mol⁻¹, a narrow polydispersity index of 3.0, and a high –COOH content of 2.96 mmol g⁻¹. Application of these oligomeric lignin fragments in epoxy resins or as adsorbents is conceivable without further functionalization.

This work evaluates the direct etherification of bio-sourced isobutylene with ethanol forming fully renewable ethyl tert-butyl ether (ETBE). Challenge was the use of the fermentative produced substrate in a solvent-free catalysis using the acidic ion exchanger Amberlyst-15. CO₂ impurities have a significant influence on the liquid phase process. The conversion at a ratio that is based on molarities of isobutylene to ethanol of 1:0.9 results in yields up to 97 mol% ETBE. Purities up to 99 wt.% could be achieved by subsequent water extraction in order to ensure qualities for technical application as high performance fuel additive.