Here, we report a novel enantioselective electrochemical biosensor for the discrimination of cystine enantiomers (D- and L-cystine) using a chiral interface for the specific recognition of D- and L-cystine. The biosensor is based on reduced graphene oxide modified by β-cyclodextrin (rGO/β-CD) at the GCE surface. During the preparation of rGO/β-CD/GCE, the modified electrode surfaces were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). The electrochemical behaviours of the D- and L-cystine were investigated using the rGO/β-CD/GCE by CV and compared to bare GCE. A clear separation between the oxidation peak potentials of D- and L-cystine was observed at 1.32 and 1.42 V, respectively. The electrochemical discrimination performance of the fabricated chiral sensor was also examined by differential pulse voltammetry (DPV) in a mixed solution of D- and L-cystine. In addition, the DPV technique was used for the determination of D- and L-cystine at low concentration values in the range of 1.0–10.0 μM. To investigate the amperometric response of rGO/β-CD/GCE towards D- and L-cystine, the chronoamperometry technique was used in the concentration range of 10.0–100.0 μM. The interactions of the enantiomers with rGO/β-CD were modelled by molecular docking using AutoDock Vina, and the interaction energies were predicted to be −4.8 and −5.3 kcal mol⁻¹ for D- and L-cystine, respectively. The corresponding values of binding constants were calculated to be 3.32 × 10³ and 7.71 × 10³ M⁻¹, respectively. The experimental and molecular docking results indicate that the rGO/β-CD/GCE has a different affinity for each enantiomer.

We report on fluorometric and voltammetric detection of L-methionine (Met) based on host–guest interactions between Met and reduced graphene oxide/α-cyclodextrin (rGO/α-CD) hybrid materials. For voltammetric measurements, rGO/α-CD was used as an electrode modification material and successful detection of Met was achieved. Also, rGO/α-CD was used as a fluorescence quencher (turn off) for luminol which was employed as a fluorescent probe. The addition of Met into the same solution gave rise to fluorescence enhancement (turn on) after the host–guest recognition between Met and rGO/α-CD which caused the release of luminol. Interactions of luminol and Met with rGO/α-CD were modeled by molecular docking using AutoDock Vina and the interaction energies were predicted to be −4.3 and −4.4 kcal mol⁻¹, respectively. The proposed biosensor is considered to be a promising model for the detection of Met.