Water Framework Directive requires reliable and effective monitoring tools, and hydroacoustics has a potential to be one of them. The effect of pulse duration on in situ acoustical estimates of fish density and their size distribution was investigated. Measurements were performed in the oligo-mesotrophic Lake Hancza (Poland) using a SIMRAD EK60 split-beam echo-sounder at 70 kHz frequency. During the survey, two similar transducers pinged alternatively through the multiplexer using 4 different pulse lengths, from short to long ones. The results show that the volume backscattering coefficient (Sv) values, equivalent of the fish biomass, were not influenced by the pulse length. However, the number of the detected fish, the mean target strength (TS), and consequently the fish density, differed significantly for the long pulse duration data. This was especially noticeable in the layer above the thermocline with dense fish populations. In this upper layer, for the long pulse the Sawada index frequently exceeded value of 0.1 leading to overestimation of the mean TS and underestimation of the fish density.

Several decades of research have led to the acceptance of hydroacoustics as a reliable measurement method to monitor fish population in lakes, but full standardisation and intercalibration are still lacking. The aim of this study was to investigate the effect of sound frequency on acoustic parameters, such as volume backscattering strength, target strength, and the estimation of fish abundance. Data were recorded in situ using 3 frequencies (70, 120, 200 kHz) simultaneously in 2 different lakes. The results among the frequencies were compared and statistically tested. Data from the 70 and 120 kHz frequencies yielded similar results, but the 200 kHz echosounder estimates in temperate lakes seemed different, especially in cases of high fish abundance, which is typical of eutrophic ecosystems. This work indicates that the abundance estimates of fish populations in temperate lakes based on 200 kHz frequency may differ from results obtained using lower frequencies, and that further study is needed.