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Maurilia Marcacci
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, Italy

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Communication
Published: 31 March 2021 in Microorganisms
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Listeria monocytogenes is a bacterial pathogen responsible of listeriosis, a disease that in humans is often related to the contamination of ready-to-eat foods. Phages are candidate biodecontaminants of pathogenic bacteria thanks to their ability to lyse prokaryotes while being safe for eukaryotic cells. In this study, ΦIZSAM-1 was isolated from the drain-waters of an Italian blue cheese plant and showed lytic activity against antimicrobial resistant Listeria monocytogenes strains. This phage was subjected to purification and in vitro efficacy tests. The results showed that at multiplicities of infection (MOIs) ≤ 1, phages were able to keep Listeria monocytogenes at low optical density values up to 8 h, with bacterial counts ranging from 1.02 to 3.96 log10 units lower than the control. Besides, ΦIZSAM-1 was further characterized, showing 25 principal proteins (sodium dodecyl sulfate polyacrylamide gel electrophoresis profile) and a genome of approximately 50 kilo base pairs. Moreover, this study describes a new approach to phage isolation for applications in Listeria monocytogenes biocontrol in food production. In particular, the authors believe that the selection of phages from the same environments where pathogens live could represent a new approach to successfully integrating the control measures in an innovative, cost effective, safe and environmentally friendly way.

ACS Style

Silvia Scattolini; Daniela D’Angelantonio; Arianna Boni; Iolanda Mangone; Maurilia Marcacci; Noemi Battistelli; Krizia D’Agostino; Francesco Pomilio; Cesare Camma; Giacomo Migliorati; Giuseppe Aprea. Characterization and In Vitro Efficacy against Listeria monocytogenes of a Newly Isolated Bacteriophage, ΦIZSAM-1. Microorganisms 2021, 9, 731 .

AMA Style

Silvia Scattolini, Daniela D’Angelantonio, Arianna Boni, Iolanda Mangone, Maurilia Marcacci, Noemi Battistelli, Krizia D’Agostino, Francesco Pomilio, Cesare Camma, Giacomo Migliorati, Giuseppe Aprea. Characterization and In Vitro Efficacy against Listeria monocytogenes of a Newly Isolated Bacteriophage, ΦIZSAM-1. Microorganisms. 2021; 9 (4):731.

Chicago/Turabian Style

Silvia Scattolini; Daniela D’Angelantonio; Arianna Boni; Iolanda Mangone; Maurilia Marcacci; Noemi Battistelli; Krizia D’Agostino; Francesco Pomilio; Cesare Camma; Giacomo Migliorati; Giuseppe Aprea. 2021. "Characterization and In Vitro Efficacy against Listeria monocytogenes of a Newly Isolated Bacteriophage, ΦIZSAM-1." Microorganisms 9, no. 4: 731.

Brief report
Published: 16 December 2020 in Viruses
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Outbreaks of Rift Valley fever (RVF) occurred in Namibia in 2010 and 2011. Complete genome characterization was obtained from virus isolates collected during disease outbreaks in southern Namibia in 2010 and from wildlife in Etosha National Park in 2011, close to the area where RVF outbreaks occurred in domestic livestock. The virus strains were sequenced using Sanger sequencing (Namibia_2010) or next generation sequencing (Namibia_2011). A sequence-independent, single-primer amplification (SISPA) protocol was used in combination with the Illumina Next 500 sequencer. Phylogenetic analysis of the sequences of the small (S), medium (M), and large (L) genome segments of RVF virus (RVFV) provided evidence that two distinct RVFV strains circulated in the country. The strain collected in Namibia in 2010 is genetically similar to RVFV strains circulating in South Africa in 2009 and 2010, confirming that the outbreaks reported in the southern part of Namibia in 2010 were caused by possible dissemination of the infection from South Africa. Isolates collected in 2011 were close to RVFV isolates from 2010 collected in humans in Sudan and which belong to the large lineage containing RVFV strains that caused an outbreak in 2006–2008 in eastern Africa. This investigation showed that the RVFV strains circulating in Namibia in 2010 and 2011 were from two different introductions and that RVFV has the ability to move across regions. This supports the need for risk-based surveillance and monitoring.

ACS Style

Gian Mario Cosseddu; Kudakwashe Magwedere; Umberto Molini; Chiara Pinoni; Sigfried Khaiseb; Massimo Scacchia; Maurilia Marcacci; Andrea Capobianco Dondona; Fabrizia Valleriani; Andrea Polci; Federica Monaco. Genetic Diversity of Rift Valley Fever Strains Circulating in Namibia in 2010 and 2011. Viruses 2020, 12, 1453 .

AMA Style

Gian Mario Cosseddu, Kudakwashe Magwedere, Umberto Molini, Chiara Pinoni, Sigfried Khaiseb, Massimo Scacchia, Maurilia Marcacci, Andrea Capobianco Dondona, Fabrizia Valleriani, Andrea Polci, Federica Monaco. Genetic Diversity of Rift Valley Fever Strains Circulating in Namibia in 2010 and 2011. Viruses. 2020; 12 (12):1453.

Chicago/Turabian Style

Gian Mario Cosseddu; Kudakwashe Magwedere; Umberto Molini; Chiara Pinoni; Sigfried Khaiseb; Massimo Scacchia; Maurilia Marcacci; Andrea Capobianco Dondona; Fabrizia Valleriani; Andrea Polci; Federica Monaco. 2020. "Genetic Diversity of Rift Valley Fever Strains Circulating in Namibia in 2010 and 2011." Viruses 12, no. 12: 1453.

Journal article
Published: 14 December 2020 in Viruses
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Italy was one of the first countries to experience a major epidemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with >1000 cases confirmed by 1 March 2020. However, virus genome sequence data is sparse and there has been only limited investigation of virus transmission across the country. Here, we provide the most extensive study to date of the genomic epidemiology of SARS-CoV-2 in Italy covering the first wave of infection. We generated 191 new full-length genomes, largely sampled from central Italy (Abruzzo), before, during, and after the enforcement of a nationwide “lockdown” (8 March–3 June). These were combined with 460 published SARS-CoV-2 sequences sampled across Italy. Phylogenetic analysis including global sequence data revealed multiple independent introductions into Italy, with at least 124 instances of sequence clusters representing longer chains of transmission. Eighteen of these transmission clusters emerged before the nation-wide lockdown was implemented on 8 March, and an additional 18 had evidence for transmission between different Italian regions. Extended transmission periods between infections of up to 104 days were observed in five clusters. In addition, we found seven clusters that persisted throughout the lockdown period. Overall, we show how importations were an important driver of the first wave of SARS-CoV-2 in Italy.

ACS Style

Francesca Di Giallonardo; Sebastian Duchene; Ilaria Puglia; Valentina Curini; Francesca Profeta; Cesare Cammà; Maurilia Marcacci; Paolo Calistri; Edward C. Holmes; Alessio Lorusso. Genomic Epidemiology of the First Wave of SARS-CoV-2 in Italy. Viruses 2020, 12, 1438 .

AMA Style

Francesca Di Giallonardo, Sebastian Duchene, Ilaria Puglia, Valentina Curini, Francesca Profeta, Cesare Cammà, Maurilia Marcacci, Paolo Calistri, Edward C. Holmes, Alessio Lorusso. Genomic Epidemiology of the First Wave of SARS-CoV-2 in Italy. Viruses. 2020; 12 (12):1438.

Chicago/Turabian Style

Francesca Di Giallonardo; Sebastian Duchene; Ilaria Puglia; Valentina Curini; Francesca Profeta; Cesare Cammà; Maurilia Marcacci; Paolo Calistri; Edward C. Holmes; Alessio Lorusso. 2020. "Genomic Epidemiology of the First Wave of SARS-CoV-2 in Italy." Viruses 12, no. 12: 1438.

Journal article
Published: 11 September 2020 in Microorganisms
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Hepatitis E virus (HEV) is an emergent zoonotic pathogen, causing worldwide acute and chronic hepatitis in humans. HEV comprises eight genotypes and several subtypes. HEV genotypes 3 and 4 (HEV3 and HEV4) are zoonotic. In Italy, the most part of HEV infections (80%) is due to autochthonous HEV3 circulation of the virus, and the key role played by wild animals is generally accepted. Abruzzo is an Italian region officially considered an HEV “hot spot”, with subtype HEV3-c being up to now the only one reported among wild boars. During the year 2018–2019, a group of wild boars in Abruzzo were screened for HEV; positive RNA liver samples were subjected to HEV characterization by using the whole genome sequencing (WGS) approach methodology. This represents the first report about the detection of HEV-3 subtypes e and f in the wild boar population in this area. Since in Italy human infections from HEV 3-e and f have been associated with pork meat consumption, our findings deserve more in-depth analysis with the aim of evaluating any potential correlation between wild animals, the pork chain production and HEV human infections.

ACS Style

Giuseppe Aprea; Silvia Scattolini; Daniela D’Angelantonio; Alexandra Chiaverini; Valeria Di Lollo; Sabrina Olivieri; Maurilia Marcacci; Iolanda Mangone; Stefania Salucci; Salvatore Antoci; Cesare Cammà; Adriano Di Pasquale; Giacomo Migliorati; Francesco Pomilio. Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report. Microorganisms 2020, 8, 1393 .

AMA Style

Giuseppe Aprea, Silvia Scattolini, Daniela D’Angelantonio, Alexandra Chiaverini, Valeria Di Lollo, Sabrina Olivieri, Maurilia Marcacci, Iolanda Mangone, Stefania Salucci, Salvatore Antoci, Cesare Cammà, Adriano Di Pasquale, Giacomo Migliorati, Francesco Pomilio. Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report. Microorganisms. 2020; 8 (9):1393.

Chicago/Turabian Style

Giuseppe Aprea; Silvia Scattolini; Daniela D’Angelantonio; Alexandra Chiaverini; Valeria Di Lollo; Sabrina Olivieri; Maurilia Marcacci; Iolanda Mangone; Stefania Salucci; Salvatore Antoci; Cesare Cammà; Adriano Di Pasquale; Giacomo Migliorati; Francesco Pomilio. 2020. "Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report." Microorganisms 8, no. 9: 1393.

Brief report
Published: 23 July 2020 in Viruses
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Camelpox is a viral contagious disease of Old-World camelids sustained by Camelpox virus (CMLV). The disease is characterized by mild, local skin or severe systemic infections and may have a major economic impact due to significant losses in terms of morbidity and mortality, weight loss, and low milk yield. Prevention of camelpox is performed by vaccination. In this study, we investigated the composition of a CMLV-based, live-attenuated commercial vaccine using next-generation sequencing (NGS) technology. The results of this analysis revealed genomic sequences of Modified Vaccinia virus Ankara (MVA).

ACS Style

Maurilia Marcacci; Abdelmalik I. Khalafalla; Zulaikha M. Al Hammadi; Federica Monaco; Cesare Cammà; Mohammed F. Yusof; Saeed M. Al Yammahi; Iolanda Mangone; Fabrizia Valleriani; Mohamed A. Alhosani; Nicola DeCaro; Alessio Lorusso; Salama S. AlMuhairi; Giovanni Savini. Genome Sequencing of a Camelpox Vaccine Reveals Close Similarity to Modified Vaccinia virus Ankara (MVA). Viruses 2020, 12, 786 .

AMA Style

Maurilia Marcacci, Abdelmalik I. Khalafalla, Zulaikha M. Al Hammadi, Federica Monaco, Cesare Cammà, Mohammed F. Yusof, Saeed M. Al Yammahi, Iolanda Mangone, Fabrizia Valleriani, Mohamed A. Alhosani, Nicola DeCaro, Alessio Lorusso, Salama S. AlMuhairi, Giovanni Savini. Genome Sequencing of a Camelpox Vaccine Reveals Close Similarity to Modified Vaccinia virus Ankara (MVA). Viruses. 2020; 12 (8):786.

Chicago/Turabian Style

Maurilia Marcacci; Abdelmalik I. Khalafalla; Zulaikha M. Al Hammadi; Federica Monaco; Cesare Cammà; Mohammed F. Yusof; Saeed M. Al Yammahi; Iolanda Mangone; Fabrizia Valleriani; Mohamed A. Alhosani; Nicola DeCaro; Alessio Lorusso; Salama S. AlMuhairi; Giovanni Savini. 2020. "Genome Sequencing of a Camelpox Vaccine Reveals Close Similarity to Modified Vaccinia virus Ankara (MVA)." Viruses 12, no. 8: 786.

Journal article
Published: 11 May 2020 in Viruses
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In this study, starting from nucleic acids purified from the brain tissue, Nanopore technology was used to identify the etiological agent of severe neurological signs observed in a cow which was immediately slaughtered. Histological examination revealed acute non-suppurative encephalomyelitis affecting the brainstem, cerebrum, cerebellum, and medulla oblongata, while by using PCR-based assays, the nucleic acids of major agents for neurological signs were not detected. By using Nanopore technology, 151 sequence reads were assigned to Bovine Astrovirus (BoAstV). Real-time RT-PCR and in situ hybridization (ISH) confirmed the presence of viral RNA in the brain. Moreover, using the combination of fluorescent ISH and immunofluorescence (IF) techniques, it was possible to detect BoAstV RNA and antigens in the same cells, suggesting the active replication of the virus in infected neurons. The nearly whole genome of the occurring strain (BoAstV PE3373/2019/Italy), obtained by Illumina NextSeq 500, showed the highest nucleotide sequence identity (94.11%) with BoAstV CH13/NeuroS1 26,730 strain, an encephalitis-associated bovine astrovirus. Here, we provide further evidence of the role of AstV as a neurotropic agent. Considering that in a high proportion of non-suppurative encephalitis cases, which are mostly indicative of a viral infection, the etiologic agent remains unknown, our result underscores the value and versatility of Nanopore technology for a rapid diagnosis when the PCR-based algorithm gives negative results.

ACS Style

Guendalina Zaccaria; Alessio Lorusso; Melanie M. Hierweger; Daniela Malatesta; Sabrina Vp Defourny; Franco Ruggeri; Cesare Cammà; Pasquale Ricci; Marco Di Domenico; Antonio Rinaldi; Nicola DeCaro; Nicola D’Alterio; Antonio Petrini; Torsten Seuberlich; Maurilia Marcacci. Detection of Astrovirus in a Cow with Neurological Signs by Nanopore Technology, Italy. Viruses 2020, 12, 530 .

AMA Style

Guendalina Zaccaria, Alessio Lorusso, Melanie M. Hierweger, Daniela Malatesta, Sabrina Vp Defourny, Franco Ruggeri, Cesare Cammà, Pasquale Ricci, Marco Di Domenico, Antonio Rinaldi, Nicola DeCaro, Nicola D’Alterio, Antonio Petrini, Torsten Seuberlich, Maurilia Marcacci. Detection of Astrovirus in a Cow with Neurological Signs by Nanopore Technology, Italy. Viruses. 2020; 12 (5):530.

Chicago/Turabian Style

Guendalina Zaccaria; Alessio Lorusso; Melanie M. Hierweger; Daniela Malatesta; Sabrina Vp Defourny; Franco Ruggeri; Cesare Cammà; Pasquale Ricci; Marco Di Domenico; Antonio Rinaldi; Nicola DeCaro; Nicola D’Alterio; Antonio Petrini; Torsten Seuberlich; Maurilia Marcacci. 2020. "Detection of Astrovirus in a Cow with Neurological Signs by Nanopore Technology, Italy." Viruses 12, no. 5: 530.

Journal article
Published: 08 January 2020 in Foods
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From January 2015 to March 2016, an outbreak of 23 human cases of listeriosis in the Marche region and one human case in the Umbria region of Italy was caused by Listeria monocytogenes strains showing a new pulsotype never described before in Italy. A total of 37 clinical strains isolated from patients exhibiting listeriosis symptoms and 1374 strains correlated to the outbreak were received by the Italian National Reference Laboratory for L. monocytogenes (It NRL Lm) of Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) for outbreak investigation. A real-time PCR assay was purposely designed for a rapid screening of the strains related to the outbreak. PCR-positive strains were successively typed through molecular serogrouping, pulsed field gel electrophoresis (PFGE), and Next Generation Sequencing (NGS). Applying the described strategy, based on real-time PCR screening, we were able to considerably reduce time and costs during the outbreak investigation activities.

ACS Style

Marina Torresi; Anna Ruolo; Vicdalia Aniela Acciari; Massimo Ancora; Giuliana Blasi; Cesare Cammà; Patrizia Centorame; Gabriella Centorotola; Valentina Curini; Fabrizia Guidi; Maurilia Marcacci; Massimiliano Orsini; Francesco Pomilio; Marco Di Domenico. A Real-Time PCR Screening Assay for Rapid Detection of Listeria Monocytogenes Outbreak Strains. Foods 2020, 9, 67 .

AMA Style

Marina Torresi, Anna Ruolo, Vicdalia Aniela Acciari, Massimo Ancora, Giuliana Blasi, Cesare Cammà, Patrizia Centorame, Gabriella Centorotola, Valentina Curini, Fabrizia Guidi, Maurilia Marcacci, Massimiliano Orsini, Francesco Pomilio, Marco Di Domenico. A Real-Time PCR Screening Assay for Rapid Detection of Listeria Monocytogenes Outbreak Strains. Foods. 2020; 9 (1):67.

Chicago/Turabian Style

Marina Torresi; Anna Ruolo; Vicdalia Aniela Acciari; Massimo Ancora; Giuliana Blasi; Cesare Cammà; Patrizia Centorame; Gabriella Centorotola; Valentina Curini; Fabrizia Guidi; Maurilia Marcacci; Massimiliano Orsini; Francesco Pomilio; Marco Di Domenico. 2020. "A Real-Time PCR Screening Assay for Rapid Detection of Listeria Monocytogenes Outbreak Strains." Foods 9, no. 1: 67.

Journal article
Published: 02 April 2019 in Journal of Virological Methods
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Bluetongue virus (BTV) is a segmented double-stranded RNA virus, existing in multiple serotypes, belonging to the genus Orbivirus of the family Reoviridae. BTV causes Bluetongue (BT), a major OIE-listed disease of ruminants. Identification of BTV serotype is accomplished using multiple typing assays and tends to be executed based on the known epidemiological situation within a given country. Samples containing multiple serotypes, particularly those containing novel introductions, may therefore be missed. The aim of this work was to optimize the nCounter® Analysis System Microarray platform (NanoString technologies), that would simultaneously identify all BTV serotypes and co-infections in analyzed samples. Probes were designed according to all Seg-2 sequences, coding for VP2 proteins which determine serotype specificity, available on line. A specific BTV CodeSet of probes was optimized. Experiments were performed with 30 BTV isolates and with 46 field samples previously shown to be infected with BTV by classical molecular assays. All BTV isolates were correctly identified and the expected BTV serotype was recognized in 35 field samples with CT values between 22.0-33.0. In turn, it was unable to identify 11 samples with CT values between 29.0-38.0. Although specificity of the assay needs to be further investigated against a larger panel of BTVs collected worldwide, RNA loads, which are normally detected in blood samples during the acute phase of infection, are within the range of CT values detectable by the BTV CodeSet. We propose the NanoString RNA microarray as a first-line molecular diagnostic tool for identification and typing of BTV. Once identification of the index cases is performed, diagnosis of the following samples may be performed by specific, more sensitive and cheaper PCR-based tools.

ACS Style

Valentina Curini; Maurilia Marcacci; Alfreda Tonelli; Giovanni Di Teodoro; Marco Di Domenico; Nicola D’Alterio; Ottavio Portanti; Massimo Ancora; Giovanni Savini; Matteo Panfili; Cesare Cammà; Alessio Lorusso. Molecular typing of Bluetongue virus using the nCounter® analysis system platform. Journal of Virological Methods 2019, 269, 64 -69.

AMA Style

Valentina Curini, Maurilia Marcacci, Alfreda Tonelli, Giovanni Di Teodoro, Marco Di Domenico, Nicola D’Alterio, Ottavio Portanti, Massimo Ancora, Giovanni Savini, Matteo Panfili, Cesare Cammà, Alessio Lorusso. Molecular typing of Bluetongue virus using the nCounter® analysis system platform. Journal of Virological Methods. 2019; 269 ():64-69.

Chicago/Turabian Style

Valentina Curini; Maurilia Marcacci; Alfreda Tonelli; Giovanni Di Teodoro; Marco Di Domenico; Nicola D’Alterio; Ottavio Portanti; Massimo Ancora; Giovanni Savini; Matteo Panfili; Cesare Cammà; Alessio Lorusso. 2019. "Molecular typing of Bluetongue virus using the nCounter® analysis system platform." Journal of Virological Methods 269, no. : 64-69.

Short communication
Published: 25 February 2019 in Transboundary and Emerging Diseases
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Over the last twenty‐years, Italy has experienced multiple incursions of different serotypes of Bluetongue virus (BTV), a Culicoides‐borne arbovirus, the causative agent of Bluetongue (BT), a major disease of ruminants. The majority of these incursions originated from Northern Africa, likely because of wind‐blown dissemination of infected midges. Here we report the first identification of BTV‐3 in Sardinia, Italy. BTV‐3 circulation was evidenced in sentinel animals located in the province of Sud Sardegna on September 19th 2018. Prototype strain BTV‐3 SAR2018 was isolated on cell culture. BTV‐3 SAR2018 and partial sequences obtained by next generation sequencing from nucleic acids purified from the isolate and blood samples, respectively, were demonstrated to be almost identical (99‐100% of nucleotide identity) to BTV‐3 TUN2016 identified in Tunisia in 2016 and 2017, a scenario already observed in past incursions of other BTV serotypes originating from Northern Africa. This article is protected by copyright. All rights reserved.

ACS Style

Stefano Cappai; Sandro Rolesu; Federica Loi; Manuel Liciardi; Alessandra Leone; Maurilia Marcacci; Liana Teodori; Iolanda Mangone; Soufien Sghaier; Ottavio Portanti; Giovanni Savini; Alessio Lorusso. Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization. Transboundary and Emerging Diseases 2019, 66, 1426 -1431.

AMA Style

Stefano Cappai, Sandro Rolesu, Federica Loi, Manuel Liciardi, Alessandra Leone, Maurilia Marcacci, Liana Teodori, Iolanda Mangone, Soufien Sghaier, Ottavio Portanti, Giovanni Savini, Alessio Lorusso. Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization. Transboundary and Emerging Diseases. 2019; 66 (3):1426-1431.

Chicago/Turabian Style

Stefano Cappai; Sandro Rolesu; Federica Loi; Manuel Liciardi; Alessandra Leone; Maurilia Marcacci; Liana Teodori; Iolanda Mangone; Soufien Sghaier; Ottavio Portanti; Giovanni Savini; Alessio Lorusso. 2019. "Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization." Transboundary and Emerging Diseases 66, no. 3: 1426-1431.

Journal article
Published: 08 February 2019 in Scientific Reports
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Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis.

ACS Style

Alessia Peserico; Maurilia Marcacci; Daniela Malatesta; Marco Di Domenico; Annamaria Pratelli; Iolanda Mangone; Nicola D’Alterio; Federica Pizzurro; Francesco Cirone; Guendalina Zaccaria; Cesare Cammà; Alessio Lorusso. Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology. Scientific Reports 2019, 9, 1 -9.

AMA Style

Alessia Peserico, Maurilia Marcacci, Daniela Malatesta, Marco Di Domenico, Annamaria Pratelli, Iolanda Mangone, Nicola D’Alterio, Federica Pizzurro, Francesco Cirone, Guendalina Zaccaria, Cesare Cammà, Alessio Lorusso. Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology. Scientific Reports. 2019; 9 (1):1-9.

Chicago/Turabian Style

Alessia Peserico; Maurilia Marcacci; Daniela Malatesta; Marco Di Domenico; Annamaria Pratelli; Iolanda Mangone; Nicola D’Alterio; Federica Pizzurro; Francesco Cirone; Guendalina Zaccaria; Cesare Cammà; Alessio Lorusso. 2019. "Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology." Scientific Reports 9, no. 1: 1-9.

Brief report
Published: 13 December 2018 in Microbiology Resource Announcements
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We report the whole-genome sequence of a Listeria monocytogenes strain isolated from a child in central Italy. Interestingly, the sequence showed a difference of only 13 single-nucleotide polymorphisms (SNPs) from a strain responsible for a severe listeriosis outbreak that occurred between January 2015 and March 2016 in the same region.

ACS Style

Massimiliano Orsini; Marina Torresi; Claudio Patavino; Patrizia Centorame; Antonio Rinaldi; Vicdalia Aniela Acciari; Gabriella Centorotola; Maurilia Marcacci; Massimo Ancora; Marco Di Domenico; Giuliana Blasi; Anna Duranti; Samuel Perticara; Adriano Di Pasquale; Cesare Cammà; Francesco Pomilio; Giacomo Migliorati. Whole-Genome Sequence of a Reemerging Listeria monocytogenes Serovar 1/2a Strain in Central Italy. Microbiology Resource Announcements 2018, 7, e01069-18 .

AMA Style

Massimiliano Orsini, Marina Torresi, Claudio Patavino, Patrizia Centorame, Antonio Rinaldi, Vicdalia Aniela Acciari, Gabriella Centorotola, Maurilia Marcacci, Massimo Ancora, Marco Di Domenico, Giuliana Blasi, Anna Duranti, Samuel Perticara, Adriano Di Pasquale, Cesare Cammà, Francesco Pomilio, Giacomo Migliorati. Whole-Genome Sequence of a Reemerging Listeria monocytogenes Serovar 1/2a Strain in Central Italy. Microbiology Resource Announcements. 2018; 7 (23):e01069-18.

Chicago/Turabian Style

Massimiliano Orsini; Marina Torresi; Claudio Patavino; Patrizia Centorame; Antonio Rinaldi; Vicdalia Aniela Acciari; Gabriella Centorotola; Maurilia Marcacci; Massimo Ancora; Marco Di Domenico; Giuliana Blasi; Anna Duranti; Samuel Perticara; Adriano Di Pasquale; Cesare Cammà; Francesco Pomilio; Giacomo Migliorati. 2018. "Whole-Genome Sequence of a Reemerging Listeria monocytogenes Serovar 1/2a Strain in Central Italy." Microbiology Resource Announcements 7, no. 23: e01069-18.

Journal article
Published: 01 September 2018 in Journal of Medical Microbiology
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Purpose. From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidisciplinary network. Methodology. Epidemiological and microbiological surveillance was conducted to confirm the outbreak and to detect the food vehicle of infection. The origin and destination of the implicated food and its ingredients were investigated by tracing-back and -forward investigation. Results. Next-generation sequencing confirmed the unique outbreak strain. On 4 January 2016, a L. monocytogenes strain with pulsotype indistinguishable from that isolated from clinical cases in the outbreak was detected in a sample of hog head cheese purchased from a retail supermarket by one of the patients. The hog head cheese was produced by a small meat processing plant in the Marche region, where microbiological investigation confirmed environmental and food contamination by the outbreak strain. Plant production was suspended and all contaminated batches of the hog head cheese were withdrawn from the market by 19 February by local health authority. We subsequently observed a sharp decline in clinical cases, the last being reported on 11 March 2016. Conclusion. The key factor in the timely conclusion of this investigation was intersectoral collaboration among epidemiologists, microbiologists, veterinarians, statisticians and health and food safety authorities at national, regional and local levels.

ACS Style

Anna Duranti; Michela Sabbatucci; Giuliana Blasi; Vicdalia Aniela Acciari; Massimo Ancora; Antonino Bella; Luca Busani; Patrizia Centorame; Cesare Cammà; Fabrizio Conti; Dario De Medici; Marco Di Domenico; Violeta Di Marzio; Giovanni Filippini; Alfonsina Fiore; Stefano Fisichella; Antonietta Gattuso; Monica Gianfranceschi; Caterina Graziani; Fabrizia Guidi; Maurilia Marcacci; Cristina Marfoglia; Diana Neri; Massimiliano Orsini; Donatella Ottaviani; Annalisa Petruzzelli; Patrizio Pezzotti; Caterina Rizzo; Anna Ruolo; Gaia Scavia; Stefania Scuota; Giuliano Tagliavento; Alberto Tibaldi; Francesco Tonucci; Marina Torresi; Giacomo Migliorati; Francesco Pomilio. A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products. Journal of Medical Microbiology 2018, 67, 1351 -1360.

AMA Style

Anna Duranti, Michela Sabbatucci, Giuliana Blasi, Vicdalia Aniela Acciari, Massimo Ancora, Antonino Bella, Luca Busani, Patrizia Centorame, Cesare Cammà, Fabrizio Conti, Dario De Medici, Marco Di Domenico, Violeta Di Marzio, Giovanni Filippini, Alfonsina Fiore, Stefano Fisichella, Antonietta Gattuso, Monica Gianfranceschi, Caterina Graziani, Fabrizia Guidi, Maurilia Marcacci, Cristina Marfoglia, Diana Neri, Massimiliano Orsini, Donatella Ottaviani, Annalisa Petruzzelli, Patrizio Pezzotti, Caterina Rizzo, Anna Ruolo, Gaia Scavia, Stefania Scuota, Giuliano Tagliavento, Alberto Tibaldi, Francesco Tonucci, Marina Torresi, Giacomo Migliorati, Francesco Pomilio. A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products. Journal of Medical Microbiology. 2018; 67 (9):1351-1360.

Chicago/Turabian Style

Anna Duranti; Michela Sabbatucci; Giuliana Blasi; Vicdalia Aniela Acciari; Massimo Ancora; Antonino Bella; Luca Busani; Patrizia Centorame; Cesare Cammà; Fabrizio Conti; Dario De Medici; Marco Di Domenico; Violeta Di Marzio; Giovanni Filippini; Alfonsina Fiore; Stefano Fisichella; Antonietta Gattuso; Monica Gianfranceschi; Caterina Graziani; Fabrizia Guidi; Maurilia Marcacci; Cristina Marfoglia; Diana Neri; Massimiliano Orsini; Donatella Ottaviani; Annalisa Petruzzelli; Patrizio Pezzotti; Caterina Rizzo; Anna Ruolo; Gaia Scavia; Stefania Scuota; Giuliano Tagliavento; Alberto Tibaldi; Francesco Tonucci; Marina Torresi; Giacomo Migliorati; Francesco Pomilio. 2018. "A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products." Journal of Medical Microbiology 67, no. 9: 1351-1360.

Journal article
Published: 04 July 2018 in Journal of Virological Methods
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Prompt and accurate diagnosis is warranted for infectious diseases of domestic animals which may have a significant impact on animal production or clinical practice. In this study, the identification and genetic characterization of a bovine enterovirus (BEV) strain isolated from a calf with diarrhea, are described. Two different next generation sequencing platforms were employed. Shotgun metagenomic accomplished by MinION sequencing (Oxford Nanopore Technologies) allowed the identification of BEV RNA from a cell-culture isolate. BEV was then confirmed by a specific real time RT-PCR assay. To achieve the whole genome of this isolate, sequence reads obtained by MinION were coupled with those originating from NextSeq500 (Illumina). Genomic relatedness and phylogeny with extant BEV strains is also reported. Overall, this manuscript highlights the use of the portable MinION sequence technology as a tool for support diagnostics in veterinary practice.

ACS Style

Maria Serena Beato; Maurilia Marcacci; Eliana Schiavon; Luciano Bertocchi; Marco Di Domenico; Alessia Peserico; Monica Mion; Guendalina Zaccaria; Lara Cavicchio; Iolanda Mangone; Elena Soranzo; Claudio Patavino; Cesare Cammà; Alessio Lorusso. Identification and genetic characterization of bovine enterovirus by combination of two next generation sequencing platforms. Journal of Virological Methods 2018, 260, 21 -25.

AMA Style

Maria Serena Beato, Maurilia Marcacci, Eliana Schiavon, Luciano Bertocchi, Marco Di Domenico, Alessia Peserico, Monica Mion, Guendalina Zaccaria, Lara Cavicchio, Iolanda Mangone, Elena Soranzo, Claudio Patavino, Cesare Cammà, Alessio Lorusso. Identification and genetic characterization of bovine enterovirus by combination of two next generation sequencing platforms. Journal of Virological Methods. 2018; 260 ():21-25.

Chicago/Turabian Style

Maria Serena Beato; Maurilia Marcacci; Eliana Schiavon; Luciano Bertocchi; Marco Di Domenico; Alessia Peserico; Monica Mion; Guendalina Zaccaria; Lara Cavicchio; Iolanda Mangone; Elena Soranzo; Claudio Patavino; Cesare Cammà; Alessio Lorusso. 2018. "Identification and genetic characterization of bovine enterovirus by combination of two next generation sequencing platforms." Journal of Virological Methods 260, no. : 21-25.

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Published: 14 June 2018 in Genome Announcements
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We report the whole-genome sequences of two Listeria monocytogenes strains responsible for a severe invasive listeriosis outbreak in central Italy that occurred in 2015 and 2016. These two strains differ by a single band in their pulsed-field gel electrophoresis (PFGE) profiles.

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Massimiliano Orsini; Alessandra Cornacchia; Claudio Patavino; Marina Torresi; Patrizia Centorame; Vicdalia Aniela Acciari; Anna Ruolo; Maurilia Marcacci; Massimo Ancora; Marco Di Domenico; Iolanda Mangone; Giuliana Blasi; Anna Duranti; Cesare Cammà; Francesco Pomilio; Giacomo Migliorati. Whole-Genome Sequences of Two Listeria monocytogenes Serovar 1/2a Strains Responsible for a Severe Listeriosis Outbreak in Central Italy. Genome Announcements 2018, 6, e00236-18 .

AMA Style

Massimiliano Orsini, Alessandra Cornacchia, Claudio Patavino, Marina Torresi, Patrizia Centorame, Vicdalia Aniela Acciari, Anna Ruolo, Maurilia Marcacci, Massimo Ancora, Marco Di Domenico, Iolanda Mangone, Giuliana Blasi, Anna Duranti, Cesare Cammà, Francesco Pomilio, Giacomo Migliorati. Whole-Genome Sequences of Two Listeria monocytogenes Serovar 1/2a Strains Responsible for a Severe Listeriosis Outbreak in Central Italy. Genome Announcements. 2018; 6 (24):e00236-18.

Chicago/Turabian Style

Massimiliano Orsini; Alessandra Cornacchia; Claudio Patavino; Marina Torresi; Patrizia Centorame; Vicdalia Aniela Acciari; Anna Ruolo; Maurilia Marcacci; Massimo Ancora; Marco Di Domenico; Iolanda Mangone; Giuliana Blasi; Anna Duranti; Cesare Cammà; Francesco Pomilio; Giacomo Migliorati. 2018. "Whole-Genome Sequences of Two Listeria monocytogenes Serovar 1/2a Strains Responsible for a Severe Listeriosis Outbreak in Central Italy." Genome Announcements 6, no. 24: e00236-18.

Journal article
Published: 01 April 2018 in Infection, Genetics and Evolution
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Bluetongue (BT), is one of the OIE-listed major diseases of ruminants. Following the official report of BT virus serotype 3 (BTV-3) in a sheep in Cap Bon (Tunisia), blood and serum samples of ruminants were collected from some areas of Tunisia to further investigate the presence of this virus in the country. To this aim, a specific quantitative real time RT-PCR has been first developed for the detection and quantitation of BTV-3 RNA from field specimens. Out of 62 collected blood samples, 23 were shown to be positive for BTV-3 RNA. Isolation on cell cultures was also possible from six blood samples. Whole genome sequencing by NGS and partial Seg-2 sequencing revealed the circulation of two different western strains of BTV-3, one circulating in Cap Bon and neighboring areas, and the other circulating nearby the border with Libya. The presence of a putative novel BTV serotype (BTV-Y TUN2017) in sheep introduced from Libya to Tunisia, genomically related to the BTV strain contaminating a commercially-available sheep pox vaccine and to BTV-26, has been also demonstrated. Natural co-infection of BTV-3 and BTV-Y TUN2017 has been confirmed in one sheep. Overall, we demonstrated the circulation of two different BTV-3 strains in Tunisia. This finding highlights the pressing need for a prompt production and release of a novel inactivated BTV-3 vaccine to be used in case of emergence or proactively in the areas of Southern Europe at major risk of BTV introduction. The assessment of a novel vaccine will certainly exalt the role and importance of surveillance activities and collaboration with Northern African countries.

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Alessio Lorusso; Soufien Sghaier; Marco Di Domenico; Mohamed Elias Barbria; Guendalina Zaccaria; Aida Megdich; Ottavio Portanti; Imed Ben Seliman; Massimo Spedicato; Federica Pizzurro; Irene Carmine; Liana Teodori; Mejdi Mahjoub; Iolanda Mangone; Alessandra Leone; Salah Hammami; Maurilia Marcacci; Giovanni Savini. Analysis of bluetongue serotype 3 spread in Tunisia and discovery of a novel strain related to the bluetongue virus isolated from a commercial sheep pox vaccine. Infection, Genetics and Evolution 2018, 59, 63 -71.

AMA Style

Alessio Lorusso, Soufien Sghaier, Marco Di Domenico, Mohamed Elias Barbria, Guendalina Zaccaria, Aida Megdich, Ottavio Portanti, Imed Ben Seliman, Massimo Spedicato, Federica Pizzurro, Irene Carmine, Liana Teodori, Mejdi Mahjoub, Iolanda Mangone, Alessandra Leone, Salah Hammami, Maurilia Marcacci, Giovanni Savini. Analysis of bluetongue serotype 3 spread in Tunisia and discovery of a novel strain related to the bluetongue virus isolated from a commercial sheep pox vaccine. Infection, Genetics and Evolution. 2018; 59 ():63-71.

Chicago/Turabian Style

Alessio Lorusso; Soufien Sghaier; Marco Di Domenico; Mohamed Elias Barbria; Guendalina Zaccaria; Aida Megdich; Ottavio Portanti; Imed Ben Seliman; Massimo Spedicato; Federica Pizzurro; Irene Carmine; Liana Teodori; Mejdi Mahjoub; Iolanda Mangone; Alessandra Leone; Salah Hammami; Maurilia Marcacci; Giovanni Savini. 2018. "Analysis of bluetongue serotype 3 spread in Tunisia and discovery of a novel strain related to the bluetongue virus isolated from a commercial sheep pox vaccine." Infection, Genetics and Evolution 59, no. : 63-71.

Journal article
Published: 02 February 2018 in Transboundary and Emerging Diseases
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In this rapid communication, a novel atypical bluetongue virus (BTV) strain detected in goats in the Piedmont region (north-western Italy) is described. This strain, BTV-Z ITA2017, is most related in Seg-2/VP-2 (83.8% nt/82.7% aa) to strain TOV of BTV-25. Reactive antisera of goats positive by cELISA for BTV antibodies failed to neutralize a chimeric virus expressing the outermost protein of TOV. Infected animals displayed low levels of RNAemia and absence of clinical signs consistent with bluetongue infection, a scenario described in animals infected with atypical BTV strains.

ACS Style

Maurilia Marcacci; Serena Sant; Iolanda Mangone; Maria Goria; Alessandro Dondo; Simona Zoppi; Rene van Gennip; Maria Cristina Radaelli; Cesare Cammà; Piet A. van Rijn; Giovanni Savini; Alessio Lorusso. One after the other: A novel Bluetongue virus strain related to Toggenburg virus detected in the Piedmont region (North-western Italy), extends the panel of novel atypical BTV strains. Transboundary and Emerging Diseases 2018, 65, 370 -374.

AMA Style

Maurilia Marcacci, Serena Sant, Iolanda Mangone, Maria Goria, Alessandro Dondo, Simona Zoppi, Rene van Gennip, Maria Cristina Radaelli, Cesare Cammà, Piet A. van Rijn, Giovanni Savini, Alessio Lorusso. One after the other: A novel Bluetongue virus strain related to Toggenburg virus detected in the Piedmont region (North-western Italy), extends the panel of novel atypical BTV strains. Transboundary and Emerging Diseases. 2018; 65 (2):370-374.

Chicago/Turabian Style

Maurilia Marcacci; Serena Sant; Iolanda Mangone; Maria Goria; Alessandro Dondo; Simona Zoppi; Rene van Gennip; Maria Cristina Radaelli; Cesare Cammà; Piet A. van Rijn; Giovanni Savini; Alessio Lorusso. 2018. "One after the other: A novel Bluetongue virus strain related to Toggenburg virus detected in the Piedmont region (North-western Italy), extends the panel of novel atypical BTV strains." Transboundary and Emerging Diseases 65, no. 2: 370-374.

Article
Published: 18 December 2017 in Veterinary Medicine and Science
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Here we report and characterize a porcine epidemic diarrhea (PED) outbreak which occurred in a swine fattening farm in the province of Teramo, Abruzzi region (central Italy), in January 2016. PED virus (PEDV) identification was determined by real‐time RT‐PCR performed on RNAs purified from fecal samples collected from two symptomatic pigs. Whole genome sequence (PEDV 1842/2016) was also obtained by next generation sequencing straight from RNA purified from one fecal sample. Genome comparison with extant global PEDV strains revealed a high nucleotide identity with recently reported European and American S‐INDEL PEDVs. Efficient sequencing, share of genomic data combined with the implementation of epidemiological tools would be the ideal approach for study and analysis of transboundary infectious diseases as PED.

ACS Style

Federica Pizzurro; Francesca Cito; Guendalina Zaccaria; Massimo Spedicato; Angelo Cerella; Massimiliano Orsini; Mario Forzan; Nicola D'Alterio; Alessio Lorusso; Maurilia Marcacci. Outbreak of porcine epidemic diarrhoea virus (PEDV) in Abruzzi region, central‐Italy. Veterinary Medicine and Science 2017, 4, 73 -79.

AMA Style

Federica Pizzurro, Francesca Cito, Guendalina Zaccaria, Massimo Spedicato, Angelo Cerella, Massimiliano Orsini, Mario Forzan, Nicola D'Alterio, Alessio Lorusso, Maurilia Marcacci. Outbreak of porcine epidemic diarrhoea virus (PEDV) in Abruzzi region, central‐Italy. Veterinary Medicine and Science. 2017; 4 (2):73-79.

Chicago/Turabian Style

Federica Pizzurro; Francesca Cito; Guendalina Zaccaria; Massimo Spedicato; Angelo Cerella; Massimiliano Orsini; Mario Forzan; Nicola D'Alterio; Alessio Lorusso; Maurilia Marcacci. 2017. "Outbreak of porcine epidemic diarrhoea virus (PEDV) in Abruzzi region, central‐Italy." Veterinary Medicine and Science 4, no. 2: 73-79.

Journal article
Published: 01 July 2017 in Infection, Genetics and Evolution
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In recent years, novel Bluetongue virus (BTV) serotypes have been isolated and/or sequenced by researchers within the field. During Bluetongue surveillance activities, we identified a putative novel BTV serotype in healthy goats from Sardinia, Italy. RNAs purified from blood and serum samples were positive for BTV by a generic real time RT-PCR and c-ELISA, respectively, whereas genotyping and serotyping were unsuccessful. By NGS, the whole genome sequence was obtained from two blood samples (BTV-X ITL2015 strains 34200 and 33531). Overall, Seg 2 of BTV-X ITL2015 shows the highest identity (75.3-75.5% nt/77.4-78.1% aa) with recently isolated BTV-27s from Corsica and with the last discovered BTV XJ1407 from China (75.9% nt /78.2% aa), whereas it is less related with BTV-25 from Switzerland (73.0% nt/75.0% aa) and BTV-26 from Kuwait (62.0% nt/60.5% aa). A specific RT-qPCR targeting Seg 2 of BTV-X ITL2015 was assessed in this study. Considering the Seg 2/VP2 identity of BTV-X ITL2015 with BTV-25, 26, 27s and BTV XJ1407 and that serum of BTV-X ITL2015 infected goats failed to neutralize all tested extant serotypes, we propose the existence of a novel BTV serotype circulating in goats in Sardinia. Isolation was so far unsuccessful thus hampering proper antigenic characterization.

ACS Style

Giovanni Savini; Giantonella Puggioni; Giorgio Meloni; Maurilia Marcacci; Marco Di Domenico; Angela Maria Rocchigiani; Massimo Spedicato; Annalisa Oggiano; Daniela Manunta; Liana Teodori; Alessandra Leone; Ottavio Portanti; Francesca Cito; Annamaria Conte; Massimiliano Orsini; Cesare Cammà; Paolo Calistri; Armando Giovannini; Alessio Lorusso. Novel putative Bluetongue virus in healthy goats from Sardinia, Italy. Infection, Genetics and Evolution 2017, 51, 108 -117.

AMA Style

Giovanni Savini, Giantonella Puggioni, Giorgio Meloni, Maurilia Marcacci, Marco Di Domenico, Angela Maria Rocchigiani, Massimo Spedicato, Annalisa Oggiano, Daniela Manunta, Liana Teodori, Alessandra Leone, Ottavio Portanti, Francesca Cito, Annamaria Conte, Massimiliano Orsini, Cesare Cammà, Paolo Calistri, Armando Giovannini, Alessio Lorusso. Novel putative Bluetongue virus in healthy goats from Sardinia, Italy. Infection, Genetics and Evolution. 2017; 51 ():108-117.

Chicago/Turabian Style

Giovanni Savini; Giantonella Puggioni; Giorgio Meloni; Maurilia Marcacci; Marco Di Domenico; Angela Maria Rocchigiani; Massimo Spedicato; Annalisa Oggiano; Daniela Manunta; Liana Teodori; Alessandra Leone; Ottavio Portanti; Francesca Cito; Annamaria Conte; Massimiliano Orsini; Cesare Cammà; Paolo Calistri; Armando Giovannini; Alessio Lorusso. 2017. "Novel putative Bluetongue virus in healthy goats from Sardinia, Italy." Infection, Genetics and Evolution 51, no. : 108-117.

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Published: 20 April 2017 in Genome Announcements
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Canine adenovirus type 1 (CAdV-1), a DNA virus of the family Adenoviridae , causes infectious canine hepatitis, a highly contagious disease primarily affecting canids. In this report, we describe the isolation and whole-genome sequence of a CAdV-1 isolate from the liver of a free-ranging wolf ( Canis lupus ).

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Federica Pizzurro; Maurilia Marcacci; Guendalina Zaccaria; Massimiliano Orsini; Francesca Cito; Alfonso Rosamilia; Ludovica Di Renzo; Daniela Malatesta; Daria Di Sabatino; Alessio Lorusso. Genome Sequence of Canine Adenovirus Type 1 Isolated from a Wolf ( Canis lupus ) in Southern Italy. Genome Announcements 2017, 5, e00225-17 .

AMA Style

Federica Pizzurro, Maurilia Marcacci, Guendalina Zaccaria, Massimiliano Orsini, Francesca Cito, Alfonso Rosamilia, Ludovica Di Renzo, Daniela Malatesta, Daria Di Sabatino, Alessio Lorusso. Genome Sequence of Canine Adenovirus Type 1 Isolated from a Wolf ( Canis lupus ) in Southern Italy. Genome Announcements. 2017; 5 (16):e00225-17.

Chicago/Turabian Style

Federica Pizzurro; Maurilia Marcacci; Guendalina Zaccaria; Massimiliano Orsini; Francesca Cito; Alfonso Rosamilia; Ludovica Di Renzo; Daniela Malatesta; Daria Di Sabatino; Alessio Lorusso. 2017. "Genome Sequence of Canine Adenovirus Type 1 Isolated from a Wolf ( Canis lupus ) in Southern Italy." Genome Announcements 5, no. 16: e00225-17.

Journal article
Published: 15 March 2017 in Transboundary and Emerging Diseases
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Since 1998, southern Europe has experienced multiple incursions of different serotypes and topotypes of Bluetongue virus, a vector-borne transmitted virus, the causative agent of Bluetongue (BT), a major disease of ruminants. Some of these incursions originated from northern Africa, likely because of wind-blown dissemination of infected midges. In this report, we describe the detection and whole genome characterization of a novel BTV-3 strain identified in a symptomatic sheep in Tunisia. Sequences were immediately deposited with the GenBank Database under Accession Nos KY432369-KY432378. Alert and preparedness are requested to face the next vector seasons in northern Africa and the potential incursion of this novel strain in southern Europe.

ACS Style

Soufien Sghaier; A. Lorusso; Ottavio Portanti; Maurilia Marcacci; M. Orsini; M. E. Barbria; A. S. Mahmoud; Salah Hammami; Antonio Petrini; Giovanni Savini. A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016. Transboundary and Emerging Diseases 2017, 102, 12891 -715.

AMA Style

Soufien Sghaier, A. Lorusso, Ottavio Portanti, Maurilia Marcacci, M. Orsini, M. E. Barbria, A. S. Mahmoud, Salah Hammami, Antonio Petrini, Giovanni Savini. A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016. Transboundary and Emerging Diseases. 2017; 102 (3):12891-715.

Chicago/Turabian Style

Soufien Sghaier; A. Lorusso; Ottavio Portanti; Maurilia Marcacci; M. Orsini; M. E. Barbria; A. S. Mahmoud; Salah Hammami; Antonio Petrini; Giovanni Savini. 2017. "A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016." Transboundary and Emerging Diseases 102, no. 3: 12891-715.