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The mechanisms allowing the rapid release of stored water to streams are poorly understood. Here we use a tile-drained field site to combine macroporous soils at the hillslope scale with the advantage of at least partly controlled lower boundary conditions. We performed a series of three irrigation experiments combining hydrometric measurements with stable isotope and bromide tracers to better understand macropore–matrix interactions and stored water release processes at the hillslope scale. Stable isotope concentrations were monitored in the irrigation water, the tile-drain discharge and the soil water before and after the experiment. Bromide was measured every 5–15 min in the tile-drain hydrograph. Different initial conditions for each experiment were used to examine how these influenced flow and transport. Different amounts of irrigation water were necessary to increase tile-drain discharge above the baseflow level. Hydrograph separation based on bromide data revealed that irrigation water contributions to peak tile-drain discharge were on the order of 20%. Oxygen-18 and deuterium data were consistent with the bromide data and showed that pre-event soil water contributed significantly to the tile-drain event flow. However, the isotopic composition of soil water converged towards the isotopic composition of irrigation water through the course of the experiment. Mixing calculations revealed that by the end of the irrigation experiments 20% of the soil water in the entire profile was irrigation water. The isotopic data showed that the pre-event water in the tile drain was mobilized in 20–40 cm soil depth where the macropore–matrix interaction leads to an initiation of macropore flow after a moisture threshold is exceeded.

Predicting and understanding subsurface flowpaths is still a crucial issue in hydrological research. We present an experimental approach to reveal present and past subsurface flowpaths of water in the unsaturated and saturated zone. Two hillslopes in a humid mountainous catchment have been investigated. The H₂O_(liquid) – H₂O_(vapor) equilibration laser spectroscopy method was used to obtain high resolution δ²H vertical depth profiles of pore water at various points along two fall lines of a pasture hillslope in the southern Black Forest, Germany. The Porewater-based Stable Isotope Profile (PSIP) approach was developed to use the integrated information of several vertical depth profiles of deuterium along transects at the hillslope. Different shapes of depth profiles were observed in relation to hillslope position. The statistical variability (inter-quartile range and standard deviation) of each profile was used to characterize different types of depth profiles. The profiles upslope or with a weak affinity for saturation as indicated by a low topographic wetness index preserve the isotopic input signal by precipitation with a distinct seasonal variability. These observations indicate mainly vertical movement of soil water in the upper part of the hillslope before sampling. The profiles downslope or at locations with a strong affinity for saturation do not show a similar seasonal isotopic signal. The input signal is erased in the foothills and a large proportion of pore water samples are close to the isotopic values of δ²H in streamwater during base flow conditions indicating the importance of the groundwater component in the catchment. Near the stream indications for efficient mixing of water from lateral subsurface flow paths with vertical percolation are found.