Hybrids of larch (Larix kaempferi × Larix olgensis) are important afforestation species in northeastern China. They are routinely propagated via rooted stem cuttings. Despite the importance of rooting, little is known about the regulation of adventitious root development in larch hybrids. 454 GS FLX Titanium technology represents a new method for characterizing the transcriptomes of non-model species. This method can be used to identify differentially expressed genes, and then two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analyses can be used to analyze their corresponding proteins. In this study, we analyzed semi-lignified cuttings of two clones of L. kaempferi × L. olgensis with different rooting capacities to study the molecular basis of adventitious root development. We analyzed two clones; clone 25-5, with strong rooting capacity, and clone 23-12, with weak rooting capacity. We constructed four cDNA libraries from 25-5 and 23-12 at two development stages. Sequencing was conducted using the 454 pyrosequencing platform. A total of 957832 raw reads was produced; 95.07% were high-quality reads, and were assembled into 45137 contigs and 61647 singletons. The functions of the unigenes, as indicated by their Gene Ontology annotation, included diverse roles in the molecular functions, biological processes, and cellular component categories. We analyzed 75 protein spots (-fold change ≥2, P ≤0.05) by 2D-DIGE, and identified the differentially expressed proteins using MALDI-TOF/TOF MS. A joint analysis of transcriptome and proteome showed genes related to two pathways, polyamine synthesis and stress response, might play an important role on adventitious root development. These results provide fundamental and important information for research on the molecular mechanism of adventitious root development. We also demonstrated for the first time the combined use of two important technologies as a powerful approach to advance research on non-model, but otherwise important, larch species.

With the development of national-scale forest biomass monitoring work, accurate estimation of forest biomass on a large scale is becoming an important research topic in forestry. In this study, the stem wood, branches, stem bark, needles, roots and total biomass models for larch were developed at the regional level, using a general allometric equation, a dummy variable model, a mixed effects model, and a Bayesian hierarchical model, to select the most effective method for predicting large-scale forest biomass. Results showed total biomass of trees with the same diameter gradually decreased from southern to northern regions in China, except in the Hebei province. We found that the stem wood, branch, stem bark, needle, root, and total biomass model relationships were statistically significant (p-values < 0.01) for the general allometric equation, linear mixed model, dummy variable model, and Bayesian hierarchical model, but the linear mixed, dummy variable, and Bayesian hierarchical models showed better performance than the general allometric equation. An F-test also showed significant differences between the models. The R² average values of the linear mixed model, dummy variable model, and Bayesian hierarchical model were higher than those of the general allometric equation by 0.007, 0.018, 0.015, 0.004, 0.09, and 0.117 for the total tree, root, stem wood, stem bark, branch, and needle models respectively. However, there were no significant differences between the linear mixed model, dummy variable model, and Bayesian hierarchical model. When the number of categories was increased, the linear mixed model and Bayesian hierarchical model were more flexible and applicable than the dummy variable model for the construction of regional biomass models.

Taxus chinensis is a precious woody species with significant economic value. Anthocyanin as flavonoid derivatives plays a crucial role in plant biology and human health. However, the genes involved in anthocyanin biosynthesis have not been identified in T. chinensis. In this study, twenty-five genes involved in anthocyanin biosynthesis were identified, including chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, anthocyanidin synthase, flavonoid 3’-hydroxylase, flavonoid 3’,5’-hydroxylase, dihydroflavonol 4-reductase, anthocyanidin reductase, and leucoanthocyanidin reductase. The conserved domains and phylogenetic relationships of these genes were characterized. The expression levels of these genes in different tissues and different ages of xylem were investigated. Additionally, the anthocyanin accumulation in xylem of different ages of T. chinensis was measured. The results showed the anthocyanin accumulation was correlated with the expression levels of dihydroflavonol 4-reductase, anthocyanidin synthase, flavonoid 3’-hydroxylase, and flavonoid 3’,5’-hydroxylase. Our results provide a basis for studying the regulation of the biosynthetic pathway for anthocyanins and wood color formation in T. chinensis.