The diet of juvenile bluefin tuna (Thunnus thynnus) caught 2 to 8 miles off NE Sicily was investigated in order to improve knowledge of the species’ early life history. From 1998 to 2000, 107 specimens ranging from 63 to 495 mm (total length) were fished between July and November. Fishes were caught by trolling line or purse seine in a Fish Aggregating Devices (FADs) area 2 to 8 miles offshore and as by-catch of the purse-seine sardine fishery 2 to 4 miles offshore. Most frequently found items in the T. thynnus stomachs were fishes (84.5%), crustaceans (54.6%) and cephalopods (50.5%). The largest contribution in weight was provided by cephalopods (47.3%) and fishes (46.5%), while the most abundant items were fishes (51%), cephalopods (27.2%) and crustaceans (21.1%). These results suggest that young-of-the-year tuna have an essentially piscivorous diet, although invertebrate prey provide a substantial contribution to the food array. Prey show little relationship with FADs, although one prey species (blue runner, Caranx crysos) is associated with FADs in the Mediterranean.