Alfalfa is planted in more than 30 million hectares worldwide, but despite its popularity in temperate regions, it is not widely grown in subtropical agroecosystems. It is critical to improve alfalfa for such regions, considering current predictions of global warming and the increasing demands for animal-based products. In this study, we examined the diversity present in subtropical alfalfa germplasm and reported genetic parameters for forage production. An initial screening was performed from 2014 to 2016, evaluating 121 populations from different subtropical origins. Then, a breeding population was created by crossing selected plants, resulting in 145 full-sib and 36 half-sib families, which were planted in a row-column design with augmented representation of three controls (‘Bulldog805′, ‘FL99′ and ‘UF2015′). Dry matter yield (DMY), canopy height (AH), and percentage blooming (BLOOM) were measured across several harvests. Moderate narrow-sense heritability and high genetic correlations between consecutive harvests were estimated for all traits. The breeding line UF2015 produced higher DMY than FL99 and Bulldog805, and it could be a candidate cultivar release. Several families produced higher DMY than all checks, and they can be utilized to develop high yielding and adapted alfalfa cultivars for subtropical agroecosystems.

Bermudagrass (Cynodon spp.) is a forage and turf crop commonly used worldwide. The USDA bermudagrass germplasm set is composed of plant introductions (PI’s) collected around the world and contains different Cynodon species, primarily C. dactylon. The collection was screened in a replicated trial in Florida for forage yield, leaf width, nutritive value (NV), and Bermudagrass Stem Maggot (Atherigona reversura) (BSM), which is an invasive pest to the southeastern United States that damages bermudagrass fields. The goal of this research was to determine ploidy level and genome size in this USDA collection, and evaluate the influence of ploidy level in the estimation of genetic parameters for BSM, leaf width, dry matter yield, and NV traits. For chromosome counts using classical cytogenetics techniques, root tips and meristems were collected from a set of PI’s with known ploidy. The PI’s and cultivars with known chromosome counts were used as internal standards to run flow cytometry and estimate genome size of the PI’s with unknown ploidy. Ploidy level was determined for all accessions and were used to estimate genetic parameters of phenotypic traits. By providing information on ploidy levels and genetic parameters, this research will support breeding efforts and future selections for forage bermudagrass.

Spotted wilt, caused by tomato spotted wilt virus (TSWV), has been one of major diseases in cultivated peanut grown in the southeastern United States (US) since 1990. Previously a major quantitative trait locus (QTL) controlling spotted wilt disease resistance was mapped to an interval of 2.55 cM genetic distance corresponding to a physical distance of 14.4 Mb on chromosome A01 of peanut by using a segregating F₂ population. The current study focuses on refining this major QTL region and evaluating its contributions in the US peanut mini-core germplasm. Two simple sequence repeat (SSR) markers associated with the major QTL were used to genotype F₅ individuals, and 25 heterozygous individuals were selected and developed into an F₆ segregating population. Based on visual evaluation in the field, a total of 194 susceptible F₆ individuals were selected and planted into F₇ generation for phenotyping. Nine SSR markers were used to genotype the 194 F₆ individuals, and QTL analysis revealed that a confidence interval of 15.2 Mb region had the QTL with 22.8% phenotypic variation explained (PVE). This QTL interval was further genotyped using the Amplicon-seq method. A total of 81 non-redundant single nucleotide polymorphism (SNP) and eight InDel markers were detected. No recombinant was detected among the F₆ individuals. Two InDel markers were integrated into the linkage group and helped to refine the confidence interval of this QTL into a 0.8 Mb region. To test the QTL contributes to the resistance variance in US peanut mini-core germplasm, two flanking SSR markers were used to genotype 107 mini-core germplasm accessions. No statistically significant association was observed between the genotype at the QTL region and spotted wilt resistance in the mini-core germplasm, which indicated that the resistance allelic region at this QTL didn’t contribute to the resistance variance in the US peanut mini-core germplasm, thus was a unique resistance source. A major QTL related to spotted wilt disease resistance in peanut was refined to a 0.8 Mb region on A01 chromosome, which didn’t relate to spotted wilt disease resistance in the US peanut mini-core germplasm and might be a unique genetic source.

The Cynodon spp. collection maintained by United States Department of Agriculture National Plant Germplasm System (USDA-NPGS) has limited information on nutritive value (NV) traits. In this study, crude protein (CP), phosphorous concentration (P), in vitro digestible organic matter (IVDOM), and neutral detergent fiber (NDF) were determined to (i) estimate genetic parameters for NV, (ii) obtain genetic values for the whole population across two harvests, (iii) estimate genotype by harvest interaction (GHI) for NV traits, and (iv) select accessions exhibiting improved NV traits compared to ‘Tifton 85′. The experiment was setup as a row-column design with two replicates and augmented representation of controls: Tifton 85, ‘Jiggs’, and ‘Coastal’. The whole-population was harvested twice, and data were analyzed using linear mixed models with repeated measures. In addition, a selected population of 15 genotypes were evaluated across 11 harvests to determine the extent of GHI. Genetic parameters revealed the presence of significant genetic variability, indicating potential improvements for NV through breeding. Specifically, P and IVDOM presented large variation, while NDF had lower diversity but some accessions exhibited lower NDF than Tifton 85. Low GHI, except for IVDOM, indicated genotypic stability and potential for selecting improved accessions under fewer harvests. Breeding line 240, PI-316510, and PI-3166536 presented superior NV than Tifton 85.