Enzymes have been recognized as highly efficient biocatalysts, whereas characteristics such as poor stability and single reaction type greatly significantly limit their wide application. Hence, the exploitation of suitable carriers for immobilized enzymes enables the provision of a protective layer for the enzyme, with the capability of chemical and biological cascade catalysis. Among the various immobilization carriers, metal‐organic frameworks (MOFs), covalent organic frameworks (COFs) and hydrogen‐bonded organic frameworks (HOFs) have been emerging as a promising strategy to surpass the inherent instability and other limitations of free enzymes. Specifically, the integration of such artificial porous materials as carriers improves the stability and reusability of enzymes, while simultaneously affording a platform for multifunctional applications. Herein, this review systematically discusses the various preparation strategies and advantages of artificial porous materials, while elucidating the effects of different immobilization methods on enzyme activity. Furthermore, the innovative applications of artificial porous materials as multifunctional carriers in the field of enzyme immobilization fields such as enzyme carriers, photocatalysts, chemical catalysts and sensing are also comprehensively summarized here, thus demonstrating their multifunctional characteristics and promising applications in addressing complex biotransformation challenges.

Aspergoterpenins A–D (1–4), four new bisabolane sesquiterpenoid derivatives, were obtained from the endophytic fungus, Aspergillus versicolor, together with eight known compounds (5–1 2), and their structures were elucidated by a comprehensive analysis of their NMR (Nuclear Magnetic Resonance), MS (Mass Spectrum) and CD (Circular Dichroism) spectra. Aspergoterpenin A (1) was the first example with a characteristic ketal bridged-ring part in the degraded natural bisabolane-type sesquiterpene structures. The compounds 1–1 2 displayed no significant activities against four cancer cell lines (A549, Caski, HepG2 and MCF-7). Further, the antimicrobial activities to Erwinia carotovora sub sp. Carotovora were evaluated, and the results showed that compounds 1–1 2 displayed antimicrobial activities with MIC values ranging from 15.2 to 85.2 μg/mL.

Seeking a strategy for triggering the cryptic natural product biosynthesis to yield novel compounds in the plant-associated fungus Xylaria sp., the effect of culture conditions on metabolite production was investigated. A shift in the production of five known cytochalasin-type analogues 1–5 to six new α-pyrone derivatives, xylapyrones A–F (compounds 6–11), from a solid to a liquid medium was observed. These compounds were identified by analysis of 1D and 2D NMR and HRMS data. Compounds 1–3 showed moderate cytotoxicity against HepG2 and Caski cancer cell lines with IC₅₀ values ranging from 25 to 63 μM and compounds 4–11 were found to be inactive, with IC₅₀ values >100 μM.