In this paper, an all-solid-state nitrate doped polypyrrole (PPy(NO₃⁻) ion-selective electrode (ISE) was prepared with a nanohybrid composite film of gold nanoparticles (AuNPs) and electrochemically reduced graphene oxide (ERGO). Preliminary tests on the ISE based in-situ soil nitrate–nitrogen (NO₃⁻-N) monitoring was conducted in a laboratory 3-stage column. Comparisons were made between the NO₃⁻-N content of in-situ soil percolate solution and laboratory-prepared extract solution. Possible influential factors of sample depth, NO₃⁻-N content, soil texture, and moisture were varied. Field-emission scanning electron microscopy (FESEM) and X-ray powder diffraction (XRD) characterized morphology and content information of the composite film of ERGO/AuNPs. Due to the performance excellence for conductivity, stability, and hydrophobicity, the ISE with ERGO/AuNPs illustrates an acceptable detection range from 10⁻¹ to 10⁻⁵ M. The response time was determined to be about 10 s. The lifetime was 65 days, which revealed great potential for the implementation of the ERGO/AuNPs mediated ISE for in-situ NO₃⁻-N monitoring. In-situ NO₃⁻-N testing results conducted by the all-solid-state ISE followed a similar trend with the standard UV-VIS method.

Salmonella is a main cause of foodborne illnesses and rapid screening of Salmonella is the key to prevent Salmonella outbreaks, however available detection methods either require a long time, or need complex pretreatment, or have low sensitivity. In this study, a microfluidic biosensor was developed for Salmonella detection using viscoelastic inertial microfluidics for separating magnetic bacteria from unbound magnetic nanoparticles (MNPs) and enzyme catalytic colorimetry for amplifying biological signals. The polyclonal antibodies and horseradish peroxidase (HRP) modified MNPs were first used to specifically capture Salmonella to form magnetic HRP-bacteria. Both magnetic HRP-bacteria and unbound MNPs were magnetically separated from background and resuspended in viscoelastic polyvinylpyrrolidone solution as sample flow. When sample flow was injected with polyvinylpyrrolidone sheath flow into a T-shaped microchannel, larger-sized magnetic HRP-bacteria could penetrate the sample flow, however smaller-sized MNPs remained in the sample flow due to weaker inertial lift force and elastic lift force, resulting in continuous-flow separation of magnetic HRP-bacteria. Finally, magnetic HRP-bacteria were collected and concentrated to catalyze tetramethyl benzidine, and absorbance was measured to determine the bacteria. This biosensor was able to detect Salmonella as low as 30 CFU/mL in 1 h and featured the advantages of shorter time due to a one-step immunoreaction, easier extension due to only one antibody and one label, and lower cost due to less expensive materials.