Plant pathogens cause significant losses to agricultural yields and increasingly threaten food security¹, ecosystem integrity and societies in general^2,3,4,5. Xylella fastidiosa is one of the most dangerous plant bacteria worldwide, causing several diseases with profound impacts on agriculture and the environment⁶. Primarily occurring in the Americas, its recent discovery in Asia and Europe demonstrates that X. fastidiosa’s geographic range has broadened considerably, positioning it as a reemerging global threat that has caused socioeconomic and cultural damage^7,8. X. fastidiosa can infect more than 350 plant species worldwide⁹, and early detection is critical for its eradication⁸. In this article, we show that changes in plant functional traits retrieved from airborne imaging spectroscopy and thermography can reveal X. fastidiosa infection in olive trees before symptoms are visible. We obtained accuracies of disease detection, confirmed by quantitative polymerase chain reaction, exceeding 80% when high-resolution fluorescence quantified by three-dimensional simulations and thermal stress indicators were coupled with photosynthetic traits sensitive to rapid pigment dynamics and degradation. Moreover, we found that the visually asymptomatic trees originally scored as affected by spectral plant-trait alterations, developed X. fastidiosa symptoms at almost double the rate of the asymptomatic trees classified as not affected by remote sensing. We demonstrate that spectral plant-trait alterations caused by X. fastidiosa infection are detectable previsually at the landscape scale, a critical requirement to help eradicate some of the most devastating plant diseases worldwide.

Automatic methods for an early detection of plant diseases (i.e., visible symptoms at early stages of disease development) using remote sensing are critical for precision crop protection. Verticillium wilt (VW) of olive caused by Verticillium dahliae can be controlled only if detected at early stages of development. Linear discriminant analysis (LDA) and support vector machine (SVM) classification methods were applied to classify V. dahliae severity using remote sensing at large scale. High-resolution thermal and hyperspectral imagery were acquired with a manned platform which flew a 3000-ha commercial olive area. LDA reached an overall accuracy of 59.0% and a κ of 0.487 while SVM obtained a higher overall accuracy, 79.2% with a similar κ, 0.495. However, LDA better classified trees at initial and low severity levels, reaching accuracies of 71.4 and 75.0%, respectively, in comparison with the 14.3% and 40.6% obtained by SVM. Normalized canopy temperature, chlorophyll fluorescence, structural, xanthophyll, chlorophyll, carotenoid and disease indices were found to be the best indicators for early and advanced stage infection by VW. These results demonstrate that the methods developed in other studies at orchard scale are valid for flights in large areas comprising several olive orchards differing in soil and crop management characteristics.

Red leaf blotch is one of the major fungal foliar diseases affecting almond orchards. High-resolution thermal and hyperspectral airborne imagery was acquired from two flights and compared with concurrent field visual evaluations for disease incidence and severity. Canopy temperature and vegetation indices were calculated from thermal and hyperspectral imagery and analyzed for their ability to detect the disease at early stages. The classification methods linear discriminant analysis and support vector machine, using linear and radial basis kernels, were applied to a combination of these vegetation indices in order to quantify and discriminate between red leaf blotch severity levels. Chlorophyll and carotenoid indices and chlorophyll fluorescence were effective in detecting red leaf blotch at the early stages of disease development. Linear models showed higher power to separate between asymptomatic trees and those affected by advanced stages of disease development while the non-linear model was better in discriminating asymptomatic plants from those at early stages of red leaf blotch development. Leaf-level measurements of stomatal conductance, chlorophyll content, chlorophyll fluorescence, photochemical reflectance index, and spectral reflectance showed no significant differences between healthy leaves and the green areas of symptomatic leaves. This study demonstrated the feasibility of early detecting and quantifying red leaf blotch using high-resolution hyperspectral imagery.

Plant pathogens pose increasing threats to global food security, causing yield losses that exceed 30% in food-deficit regions. Xylella fastidiosa (Xf) represents the major transboundary plant pest and one of the world’s most damaging pathogens in terms of socioeconomic impact. Spectral screening methods are critical to detect non-visual symptoms of early infection and prevent spread. However, the subtle pathogen-induced physiological alterations that are spectrally detectable are entangled with the dynamics of abiotic stresses. Here, using airborne spectroscopy and thermal scanning of areas covering more than one million trees of different species, infections and water stress levels, we reveal the existence of divergent pathogen- and host-specific spectral pathways that can disentangle biotic-induced symptoms. We demonstrate that uncoupling this biotic–abiotic spectral dynamics diminishes the uncertainty in the Xf detection to below 6% across different hosts. Assessing these deviating pathways against another harmful vascular pathogen that produces analogous symptoms, Verticillium dahliae, the divergent routes remained pathogen- and host-specific, revealing detection accuracies exceeding 92% across pathosystems. These urgently needed hyperspectral methods advance early detection of devastating pathogens to reduce the billions in crop losses worldwide.