The human papillomavirus (HPV) directly infects cervical keratinocytes and interferes with TLR signalling. To shed light on the effect of HPV on upstream receptors, we evaluated TLRs 1–9 gene expression in HPV-negative normal and HPV-positive pre-malignant and malignantex vivocervical tissue. Quantitative real-time polymerase chain reaction was performed separately for epithelial and stromal tissue compartments. Differences in gene expression were analyzed by the Jonckheere-Terpstra trend test or the Student'st-test for pairwise comparison. Laser capture microdissection revealed an increase in TLR3 and a decrease in TLR1 mRNA levels in dysplastic and carcinoma epithelium, respectively. In the stroma, a trend of increasing TLR 1, 2, 5, 6, and 9 mRNA levels with disease severity was found. These findings implicate the involvement of TLR3 and TLR1 in early and late cervical carcinogenesis, respectively, suggesting that stromal upregulation of TLRs may play a role in cervical disease progression.

Whey protein supplementation (WPS) has been shown to improve functional outcomes in populations that are able to participate in high-intensity resistance training. The purpose of the study was to evaluate the efficacy of WPS on rehabilitation outcomes in a frail, hospitalized elderly population. Men and women (n = 47) were randomly assigned to either a control group or WPS group for the length of their hospital stay. Several functional and serum measures were determined pre- and post-intervention. WPS significantly increased average daily protein intake and was well tolerated. The WPS group exhibited significant improvements in grip strength and knee extensor force over the control group, and a significant positive correlation was found between change in prealbumin and percent-increase knee extensor force. These findings support the use of WPS to improve protein nutritional status and rehabilitation outcomes in a clinical setting involving a frail, elderly population.

Primary objective: Persistent concussion symptoms (PCS) affect 10–30% of individuals after sports-related concussion. This study evaluated the effect of exercise-based rehabilitation on symptom scores, brain-derived neurotrophic factor (BDNF), cognitive functions and static balance in a sample of participants with PCS. Research design: One group pre-test post-test pilot study. Methods and procedure: Nine participants with PCS received a structured exercise-based rehabilitation program. Changes in symptom scores, BDNF, cognitive functions and measures of static balance were used to evaluate the utility of the exercise program. Main outcome and results: The results of this pilot study indicate a significant improvement in symptom scores following treatment, as well as some associated benefits in regards to cognitive function and static balance. BDNF levels in the participants with PCS within this study are notably lower than in a previous study on healthy controls. Conclusions: The preliminary evidence reported in the current pilot study is clinically relevant as our findings suggest exercise-based treatments may improve PCS outcomes in a more favourable manner than rest-based treatment.

Ionizing radiation (IR) is known to cause fetal programming, but the physiological effects of low-dose IR are not fully understood. This study examined the effect of low (50 mGy) to non-lethal (300 and 1000 mGy) radiation exposure during late gestation on cardiac metabolism and oxidative stress in adult offspring. Pregnant C57BL/6J mice were exposed to 50, 300, or 1000 mGy of gamma radiation or Sham irradiation on gestational day 15. Sixteen weeks after birth, ¹⁸F-Fluorodeoxyglucose (FDG) uptake was examined in the offspring using Positron Emission Tomography imaging. Western blot was used to determine changes in oxidative stress, antioxidants, and insulin signaling related proteins. Male and female offspring from irradiated dams had lower body weights when compared to the Sham. 1000 mGy female offspring demonstrated a significant increase in ¹⁸F-FDG uptake, glycogen content, and oxidative stress. 300 and 1000 mGy female mice exhibited increased superoxide dismutase activity, decreased glutathione peroxidase activity, and decreased reduced/oxidized glutathione ratio. We conclude that non-lethal radiation during late gestation can alter glucose uptake and increase oxidative stress in female offspring. These data provide evidence that low doses of IR during the third trimester are not harmful but higher, non-lethal doses can alter cardiac metabolism later in life and sex may have a role in fetal programming.

Selective androgen receptor modulators (SARMs) are important hypertrophic molecules that are potential treatments for many types of myopathy and osteopathy. This study aimed to determine if the SARM RAD140 had additive effects on muscle hypertrophy when combined with the functional overloading (FO) of the plantaris muscle. Male, Sprague–Dawley rats (n = 10 rats/group) were randomly selected into one of four treatment groups: (1) RAD140‐FO, (2) RAD140‐Control, (3) Vehicle‐Control, or (4) Vehicle‐FO. RAD140 groups received drugs through drinking water, and control groups received only Vehicle (methylcellulose). Standard rat chow and water were provided ad libitum. Muscle weights of the triceps‐surae group and muscle fiber cross‐sectional area (CSA) were measured. Muscle weight analysis showed a marked increase in RAD140‐FO groups but was not statistically different from the Vehicle‐FO group. CSA results indicated similar findings; however, RAD140‐Control showed significantly elevated CSA compared to Vehicle‐Control. The tibial microarchitecture was analyzed using micro computed tomography. RAD140 did not impact cortical or trabecular bone structural morphometric properties following 14 days of treatment. The data presented here show the potential of RAD140 to stimulate muscle hypertrophy in young healthy rats.

Significant depots of brown adipose tissue (BAT) have been identified in many adult humans through positron emission tomography (PET), with the amount of BAT being inversely correlated with obesity. As dietary activation of BAT has implications for whole body glucose metabolism, leucine was used in the present study to determine its ability to promote BAT activation resulting in increased glucose uptake. In order to assess this, 2-deoxy-2-(fluorine-18)fluoro-d-glucose (¹⁸F-FDG) uptake was measured in C57BL/6 mice using microPET after treatment with leucine, glucose, or both in interscapular BAT (IBAT). Pretreatment with propranolol (PRP) was used to determine the role of β-adrenergic activation in glucose and leucine-mediated ¹⁸F-FDG uptake. Analysis of maximum standardized uptake values (SUV_MAX) determined that glucose administration increased ¹⁸F-FDG uptake in IBAT by 25.3%. While leucine did not promote ¹⁸F-FDG uptake alone, it did potentiate glucose-mediated ¹⁸F-FDG uptake, increasing ¹⁸F-FDG uptake in IBAT by 22.5%, compared to glucose alone. Pretreatment with PRP prevented the increase in IBAT ¹⁸F-FDG uptake following the combination of glucose and leucine administration. These data suggest that leucine is effective in promoting BAT ¹⁸F-FDG uptake through β-adrenergic activation in combination with glucose.