After my M.Sc. graduation at the Faculty of Science at Charles University, I moved to the Institute of Microbiology of the CAS. Therein, during my Ph.D. study, I participated in projects concerning a phenomenon of stress granules. We were one of those who showed that stress granules are evolutionarily conserved and opened up a possibility to use the yeast model organism for their study. Obtaining a Ph.D. degree, I moved to the University of Heidelberg in Germany. The lab I joined is one of the leading groups in the research of the biogenesis and quality control of proteins. At that time, I was focused on a problematics of small heat shock proteins and their role in cellular homeostasis and stress response. Finally, I returned to the Institute of Microbiology of the CAS. My contemporary projects concern an investigation of signal transduction and translation control in mammalian cells.