In plant cells, ammonium is considered the most convenient nitrogen source for cell metabolism. However, despite ammonium being the preferred N form for microalgae, at higher concentrations, it can be toxic, and can cause growth inhibition. Microalgae’s tolerance to ammonium depends on the species, with various taxa showing different thresholds of tolerability and symptoms of toxicity. In the environment, ammonium at high concentrations represents a dangerous pollutant. It can affect water quality, causing numerous environmental problems, including eutrophication of downstream waters. For this reason, it is important to treat wastewater and remove nutrients before discharging it into rivers, lakes, or seas. A valid and sustainable alternative to conventional treatments could be provided by microalgae, coupling the nutrient removal from wastewater with the production of valuable biomass. This review is focused on ammonium and its importance in algal nutrition, but also on its problematic presence in aquatic systems such as wastewaters. The aim of this work is to provide recent information on the exploitation of microalgae in ammonium removal and the role of ammonium in microalgae metabolism.

The heavy metal pollution in soils and aquatic environments is a serious ecological problem. In the green-microalga Chlorella sorokiniana 211-8K (Chlorophyceae) exposed to ions of lead (Pb) and cadmium (Cd) we studied the metabolic responses to the toxicity of these two heavy metals. Our data indicate that both the pollutants alter the alga cell ultrastructure and its physiological characteristics (growth, photosynthesis, respiration, enzyme activities). The toxic effects of the two metals resulted time-dependent to the exposure. After 24 h of treatment with 250 μM Pb or Cd, photosynthesis was inhibited until to 77 and 86%, however respiration was strongly enhanced up to 300 and 350%, respectively. In the algal cells Pb or Cd exposure induced a reduction in the content of the total chlorophylls and a decrease of the soluble protein levels, significantly compromising the growth, particularly in cultures cadmium-treated. We report data on ultrastructural changes induced by the two heavy metals; they affected overall chloroplast ultrastructure of the alga. Most importantly, the O-acetyl-L-serine(thiol)lyase (OASTL) activity was appreciably increased after only 2 h of Cd exposure, indicating the existence of a link between the metal contamination and cysteine synthesis. Then, Chlorella sorokiniana cells seem to better tolerate high concentrations of Pb while appear to be more sensitive to Cd ions. These results provide some additional information that can lead to better understand consequences of heavy metal poisoning in microalgae.

Phosphorus (P) is a fundamental element for life, playing an integral role in cellular metabolism including energy transfer, nucleic acid synthesis, and membrane structure. This nutrient is critical to the physiological ecology in all photosynthetic organisms including eukaryotic microalgae and cyanobacteria. The review, here presented, delves into the intricate mechanisms governing phosphorus acquisition from the environment, its utilization in plant metabolism, and regulation in these photosynthetic microorganisms. Furthermore, it comprehensively explores the strategies employed by microalgae to cope with phosphorus limitation, such as the activation of high-affinity phosphate transporters and the synthesis of phosphorus storage compounds. On the other hand, the ability to consume abundant phosphate makes microalgae exploitable organisms for environmental remediation processes. The knowledge synthesized in this review contributes to the broader understanding of microalgal physiology, offering insights into the ecological and biotechnological implications of phosphorus assimilation in these microorganisms.

Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved spectrophotometric method to estimate the content of reduced (GSH) and oxidized (GSSG) forms of glutathione in the extremophilic microalga Galdieria phlegrea.

In the last few decades, the increasing interest in microalgae as sources of new biomolecules and environmental remediators stimulated scientists’ investigations and industrial applications. Nowadays, microalgae are exploited in different fields such as cosmeceuticals, nutraceuticals and as human and animal food supplements. Microalgae can be grown using various cultivation systems depending on their final application. One of the main problems in microalgae cultivations is the possible presence of biological contaminants. Fungi, among the main contaminants in microalgal cultures, are able to influence the production and quality of biomass significantly. Here, we describe fungal contamination considering both shortcomings and benefits of fungi-microalgae interactions, highlighting the biological aspects of this interaction and the possible biotechnological applications.

Heavy-metal pollution represents a problem which has been widely discussed in recent years. The biological effects of heavy metals have been studied in both animals and plants, ranging from oxidative stress to genotoxicity. Plants, above all metal-tolerant species, have evolved a wide spectrum of strategies to counteract exposure to toxic metal concentrations. Among these strategies, the chelation and vacuolar sequestration of heavy metals are, after cell-wall immobilization, the first line of defence that prevent heavy metals from interacting with cell components. Furthermore, bryophytes activate a series of antioxidant non-enzymatic and enzymatic responses to counteract the effects of heavy metal in the cellular compartments. In this review, the role of non-protein thiol compounds and antioxidant molecules in bryophytes will be discussed.