Therapy dogs are increasingly being incorporated into numerous clinical settings. However, there are only a handful of studies that have focused on the impact of animal-assisted activity or therapy sessions on the wellbeing of the therapy dogs. Furthermore, these studies show mixed results. The goal of this study was to provide an in-depth picture of the effects of these interactions on the dogs involved by considering multiple physiological measures known to be associated with emotional state (continuous heart rate, heart rate variability, pre- and post-session tympanic membrane temperatures, and salivary cortisol and oxytocin concentrations). Nineteen Mayo Clinic Caring Canine therapy dogs completed five 20-minute animal-assisted activity (AAA) visits each in an outpatient clinical setting (Mayo Clinic Fibromyalgia and Chronic Fatigue Clinic). From a physiological perspective, the dogs showed a neutral to positive response to the AAA sessions. Heart rate (HR) was significantly lower at the end of the session compared with the beginning of the session (F = 17.26, df1 = 1, df2 = 29.7, p = 0.0003). The right tympanic membrane temperature was lower post-session (F = 8.87, df1 = 1, df2 = 107, p = 0.003). All other emotional indicators remained stable between pre- and post-session. These results suggest that the dogs involved were not negatively affected by their participation in the AAA. Moreover, there was some evidence suggesting the dogs may have been in a more relaxed state at the end of the session (lower HR and lower right tympanic membrane temperature) compared to the beginning of the session.

Oxytocin (OT) is a mammalian neuropeptide with various functions in regulating birth, lactation, parenting, and social recognition. The study of OT became of increasing interest for the petcare industry due to its role in animal behavior and socialization. In the present study, a simple, sensitive, and accurate liquid chromatography-mass spectrometry (LC-MS) method for quantifying OT in dog saliva was developed and validated. OT and its deuterated internal standard (OT-d₅) were detected with multiple reaction monitoring (MRM) in a positive ion mode using an AB Sciex 6500+ QTRAP mass spectrometer. Chromatographic separation was achieved by using an ACE Excel C18 column and a gradient elution at a flow rate of 0.5 mL/min over a 5 min run. The mobile phases consisted of 0.1% (v/v) acetic acid in water and 0.1% (v/v) acetic acid in acetonitrile. After development and optimization, the performance of the method was validated to prove its reliability. Calibration curves were linear over the range of 50–20,000 pg/mL and recovery of OT was above 87.8%. The validated method was successfully applied to evaluate OT concentrations in multiple batches of dog saliva samples.