Edible coatings and films (ECF) are employed as matrixes for incorporating antimicrobial nanoparticles (NPs), and then they are applied on the fruits and vegetables to prolong shelf life and enhance storage quality. This paper provides a comprehensive review on the preparation, antimicrobial properties and mechanisms, surface and physical qualities of ECF containing antimicrobial NPs, and its efficient application to vegetables and fruits as well. Following an introduction on the properties of the main edible coating materials, the preparation technologies of ECF with NPs are summarized. The antimicrobial activity of ECF with NPs against the tested microorganism was observed by many researchers. This might be mainly due to the electrostatic interaction between the cationic polymer or free metal ions and the charged cell membrane, the photocatalytic reaction of NPs, the detachment of free metal ion, and partly due to the antimicrobial activity of edible materials. Moreover, their physical, mechanical and releasing properties are discussed in detail, which might be influenced by the concentration of NPs. The preservation potential on the quality of fruits and vegetables indicates that various ECF with NPs might be used as the ideal materials for food application. Following the introduction on these characteristics, an attempt is made to predict future trends in this field.

In this investigation, the effect of different concentrations of titanium dioxide (TiO₂) nanoparticles (NPs) on the structure and antimicrobial activity of chitosan-based coating films was examined. Analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed that the modified TiO₂ NPs were successfully dispersed into the chitosan matrix, and that the roughness of the chitosan-TiO₂ nanocomposites were significantly reduced. Moreover, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses indicated that the chitosan interacted with TiO₂ NPs and possessed good compatibility, while a thermogravimetric analysis (TGA) of the thermal properties showed that the chitosan-TiO₂ nanocomposites with 0.05% TiO₂ NPs concentration had the best thermal stability. The chitosan-TiO₂ nanocomposite exhibited an inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus. This antimicrobial activity of the chitosan-TiO₂ nanocomposites had an inhibition zone ranging from 9.86 ± 0.90 to 13.55 ± 0.35 (mm). These results, therefore, indicate that chitosan-based coating films incorporated with TiO₂ NPs might become a potential packaging system for prolonging the shelf-life of fruits and vegetables.

Chitosan nanoparticles (CSNPs) have attracted wide interest; however, there has been no substantial information about a direct comparison of the antimicrobial activity of CSNPs on bacteria and fungi. Thus, in this study, simple, economically feasible CSNPs were synthesized and assessed for their antimicrobial activity. This investigation indicated that the coordination inducing effect of CSNPs could dissociate the tryptophan (Trp) and tyrosine (Tyr) residue groups on the peptide chain of the bovine serum albumin (BSA) molecule, thereby increasing the absorption intensity. The growth of E. coli and S. aureus could be completely inhibited when the concentration of CSNPs in the solution was higher than 0.6 mg/mL. The CSNPs showed more potent antibacterial activity against Gram-negative bacteria (E. coli) than against Gram-positive bacteria (S. aureus). In addition, the CSNPs were effective at initiating cellular leakage of fungal mycelia and damping off fungal pathogens, and their antifungal effects were stronger on P. steckii than on A. oryzae. Furthermore, the antimicrobial activity of the CSNPs was found to be more effective against bacteria than against fungi. This study thus ascertained the antimicrobial activity of synthesized CSNPs against different microorganisms, as well as their different degrees of inhibition.

Blueberries are a rich source of health-promoting compounds such as vitamins and anthocyanins and show a high antioxidant capacity. Thus, considerable commercial and scientific interest exists in prolonging its postharvest life to meet the year-round demand for this fruit. In this investigation, the effect of a chitosan-based edible coating, as well as a chitosan-based edible coating containing nanosized titanium dioxide particles (CTS-TiO₂), on the postharvest quality of blueberry fruit quality was evaluated during storage at 0 °C. The blueberries were treated with a chitosan coating (CTS) and a CTS-TiO₂ composite, respectively. The most suitable chitosan and nano-TiO₂ fraction concentrations to be incorporated in the coating formulation were prepared based on the wettability of the corresponding coating solutions. Changes in firmness, total soluble solids (TSS), titratable acidity (TA), ascorbic acid (VC), malondialdehyde (MDA), polyphenol oxidase (PPO), and peroxidase (POD) activities, anthocyanins, flavonoids, total phenolic content, and microbiological analysis were measured and compared. This combined treatment prevented product corruption. Compared with CTS, the CTS-TiO₂ composite coating application effectively slowed down the decrease in firmness, TSS, VC, and TA in the blueberries. Additionally, changes in the total polyphenol, anthocyanin, and flavonoid contents and the antioxidant capacity of CTS-TiO₂ composite coating blueberry fruits were delayed. Therefore, these results indicated that the chitosan/nano-TiO₂ composite coating could maintain the nutrient composition of blueberries while playing a significant role in preserving the quality of fruit at 0 °C.

Postharvest pathogens such as C. gloeosporioides (MA), C.oxysporum (ME) and P. steckii (MF) are the causal agents of disease in mangoes. This paper presents an in vitro investigation into the antifungal effect of a chitosan (CTS)/nano-titanium dioxide (TiO₂) composite coating against MA, ME and MF. The results indicated that, the rates of MA, ME and MF mortality following the single chitosan treatment were 63.3%, 84.8% and 43.5%, respectively, while the rates of mycelial inhibition were 84.0%, 100% and 25.8%, respectively. However, following the addition of 0.5% nano-TiO₂ into the CTS, both the mortality and mycelial inhibition rates for MA and ME reached 100%, and the mortality and mycelial inhibition rate for MF also increased significantly, reaching 75.4% and 57.3%, respectively. In the MA, the dry weight of mycelia after the CTS/0.5% nano-TiO₂ treatment decreased by 36.3% in comparison with the untreated group, while the conductivity value was about 1.7 times that of the untreated group, and the protein dissolution rate and extravasation degree of nucleic acids also increased significantly. Thus, this research revealed the potential of CTS/nano-TiO₂ composite coatings in the development of new antimicrobial materials.