Background: Essential hypertension (EH) is a complex disease as a consequence of interaction between environmental factors and genetic background, but the pathogenesis of EH remains elusive. The emerging tools of network medicine offer a platform to explore a complex disease at system level. In this study, we aimed to identify the key proteins and the biological regulatory pathways involving in EH and further to explore the molecular connectivities between these pathways by the topological analysis of the Protein-protein interaction (PPI) network. Result: The extended network including one giant network consisted of 535 nodes connected via 2572 edges and two separated small networks. 27 proteins with high BC and 28 proteins with large degree have been identified. NOS3 with highest BC and Closeness centrality located in the centre of the network. The backbone network derived from high BC proteins presents a clear and visual overview which shows all important regulatory pathways for blood pressure (BP) and the crosstalk between them. Finally, the robustness of NOS3 as central protein and accuracy of backbone were validated by 287 test networks. Conclusion: Our finding suggests that blood pressure variation is orchestrated by an integrated PPI network centered on NOS3.

The cluster of pneumonia cases linked to coronavirus disease 2019 (Covid-19), first reported in China in late December 2019 raised global concern, particularly as the cumulative number of cases reported between 10 January and 5 March 2020 reached 80,711. In order to better understand the spread of this new virus, we characterized the spatial patterns of Covid-19 cumulative cases using ArcGIS v.10.4.1 based on spatial autocorrelation and cluster analysis using Global Moran’s I (Moran, 1950), Local Moran’s I and Getis-Ord General G (Ord and Getis, 2001). Up to 5 March 2020, Hubei Province, the origin of the Covid-19 epidemic, had reported 67,592 Covid-19 cases, while the confirmed cases in the surrounding provinces Guangdong, Henan, Zhejiang and Hunan were 1351, 1272, 1215 and 1018, respectively. The top five regions with respect to incidence were the following provinces: Hubei (11.423/10,000), Zhejiang (0.212/10,000), Jiangxi (0.201/10,000), Beijing (0.196/10,000) and Chongqing (0.186/10,000). Global Moran’s I analysis results showed that the incidence of Covid-19 is not negatively correlated in space (p=0.407413>0.05) and the High-Low cluster analysis demonstrated that there were no high-value incidence clusters (p=0.076098>0.05), while Local Moran’s I analysis indicated that Hubei is the only province with High-Low aggregation (p<0.0001).

Background: As a member of m6A methylated binding protein, RNA binding motif protein X-linked (RBMX) has been reported to be associated with tumor invasion, metastasis and prognosis. However, the prognostic significance of RBMX expression in esophageal cancer (ESCA) remains unclear. Methods: Based on the TIMER database, GEPIA database, cBioPortal database, CIBERSORT deconvolution algorithm, String-DB database, LinkedOmics database, etc., the RBMX expression level, mRNA expression level, prognostic relationship, genetic mutation, immune cell infiltration level, protein interaction network, differential co-expression genes and functional enrichment in esophageal carcinoma were analyzed. Immunohistochemistry was used to detect the expression of RBMX in 53 cases of esophageal carcinoma and adjacent esophageal tissues. Results: The RBMX expression in ESCA tissue was significantly higher than that in the normal tissues. The overall survival (OS) of patients with high RBMX expression was significantly lower than that of patients with low expression (P=0.04). The protein encoded by the RBMX gene appeared to copy number amplification, mutation and deep deletion. The expression level of RBMX was positively correlated with the levels of follicular helper T cells, eosinophils and initial B cells (P<0.05). Genes significantly and positively correlated with RBMX expression included HNRNPA1L2, SFRS13A, HNRNPA1, etc., which were mainly enriched in biological processes (BPs) such as cell division, mRNA splicing, RNA binding and mRNA 3'-UTR binding. Conclusions: RBMX may be as a biomarker of poor prognosis of ESCA. RBMX is closely related to the survival and prognosis, genetic mutation and immune cell infiltration of patients with ESCA.

Background: Since the first appearance in Wuhan, China in December 2019, the novel coronavirus disease (COVID-19) has posed serious threats to the public health in many Chinese places and overseas. It is essential to quantify the transmissibility on real-time basis for designing public health responses. Methods: We estimated the time-varying reproduction numbers in China, Hubei province and Wuhan city by using the renewable equation determined by the serial interval (SI) of COVID-19. We compare the average reproduction numbers in different periods of time to explore the effectiveness of the public health control measures against the COVID-19 epidemic. Results: We estimated the reproduction numbers at 2.61 (95% CI: 2.47-2.75), 2.76 (95% CI: 2.54-2.95) and 2.71 (95% CI: 2.43-3.01) for China, Hubei province and Wuhan respectively. We found that the reproduction number largely dropped after the city lockdown. As of February 16, the three reproduction numbers further reduced to 0.98, 1.14 and 1.41 respectively. Conclusions: The control of COVID-19 epidemic was effective in substantially reducing the disease transmissibility in terms of the reproduction number in China reduced to 0.98 as of February 16. At the same time, the reproduction number in Wuhan was probably still larger than 1, and thus the enhancement in the public health control was recommended to maintain.

The occurrence and development of tumors involve a series of life activities of cells, among which cell death has always been a crucial part in the research of tumor mechanisms and treatment methods. Ferroptosis is a non-apoptotic form of cell death, which is characterized by lipid peroxidation accumulation and further cell membrane rupture caused by excessive production of intracellular oxygen free radicals dependent on iron ions. Esophageal cancer is one of the common digestive tract tumors. Patients in the early stage are mainly treated with surgery, and the curative effect is awe-inspiring. However, surgery is far from enough for terminal patients, and it is the best choice to combine radiotherapy and chemotherapy before the operation or during the perioperative period. Although the treatment plan for patients with advanced esophageal cancer is constantly being optimized, we are disappointed at the still meager 5-year survival rate of patients and the poor quality of life. A series of complex problems, such as increased chemotherapy drug resistance and decreased radiotherapy sensitivity of esophageal cancer cells, are waiting for us to tackle. Perhaps ferroptosis can provide practical and feasible solutions and bring new hope to patients with advanced esophageal cancer. The occurrence of ferroptosis is related to the dysregulation of iron metabolism, lipid metabolism, and glutamate metabolism. Therefore, these dysregulated metabolic participant proteins and signaling pathways are essential entry points for using cellular ferroptosis to resist the occurrence and development of cancer cells. This review first introduced the main regulatory mechanisms of ferroptosis. It then summarized the current research status of ferroptosis in esophageal cancer, expecting to provide ideas for the research related to ferroptosis in esophageal cancer.

Objective: The influence of age, period, and cohort on Hepatitis B (HB) incidence in four prefectures of southern Xinjiang, China is still not clear. This paper aims to analyze the long-term trend of the HB incidence in four prefectures of southern Xinjiang, China and to estimate the independent impact of age, period and cohort, as well as to predict the development trend of HB incidence in male and female groups, then to identify the targeted population for HB screening by the model fitting and prediction. Method: The data were from the Case List of HB Cases Reported in the Infectious Disease Reporting Information Management System and the Xinjiang Statistical Yearbook of China. The age-period-cohort (APC) model was used to estimate the impacts of age, period and cohort on HB incidence, which could be used to predict the HB incidence in specific age groups of men and women. Results: Under the influence of age effect, the incidence of HB in males had two peaks (20–35 years old and 60–80 years old), the influence of age effect on the incidence of HB in females was lower than that of males and the obvious peak was between 20–30 years old; the period effect on the HB incidence in males and females fluctuated greatly and the fluctuation degree of influence on males was bigger than that of women. The HB incidence among males and females in the four regions tended to be affected by cohort effect, which reached a peak after 1990 and then declined sharply and gradually became stabilized. By predicting the HB incidence from 2018 to 2022, we found that there were significant differences in HB incidence among people over 35 years old, under 35 years old and the whole population in four prefectures of southern Xinjiang, China. Conclusions: Although the incidence of HB in some regions shows a downward trend, there is still an obvious upward trend of incidences in other places. In our paper, results indicate that the burden of HB incidence may be extended in the future, so we hope this can draw the attention of relative departments. These results reveal the differences of incidence between males and females as well, so respective measures of the two groups’ functions are essential.