Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated histone tails that consequently interact more closely with DNA, leading to chromatin state refractory to transcription. Zea mays HDA108 belongs to the Rpd3/HDA1 HDAC family and is ubiquitously expressed during development. The newly isolated hda108/hda108 insertional mutant exhibited many developmental defects: significant reduction in plant height, alterations of shoot and leaf development, and alterations of inflorescence patterning and fertility. Western blot analyses and immunolocalization experiments revealed an evident increase in histone acetylation, accompanied by a marked reduction in H3K9 dimethylation, in mutant nuclei. The DNA methylation status, in the CHG sequence context, and the transcript level of ribosomal sequences were also affected in hda108 mutants, while enrichment in H3 and H4 acetylation characterizes both repetitive and nonrepetitive transcriptional up-regulated loci. RNA-Seq of both young leaf and anthers indicated that transcription factor expression is highly affected and that the pollen developmental program is disrupted in hda108 mutants. Crosses between hda108/hda108 and epiregulator mutants did not produce any double mutant progeny indicating possible genetic interactions of HDA108 with distinct epigenetic pathways. Our findings indicate that HDA108 is directly involved in regulation of maize development, fertility, and epigenetic regulation of genome activity.

The optimization of irrigation use in agriculture is a key challenge to increase farm profitability and reduce its ecological footprint. To this context, an understanding of more efficient irrigation systems includes the assessment of water redistribution at the microscale. This study aimed to investigate rainfall interception by maize canopy and to model the soil water dynamics at row scale as a result of rain and sprinkler irrigation with HYDRUS 2D/3D. On average, 78% of rainfall below the maize canopy was intercepted by the leaves and transferred along the stem (stemflow), while only 22% reached the ground directly (throughfall). In addition, redistribution of the water with respect to the amount (both rain and irrigation) showed that the stemflow/throughfall ratio decreased logarithmically at increasing values of incident rainfall, suggesting the plant capacity to confine the water close to the roots and diminish water stress conditions. This was also underlined by higher soil moisture values observed in the row than in the inter-row at decreasing rainfall events. Modelled data highlighted different behavior in terms of soil water dynamics between simulated irrigation water distributions, although they did not show significant changes in terms of crop water use efficiency. These results were most likely affected by the soil type (silty-loam) where the experiment was conducted, as it had unfavorable physical conditions for the rapid vertical water movement that would have increased infiltration and drainage.