Gold nanoparticles (GNPs) emerged as promising antitumor radiosensitizers. However, the complex dependence of GNPs radiosensitization on the irradiation conditions remains unclear. In the present study, we investigated the impacts of the dose rate and photon energy on damage of the pBR322 plasmid DNA exposed to X-rays in the presence of 12 nm, 15 nm, 21 nm, and 26 nm GNPs. The greatest radiosensitization was observed for 26 nm GNPs. The sensitizer enhancement ratio (SER) 2.74 ± 0.61 was observed at 200 kVp with 2.4 mg/mL GNPs. Reduction of X-ray tube voltage to 150 and 100 kVp led to a smaller effect. We demonstrate for the first time that the change of the dose rate differentially influences on radiosensitization by GNPs of various sizes. For 12 nm, an increase in the dose rate from 0.2 to 2.1 Gy/min led to a ~1.13-fold increase in radiosensitization. No differences in the effect of 15 nm GNPs was found within the 0.85–2.1 Gy/min range. For 21 nm and 26 nm GNPs, an enhanced radiosensitization was observed along with the decreased dose rate from 2.1 to 0.2 Gy/min. Thus, GNPs are an effective tool for increasing the efficacy of orthovoltage X-ray exposure. However, careful selection of irradiation conditions is a key prerequisite for optimal radiosensitization efficacy.

Simple procedures for Ta₂O₅ nanoparticle (NP) synthesis and surfactant-free stable hydrosol preparation were developed in order to investigate the colloidal properties, cytotoxicity, and CT contrast performance of uncoated Ta₂O₅ NPs.

Nanoparticle (NP) assisted magnetic hyperthermia (NMH) is a clinically proven method for cancer treatment. High-Z magnetic NPs could also be a perspective object for combining hyperthermia with tumor radiosensitization. However, this application of NPs is little studied, and it is unclear as to what particle compositions one can rely on. Therefore, the present work focuses on the search of materials that combine alternating magnetic field induced heating and high atomic number related dose enhancement abilities. A theoretical evaluation of 24 promising NP compositions was performed: the values of dose enhancement factor (DEF) were determined for kilovoltage x-ray spectra (30–300 kVp), as well as specific absorption rate (SAR) values were calculated for various combinations of elemental compositions and particle size distributions. For the alternating magnetic fields with amplitude $75\text{–}200\mathrm{Oe}$ and frequency $100\mathrm{kHz}$ , the maximum obtained SAR values ranged from 0.35 to $6000\mathrm{W}{\mathrm{g}}^{-1}$ , while DEF values for studied compounds ranged from 1.07 to 1.59. The increase in the monodispersity of NPs led to a higher SAR, confirming well-known experimental data. The four types of SAR dependences on external magnetic field amplitude and anisotropy constant were found for various particle sizes. The most predictable SAR behavior corresponds to larger NPs (∼70–100 nm). Thus, based on these calculations, the most promising for the combination of NMH with radiotherapy, from a physical point of view, are $\mathrm{L}{\mathrm{a}}_{0.75}\mathrm{S}{\mathrm{r}}_{0.25}\mathrm{Mn}{\mathrm{O}}_3$ , $\mathrm{G}{\mathrm{d}}_5\mathrm{S}{\mathrm{i}}_4$ , $\mathrm{SmC}{\mathrm{o}}_5$ , and $\mathrm{F}{\mathrm{e}}_{50}\mathrm{R}{\mathrm{h}}_{50}$ . The greatest dose enhancement is expected for superficial radiotherapy (in the voltage range up to ∼60 kVp).

Nanoparticles (NPs) with a high atomic number (Z) are promising radiosensitizers for cancer therapy. However, the dependence of their efficacy on irradiation conditions is still unclear. In the present work, 11 different metal and metal oxide NPs (from Cu (Z_Cu = 29) to Bi₂O₃ (Z_Bi = 83)) were studied in terms of their ability to enhance the absorbed dose in combination with 237 X-ray spectra generated at a 30–300 kVp voltage using various filtration systems and anode materials. Among the studied high-Z NP materials, gold was the absolute leader by a dose enhancement factor (DEF; up to 2.51), while HfO₂ and Ta₂O₅ were the most versatile because of the largest high-DEF region in coordinates U (voltage) and E_eff (effective energy). Several impacts of the X-ray spectral composition have been noted, as follows: (1) there are radiation sources that correspond to extremely low DEFs for all of the studied NPs, (2) NPs with a lower Z in some cases can equal or overcome by the DEF value the high-Z NPs, and (3) the change in the X-ray spectrum caused by a beam passing through the matter can significantly affect the DEF. All of these findings indicate the important role of carefully planning radiation exposure in the presence of high-Z NPs.

Bisbenzimidazoles have a broad spectrum of potential applications: radioprotectors, drug delivery vectors, antiviral agents, etc. At the same time, they seem to be promising fluorescent probes for radiation measurements. Therefore, in the present work, a fluorescent response to X-ray irradiation of Hoechst 33258, one of the most widely known representatives of the bisbenzimidazole family, was studied for the first time. Irradiation of the dye was performed in aqueous and organic solutions (DMSO and glycerol), as well as in their mixtures. It is shown that the reaction of the dye to radiation exposure is very versatile and may be controlled by the solvent properties, which makes it possible to build relationships between the absorbed dose and a wide variety of parameters of its fluorescence signal. For example, irradiation may induce fluorescence quenching caused by the degradation of the dye, a change in the position of the fluorescence band maximum due to the modification of the dye molecules or to the radiation-induced changes in the properties of the medium, as well as a fluorescence flare-up mediated by the changes in pH.