As heterotrophic respiration (R_H) has great potential to increase atmospheric CO₂ concentrations, it is important to understand warming effects on R_H for a better prediction of carbon–climate feedbacks. However, it remains unclear how R_H responds to warming in subtropical forests. Here, we carried out trenching alone and trenching with warming treatments to test the climate warming effect on R_H in a subtropical forest in southwestern China. During the measurement period, warming increased annual soil temperature by 2.1 °C and increased annual mean R_H by 22.9%. Warming effect on soil temperature (WE_T) showed very similar pattern with warming effect on R_H (WE_RH), decreasing yearly. Regression analyses suggest that WE_RH was controlled by WE_T and also regulated by the soil water content. These results showed that the decrease of WE_RH was not caused by acclimation to the warmer temperature, but was instead due to decrease of WE_T. We therefore suggest that global warming will accelerate soil carbon efflux to the atmosphere, regulated by the change in soil water content in subtropical forests.

This study examines nitrogen transformation mechanisms and compost quality in mesophilic aerobic composting of wheat straw, utilizing cow manure as a co-substrate to promote sustainable agricultural waste management. Two composting systems were established: group A (control) and group B (10% cow manure addition by wet weight). The addition of cow manure accelerated early organic matter decomposition and increased total nitrogen retention in group B. Nitrogen losses occurred primarily via ammonia volatilization during the initial and final composting stages, while functional gene analysis revealed enhanced ammonification and nitrification in both systems. Microbial community analysis showed that cow manure addition promoted nitrogen-fixing bacteria in the early phase and fungi associated with complex organic degradation in later stages. These findings underscore the potential of cow manure to enhance compost maturity, improve nitrogen efficiency, and support the development of sustainable composting practices that contribute to resource conservation.