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Scrapie is considered an endemic disease in both sheep and goats in Greece. However, contrary to sheep, in goats more than one prion protein (PrP) polymorphism has been recognized as a candidate for resistance breeding against the disease. For an impression, candidates which are circulating, (i) brain samples (n = 525) from scrapie-affected (n = 282) and non-affected (n = 243) animals within the national surveillance program, and (ii) individual blood samples (n = 1708) from affected (n = 241) and non-affected (n = 1467) herds, in a large part of mainland Greece and its islands, were collected and assayed. A dedicated Taqman method was used to test for amino acid polymorphisms 110T/P, 146N/S/D, 211R/Q, and 222Q/K. Highly prevalent genotypes were 110TT, 146NN, 211RR, and 222QQ. The frequencies of polymorphisms in blood and negative brain samples for codons 110P, 211Q, and 222K were 4.0%, 3.0%, and 1.9%, respectively, while 146D (0.7%) was present only on Karpathos island. Codon 110P was exclusively found in scrapie-negative brains, and homozygous 110P/P in two scrapie-negative goats. It is concluded that breeding programs in Karpathos could focus on codon 146D, while in other regions carriers of the 110P and 222K allele should be sought. Case-control and challenge studies are now necessary to elucidate the most efficient breeding strategies.
Athanasios Gelasakis; Evridiki Boukouvala; Maria Babetsa; Efstathios Katharopoulos; Vayia Palaska; Dimitra Papakostaki; Nektarios Giadinis; Dimitrios Loukovitis; Jan Langeveld; Loukia Ekateriniadou. Polymorphisms of Codons 110, 146, 211 and 222 at the Goat PRNP Locus and Their Association with Scrapie in Greece. Animals 2021, 11, 2340 .
AMA StyleAthanasios Gelasakis, Evridiki Boukouvala, Maria Babetsa, Efstathios Katharopoulos, Vayia Palaska, Dimitra Papakostaki, Nektarios Giadinis, Dimitrios Loukovitis, Jan Langeveld, Loukia Ekateriniadou. Polymorphisms of Codons 110, 146, 211 and 222 at the Goat PRNP Locus and Their Association with Scrapie in Greece. Animals. 2021; 11 (8):2340.
Chicago/Turabian StyleAthanasios Gelasakis; Evridiki Boukouvala; Maria Babetsa; Efstathios Katharopoulos; Vayia Palaska; Dimitra Papakostaki; Nektarios Giadinis; Dimitrios Loukovitis; Jan Langeveld; Loukia Ekateriniadou. 2021. "Polymorphisms of Codons 110, 146, 211 and 222 at the Goat PRNP Locus and Their Association with Scrapie in Greece." Animals 11, no. 8: 2340.
The European seabass is one of the main commercial fish produced in Mediterranean marine aquaculture. Recently, its production has been negatively affected by losses due to frequent and recurring outbreaks of parasitic, bacterial and viral diseases. In recent years, the gill parasites Diplectanum aequans and Lernanthropus kroyeri are increasingly becoming more dominant and contributing more significantly to the observed losses. Genetic improvement for disease resistance represents an important strategy for controlling infectious diseases in farmed fish by increasing their robustness. In order to determine the possibility of including such trait in selective breeding programs, we need to comprehend whether additive genetic variation for resistance against Diplectanum aequans and Lernanthropus kroyeri exists. For this purpose, two open-sea parasite cohabitation trials (for two consecutive years) were performed in the commercial production sites of a private company (Nireus S.A), that had high infestation with Diplectanum aequans and Lernanthropus kroyeri. Juvenile European seabass (9425 offspring from 91 full-sib and half-sib families per year), originating from the company's breeding program were equally divided into two groups and transferred to two commercial farming sites located in the areas of Nafpactos and Sagiada, in western Greece, for the intended cohabitation studies. The parasite numbers on all the gill arches were counted and recorded at the end of the trials for the infestation levels. A third site (Palairos) without any parasite infestation was used as a control site. A multi-trait animal model was used to estimate the variance-covariance components and to evaluate the genetic parameters for Parasite Counts, recorded from all the gill arches of the fish, and their corresponding growth in sea cages. The estimated heritabilities for parasite count, using untransformed data, were 0.20 (D. aequans) and 0.28 (L. kroyeri) and for transformed data 0.29 and 0.26, respectively. The heritability estimates for body weight were 0.42−0.51 for D. aequans and 0.28−0.51 for L. kroyeri trials. Similarly, estimated heritabilities for the growth in sea cages were 0.43 and 0.29, respectively. Although parasite count has a low to medium unfavorable genetic correlation with body weight and growth (0.09−0.37), it seems that it is not significantly impairing selection for growth. Furthermore, the results of this study are very promising in terms of the existing potential for genetic improvement of parasite resistance, and provides a good basis for further genetic analysis using molecular markers.
Maria Papapetrou; Zoi Kazlari; Kantham Papanna; Leonidas Papaharisis; Stavroula Oikonomou; Tereza Manousaki; Dimitrios Loukovitis; Lefteris Kottaras; Arkadios Dimitroglou; Evgenia Gourzioti; Charalampos Pagonis; Andreas Kostandis; Costas S. Tsigenopoulos; Dimitrios Chatziplis. On the trail of detecting genetic (co)variation between resistance to parasite infections (Diplectanum aequans and Lernanthropus kroyeri) and growth in European seabass (Dicentrarchus labrax). Aquaculture Reports 2021, 20, 100767 .
AMA StyleMaria Papapetrou, Zoi Kazlari, Kantham Papanna, Leonidas Papaharisis, Stavroula Oikonomou, Tereza Manousaki, Dimitrios Loukovitis, Lefteris Kottaras, Arkadios Dimitroglou, Evgenia Gourzioti, Charalampos Pagonis, Andreas Kostandis, Costas S. Tsigenopoulos, Dimitrios Chatziplis. On the trail of detecting genetic (co)variation between resistance to parasite infections (Diplectanum aequans and Lernanthropus kroyeri) and growth in European seabass (Dicentrarchus labrax). Aquaculture Reports. 2021; 20 ():100767.
Chicago/Turabian StyleMaria Papapetrou; Zoi Kazlari; Kantham Papanna; Leonidas Papaharisis; Stavroula Oikonomou; Tereza Manousaki; Dimitrios Loukovitis; Lefteris Kottaras; Arkadios Dimitroglou; Evgenia Gourzioti; Charalampos Pagonis; Andreas Kostandis; Costas S. Tsigenopoulos; Dimitrios Chatziplis. 2021. "On the trail of detecting genetic (co)variation between resistance to parasite infections (Diplectanum aequans and Lernanthropus kroyeri) and growth in European seabass (Dicentrarchus labrax)." Aquaculture Reports 20, no. : 100767.
There is a growing interest in selective breeding in European sea bass (Dicentrarchus labrax), especially regarding family selection based on growth performance. In particular, quantitative trait loci (QTL) identification in sea bass enhances the application of marker-assisted breeding for the genetic improvement of the production traits. The aims of the study were to identify potential QTL affecting stress and immunological indicators, body weight, and mortality after vibriosis injection in sea bass as well as to estimate heritability and genetic/phenotypic correlations for the aforementioned traits. To this end, stress test was performed on 960 offspring and a sub-group of them (420) was selected to explore the mortality after vibrio injection. Selective genotyping was performed in 620 offspring for 35 microsatellite markers and distributed into 6 linkage groups. The length of the genetic linkage map was 283.6 cM and the mean distance between the markers was 8.1 cM. QTL affecting body weight in three different growth periods detected on linkage groups LG1, LG4, LG6, and LG14. A QTL associated with weight in early growth stages (290–306 days post-hatching) was also identified on LG3. QTL analysis confirmed the existence of QTL affecting cortisol levels, on LG3 and LG14. Moreover, new QTL affecting only cortisol and glucose levels were detected on LG1 and LG23. No QTL affecting hormonal or biochemical marks was found on LG4 and LG6. Heritability of cortisol, lysozyme levels, and mortality were high (0.36, 0.55, and 0.38, respectively).
Dimitrios Chatziplis; Stavroula Oikonomou; Dimitrios Loukovitis; Dimitrios Tsiokos; Athanasios Samaras; Arkadios Dimitroglou; Lefteris Kottaras; Kantham Papanna; Leonidas Papaharisis; Costas Tsigenopoulos; Michail Pavlidis. QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus Labrax L. Animals 2020, 10, 1668 .
AMA StyleDimitrios Chatziplis, Stavroula Oikonomou, Dimitrios Loukovitis, Dimitrios Tsiokos, Athanasios Samaras, Arkadios Dimitroglou, Lefteris Kottaras, Kantham Papanna, Leonidas Papaharisis, Costas Tsigenopoulos, Michail Pavlidis. QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus Labrax L. Animals. 2020; 10 (9):1668.
Chicago/Turabian StyleDimitrios Chatziplis; Stavroula Oikonomou; Dimitrios Loukovitis; Dimitrios Tsiokos; Athanasios Samaras; Arkadios Dimitroglou; Lefteris Kottaras; Kantham Papanna; Leonidas Papaharisis; Costas Tsigenopoulos; Michail Pavlidis. 2020. "QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus Labrax L." Animals 10, no. 9: 1668.
The aim of this study was to estimate the genetic diversity of Greek and Bulgarian grapevine varieties with the use of microsatellite markers. The studied samples were collected from various productive vineyards, consisting of eight Greek and nine Bulgarian native varieties. In order to create a genetic profile for each sample, a multiplex PCR reaction method was used amplifying simultaneously seven microsatellite loci. Statistical analysis of data showed that there was a high degree of genetic heterogeneity among most of the varieties studied, highlighting the discriminative power of the chosen set of markers. Moreover, the synonymy of (I) Greek Pamid and Bulgarian Pamid and (II) Greek Zoumiatiko and Bulgarian Dimyat was suggested, as each variety pair had identical allele profiles in all loci examined. Regarding the Greek Mavrud and Bulgarian Mavrud varieties, there was a close genetic relationship between them, however, they did not share common alleles in all microsatellite loci and, therefore, should not be characterized as synonyms. On the other hand, Greek and Bulgarian Keratsouda, which were supposed to be common varieties, were found to be genetically different, supporting that these two varieties should be considered as homonyms. Despite the genotypic assay performed herein, we believe that additional molecular work is needed for the efficient management of Greek and Bulgarian grapevine genepools, as well as to safely suggest any synonym or homonym annotation.
Maria Papapetrou; Dimitrios Loukovitis; Orestis Papadopoulos; Zoi Kazlari; Anastasia Peristeraki; Slavina Arsenova; Desislava Bardarova; Desislava Doncheva; Serafeim Theocharis; Constantinos Karagiannidis; Stefanos Koundouras; Anastasia Giannakoula; Stamatis Aggelopoulos; Dimitrios Chatziplis. Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties. Diversity 2020, 12, 273 .
AMA StyleMaria Papapetrou, Dimitrios Loukovitis, Orestis Papadopoulos, Zoi Kazlari, Anastasia Peristeraki, Slavina Arsenova, Desislava Bardarova, Desislava Doncheva, Serafeim Theocharis, Constantinos Karagiannidis, Stefanos Koundouras, Anastasia Giannakoula, Stamatis Aggelopoulos, Dimitrios Chatziplis. Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties. Diversity. 2020; 12 (7):273.
Chicago/Turabian StyleMaria Papapetrou; Dimitrios Loukovitis; Orestis Papadopoulos; Zoi Kazlari; Anastasia Peristeraki; Slavina Arsenova; Desislava Bardarova; Desislava Doncheva; Serafeim Theocharis; Constantinos Karagiannidis; Stefanos Koundouras; Anastasia Giannakoula; Stamatis Aggelopoulos; Dimitrios Chatziplis. 2020. "Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties." Diversity 12, no. 7: 273.
This paper is intended to review the current practices and challenges regarding the corrosion of the Greek sewer systems with an emphasis on biocorrosion and to provide recommendations to avoid it. The authors followed a holistic approach, which included survey data obtained by local authorities serving more than 50% of the total country’s population and validated the survey answers with field measurements and analyses. The exact nature and extent of concrete biocorrosion problems in Greece are presented for the first time. Moreover, the overall condition of the sewer network, the maintenance frequency, and the corrosion prevention techniques used in Greece are also presented. Results from field measurements showed the existence of H2S in the gaseous phase (i.e., precursor of the H2SO4 formation in the sewer) and acidithiobacillus bacteria (i.e., biocorrosion causative agent) in the slime, which exists at the interlayer between the concrete wall and the sewage. Biocorrosion seems to mainly affect old concrete networks, and the replacement of the destroyed concrete pipes with new polyvinyl chloride (PVC) ones is currently common practice. However, in most cases, the replacement cost is high, and the authors provide some recommendations to increase the current service life of concrete pipes.
Georgios Fytianos; Vasilis Baltikas; Dimitrios Loukovitis; Dimitra Banti; Athanasios Sfikas; Efthimios Papastergiadis; Petros Samaras. Biocorrosion of Concrete Sewers in Greece: Current Practices and Challenges. Sustainability 2020, 12, 2638 .
AMA StyleGeorgios Fytianos, Vasilis Baltikas, Dimitrios Loukovitis, Dimitra Banti, Athanasios Sfikas, Efthimios Papastergiadis, Petros Samaras. Biocorrosion of Concrete Sewers in Greece: Current Practices and Challenges. Sustainability. 2020; 12 (7):2638.
Chicago/Turabian StyleGeorgios Fytianos; Vasilis Baltikas; Dimitrios Loukovitis; Dimitra Banti; Athanasios Sfikas; Efthimios Papastergiadis; Petros Samaras. 2020. "Biocorrosion of Concrete Sewers in Greece: Current Practices and Challenges." Sustainability 12, no. 7: 2638.
Aim: To examine the association between ApoE genetic polymorphisms and BMI.Methods: This case control study of randomly selected, free living individuals from North Greece investigated whether traditional Greek Orthodox dietary practices could affect obesity markers independent of genetic influences, by examining the Waist circumference (WC), waist hip ratio (WHR) and % fat mass (% FM), were measured in healthy adults who follow the rules of Greek Orthodox fasting compared to those who did not. 382 subjects (246 women and 136 men) were included in the analysis, 161 fasters and 220 non-fasters as a control group. Results: Age affected obesity markers in all participants with more obesity in the older subjects. ApoE alleles did not differ between fasting and controls. When fasters where classified as obese and non-obese, there was no association with age (p>0,077). In the control group, BMI and WC were associated with age and gender and WHR with apoE alleles (p
Alexandra A Koulouri; Sousana K Papadopoulou; Dimitrios Loukovitis; Nikolaos Rodopaios; Eleni Vasara; Maria Hassapidou; Fani Biskanaki; Dimitrios Tasoulas; Elias Tassoulas; Andrew Coats; Anthony Kafatos. Association Between ApoE Polymorphism in Obesity Markers in Healthy Adults Who Follow the Greek Orthodox Fasting Rules. International Cardiovascular Forum Journal 2019, 15, 1 .
AMA StyleAlexandra A Koulouri, Sousana K Papadopoulou, Dimitrios Loukovitis, Nikolaos Rodopaios, Eleni Vasara, Maria Hassapidou, Fani Biskanaki, Dimitrios Tasoulas, Elias Tassoulas, Andrew Coats, Anthony Kafatos. Association Between ApoE Polymorphism in Obesity Markers in Healthy Adults Who Follow the Greek Orthodox Fasting Rules. International Cardiovascular Forum Journal. 2019; 15 ():1.
Chicago/Turabian StyleAlexandra A Koulouri; Sousana K Papadopoulou; Dimitrios Loukovitis; Nikolaos Rodopaios; Eleni Vasara; Maria Hassapidou; Fani Biskanaki; Dimitrios Tasoulas; Elias Tassoulas; Andrew Coats; Anthony Kafatos. 2019. "Association Between ApoE Polymorphism in Obesity Markers in Healthy Adults Who Follow the Greek Orthodox Fasting Rules." International Cardiovascular Forum Journal 15, no. : 1.
Food authenticity has received an increasing focus due to high profile cases of substitution/mislabeling, with many investigations identifying sales of endangered or prohibited species. At the same time, the European Union (EU) has introduced one of the most progressive sets of legislation in order to promote traceability and protect consumers. This study aims to identify shark species that are sold under the commercial term “Galeos” in Greece (which officially designates Mustelus mustelus, M. punctulatus and M. asterias), using DNA barcoding. A total of 87 samples were collected from fishmongers and markets across four cities. A combination of two mitochondrial genes, the cytochrome c oxidase subunit I (COI) and the 16S ribosomal RNA (16S), were used to analyze samples, and species were identified by reference to genetic databases. The results revealed significant differences in patterns of species utilization between cities and retailers. Across the study an extremely high level of mislabeling was identified (56%). This probably relates to some degree of unintentional misidentification and confusion surrounding the designation in Greece, but highlights how consumers are unprotected from incorrect/misleading labels. Over half of products originated from species that are locally listed as threatened by the ICUN red list, and of the mislabeled products, 23% originated from species with prohibitions on landings or CITES listings. This includes large growing sharks with little resemblance to Mustelus spp. and likely demonstrates deliberate substitution. It shows how mislabeled products are providing a route for prohibited/protected sharks to enter the supply chain and be sold to consumers.
T. Pazartzi; S. Siaperopoulou; C. Gubili; S. Maradidou; D. Loukovitis; A. Chatzispyrou; A.M. Griffiths; George Minos; A. Imsiridou. High levels of mislabeling in shark meat – Investigating patterns of species utilization with DNA barcoding in Greek retailers. Food Control 2018, 98, 179 -186.
AMA StyleT. Pazartzi, S. Siaperopoulou, C. Gubili, S. Maradidou, D. Loukovitis, A. Chatzispyrou, A.M. Griffiths, George Minos, A. Imsiridou. High levels of mislabeling in shark meat – Investigating patterns of species utilization with DNA barcoding in Greek retailers. Food Control. 2018; 98 ():179-186.
Chicago/Turabian StyleT. Pazartzi; S. Siaperopoulou; C. Gubili; S. Maradidou; D. Loukovitis; A. Chatzispyrou; A.M. Griffiths; George Minos; A. Imsiridou. 2018. "High levels of mislabeling in shark meat – Investigating patterns of species utilization with DNA barcoding in Greek retailers." Food Control 98, no. : 179-186.
The Eurasian Collared Dove (Streptopelia decaocto) is one of the most successful biological invaders among terrestrial vertebrates. However, little information is available on the genetic diversity of the species. A total of 134 Eurasian Collared Doves from Europe, Asia and the Caribbean (n = 20) were studied by sequencing a 658-bp length of mitochondrial DNA (mtDNA) cytochrome oxidase I (COI). Fifty-two different haplotypes and relatively high haplotype and nucleotide diversities (Hd±SD = 0.843±0.037 and π±SD = 0.026±0.013) were detected. Haplotype Ht1 was particularly dominant: it included 44.03% of the studied individuals, and contained sequences from 75% of the studied countries. Various analyses (FST, AMOVA, STRUCTURE) distinguished 2 groups on the genetic level, designated ‘A’ and ‘B’. Two groups were also separated in the median-joining network and the maximum likelihood tree. The results of the neutrality tests were negative (Fu FS = -25.914; Tajima D = -2.606) and significantly different from zero (P≤0.001) for group A, whereas both values for group B were positive (Fu FS = 1.811; Tajima D = 0.674) and not significant (P>0.05). Statistically significant positive autocorrelation was revealed among individuals located up to 2000 km apart (r = 0.124; P = 0.001). The present results provide the first information on the genetic diversity and structure of the Eurasian Collared Dove, and can thereby serve as a factual and comparative basis for similar studies in the future.
Zoltán Bagi; Evangelos Antonis Dimopoulos; Dimitrios Loukovitis; Cyril Eraud; Szilvia Kusza. MtDNA genetic diversity and structure of Eurasian Collared Dove (Streptopelia decaocto). PLOS ONE 2018, 13, e0193935 .
AMA StyleZoltán Bagi, Evangelos Antonis Dimopoulos, Dimitrios Loukovitis, Cyril Eraud, Szilvia Kusza. MtDNA genetic diversity and structure of Eurasian Collared Dove (Streptopelia decaocto). PLOS ONE. 2018; 13 (3):e0193935.
Chicago/Turabian StyleZoltán Bagi; Evangelos Antonis Dimopoulos; Dimitrios Loukovitis; Cyril Eraud; Szilvia Kusza. 2018. "MtDNA genetic diversity and structure of Eurasian Collared Dove (Streptopelia decaocto)." PLOS ONE 13, no. 3: e0193935.
An updated second linkage map was constructed for the gilthead sea bream, Sparus aurata L., a fish species of great economic importance for the Mediterranean aquaculture industry. In contrast to the first linkage map which mainly consisted of genomic microsatellites (SSRs), the new linkage map is highly enriched with SSRs found in Expressed Sequence Tags (EST-SSRs), which greatly facilitates comparative mapping with other teleosts. The new map consists of 321 genetic markers in 27 linkage groups (LGs): 232 genomic microsatellites, 85 EST-SSRs and 4 SNPs; of those, 13 markers were linked to LGs but were not ordered. Eleven markers (5 SSRs, 5 EST-SSRs and 1 SNP) are not assigned to any LG. The total length of the sex-averaged map is 1769.7 cM, 42% longer than the previously published one, and the number of markers in each LG ranges from 2 to 30. The inter-marker distance varies from 0 to 75.6 cM, with an average of 5.75 cM. The male and female maps have a length of 1349.2 and 2172.1 cM, respectively, and the average distance between markers is 4.38 and 7.05 cM, respectively. Comparative mapping with the three-spined stickleback (Gasterosteus acuulatus) chromosomes and scaffolds showed conserved synteny with 132 S. aurata markers (42.9% of those mapped) having a hit on the stickleback genome
Costas S. Tsigenopoulos; Bruno Louro; Dimitrios Chatziplis; Jacques Lagnel; Emmanouella Vogiatzi; Dimitrios Loukovitis; Rafaella Franch; Elena Sarropoulou; Deborah M. Power; Tomaso Patarnello; Constantinos C. Mylonas; Antonios Magoulas; Luca Bargelloni; Adelino Canario; Georgios Kotoulas. Second generation genetic linkage map for the gilthead sea bream Sparus aurata L. Marine Genomics 2014, 18, 77 -82.
AMA StyleCostas S. Tsigenopoulos, Bruno Louro, Dimitrios Chatziplis, Jacques Lagnel, Emmanouella Vogiatzi, Dimitrios Loukovitis, Rafaella Franch, Elena Sarropoulou, Deborah M. Power, Tomaso Patarnello, Constantinos C. Mylonas, Antonios Magoulas, Luca Bargelloni, Adelino Canario, Georgios Kotoulas. Second generation genetic linkage map for the gilthead sea bream Sparus aurata L. Marine Genomics. 2014; 18 ():77-82.
Chicago/Turabian StyleCostas S. Tsigenopoulos; Bruno Louro; Dimitrios Chatziplis; Jacques Lagnel; Emmanouella Vogiatzi; Dimitrios Loukovitis; Rafaella Franch; Elena Sarropoulou; Deborah M. Power; Tomaso Patarnello; Constantinos C. Mylonas; Antonios Magoulas; Luca Bargelloni; Adelino Canario; Georgios Kotoulas. 2014. "Second generation genetic linkage map for the gilthead sea bream Sparus aurata L." Marine Genomics 18, no. : 77-82.
Genetic variation in seven reared stocks of gilthead sea bream Sparus aurata, originating from Greek commercial farms, was assessed using five polymorphic microsatellite markers and was compared with that of two natural populations from the Ionian and the Adriatic Seas. The total number of alleles per marker ranged from 11 to 19 alleles, and hatchery samples showed the same levels of observed heterozygosity with samples from the wild but substantially smaller allelic diversity and expected heterozygosity. The global genetic differentiation for the cultivated samples was significant as indicated by Fst analysis, which might indicate random genetic drift and inbreeding events operating in the hatcheries. On the contrary, no significant difference was found between the two wild populations. Population pairwise tests between farmed and wild stocks were also significant, with the exception of one hatchery sample, the Central Greece 1, which was not significantly different from the two wild samples perhaps due to its recent use in aquaculture from wild‐caught animals. The UPGMA tree topology grouped the wild samples together with the Central Greece 1 stock, and showed a clear division between wild and farmed sample sets for the six remaining hatchery samples. Knowledge of the genetic variation in S. aurata cultured populations compared with that in the wild ones is essential for setting up appropriate guidelines for the proper monitoring and management of the stocks either under traditional practices or for the implementation of selective breeding programmes.
Dimitrios Loukovitis; Elena Sarropoulou; Emmanouella Vogiatzi; Costas S. Tsigenopoulos; Georgios Kotoulas; Antonios Magoulas; Dimitrios Chatziplis. Genetic variation in farmed populations of the gilthead sea bream Sparus aurata in Greece using microsatellite DNA markers. Aquaculture Research 2011, 43, 239 -246.
AMA StyleDimitrios Loukovitis, Elena Sarropoulou, Emmanouella Vogiatzi, Costas S. Tsigenopoulos, Georgios Kotoulas, Antonios Magoulas, Dimitrios Chatziplis. Genetic variation in farmed populations of the gilthead sea bream Sparus aurata in Greece using microsatellite DNA markers. Aquaculture Research. 2011; 43 (2):239-246.
Chicago/Turabian StyleDimitrios Loukovitis; Elena Sarropoulou; Emmanouella Vogiatzi; Costas S. Tsigenopoulos; Georgios Kotoulas; Antonios Magoulas; Dimitrios Chatziplis. 2011. "Genetic variation in farmed populations of the gilthead sea bream Sparus aurata in Greece using microsatellite DNA markers." Aquaculture Research 43, no. 2: 239-246.
Among vertebrates, teleost fish exhibit a considerably wide range of sex determination patterns that may be influenced by extrinsic parameters. However even for model fish species like the zebrafish Danio rerio the precise mechanisms involved in primary sex determination have not been studied extensively. The zebrafish, a gonochoristic species, is lacking discernible sex chromosomes and the sex of juvenile fish is difficult to determine. Sequential protandrous hermaphrodite species provide distinct determination of the gender and allow studying the sex determination process by looking at the mechanism of sex reversal. This is the first attempt to understand the genetic basis of phenotypic variation for sex determination and body weight in a sequential protandrous hermaphrodite species, the gilthead sea bream (Sparus aurata). This work demonstrates a fast and efficient strategy for Quantitative Trait Loci (QTL) detection in the gilthead sea bream, a non-model but target hermaphrodite fish species. Therefore a comparative mapping approach was performed to query syntenies against two other Perciformes, the European sea bass (Dicentrarchus labrax), a gonochoristic species and the Asian sea bass (Lates calcarifer) a protandrous hermaphrodite. In this manner two significant QTLs, one QTL affecting both body weight and sex and one QTL affecting sex, were detected on the same linkage group. The co-segregation of the two QTLs provides a genomic base to the observed genetic correlation between these two traits in sea bream as well as in other teleosts. The identification of QTLs linked to sex reversal and growth, will contribute significantly to a better understanding of the complex nature of sex determination in S. aurata where most individuals reverse to the female sex at the age of two years through development and maturation of the ovarian portion of the gonad and regression of the testicular area. [Genomic sequences reported in this manuscript have been submitted to GenBank under accession numbers HQ021443–HQ021749.]
Dimitrios Loukovitis; Elena Sarropoulou; Costas S. Tsigenopoulos; Costas Batargias; Antonios Magoulas; Apostolos P. Apostolidis; Dimitrios Chatziplis; Georgios Kotoulas. Quantitative Trait Loci Involved in Sex Determination and Body Growth in the Gilthead Sea Bream (Sparus aurata L.) through Targeted Genome Scan. PLOS ONE 2011, 6, e16599 .
AMA StyleDimitrios Loukovitis, Elena Sarropoulou, Costas S. Tsigenopoulos, Costas Batargias, Antonios Magoulas, Apostolos P. Apostolidis, Dimitrios Chatziplis, Georgios Kotoulas. Quantitative Trait Loci Involved in Sex Determination and Body Growth in the Gilthead Sea Bream (Sparus aurata L.) through Targeted Genome Scan. PLOS ONE. 2011; 6 (1):e16599.
Chicago/Turabian StyleDimitrios Loukovitis; Elena Sarropoulou; Costas S. Tsigenopoulos; Costas Batargias; Antonios Magoulas; Apostolos P. Apostolidis; Dimitrios Chatziplis; Georgios Kotoulas. 2011. "Quantitative Trait Loci Involved in Sex Determination and Body Growth in the Gilthead Sea Bream (Sparus aurata L.) through Targeted Genome Scan." PLOS ONE 6, no. 1: e16599.