The purpose of this study was to verify the correlation between the activity of urease and the content of nickel in soil of temperate climate in relation to the land management. Moreover, the metal speciation was taken into account in order to search for the above-mentioned correlation. Arable lands, forested lands, and wastelands were analyzed. The basic soil parameters were determined such as pH, clay fraction content, and organic matter content. The speciation of nickel was studied by using BCR (Bureau Communitaire de Reference) sequential extraction procedure and flame atomic absorption spectrometry, while the urease activity was determined spectrophotometrically. The pseudo-total content of Ni in every sample was below 30 mg kg⁻¹of dry soil. The dominant form of nickel in the soil samples was the residual form. Although the urease activity varied slightly between the samples, the differences turned out to be statistically insignificant. However, the highly positive correlations between the urease activity, organic matter content, and the pseudo-total content of Ni were found and discussed. Moreover, the positive correlations between the urease activity and two geochemical forms of nickel, namely,activeandresidualform, were confirmed. The results of performed experiments prove that the method of land management does not significantly affect either the topsoil urease activity or the nickel distribution in the case of the extensive agriculture and forestry. However, the existence of at least two forms of the active soil urease was proposed. The first one―contained in the soil solution or loosely adsorbed on the soil particulates and the second one―strongly adsorbed onto the clay minerals. Also the complex role of the organic matter in protecting urease from external factors was presented. Finally, it was postulated that the nickel content in soil may be the indicator of the soil urease activity.