This study aimed to investigate the role of chitosan oligosaccharide (COS) as an additive in the feed of laying hens with fatty liver syndrome (FLS). Effects on production performance, egg quality as well as ovarian function were determined. A total of 360 Lohmann Pink-shell laying hens (28 weeks old) were randomly assigned to 5 groups (6 replicates × 12 birds). Hens were fed with a basal diet and a high-energy low-protein (HELP) diet supplemented with 0, 200, 400 and 800 mg/kg COS. COS reversed the lowered laying rates, increased feed-to-egg ratios and decreased albumen heights and Haugh units induced by the HELP diet. Additionally, COS improved the ovarian morphologies damaged by the HELP diet. Furthermore, COS enhanced antioxidant enzyme activities, reduced malonaldehyde levels and downregulated the mRNA expressions of nuclear factor kappa B, pro-inflammation cytokine genes and pro-apoptosis-related genes, while it upregulated the mRNA expression of anti-apoptosis-related genes in the ovaries of HELP-diet-fed hens. These findings suggested that dietary COS supplementation could improve production performance and egg quality in laying hens with FLS, and these beneficial effects were linked to improved ovarian morphology, which was attributed to decreased oxidative stress, inflammation and apoptosis in the ovaries.

To obtain high-quality silage and better understand the mechanism underlying silage fermentation, a study was conducted to investigate the effects of konjac flour (KF), Lactiplantibacillus plantarum (LP) and their combination on fermentation quality, aerobic stability, and microbial community of high-moisture forage rape after 60 days of ensiling. Results showed that the KF and LP treatments increased the lactic acid content, decreased the pH value, and inhibited the production of butyric acid in ensiled forage rape (p < 0.05). The additives also altered the bacterial community of forage rape silages, showing reduced Shannon and Simpson indexes (p < 0.05), while the abundance of desirable Lactobacillus was increased, and the abundance of undesirable bacteria, such as enterobacteria and clostridia, was decreased (p < 0.05). In addition, their combination significantly improved the aerobic stability (96 h vs. 28 h, p < 0.05) and exhibited notable influence on the bacterial community, with the highest abundance of Lactobacillus. These results indicated that KF and LP improved the silage quality of high-moisture forage rape, and their combination displayed a beneficial synergistic effect.